Article
Biochemical Research Methods
Luyan Wang, Kaili Cui, Pengxiang Wang, Meishan Pei, Wenjuan Guo
Summary: A novel electrochemical DNA sensor was developed using accordion-like Ti3C2Tx to detect Helicobacter pylori, with a large specific surface area and excellent conductivity. The modified electrode supported by Au nanoparticles effectively fixed the capture probe, allowing for signal amplification during detection and enhancing sensitivity. The sandwich structure formed by hybridizing cpDNA with target DNA sequence and rpDNA greatly increased the current signal and improved the performance of the sensor.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Kirti Saxena, Arun Kumar, Nidhi Chauhan, Manika Khanuja, Bansi D. Malhotra, Utkarsh Jain
Summary: An electrochemical biosensor was designed for detecting a specific toxin of Helicobacter pylori, utilizing a nanocomposite-modified gold electrode and VacA antibodies for detection. The sensor showed good stability, sensitivity, and selectivity.
Article
Chemistry, Analytical
Xiaojie Song, Man-Man Lv, Qi-Yan Lv, Hui-Fang Cui, Jing Fu, Yu-Yang Huo
Summary: A novel and sensitive Helicobacter pylori assay strategy was developed using LIAR and DNAzymes technology, utilizing specific tDNA primer and Md-Dz amplicon for detection. The assay showed high efficiency, sensitivity, and selectivity, with a wide linear detection range and low LOD. The DNA sensor is stable and universally applicable for detecting various biological samples and short DNA sequences.
MICROCHEMICAL JOURNAL
(2021)
Article
Electrochemistry
Fernando Otero, Kim Shortall, Urszula Salaj-Kosla, Syed A. M. Tofail, Edmond Magner
Summary: An electrochemical biosensor was developed using a hairpin DNA probe labeled with methylene blue for detecting the TP53 gene. The biosensor could selectively detect a single nucleotide polymorphism in the TP53 gene affecting residue 175 of the p53 protein, with a more pronounced response at shorter hybridization times.
ELECTROCHIMICA ACTA
(2021)
Article
Biophysics
Yuuki Inoue, Yeji Kim, Hijiri Hasegawa, Yasukazu Yoshida, Keita Sakakibara, Yoshinobu Tsujii
Summary: We developed a novel electrochemical biosensor electrode by constructing an original conductive substrate modified with a double-layered polymer brush structure. The experimental results showed that the gold electrode with a double-layered polymer brush structure and carboxyl groups exhibited a linear correlation with potential difference and hydrogen ion concentration. This is the first application of the double-layered polymer brush structure in electrochemical biosensing and it is expected to be an excellent surface modification method for reducing background noise.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Ji Yeong Yang, Jong-Bae Kim, Pyeongjae Lee, Sa-Hyun Kim
Summary: Evodiamine inhibits the growth of H. pylori by downregulating gene expressions related to replication and transcription machineries of the bacteria. Moreover, evodiamine also suppresses urease expression, leading to reduced translocation of CagA and VacA proteins into AGS cells. Additionally, evodiamine inhibits the activation of signaling proteins induced by H. pylori infection, contributing to reduction of IL-8 production in AGS cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Applied
Yan Guan, Peng-Bin Si, Tong Yang, Yuan Wu, Yun-Hui Yang, Rong Hu
Summary: A DNA walker-based dual-signal electrochemical ratiometric platform was developed for OTA detection, using Co-MOFs and toluidine blue as signal tag and reference probe. The method displayed superior sensitivity and reproducibility, with comparable results to commercial enzyme-linked immunoassay kits in determining OTA content in red wine samples.
Article
Chemistry, Analytical
Yawen He, Fei Jia, Yuxin Sun, Weihuan Fang, Yanbin Li, Juhong Chen, Yingchun Fu
Summary: In this study, an electrochemical sensing method was developed to detect S. Typhimurium using PCR and CRISPR / Cas12a technology. The method allows sensitive detection of the pathogen in pure cultures and spiked poultry meat.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemical Research Methods
Kirti Saxena, Nidhi Chauhan, Utkarsh Jain
Summary: This review introduces various biosensors for H. pylori detection, discussing their components, biorecognition elements, transducers, and biomarkers associated with H. pylori pathogenesis. It also summarizes the prospects of advancement and commercialization of point-of-care tools.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Chemistry, Analytical
Linfei Xiong, Zhaohui Li, Guangming Li, Huangxian Ju
Summary: This study developed a DNA dendrimer for loading signal molecules and constructing an amplified electrochemical immunosensing method. The DNA dendrimer was self-assembled by hybridizing complementary oligonucleotides, enabling signal amplification. The method showed excellent performance in analyzing prostate specific antigen as a model analyte.
