Article
Pharmacology & Pharmacy
Manuela Mulazzi, Elisabetta Campodoni, Giada Bassi, Monica Montesi, Silvia Panseri, Francesca Bonvicini, Giovanna Angela Gentilomi, Anna Tampieri, Monica Sandri
Summary: This study designed and investigated a medicated osteoinductive and bioresorbable bone graft to control antibiotic drug release in situ for the eradication or prevention of infection, while simultaneously repairing bone defects. Microbiological and in vitro tests showed that the MgHA/Coll scaffolds were safe and effective in prolonging therapy duration, promising results for the prevention of bone defect-related infections in orthopedic surgeries.
Article
Chemistry, Applied
Danny Zanardo, Giulia Forghieri, Sebastiano Tieuli, Elena Ghedini, Federica Menegazzo, Alessandro Di Michele, Giuseppe Cruciani, Michela Signoretto
Summary: This study investigated two silica-based materials with different porosity levels as supports for titanium dioxide in gas-phase CO2 photoreduction. The TiO2-SiO2 composites demonstrated comparable catalytic activity to pure TiO2, despite a low fraction of photoactive phase, due to improved light harvesting and reagents adsorption.
Review
Biochemistry & Molecular Biology
Fengbo Sun, Xiaodan Sun, Hetong Wang, Chunxu Li, Yu Zhao, Jingjing Tian, Yuanhua Lin
Summary: This review systematically summarizes the research progress of 3D-printed, PLGA-based scaffolds, introduces the properties of the modified components, analyzes the influence of structure and printing method change in the printing process, illustrates the advantages and disadvantages of their applications with examples, and finally discusses the limitations and future development direction of current 3D-printed, PLGA-based materials for bone tissue repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yuan Sun, Xiaona Jia, Qing Meng
Summary: This study investigated the fabrication of nanofibrous scaffolds by blending recombinant spider silk proteins (R1SR2 and NR1SR2C) with Poly(lactic-co-glycolic) Acid (PLGA). The presence of N- and C-termini of MiSp contributed to the reduction of nanofiber diameter, increased porosity and glass transition temperatures of the scaffolds, as well as improved hydrophilicity and ultimate strain. In cell culture assays, HBMSCs grown on NR1SR2C/PLGA (20/80) scaffolds showed enhanced proliferative and adhesive abilities compared to those on pure PLGA scaffolds. These findings suggest promising applications of recombinant MiSp/PLGA blend nanofibrous scaffolds, particularly NR1SR2C/PLGA (20/80), in bone tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Hu Qian, Ting Lei, Long Hua, Yu Zhang, Dongyu Wang, Jiangyu Nan, Wenbin Liu, Yan Sun, Yihe Hu, Pengfei Lei
Summary: In this study, a novel scaffold composed of gelatin methacryloyl (GelMA) hydrogel and poly(lactic co-glycolic acid) (PLGA) microspheres encapsulating vancomycin (Van) was developed for one-stage repair of infected bone defects (IBDs). The scaffold exhibited sustained release of Van for over 2 weeks and demonstrated good biocompatibility, bacteriostasis, and osteointegration properties, showing great potential for clinical application. The construction of this scaffold provides new insights into the development of orthopaedic implants and offers a novel approach for the treatment of IBDs.
