Article
Biochemistry & Molecular Biology
Qun Liu, Bo Li, Yan Li, Xiaodeng Yang, Congde Qiao, Wei Hu, Mingxia Liu
Summary: pH-sensitive and amphiphilic chitosan derivatives were synthesized and studied for their rheological properties, showing that high degree of substitution and pH near the isoelectric points corresponded to low viscosity and viscoelasticity. Strong networks formed at high concentrations, and the role of Ca2+ varied depending on concentration levels. Molecular dynamics simulations were also used to examine intermolecular hydrogen bonds.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Zhi Zhao, Jinyu Han, Shangen Xu, Zheng Jin, Tan Hui Yin, Kai Zhao
Summary: This report presents the encapsulation of amoxicillin in N-2-Hydroxypropyl trimethyl ammonium chloride chitosan and N,O-carboxymethyl chitosan nanoparticles for biomedical applications. The synthesized nanoparticles demonstrated sustained drug release, good cytocompatibility and broad-spectrum antimicrobial activity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Feng Cheng, Lei Xu, Jiliang Dai, Xiaotong Yi, Jinmei He, Hongbin Li
Summary: In this study, a biodegradable composite sponge was designed and fabricated, which exhibited excellent antibacterial and antiadhesion properties. It is believed that this composite sponge can serve as a new medical device for tissue repair and prevention of postoperative adhesion.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Pharmacology & Pharmacy
Adriana Trapani, Stefania Cometa, Elvira De Giglio, Filomena Corbo, Roberta Cassano, Maria Luisa Di Gioia, Sonia Trombino, Md Niamat Hossain, Sante Di Gioia, Giuseppe Trapani, Massimo Conese
Summary: A widely investigated approach to bypass the blood brain barrier is represented by the intranasal delivery of therapeutic agents exploiting the olfactory or trigeminal connections nose-brain. As for Parkinson's disease (PD), characterized by dopaminergic midbrain neurons degeneration, currently there is no disease modifying therapy. Although several bio-nanomaterials have been evaluated for encapsulation of neurotransmitter dopamine (DA) or dopaminergic drugs in order to restore the DA content in parkinsonian patients, the premature leakage of the therapeutic agent limits this approach. To tackle this drawback, we undertook a study where the active was linked to the polymeric backbone by a covalent bond. Thus, novel nanoparticles (NPs) based on N,O-Carboxymethylchitosan-DA amide conjugate (N,O-CMCS-DA) were prepared by the nanoprecipitation method and characterized from a technological view point, cytotoxicity and uptake by Olfactory Ensheating Cells (OECs). Thermogravimetric analysis showed high chemical stability of N,O-CMCS-DA NPs and X-ray photoelectron spectroscopy evidenced the presence of amide linkages on the NPs surface. MTT test indicated their cytocompatibility with OECs, while cytofluorimetry and fluorescent microscopy revealed the internalization of labelled N,O-CMCS-DA NPs by OECs, that was increased by the presence of mucin. Altogether, these findings seem promising for further development of N,O-CMCS-DA NPs for nose-to-brain delivery application in PD.
Article
Biochemistry & Molecular Biology
Emad Naseri, Christopher Cartmell, Matthew Saab, Russell G. Kerr, Ali Ahmadi
Summary: This study investigated a novel hybrid biomaterial ink consisting of starch and N,O-carboxymethyl chitosan (NOCC), used for fabricating controlled release biodegradable wound dressing scaffolds via a low-temperature solvent-free 3D printing technique. By altering the ratio of NOCC to starch in the biomaterial ink, drug release and inhibition zone size can be controlled. Increasing NOCC accelerated drug release and led to larger zones of inhibition, attributed to enhanced hydrophilicity of NOCC-dominated scaffolds.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Ashiq Hussain Pandit, Safiya Nisar, Khalid Imtiyaz, Masood Nadeem, Nasreen Mazumdar, M. Moshahid Alam Rizvi, Sharif Ahmad
Summary: The synthesized N,O-CMCS/MAGG hydrogel showed promising applications in localized delivery of anticancer drugs. The hydrogel exhibited pH-responsive swelling behavior, good mechanical properties, excellent self-healing and thixotropic properties due to the reversible Schiff base linkages. The Dox-loaded hydrogel demonstrated significant killing effect against breast cancer cells, indicating its potential in anticancer drug delivery.