ANALYTICA CHIMICA ACTA
(2021)
Review
Chemistry, Analytical
Yong Chang, Yanyan Wang, Jingyi Zhang, Yuejiao Xing, Gang Li, Dehua Deng, Lin Liu
Summary: Magnetically assisted biosensors have important applications in electrochemical biosensors, enabling separation and pre-concentration of targets through the combination of magnetic materials and biomolecules, and obtaining signals through measurements such as redox current.
Review
Biotechnology & Applied Microbiology
Linqi Zhu, Yue Huang, Hong Li, Shihe Shao
Summary: Infection with Helicobacter pylori may promote the occurrence and progression of gastric cancer by influencing the tumor microenvironment (TME).
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biophysics
Ssu-Chia Chen, Kuan-Ting Chen, Amily Fang-Ju Jou
Summary: The novel dual-amplification-boosted electrochemical biosensor was developed for detecting pancreatic cancer-associated miR-196b, showing a detection limit of 0.26 pM and nice analytical reproducibility. The biosensor offers great promise as a complementary tool for real sample detection in the clinic.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Biochemistry & Molecular Biology
Rosanna Capparelli, Domenico Iannelli
Summary: Epigenetics plays a role in regulating gene expression, cell differentiation, and response to environmental stimuli. Helicobacter pylori (Hp) uses DNA methylation to silence genes in human gastric mucosa, which is a primary cause of gastric cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Majid Arvand, Hoda Ilkhani, Mohamad Reza Ganjali, Akram Pourhabib
Summary: A new Tb complex Tb(QS)3 was used to investigate the interaction between the complex and double-stranded DNA, leading to the design of a new DNA biosensor. The interaction mechanism, primarily intercalative interaction, was elucidated using electrochemical methods such as cyclic voltammetry and differential pulse voltammetry.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Microbiology
Elham Kazemi, Vahideh Tarhriz, Mohammad Ali Amoozegar, Mohammad Saeid Hejazi
Summary: A novel slightly halophilic bacterium, Halomonas azerbaijanica, was isolated from Urmia Lake in Iran. It thrives in high salinity, moderate temperatures, and neutral pH conditions, belonging to the genus Halomonas.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Cell & Tissue Engineering
Parisa Nikasa, Tine Tricot, Nejat Mahdieh, Hossein Baharvand, Mehdi Totonchi, Mohammad Saeid Hejazi, Catherine M. Verfaillie
Summary: Autosomal recessive hypercholesterolemia (ARH) is a rare monogenic disorder caused by pathogenic variants in the LDLRAP1 gene, leading to variable responses to treatment. Patient-specific iPSC-derived hepatocyte-like cells have been validated as an appropriate tool to model ARH disease, providing insights into LDLRAP1 mutations and protein function.
STEM CELLS AND DEVELOPMENT
(2021)
Review
Immunology
Mohammad Ali Zolfaghari, Reza Arefnezhad, Forough Parhizkar, Mohammad Saeid Hejazi, Farhad Motavalli Khiavi, Ata Mahmoodpoor, Mehdi Yousefi
Summary: This review explores the dysregulation of cytokines produced from T cells, the imbalance of transcription factors of Th cells, the expression of chemokine receptors on T cells, as well as the dysregulation of various miRNAs related to T cells, which may contribute to the development of preeclampsia.
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2021)
Article
Microbiology
Ruan Wenting, Soheila Montazersaheb, Shehzad Abid Khan, Hyung Min Kim, Vahideh Tarhriz, Mohammad Amin Hejazi, Mohammad Saeid Hejazi, Che Ok Jeon
Summary: TBZ9(T) is a novel moderately halophilic bacterium isolated from Urmia Lake in Iran, belonging to the genus Halomonas. It shows optimal growth at 3% salt concentration, 30 degrees Celsius temperature, and pH 7.0.
CURRENT MICROBIOLOGY
(2021)
Article
Immunology
Mohammad Ali Zolfaghari, Roza Motavalli, Mohammad Sadegh Soltani-Zangbar, Forough Parhizkar, Shahla Danaii, Leili Aghebati-Maleki, Mohammad Noori, Sanam Dolati, Majid Ahmadi, Hossein Samadi Kafil, Farhad Jadidi-Niaragh, Javad Ahmadian Heris, Ata Mahmoodpoor, Mohammad Saeid Hejazi, Mehdi Yousefi
Summary: The study revealed that in patients with preeclampsia, the expression level of miRNA-326 in CD4(+) cells increased, leading to the inhibition of Ets-1, a negative regulator of Th17 cell development. Additionally, the number and expression level of Th17 cells and transcription factor ROR gamma t increased, while Treg cells and transcription factor Foxp3 showed a decrease. Flow cytometry analysis indicated an increase in the Th17/Treg ratio, along with heightened expression levels of cytokines and anti-angiogenic molecules in isolated CD4(+) cells from preeclampsia patients.
