Review
Biochemistry & Molecular Biology
Priti Gupta, Shilpa Sharma, Shagufta Jabin, Sapana Jadoun
Summary: Regenerative medicine and tissue engineering have great potential in addressing the limitations of traditional medical approaches. Chitosan, as a naturally derived biocompatible biomaterial, has played a significant role in tissue regeneration. It has unique properties and can be applied in various fields, such as agriculture, nutraceuticals, biomedicine, and food. Chitosan is also an important material for developing next-generation hydrogels and bio-scaffolds. Furthermore, chitosan can be easily modified to enhance its mechanical strength, biodegradability, and controlled release of bioactive molecules. Blending chitosan with other polymers or incorporating nanoparticles expands its possibilities in tissue engineering applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Engineering, Biomedical
Qi Feng, Dingguo Li, Qingtao Li, Xiaodong Cao, Hua Dong
Summary: This mini-review summarizes the fabrication strategies, unique characteristics, and applications of microgel assembly in tissue engineering and regenerative medicine, with a focus on 3D printing. The challenges for further applications of microgel assembly are also discussed.
BIOACTIVE MATERIALS
(2022)
Review
Pharmacology & Pharmacy
Izeia Lukin, Itsasne Erezuma, Lidia Maeso, Jon Zarate, Martin Federico Desimone, Taleb H. Al-Tel, Alireza Dolatshahi-Pirouz, Gorka Orive
Summary: Tissue engineering has become a viable medical alternative in response to the increasing lifespan of society. This review focuses on gelatin, a widely used biomaterial in tissue engineering, and discusses ongoing concerns and the latest developments in this field. Advancements in technology, such as 3D bioprinting and gelatin functionalization, have allowed for better replication of native tissue in gelatin-based systems. This breakthrough has implications for both the manufacturing process, making it more practical and cost-effective, and the future use of gelatin in disease detection and diagnosis rather than treatment.
Review
Biochemistry & Molecular Biology
Nikita Voloshin, Pyotr Tyurin-Kuzmin, Maxim Karagyaur, Zhanna Akopyan, Konstantin Kulebyakin
Summary: Immortalized cells are increasingly used in practical medicine due to their advantages, such as the ability to produce larger amounts of cells and use them for longer periods of time. However, they also have drawbacks, like the possibility of malignant transformation or major phenotype change. This review discusses ways to overcome these barriers and highlights specific fields of application where these concerns are minimal.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Sarah M. Hull, Lucia G. Brunel, Sarah C. Heilshorn
Summary: Using gel-phase materials as bioinks offers advantages in providing cell protection and biological signals. Challenges remain in terms of cell compatibility and biological functionality of bioink materials.
ADVANCED MATERIALS
(2022)
Review
Chemistry, Multidisciplinary
Lin Bao, Xuejing Cui, Monika Mortimer, Xiaoyu Wang, Junguang Wu, Chunying Chen
Summary: The severe imbalance between supply and demand in organ resection or severe tissue damage poses life-threatening risks. Carbon nanotubes (CNTs) have emerged as promising platforms due to their unique properties. CNTs are commonly used as additives in tissue engineering scaffolds to modify structure, porosity, mechanical strength, and more. This review summarizes the applications and design strategies of CNTs in various tissue engineering fields and discusses their potential biosafety risks and solutions. Future developments of CNTs in tissue engineering are also speculated.
Review
Materials Science, Multidisciplinary
Shivesh Anand, Serena Danti, Lorenzo Moroni, Carlos Mota
Summary: It is estimated that by 2050 one in every ten people will be suffering from disabling hearing loss. Perforated tympanic membranes (TMs) are the most common injury to the human ear, resulting in a partial or complete hearing loss due to inept sound conduction. Tissue engineering approaches, including TM repair and reconstruction, have been developed for TM regeneration.
PROGRESS IN MATERIALS SCIENCE
(2022)
Review
Engineering, Biomedical
Michelle Koerselman, Lisanne C. M. Morshuis, Marcel Karperien
Summary: This article discusses the application of small molecules such as peptides, aptamers, and variable antigen-binding domain of heavy chain only antibodies in tissue engineering and regenerative medicine. It compares these biological functionalities with conventional antibodies and provides an overview of recent work in this area. The article focuses on the structural differences, modifications, and conjugation strategies of these biologicals, as well as the clinical trials involving them. The challenges associated with each of these biologicals are also highlighted.