BIOACTIVE MATERIALS
(2023)
Article
Engineering, Biomedical
Xulin Hu, Hu Li, Liang Qiao, Shuhao Yang, Haoming Wu, Chao Peng, Yamei Zhang, Hai Lan, Hua Yang, Kainan Li
Summary: Human bones consist of cortical and cancellous bone, with cancellous bone being porous and cortical bone being dense. Porous ceramics are a promising area of research in bone tissue engineering due to their similarity to human bone. 3D printing of ceramics is a popular method for fabricating porous scaffolds that meet the requirements of cancellous bone strength and complex shapes. In this study, beta-tricalcium phosphate (beta-TCP)/titanium dioxide (TiO2) porous ceramics scaffolds were successfully fabricated using 3D gel-printing sintering, with a uniform porous structure and enhanced compressive strength. The scaffolds showed no toxicity and exhibited excellent cell adhesion and proliferation, making them a potential candidate for orthopedic and traumatology repair scaffolds.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Polymer Science
Rittichai Sangkatip, Wipoo Sriseubsai, Kunlanan Kiatkittipong, Kaona Jongwuttanaruk
Summary: The research aimed to prepare a biomedical scaffold material using gelatin and titanate, and determine the optimal mixture for maximum tensile strength through a mixture design. The findings showed that the scaffold formed with titanate had higher tensile strength, and the strength could be further improved through appropriate mixture design.
Article
Medicine, Research & Experimental
Juliana C. Quarterman, Pornpoj Phruttiwanichakun, Douglas C. Fredericks, Aliasger K. Salem
Summary: Osteoarthritis patients may require revision surgery after joint replacement due to prosthetic loosening. Coating with zoledronic acid (ZA) has been shown to enhance implant osseointegration, as evidenced by in vitro and in vivo experiments.
MOLECULAR PHARMACEUTICS
(2022)
Article
Materials Science, Biomaterials
Akihiro Furuhashi, Yunia Dwi Rakhmatia, Yasunori Ayukawa, Kiyoshi Koyano
Summary: The aim of this study was to investigate the use of titanium membranes incorporated in poly (lactic-co-glycolic acid) (PLGA) for guided bone regeneration. The study found that the titanium membranes significantly affected the mechanical and chemical properties, with higher release of fluvastatin in the PLGA-FS group. Micro-CT, morphometrical analysis, and histological observations showed that the TM-PLGA-FS group significantly enhanced bone formation.
REGENERATIVE BIOMATERIALS
(2022)
Article
Polymer Science
Humberto Alejandro Monreal Romero, Teresa Perez Pinon, Diana Sagarnaga, Raquel Duarte Rico, Alfredo Nevarez Rascon, Carlos Alberto Martinez Perez, Dagoberto Perez Pinon, Juan Pablo Flores de los Rios, Mario Sanchez Carrillo, Jose Guadalupe Chacon-Nava
Summary: In this study, titanium dioxide scaffolds were successfully synthesized using the sol-gel method. The scaffolds were formed by using titanium isopropoxide as a precursor and a polymeric network of galactopyranose as a template. The synthesized scaffolds have diameters of 50 nm and porosity ranging from 0.3 to 10 nm. XRD analysis confirmed the formation of crystalline phases in the TiO2 scaffold at 700 degrees C. The use of biological polymers as templates provides a low-cost and efficient approach for the synthesis of new materials.
Article
Engineering, Biomedical
Sanaz Ghasemi, Hamed Ghomi
Summary: In this study, composite scaffolds of bredigite/titanium dioxide were prepared by gelcasting method, coated with chitosan polymer for improved mechanical, biological, and antibacterial properties. Characterization analysis showed improved compressive strength and reduced porosity with the addition of titanium dioxide, as well as significant enhancement in compressive strength and reduced porosity with chitosan coating. Antimicrobial testing revealed inhibition zones against both Gram-negative and Gram-positive bacteria. Cell compatibility testing showed no toxicity and favorable growth and adhesion of MG63 bone cells. Therefore, the scaffold developed in this study shows promise for bone tissue engineering applications.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2021)
Article
Engineering, Biomedical
Viviane Staendert, Kai Borcherding, Nicole Bormann, Gerhard Schmidmaier, Ingo Grunwald, Britt Wildemann
Summary: The study demonstrates the creation of a mechanically stable porous structure on a titanium surface and embedding of silver particles to enhance osteointegration and provide antibacterial properties through preoperative loading of antibiotics. The novel surface modification shows good biocompatibility, antibacterial effects, and support for osteointegration without negative impact of the released drug.