Article
Food Science & Technology
Ngoc Nguyen, Trieu Nguyen, Phu Le Hong, Thi Kieu Hanh Ta, Bach Thang Phan, Hanh Nguyen Thi Ngoc, Hang Phung Thi Bich, Nhi Dinh Yen, Toi Vo Van, Hiep Thi Nguyen, Diep Tran Thi Ngoc
Summary: This study investigated the efficacy of preserving Ngoc Linh ginseng using coatings of chitosan derivatives and polyvinyl alcohol (PVA) solutions. The findings showed that the coated ginseng samples performed better in terms of preservation compared to the non-coated samples, with the NOCC-PVA solution exhibiting a better effect than the COS-PVA solution.
Article
Biotechnology & Applied Microbiology
Li Xu, Zhanying Ren, Guolin Li, Danni Xu, Jiaqian Miao, Jingxuan Ju, Xuan Mo, Xianghui Wang, Hongping Deng, Min Xu
Summary: A liver-targeting gadolinium-based MRI contrast agent was developed using galactose functionalized o-carboxymethyl chitosan, which exhibited higher hepatocyte uptake, excellent biocompatibility, and better T1-weighted signal enhancement in the liver. The agent showed good safety in vivo at a specific dose.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Katarzyna Lewandowska, Marta Szulc
Summary: The study characterized mixtures based on carboxymethyl chitosan (CMCh) and poly(N-vinylpyrrolidone) (PVP) using various analytical techniques. The results showed that CMCh/PVP mixtures had higher apparent viscosity and good miscibility in aqueous solution.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Physical
Min Yuan, Zeping Wang, Yifan Rao, Ying Wang, Bo Gao, Jiabing Yu, Hui Li, Xianping Chen
Summary: This paper reports an efficient, green, and low-cost method to produce O/N/S co-doped hierarchically porous graphene using laser direct writing on wood. The resulting materials have a three-dimensional framework, large specific surface area, and rich heteroatoms doping, leading to excellent electrochemical performance for microsupercapacitors.
Article
Biochemistry & Molecular Biology
Yuan Gao, Xiaochen Gong, Shuang Yu, Zheng Jin, Qicheng Ruan, Chunjing Zhang, Kai Zhao
Summary: The study found that N-2-Hydroxypropyl trimethyl ammonium chloride chitosan/N, O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) showed good immunogenicity and no toxicity as a universal vaccine adjuvant/delivery system. The prepared N-2-HACC/OVA/CMCS NPs induced higher levels of antibodies and immune responses in mice, suggesting their potential in enhancing humoral, cellular, and mucosal immune responses.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Engineering, Environmental
Meiyue Wang, Sen Lin, Mengzhe Liu, Jing Jiao, Hongyan Mi, Jingjing Sun, Yiming Liu, Ruiying Guo, Sirui Liu, Haojie Fu, Yanyu Yang, Rui Li
Summary: By reacting carboxymethyl chitosan (CMC) with dialdehyde-functionalized polyethylene glycol (DF-PEG), an injectable and biocompatible hydrogel was developed. The hydrogel demonstrated wet tissue adhesion, self-healing, and rapid degradation within one week. It acted as a physical barrier to prevent adhesion by covering irregular wounds and fixing to targeted tissues. In a cecum-sidewall abrasion model, the hydrogel significantly reduced the severity of peritoneal adhesion by balancing the extracellular matrix, fibrinolytic system, and inhibiting inflammatory responses. The CMC/DF-PEG hydrogel holds promise as a solution for postoperative antiadhesion in surgery.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Wen Zhang, Shaoqi Yang, Baozeng Ren, Xingmei Lu, Cai Jia
Summary: This study extracted protein from silkworm pupae using functionalized ionic liquids and investigated the relationship between the structure of the liquids and the separation performance of the protein. The results showed that 1-ethyl-3-methylimidazolium chloride could achieve a high extraction yield of protein with low fat content.