ACTA BIOMATERIALIA
(2023)
Review
Engineering, Biomedical
Daniel Salthouse, Katarina Novakovic, Catharien M. U. Hilkens, Ana Marina Ferreira
Summary: This review highlights the importance of the immune response to biomaterials and discusses challenges and opportunities in the development and standardized assessment of the immune response to biomaterials, including the importance of endotoxin levels. It also explores the properties of biomaterials that impact the host immune response and the use of immunomodulatory biomaterials in regenerative medicine. Finally, a standardized in vitro pathway for evaluating the immune response to biomaterials (hydrogels) and their regenerative potential is proposed to ensure safety, consistency, and reduce costs and animal use.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Physical
Ramon Rial, Natalia Hassan, Zhen Liu, Juan M. Ruso
Summary: The study successfully produced hydrogel microparticles with different morphologies and compositions by combining experimental and computational methods, incorporating drug-doped Hydroxyapatite in their inner matrix. The use of a microfluidic system to obtain crosslinked HMPs with homogeneous sizes and morphologies, as well as the adsorption kinetics and desorption profiles of the chosen drug models, were key aspects of the research. The development of these hydrogel microparticles with improved functionalities is crucial for innovative and sustainable solutions in regenerative medicine.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Chemistry, Multidisciplinary
Koichiro Hayashi, Toshiki Yanagisawa, Ryo Kishida, Kunio Ishikawa
Summary: This study investigates the effects of scaffold shape on tissue generation. The results show that even tiny protuberances on the scaffold surface can affect the percentage of interscaffold space, thereby exerting dominant effects on osteogenesis.
Review
Engineering, Biomedical
Yiting Lou, Huiming Wang, Guanchen Ye, Yongzheng Li, Chao Liu, Mengfei Yu, Binbin Ying
Summary: This review discusses the important role of periosteum in bone defect repair and regeneration, as well as the need for developing tissue-engineered periosteum (TEP). The construction strategies for TEP are explored from the perspectives of materials, mechanical properties, cell-based strategies, and combination with growth factors. Current challenges and future perspectives for TEP development are also discussed.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Chemistry, Multidisciplinary
Zhen Wang, Wasim Kapadia, Cuidi Li, Feng Lin, Ruben F. Pereira, Pedro L. Granja, Bruno Sarmento, Wenguo Cui
Summary: The human body is composed of four basic tissue types: epithelial, connective, muscular, and nervous tissues, each consisting of cells with similar functions. Three-dimensional bioprinting, based on histological categories, is a versatile technology that can form functional organ structures simply. This technology shows remarkable regenerative power and novel breakthroughs in specific tissues like muscle and nerve tissues.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Multidisciplinary Sciences
David R. Maestas, Liam Chung, Jin Han, Xiaokun Wang, Sven D. Sommerfeld, Sean H. Kelly, Erika Moore, Helen Hieu Nguyen, Joscelyn C. Mejias, Alexis N. Pena, Hong Zhang, Joshua S. T. Hooks, Alexander F. Chin, James I. Andorko, Cynthia A. Berlinicke, Kavita Krishnan, Younghwan Choi, Amy E. Anderson, Ronak Mahatme, Christopher Mejia, Marie Eric, JiWon Woo, Sudipto Ganguly, Donald J. Zack, Liang Zhao, Edward J. Pearce, Franck Housseau, Drew M. Pardoll, Jennifer H. Elisseeff
Summary: The immune system plays an important role in tissue repair. A regenerative immunotherapy using the helminth Schistosoma mansoni soluble egg antigen (SEA) was developed, which stimulates the production of IL-4 and other type 2-associated cytokines. The application of regenerative SEA (rSEA) promotes accumulation of IL-4-expressing T helper cells, eosinophils, and regulatory T cells, leading to improved repair and reduced fibrosis. Encapsulation and controlled release of rSEA in a hydrogel further enhance tissue repair.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biophysics
M. R. Poorna, R. Jayakumar, Jyh-Ping Chen, Ullas Mony
Summary: Induced pluripotent stem cells (iPSCs) have great potential in regenerative medicine, and improving culture methods, such as using hydrogels as substrates, can support iPSC adhesion, proliferation, and differentiation. Using hydrogels may help generate large numbers of clinical-grade cells for potential clinical applications.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Review