BIOACTIVE MATERIALS
(2021)
Article
Nanoscience & Nanotechnology
Aline O. Pereira, Isabella M. I. Lopes, Thiago R. Silva, Thaila Q. Correa, Rafaella T. Paschoalin, Natalia M. Inada, Ievgeniia Iermak, Francisco van Riel Neto, Juliana C. Araujo-Chaves, Alexandre Marletta, Jose R. Tozoni, Luiz Henrique C. Mattoso, Vanderlei S. Bagnato, Iseli L. Nantes-Cardoso, Osvaldo N. Oliveira Jr, Patricia T. Campana
Summary: This research demonstrates that nanofibers can be utilized as an alternative method for bacterial inactivation, instead of UV and blue irradiation. The use of PLGA nanofiber scaffolds can effectively kill Staphylococcus aureus and Escherichia coli bacteria at lower light intensities. The enhanced effect of PLGA scaffolds is attributed to their nanofiber structures, which could pave the way for a more cost-effective and environmentally friendly surface sterilization method.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Physical
Fajun Chen, Jian Han, Zeyong Guo, Chongjing Mu, Chuandi Yu, Zhibo Ji, Lei Sun, Yujuan Wang, Junfeng Wang
Summary: To address the challenge of infectious bone defects in the clinical setting, antibacterial scaffolds were fabricated using a silver nanoparticle/poly lactic-co-glycolic acid (AgNP/PLGA) material via direct ink writing (DIW) 3D printing technique. The scaffolds exhibited uniform surface pores, enhanced mechanical strength, and continuous release of silver ions, promoting hydroxyapatite growth. The scaffolds showed excellent antibacterial properties against Staphylococcus aureus and Escherichia coli, and demonstrated excellent biocompatibility with osteoblast precursor cells. These findings highlight the potential application of 3D-printed AgNPs/PLGA scaffolds in bone tissue engineering.
Article
Biochemistry & Molecular Biology
Iwona Pudelko, Anna Moskwik, Konrad Kwiecien, Sven Kriegseis, Malgorzata Krok-Borkowicz, Karolina Schickle, Dorota Ochonska, Piotr Dobrzynski, Monika Brzychczy-Wloch, Jesus Gonzalez-Julian, Elzbieta Pamula
Summary: Implant-related infections are a global challenge, and new solutions are being investigated to overcome conventional therapy failures. In this study, porous zirconia scaffolds were manufactured and coated with antibiotic-loaded polymer nanoparticles to enhance bioactivity and achieve antibacterial effects. The scaffolds exhibited high encapsulation efficiency and drug loading, and showed excellent cytocompatibility and antimicrobial properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Minh Khai Le Thieu, Sabine Stoetzel, Maryam Rahmati, Thaqif El Khassawna, Anders Verket, Javier Sanz-Esporrin, Mariano Sanz, Jan Eirik Ellingsen, Havard Jostein Haugen
Summary: The study evaluated osteogenic markers and alveolar ridge profile changes in noncontained bone defects treated with guided bone regeneration using a nonresorbable TiO2 block. Results indicated contact osteogenesis with TiO2 block or DBBM particulates, and potential for bone formation beyond 12 weeks. Alveolar profile data showed sustained lateral bone augmentation with TiO2 block and collagen membrane compared to other treatment options.
CLINICAL IMPLANT DENTISTRY AND RELATED RESEARCH
(2023)
Review
Dentistry, Oral Surgery & Medicine
Hongji Yan, Gustavo De Deus, Ida Marie Kristoffersen, Elisabeth Wiig, Janne Elin Reseland, Gaute F. Johnsen, Emmanuel J. N. L. Silva, Havard J. Haugen
Summary: The conventional treatment for irreversibly inflamed or necrotic teeth is root canal treatment or apexification. However, regenerative endodontics using the cell-homing technique shows promising results that can be translated into clinical practice. The cell homing technique is based on endogenous stem cells' capacity to regenerate tissue, and its success criteria have been defined by the American Association of Endodontists. This article provides an overview of vital pulp tissue and various strategies to promote regeneration of damaged pulp tissue, with a focus on the cell homing technique.