CHINESE CHEMICAL LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Mahalakshmi Pandian, Vignesh Selvaprithviraj, Aathira Pradeep, Jayakumar Rangasamy
Summary: The developed N,O-CMC/AgNps hydrogel is adhesive, injectable, conductive, bio-compatible, and exhibits antibacterial and self-healing properties, making it suitable for the treatment of infected wounds.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Food Science & Technology
Yan Dai, Meijia Huang, Yujuan Xu, Lixia Mu, Jingyan Gao, Hongbing Chen, Zhihua Wu, Anshu Yang, Yong Wu, Xin Li
Summary: This study investigated the effect of enzymolysis on the allergenicity of silkworm pupa. The results showed that enzyme hydrolytic technique could significantly reduce the allergenicity of silkworm pupa, and intraperitoneal injection immunization route induced stronger immune response.
FOOD SCIENCE AND HUMAN WELLNESS
(2023)
Review
Biotechnology & Applied Microbiology
Hai-Yan Wang, Yu-Qing Zhang, Zheng-Guo Wei
Summary: Silk fibroin from silkworm Bombyx mori has been extensively researched and applied in various fields in recent decades, including cosmetics, biomedicine, and biomaterials. Dissolution and regeneration of SF fibers is crucial for the application of silk protein-based materials, and the process directly impacts the characteristics and mechanical properties of resulting silk biomaterials. This review summarizes common solvents and dissolution methods for silk protein, properties of resulting SF protein, suitable uses of SF dissolved in corresponding solvents, and recent applications of SF in various biomaterials.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ji-Xin Li, Shu-Xiang Zhao, Yu-Qing Zhang
Summary: This paper describes the use of silk protein for the clean degumming of silk and the preparation of silk protein bioinks for 3D printing of various composite scaffolds. The techniques described here allow for the recycling and utilization of sericin, a waste product of silk processing. The resulting composite scaffolds exhibit improved properties such as enhanced compressive strength and resistance to protease hydrolysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Hai-Yan Wang, Su-Fang Zhou, Meng Zhang, Heng-Da Wang, Yu-Qing Zhang
Summary: Temperature and humidity play crucial roles in controlling the secondary structure and properties of silk fibroin biomaterials. Post-processing under different temperature and humidity conditions can alter the mechanical properties and morphology of silk fibroin films, making them suitable for drug delivery applications.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Biochemistry & Molecular Biology
Zhen-Zhen Wei, Yu-Jie Weng, Yu-Qing Zhang
Summary: Two sericins of high and low molecular weight extracted from commercial silkworm cocoon silk showed significantly enhanced antioxidant and glycosidase inhibitory activities after simulated digestion, with LS performing better than HS.
Article
Biochemistry & Molecular Biology
Zhen-Zhen Wei, Yu-Jie Weng, Yu-Qing Zhang
Summary: The study found that degraded sericin recovered by the Ca(OH)(2) -ultrasound degumming method improved liver injury in T2D rats by activating the damaged PI3K/AKT/AMPK pathway. Oral sericin also reduced inflammatory factors and improved glycogen synthesis, suggesting its potential in functional health foods for lowering blood sugar levels.
Article
Biochemistry & Molecular Biology
Feng-Ya Jing, Yu-Jie Weng, Yu-Qing Zhang
Summary: This study investigated the effects of two types of sericin peptides on AML12 cells in a high-glucose environment, and found that both peptides reduced oxidative stress and inflammation. The low molecular weight peptide also significantly reduced the expression levels of inflammatory factors.
