Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 59, Issue -, Pages 1038-1046Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2015.11.007
Keywords
Collagen; Ultrasonic treatment; Fibril formation; Collagen gels; Properties
Categories
Funding
- National Natural Science Foundation of China [21376183]
- Application and Foundation Study of Wuhan, China [2013020501010177]
- Innovation Team Program of Hubei province, China [T201208]
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Controlling the fibril-formation process of collagen in vitro to fabricate novel biomaterials is a new area in the field of collagen research. This study aimed to determine the effect of ultrasonication on collagen fibril formation and the properties of the resulting collagen gels. Native collagen, extracted from the skin of grass carp, self-assembled under ultrasonic conditions (at different ultrasonic power and duration). The self-assembly kinetics, fibrillar morphology, and physical and cell growth-promoting properties of the collagen gels were analyzed and compared. The results showed that the self-assembly rate of collagen was increased by ultrasonication at the nucleation stage. The resulting fibrils exhibited smaller diameters and D-periodicity lengths than that of the untreated collagen samples (p < 0.05). The viscoelasticity and textural properties of collagen gels also changed after ultrasonication at the nucleation stage. Texture profile analysis and cell proliferation assays showed that ultrasonication produced softer collagen gel colloids, which were more suitable for cell proliferation than the untreated collagen gels. (C) 2015 Elsevier B.V. All rights reserved.
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