JOURNAL OF ENDODONTICS
(2023)
Article
Nanoscience & Nanotechnology
Gaelle Desante, Iwona Pudelko, Malgorzata Krok-Borkowicz, Elzbieta Pamula, Philipp Jacobs, Alicja Kazek-Kesik, Jonas Niessen, Rainer Telle, Jesus Gonzalez-Julian, Karolina Schickle
Summary: This study proposes a novel biomimetic calcium phosphate (CaP) coating decorated with antibiotic-loaded nanoparticles on inert ceramic implants to enhance bioactivity and provide antibacterial effect. The coprecipitation method was found to achieve a favorable and homogenous distribution of nanoparticles within the CaP coating. The coating, consisting of hydroxyapatite and octacalcium phosphate, showed controlled release of antibiotics and inhibited the growth of bacteria.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Biomedical
A. C. Moore, M. G. Hennessy, L. P. Nogueira, S. J. Franks, M. Taffetani, H. Seong, Y. K. Kang, W. S. Tan, G. Miklosic, R. El Laham, K. Zhou, L. Zharova, J. R. King, B. Wagner, H. J. Haugen, A. Munch, M. M. Stevens
Summary: This paper reports on the development of an engineered poroelastic material that begins to approach physiological performance. The material achieved a mean peak fluid load fraction of 68%, displayed consistency with mixture theory, and demonstrated cytocompatibility. This work creates a foundation for designing poroelastic cartilage implants and developing scaffold systems to study chondrocyte mechanobiology and tissue engineering.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Song Chen, Dachuan Liu, Le Fu, Bing Ni, Zongkun Chen, Jennifer Knaus, Elena V. Sturm, Bohan Wang, Havard Jostein Haugen, Hongji Yan, Helmut Coelfen, Bin Li
Summary: This study reports the synthesis and characterization of an iron-bearing amorphous calcium phosphate, along with its potential application in biomineralization and as a starting material for acid-resistant high-performance bioceramics. The prepared material shows high stability and biocompatibility in aqueous media, and the ceramics composed of iron-calcium phosphates demonstrate enhanced acid resistance and hardness.
ADVANCED MATERIALS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Ke Ning, Fang Yang, Ewald Bronkhorst, Jan Ruben, Liebert Nogueira, Havard Haugen, Bas Loomans, Sander Leeuwenburgh
Summary: This study investigated the fatigue behavior and aging process of self-healing composites containing microcapsules. The results showed that the self-healing composites exhibited improved fatigue resistance compared to the composites without microcapsules.
Article
Chemistry, Applied
Agnieszka Kumorkiewicz-Jamro, Renata Gorska, Malgorzata Krok-Borkowicz, Katarzyna Reczynska-Kolman, Przemyslaw Mielczarek, Lukasz Popenda, Aneta Sporna-Kucab, Anna Tekieli, Elzbieta Pamula, Slawomir Wybraniec
Summary: Atriplex hortensis var. rubra L. extracts from different parts of the plant were analyzed for betalainic profiles and showed strong antioxidant activity. Celosianin and amaranthin were identified as the most potent compounds. The chemical structure of celosianin was elucidated for the first time. Furthermore, the extracts and purified pigments did not induce cytotoxicity and effectively protected cells from oxidative stress-induced cell death and apoptosis.
Article
Polymer Science
Lidia Ciolek, Malgorzata Krok-Borkowicz, Arkadiusz Gasinski, Monika Biernat, Agnieszka Antosik, Elzbieta Pamula
Summary: The usefulness of bioactive glasses enriched with SrO or ZnO in the manufacturing of chitosan-based scaffolds was evaluated. The study confirmed that the biological performance of chitosan/bioglass composites can be controlled by adjusting the composition and heat treatment parameters of bioglasses.