Chemistry, Medicinal
Giuseppina Sabatino, Ivan Guryanov, Andrea Rombecchi, Jacopo Zanon, Antonio Ricci, Walter Cabri, Anna Maria Papini, Paolo Rovero
EXPERT OPINION ON THERAPEUTIC PATENTS
(2016)
Article
Nanoscience & Nanotechnology
Ivan Guryanov, Sabrina Cipriani, Stefano Fiorucci, Natalia Zashikhina, Silvia Marchiano, Paolo Scarpelli, Viktor Korzhikov-Vlakh, Ekaterina Popova, Evgenia Korzhikova-Vlakh, Barbara Biondi, Fernando Formaggio, Tatiana Tennikova
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2017)
Article
Chemistry, Multidisciplinary
Ivan Guryanov, Federico Polo, Evgeniy V. Ubyvovk, Evgenia Korzhikova-Vlakh, Tatiana Tennikova, Armin T. Rad, Mu-Ping Nieh, Flavio Maran
Article
Polymer Science
Viktor Korzhikov-Vlakh, Ilia Averianov, Ekaterina Sinitsyna, Yuliya Nashchekina, Dmitry Polyakov, Ivan Guryanov, Antonina Lavrentieva, Lukas Raddatz, Evgenia Korzhikova-Vlakh, Thomas Scheper, Tatiana Tennikova
Article
Biochemistry & Molecular Biology
I. Guryanov, F. Real-Fernandez, G. Sabatino, N. Prisco, V. Korzhikov-Vlakh, B. Biondi, A. M. Papini, E. Korzhikova-Vlakh, P. Rovero, T. Tennikova
JOURNAL OF PEPTIDE SCIENCE
(2019)
Article
Chemistry, Applied
Ivan Guryanov, Andrea Orlandin, Angelo Viola, Barbara Biondi, Denis Badocco, Fernando Formaggio, Antonio Ricci, Walter Cabri
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2019)
Article
Chemistry, Applied
Ivan Guryanov, Andrea Orlandin, Angelo Viola, Barbara Biondi, Fernando Formaggio, Antonio Ricci, Walter Cabri
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2020)
Article
Polymer Science
Viktor Korzhikov-Vlakh, Iuliia Katernuk, Iuliia Pilipenko, Antonina Lavrentieva, Ivan Guryanov, Vladimir Sharoyko, Alina A. Manshina, Tatiana B. Tennikova
Article
Chemistry, Medicinal
Ivan Guryanov, Viktor Korzhikov-Vlakh, Madhushree Bhattacharya, Barbara Biondi, Giulia Masiero, Fernando Formaggio, Tatiana Tennikova, Arto Urtti
Summary: The study focused on the design of VEGF inhibitors by introducing a conformational constrain in peptides with the non-proteinogenic amino acid Aib. The insertion of Aib significantly enhanced the peptide's affinity to VEGF and improved stability in the vitreous environment. These Aib-containing peptides show promise as candidates for designing VEGF inhibitors with enhanced properties.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Ivan Guryanov, Andrea Orlandin, Ivan De Paola, Angelo Viola, Barbara Biondi, Denis Badocco, Fernando Formaggio, Antonio Ricci, Walter Cabri
Summary: Peptides as APIs are gaining interest, requiring efficient preparation strategies. This study presents a method for the synthesis of GLP-1 receptor agonist lipopeptides using pseudoproline-assisted convergent synthesis, effectively preventing peptide aggregation and improving yield. A novel approach involving copper(II) lysinate intermediates is also developed for the synthesis of lipidated side chains, which is suitable for industrial preparation of similar branched peptides.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2021)
Article
Polymer Science
Mariia Stepanova, Anatoliy Dobrodumov, Ilia Averianov, Iosif Gofman, Juliya Nashchekina, Ivan Guryanov, Ilya Klyukin, Andrey Zhdanov, Evgenia Korzhikova-Vlakh, Konstantin Zhizhin
Summary: Boron neutron capture therapy (BNCT) is a promising cancer treatment approach. In the case of osteosarcoma, boron-containing scaffolds can deliver boron to tumor cells and repair bone defects. This study fabricated and characterized biodegradable polymer composites based on aliphatic polyesters containing closo-borates (CB) for BNCT. The mechanical properties, degradation kinetics, and release of closo-borate were investigated. The results confirmed the potential of these composites for BNCT and bone tissue regeneration.
Article
Pharmacology & Pharmacy
Lucia Ferrazzano, Alessandra Tolomelli, Ivan Guryanov, Marco Macis, Ulrich Abel, Antonio Ricci, Walter Cabri
Summary: One of the main objectives of peptide drug design is to improve peptide pharmacokinetics while maintaining biological activity through complex modifications of the peptides' primary structure. However, these changes often lead to the formation of peculiar impurities in the peptide drugs and their metabolites, which require advanced analytical methods to properly assess their content.