Article
Engineering, Multidisciplinary
Wei Zhen-Zhen, Dong Xuan, Zhang Yu-Qing
Summary: A novel green processing technology was developed to convert egg white into a mechanically robust hydrogel scaffold. The resulting scaffold exhibited high tensile strength, excellent biocompatibility, and a porous structure suitable for tissue engineering applications.
SCIENCE CHINA-TECHNOLOGICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Zhi-Hao Zhong, Yu-Qing Zhang
Summary: Sterile homogeneous egg white (EW) is obtained through high-speed homogenization, centrifugation, and ultraviolet radiation. By incorporating Escherichia coli into the EW and dehydrating it through slow drying, a bacteria-embedded egg white glass (BE-EWG) can be formed. The BE-EWG can maintain cell growth functions and proliferation activities of E. coli for up to one year, and it exhibits a porous hydrogel membrane structure after heat treatment. The method of embedding E. coli cells in EWG as a solid form at room temperature is beneficial for storage, packaging, and shipping at -20 degrees C.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Heng-Da Wang, Yu-Qing Zhang
Summary: Glycated sericin, obtained through Maillard reaction, has increased solubility in water and improved antioxidant capacity. It can be widely applied in functional foods.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Zhi-Hao Zhong, Yu-Qing Zhang
Summary: A novel E. coli cell-immobilizing polyvinyl alcohol hydrogel membrane (ECI-PVAHM) was prepared, which has high water stability, transparency, and mechanical strength, and does not affect the division, proliferation, and metabolic activity of the bacteria. This technology has potential applications in molecular biology and biopharmaceutical production.
MICROBIAL CELL FACTORIES
(2022)
Article
Biochemistry & Molecular Biology
Xuan Dong, Yu-Qing Zhang
Summary: A fragile and water-soluble egg white glass (EWG) was obtained by dehydrating a homogeneous egg white through unidirectional nanopore dehydration (UND). After annealing, the EWG transformed into a water-insoluble, flexible, biocompatible, and mechanically robust egg white hydrogel membrane (EWHM). The combined technology of UND and annealing has potential applications in biomimetic and biomedical materials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Characterization & Testing
Hai-Yan Wang, Heng-Da Wang, Yu-Qing Zhang
Summary: The high molecular weight sericin obtained from degumming method was modified by Maillard reaction with xylose, resulting in water-soluble xylosylated sericin. By combining xylosylated sericin with aqueous PVA solution using unidirectional nanopore dehydration, a mechanically strong and transparent PVA hydrogel composite membrane was obtained. The mechanical properties and enzymatic degradation rate of the composite hydrogel were controlled by UND temperature and xylosylated sericin incorporation.
Article
Chemistry, Multidisciplinary
Ji-Xin Li, Yu-Qing Zhang
Summary: A simple and green method called unidirectional nanopore dehydration (UND) was used to directly process a poly(vinyl alcohol) (PVA) aqueous solution containing egg white (EW) into a composite hydrogel membrane (PVA-EW). The resulting membrane showed good mechanical properties and had a porous networked structure, making it potentially useful in biomaterials, particularly in medical tissue engineering.
Article
Chemistry, Multidisciplinary
Hai-Yan Wang, Shu-Xiang Zhao, Ji-Xin Li, Yu-Qing Zhang
Summary: The study successfully synthesized EW/SF composite scaffolds and found that the scaffolds incorporating 50% G-SF had significantly improved compressive modulus. Compared to the EW scaffold alone, the EW/SF scaffold exhibited higher thermal decomposition temperature and better enzymatic stability. Additionally, the scaffold prolonged the sustained release of insulin and promoted cell adhesion, growth, and proliferation.
Article
Polymer Science
Feng-Ya Jing, Yu-Qing Zhang
Summary: Polyvinyl alcohol (PVA) is a biocompatible and degradable polymer material widely used in the biomedical field. Researchers have developed a novel dehydration method called unidirectional nanopore dehydration (UND) to create a mechanically robust PVA hydrogel membrane (PVAHM) with water stability. The temperature setting during UND affects the mechanical properties of the PVAHM, and higher temperatures result in stronger films. The UND process induces the ordered arrangement of PVA molecular chains and enhances the mechanical properties of the PVAHM.