Article
Green & Sustainable Science & Technology
Konrad Kwiecien, Monika Brzychczy-Wloch, Elzbieta Pamula
Summary: Resistance to antibiotics is a major threat to humanity. Various targeted drug delivery systems, such as biopolymeric micro- or nanoparticles, electrospun nanofibers, or self-emulsifying drug delivery systems, are being explored to reduce antibiotic usage. One approach is to modify the chemical character of hydrophilic antibiotics using ion pairing with cationic or ionic surfactants, enhancing encapsulation efficiency and providing sustainable release. However, the presence of surfactants may increase toxicity, and the mechanisms behind improved antibacterial properties are not fully understood.
SUSTAINABLE MATERIALS AND TECHNOLOGIES
(2023)
Meeting Abstract
Cell & Tissue Engineering
Anna M. Tryba, Malgorzata Malgorzata Krok-Borkowicz, Michal Kula, Natalia Piergies, Mateusz Marzec, Erik Wegener, Justyna Fraczyk, Rainer Jordan, Beata Kolesinska, Dieter Scharnweber, Czeslawa Paluszkiewicz, Elzbieta Pamula
TISSUE ENGINEERING PART A
(2023)
Meeting Abstract
Cell & Tissue Engineering
Agata Flis, Konrad Kwiecien, Izabela Misiolek, Filip Koper, Tomasz Swiergosz, Wiktor Kasprzyk, Elzbieta Pamula
TISSUE ENGINEERING PART A
(2023)
Article
Dentistry, Oral Surgery & Medicine
Lisa Printzell, Janne Elin Reseland, Nina Frederike Jeppesen Edin, Jan Eirik Ellingsen, Hanna Tiainen
Summary: The influence of radiation backscatter from titanium on DNA damage and migration capacity of human osteoblasts and mesenchymal stem cells was investigated. Higher doses of radiation and backscatter from titanium increased DNA damage, with mesenchymal stem cells being more affected. High doses of radiation inhibited cell migration on titanium, while low doses did not affect migration capacity.
CLINICAL ORAL INVESTIGATIONS
(2023)
Article
Polymer Science
Klaudia Ordon, Piotr Szatkowski, Wojciech Piekarczyk, Elzbieta Pamula, Kinga Pielichowska
Summary: Novel polyurethane-based materials were synthesized using a two-step process with poly(epsilon-caprolactone) diol (PCL) and 1,3-propanediol/starch (PDO/ST) systems as chain extenders/cross-linkers. Poly(ethylene glycol)/starch (PEG/ST) system as a phase change material (PCM) decreased the maximum setting temperature, and hydroxyapatite (HAp) was used as a bioactive nanofiller. The synthesized polyurethanes showed good energy storage effect and potential application for bone cements.
Review
Engineering, Biomedical
Zahra Miri, Silvia Fare, Qianli Ma, Havard J. Haugen
Summary: Polyurethanes (PUs) have promising properties for biomedical applications due to their ability to achieve specific desirable properties by changing their chemical structure. PUs attract attention from different medical fields due to their various properties. This review highlights the properties of PUs, such as biodegradability, biostability, shape memory, and improved antibacterial activity, and focuses on their applications in tissue engineering, cardiovascular medicine, drug delivery, and wound healing.
PROGRESS IN BIOMEDICAL ENGINEERING
(2023)
Article
Polymer Science
Martyna Gawel, Patrycja Domalik-Pyzik, Timothy E. L. Douglas, Katarzyna Reczynska-Kolman, Elzbieta Pamula, Kinga Pielichowska
Summary: This study proposes and investigates new scaffolds based on whey protein isolate (WPI) and chitosan (CS) for osteochondral tissue repair. The optimal WPI/CS hydrogels were chosen based on various evaluation criteria, and it was found that the hydrogels with CS incorporated in powder form exhibited superior mechanical properties and those with CS incorporated after dissolution showed enhanced wettability. The introduction of CS powder promoted apatite formation and the CS powder modified WPI hydrogels showed good cytocompatibility, indicating their potential for osteochondral tissue repair.