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2021)
Review
Cardiac & Cardiovascular Systems
Leila Abkhooie, Mostafa M. Sarabi, Houman Kahroba, Shirin Eyvazi, Soheila Montazersaheb, Vahideh Tarhriz, Mohammad S. Hejazi
Summary: MyomiRs, known as crucial regulatory elements for cardiovascular development, are abundantly expressed in the myocardium and have been confirmed as promising diagnostic biomarkers for early diagnosis of cardiac disorders. Recent findings have focused on epigenetic modulations of MyomiRs and cardiac regeneration associated with cardiac diseases.
CURRENT CARDIOLOGY REVIEWS
(2021)
Article
Chemistry, Multidisciplinary
Rokhsareh Abedi, Jahan Bakhsh Raoof, Ayemeh Bagheri Hashkavayi, Maryam Asghari, Razieh Azimi, Mohammad Saeid Hejazi
Summary: An electrochemical DNA hybridization biosensor based on modified graphite screen-printed electrodes was developed for detection of short-sequence DNA of prostate cancer gene. The sensor demonstrated high selectivity and sensitivity in detecting the hybridization process through differential pulse voltammetric signals.
JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
(2022)
Article
Pharmacology & Pharmacy
Houman Kahroba, Nasser Samadi, Mostafa Mostafazadeh, Mohamad Saied Hejazi, Mohammad Reza Sadeghi, Shahryar Hashemzadeh, Amir Taher Eftekhar Sadat, Abbas Karimi
Summary: The study identified four miRNAs in serum exosomes of gastric cancer patients that are directly associated with tumoral miRNA expression profile, with diagnostic potential. These miRNAs were significantly upregulated in exosomes and showed high correlation in expression between individuals.
Review
Virology
Soheila Montazersaheb, Seyed Mahdi Hosseiniyan Khatibi, Mohammad Saeid Hejazi, Vahideh Tarhriz, Afsaneh Farjami, Faramarz Ghasemian Sorbeni, Raheleh Farahzadi, Tohid Ghasemnejad
Summary: COVID-19 caused by SARS-CoV-2 poses a significant global health threat, leading to pneumonia and ARDS in patients. Cytokine storm, characterized by elevated levels of pro-inflammatory cytokines, including interleukins and TNF-alpha, contributes to lung damage in ARDS patients. Suppressing the inflammatory response may be a possible strategy for managing COVID-19.
Article
Biology
M. Omidi-Shahsavandi, P. Yaghmaei, S. Ahmadian, A. Ebrahim-Habibi
Summary: Silibinin and trans-chalcone were found to have inhibitory effects on insulin amyloid formation, with Silibinin showing a better effect. Animal experiments also showed that these two compounds can reduce amyloid plaque formation and improve cognitive function. Molecular docking experiments provided insights into the binding site of these compounds with insulin.
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
(2023)
Review
Pharmacology & Pharmacy
Jeiran Pashapour-Anousheh, Abolfazl Barzegar, Mohammad Saeid Hejazi, Nikoo Hasheminejad, Hamid Reza Heidari, Elnaz Mehdizadeh Aghdam
Summary: This article reviews the mechanism and strategies for inhibiting Ras-mutated cancers. Currently, there are no approved Ras mutant inhibitors for clinical use. Research needs to focus on inhibiting specific mutants without affecting wild-type Ras variants.
PHARMACEUTICAL SCIENCES
(2022)
Article
Medicine, General & Internal
Leila Abkhooie, Mostafa Moradi Sarabi, Houman Kahroba, Hossein Ghanbarian, Soheila Montazer Saheb, Vahideh Tarhriz, Mohammad Saeid Hejazi
Summary: The study demonstrates that Cdk9 overexpression can induce DNA methylation changes in mouse myoblast cells, and this overexpression also increases the expression levels of DNMTs, further activating global and regional DNA methylation profiles.