Article
Materials Science, Biomaterials
Mohammadreza Kasravi, Alireza Yaghoobi, Tahereh Tayebi, Mahsa Hojabri, Abdolkarim Talebi Taheri, Fatemeh Shirzad, Bahram Jambar Nooshin, Radman Mazloomnejad, Armin Ahmadi, Fatemeh A. Tehrani, Ghasem Yazdanpanah, Mohammad Hadi Farjoo, Hassan Niknejad
Summary: As a promising approach in translational medicine, the decellularization of discarded livers to produce bioscaffolds that support recellularization has potential in overcoming the limitations of conventional liver transplantation. In this study, the researchers investigated the use of matrix metalloproteinase (MMP) inhibition to preserve the extracellular matrix (ECM) during liver decellularization. The results demonstrated that the application of an MMP inhibitor significantly improved the preservation of ECM components and mechanical properties of the bioscaffolds, which supported cell viability and function in vitro. The study also confirmed that the MMP inhibition led to the inhibition of MMP2 and MMP9, providing a novel method to enhance ECM preservation during liver decellularization.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Mohammadsadegh Nadimifar, Weiguang Jin, Clara Coll-Satue, Gizem Bor, Paul Joseph Kempen, Ali Akbar Moosavi-Movahedi, Leticia Hosta-Rigau
Summary: This study presents a metal-phenolic self-assembly approach that can prepare nanoparticles fully made of hemoglobin. The nanoparticles exhibit good oxygen binding and releasing capabilities.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jyoti Kumari, Roel Hammink, Jochem Baaij, Frank A. D. T. G. Wagener, Paul H. J. Kouwer
Summary: Fibrosis is the formation of fibrous connective tissue in response to injury, leading to organ dysfunction. A novel hybrid hydrogel combining synthetic polyisocyanide with hyaluronic acid has been developed, showing strong antifibrotic properties.
BIOMATERIALS ADVANCES
(2024)
Letter
Materials Science, Biomaterials
Melissa Machado Rodrigues, Cristian Padilha Fontoura, Charlene Silvestrin Celi Garcia, Sandro Tomaz Martins, Joao Antonio Pegas Henriques, Carlos Alejandro Figueroa, Mariana Roesch Ely, Cesar Aguzzoli
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jessica Polak, David Sachs, Nino Scherrer, Adrian Suess, Huan Liu, Mitchell Levesque, Sabine Werner, Edoardo Mazza, Gaetana Restivo, Mirko Meboldt, Costanza Giampietro
Summary: Human skin equivalents (HSEs) play a crucial role in tissue engineering. This study introduces a 3D-printed culture insert to apply a static radial constraint on HSEs and examines its effects on tissue characteristics. The results show that the diameter of the culture insert significantly influences tissue contraction, fibroblast and matrix organization, keratinocyte differentiation, epidermal stratification, and basement membrane formation. This study provides important insights for the design of skin tissue engineering.
BIOMATERIALS ADVANCES
(2024)
Review
Materials Science, Biomaterials
Shiliang Chen, Tianming Du, Hanbing Zhang, Jing Qi, Yanping Zhang, Yongliang Mu, Aike Qiao
Summary: This paper reviewed the primary methods for improving the overall properties of biodegradable zinc stents. It discussed the mechanical properties, degradation behavior, and biocompatibility of various improvement strategies. Alloying was found to be the most common, simple, and effective method for improving mechanical properties. Deformation processing and surface modification further improved the mechanical properties and biological activity of zinc alloys. Meanwhile, structural design could endow stents with special properties. Manufacturing zinc alloys with excellent properties and exploring their interaction mechanism with the human body are areas for future research.
BIOMATERIALS ADVANCES
(2024)