Article
Materials Science, Biomaterials
Mohammadreza Kasravi, Alireza Yaghoobi, Tahereh Tayebi, Mahsa Hojabri, Abdolkarim Talebi Taheri, Fatemeh Shirzad, Bahram Jambar Nooshin, Radman Mazloomnejad, Armin Ahmadi, Fatemeh A. Tehrani, Ghasem Yazdanpanah, Mohammad Hadi Farjoo, Hassan Niknejad
Summary: As a promising approach in translational medicine, the decellularization of discarded livers to produce bioscaffolds that support recellularization has potential in overcoming the limitations of conventional liver transplantation. In this study, the researchers investigated the use of matrix metalloproteinase (MMP) inhibition to preserve the extracellular matrix (ECM) during liver decellularization. The results demonstrated that the application of an MMP inhibitor significantly improved the preservation of ECM components and mechanical properties of the bioscaffolds, which supported cell viability and function in vitro. The study also confirmed that the MMP inhibition led to the inhibition of MMP2 and MMP9, providing a novel method to enhance ECM preservation during liver decellularization.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Mohammadsadegh Nadimifar, Weiguang Jin, Clara Coll-Satue, Gizem Bor, Paul Joseph Kempen, Ali Akbar Moosavi-Movahedi, Leticia Hosta-Rigau
Summary: This study presents a metal-phenolic self-assembly approach that can prepare nanoparticles fully made of hemoglobin. The nanoparticles exhibit good oxygen binding and releasing capabilities.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jyoti Kumari, Roel Hammink, Jochem Baaij, Frank A. D. T. G. Wagener, Paul H. J. Kouwer
Summary: Fibrosis is the formation of fibrous connective tissue in response to injury, leading to organ dysfunction. A novel hybrid hydrogel combining synthetic polyisocyanide with hyaluronic acid has been developed, showing strong antifibrotic properties.
BIOMATERIALS ADVANCES
(2024)
Letter
Materials Science, Biomaterials
Melissa Machado Rodrigues, Cristian Padilha Fontoura, Charlene Silvestrin Celi Garcia, Sandro Tomaz Martins, Joao Antonio Pegas Henriques, Carlos Alejandro Figueroa, Mariana Roesch Ely, Cesar Aguzzoli
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jessica Polak, David Sachs, Nino Scherrer, Adrian Suess, Huan Liu, Mitchell Levesque, Sabine Werner, Edoardo Mazza, Gaetana Restivo, Mirko Meboldt, Costanza Giampietro
Summary: Human skin equivalents (HSEs) play a crucial role in tissue engineering. This study introduces a 3D-printed culture insert to apply a static radial constraint on HSEs and examines its effects on tissue characteristics. The results show that the diameter of the culture insert significantly influences tissue contraction, fibroblast and matrix organization, keratinocyte differentiation, epidermal stratification, and basement membrane formation. This study provides important insights for the design of skin tissue engineering.
BIOMATERIALS ADVANCES
(2024)
Review
Materials Science, Biomaterials
Shiliang Chen, Tianming Du, Hanbing Zhang, Jing Qi, Yanping Zhang, Yongliang Mu, Aike Qiao
Summary: This paper reviewed the primary methods for improving the overall properties of biodegradable zinc stents. It discussed the mechanical properties, degradation behavior, and biocompatibility of various improvement strategies. Alloying was found to be the most common, simple, and effective method for improving mechanical properties. Deformation processing and surface modification further improved the mechanical properties and biological activity of zinc alloys. Meanwhile, structural design could endow stents with special properties. Manufacturing zinc alloys with excellent properties and exploring their interaction mechanism with the human body are areas for future research.
BIOMATERIALS ADVANCES
(2024)