CRESCENT JOURNAL OF MEDICAL AND BIOLOGICAL SCIENCES
(2022)
Article
Endocrinology & Metabolism
Parisa Nikasa, Bahareh Rabbani, Mohammad Saeid Hejazi, Ata Firouzi, Hossein Baharvand, Mehdi Totonchi, Nejat Mahdieh
CLINICAL PEDIATRIC ENDOCRINOLOGY
(2021)
Article
Pharmacology & Pharmacy
Vida Ebrahimi, Shirin Eyvazi, Soheila Montazersaheb, Parivar Yazdani, Mohammad Amin Hejazi, Vahideh Tarhriz, Mohammad Saeid Hejazi
Summary: Aquatic biodegrading bacteria capable of using PAHs as the sole carbon source were isolated from the Caspian lake, with 11 out of 23 bacterial isolates showing growth on supplemented media and displaying high degradation potential for phenanthrene and naphthalene. These bacteria play a critical role in the biodegradation of PAHs in the lake and contribute to the purification process of the habitat.
PHARMACEUTICAL SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Faegheh Golabi, Elnaz Mehdizadeh Aghdam, Mousa Shamsi, Mohammad Hosein Sedaaghi, Abolfazl Barzegar, Mohammad Saeid Hejazi
Summary: The study applied BLBFE strategy and other feature extraction methods for the classification of riboswitch sequences, demonstrating higher accuracy, sensitivity, specificity, and f-score values in performance evaluation.
Article
Materials Science, Biomaterials
Mohammadreza Kasravi, Alireza Yaghoobi, Tahereh Tayebi, Mahsa Hojabri, Abdolkarim Talebi Taheri, Fatemeh Shirzad, Bahram Jambar Nooshin, Radman Mazloomnejad, Armin Ahmadi, Fatemeh A. Tehrani, Ghasem Yazdanpanah, Mohammad Hadi Farjoo, Hassan Niknejad
Summary: As a promising approach in translational medicine, the decellularization of discarded livers to produce bioscaffolds that support recellularization has potential in overcoming the limitations of conventional liver transplantation. In this study, the researchers investigated the use of matrix metalloproteinase (MMP) inhibition to preserve the extracellular matrix (ECM) during liver decellularization. The results demonstrated that the application of an MMP inhibitor significantly improved the preservation of ECM components and mechanical properties of the bioscaffolds, which supported cell viability and function in vitro. The study also confirmed that the MMP inhibition led to the inhibition of MMP2 and MMP9, providing a novel method to enhance ECM preservation during liver decellularization.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Mohammadsadegh Nadimifar, Weiguang Jin, Clara Coll-Satue, Gizem Bor, Paul Joseph Kempen, Ali Akbar Moosavi-Movahedi, Leticia Hosta-Rigau
Summary: This study presents a metal-phenolic self-assembly approach that can prepare nanoparticles fully made of hemoglobin. The nanoparticles exhibit good oxygen binding and releasing capabilities.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jyoti Kumari, Roel Hammink, Jochem Baaij, Frank A. D. T. G. Wagener, Paul H. J. Kouwer
Summary: Fibrosis is the formation of fibrous connective tissue in response to injury, leading to organ dysfunction. A novel hybrid hydrogel combining synthetic polyisocyanide with hyaluronic acid has been developed, showing strong antifibrotic properties.
BIOMATERIALS ADVANCES
(2024)
Letter
Materials Science, Biomaterials
Melissa Machado Rodrigues, Cristian Padilha Fontoura, Charlene Silvestrin Celi Garcia, Sandro Tomaz Martins, Joao Antonio Pegas Henriques, Carlos Alejandro Figueroa, Mariana Roesch Ely, Cesar Aguzzoli
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jessica Polak, David Sachs, Nino Scherrer, Adrian Suess, Huan Liu, Mitchell Levesque, Sabine Werner, Edoardo Mazza, Gaetana Restivo, Mirko Meboldt, Costanza Giampietro
Summary: Human skin equivalents (HSEs) play a crucial role in tissue engineering. This study introduces a 3D-printed culture insert to apply a static radial constraint on HSEs and examines its effects on tissue characteristics. The results show that the diameter of the culture insert significantly influences tissue contraction, fibroblast and matrix organization, keratinocyte differentiation, epidermal stratification, and basement membrane formation. This study provides important insights for the design of skin tissue engineering.
BIOMATERIALS ADVANCES
(2024)
Review
Materials Science, Biomaterials
Shiliang Chen, Tianming Du, Hanbing Zhang, Jing Qi, Yanping Zhang, Yongliang Mu, Aike Qiao
Summary: This paper reviewed the primary methods for improving the overall properties of biodegradable zinc stents. It discussed the mechanical properties, degradation behavior, and biocompatibility of various improvement strategies. Alloying was found to be the most common, simple, and effective method for improving mechanical properties. Deformation processing and surface modification further improved the mechanical properties and biological activity of zinc alloys. Meanwhile, structural design could endow stents with special properties. Manufacturing zinc alloys with excellent properties and exploring their interaction mechanism with the human body are areas for future research.
BIOMATERIALS ADVANCES
(2024)