PHARMACOLOGY RESEARCH & PERSPECTIVES
(2023)
Review
Pharmacology & Pharmacy
Ivan Guryanov, Tatiana Tennikova, Arto Urtti
Summary: VEGFs play crucial roles in angiogenesis, but dysregulation can lead to pathological angiogenesis in various diseases. Monoclonal antibodies and decoy receptors are commonly used to neutralize VEGFA in anti-angiogenic therapies. Short peptides are promising alternatives to full-length VEGFA inhibitors, overcoming some limitations of existing therapies.
Meeting Abstract
Biochemistry & Molecular Biology
Marta De Zotti, Ivan Guryanov, Tatiana Tennikova, Madhushree Bhattacharya, Arto Urtti
JOURNAL OF PEPTIDE SCIENCE
(2018)
Article
Chemistry, Multidisciplinary
Irina Tarasenko, Natalia Zashikhina, Ivan Guryanov, Maria Volokitina, Barbara Biondi, Stefano Fiorucci, Fernando Formaggio, Tatiana Tennikova, Evgenia Korzhikova-Vlakh
Article
Materials Science, Biomaterials
Mohammadreza Kasravi, Alireza Yaghoobi, Tahereh Tayebi, Mahsa Hojabri, Abdolkarim Talebi Taheri, Fatemeh Shirzad, Bahram Jambar Nooshin, Radman Mazloomnejad, Armin Ahmadi, Fatemeh A. Tehrani, Ghasem Yazdanpanah, Mohammad Hadi Farjoo, Hassan Niknejad
Summary: As a promising approach in translational medicine, the decellularization of discarded livers to produce bioscaffolds that support recellularization has potential in overcoming the limitations of conventional liver transplantation. In this study, the researchers investigated the use of matrix metalloproteinase (MMP) inhibition to preserve the extracellular matrix (ECM) during liver decellularization. The results demonstrated that the application of an MMP inhibitor significantly improved the preservation of ECM components and mechanical properties of the bioscaffolds, which supported cell viability and function in vitro. The study also confirmed that the MMP inhibition led to the inhibition of MMP2 and MMP9, providing a novel method to enhance ECM preservation during liver decellularization.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Mohammadsadegh Nadimifar, Weiguang Jin, Clara Coll-Satue, Gizem Bor, Paul Joseph Kempen, Ali Akbar Moosavi-Movahedi, Leticia Hosta-Rigau
Summary: This study presents a metal-phenolic self-assembly approach that can prepare nanoparticles fully made of hemoglobin. The nanoparticles exhibit good oxygen binding and releasing capabilities.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jyoti Kumari, Roel Hammink, Jochem Baaij, Frank A. D. T. G. Wagener, Paul H. J. Kouwer
Summary: Fibrosis is the formation of fibrous connective tissue in response to injury, leading to organ dysfunction. A novel hybrid hydrogel combining synthetic polyisocyanide with hyaluronic acid has been developed, showing strong antifibrotic properties.
BIOMATERIALS ADVANCES
(2024)
Letter
Materials Science, Biomaterials
Melissa Machado Rodrigues, Cristian Padilha Fontoura, Charlene Silvestrin Celi Garcia, Sandro Tomaz Martins, Joao Antonio Pegas Henriques, Carlos Alejandro Figueroa, Mariana Roesch Ely, Cesar Aguzzoli
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jessica Polak, David Sachs, Nino Scherrer, Adrian Suess, Huan Liu, Mitchell Levesque, Sabine Werner, Edoardo Mazza, Gaetana Restivo, Mirko Meboldt, Costanza Giampietro
Summary: Human skin equivalents (HSEs) play a crucial role in tissue engineering. This study introduces a 3D-printed culture insert to apply a static radial constraint on HSEs and examines its effects on tissue characteristics. The results show that the diameter of the culture insert significantly influences tissue contraction, fibroblast and matrix organization, keratinocyte differentiation, epidermal stratification, and basement membrane formation. This study provides important insights for the design of skin tissue engineering.
BIOMATERIALS ADVANCES
(2024)
Review
Materials Science, Biomaterials
Shiliang Chen, Tianming Du, Hanbing Zhang, Jing Qi, Yanping Zhang, Yongliang Mu, Aike Qiao
Summary: This paper reviewed the primary methods for improving the overall properties of biodegradable zinc stents. It discussed the mechanical properties, degradation behavior, and biocompatibility of various improvement strategies. Alloying was found to be the most common, simple, and effective method for improving mechanical properties. Deformation processing and surface modification further improved the mechanical properties and biological activity of zinc alloys. Meanwhile, structural design could endow stents with special properties. Manufacturing zinc alloys with excellent properties and exploring their interaction mechanism with the human body are areas for future research.
BIOMATERIALS ADVANCES
(2024)
