Full validation of therapeutic antibody sequences by middle-up mass measurements and middle-down protein sequencing

Antibodies to watch in 2016

(2015) Janice M. Reichert   mAbs

The Impact II, a Very High-Resolution Quadrupole Time-of-Flight Instrument (QTOF) for Deep Shotgun Proteomics

(2015) Scarlet Beck et al.   MOLECULAR & CELLULAR PROTEOMICS

New Approach for Pseudo-MS3 Analysis of Peptides and Proteins via MALDI In-Source Decay Using Radical Recombination with 1,5-Diaminonaphthalene

(2014) Daiki Asakawa et al.   ANALYTICAL CHEMISTRY

Advantages of Extended Bottom-Up Proteomics Using Sap9 for Analysis of Monoclonal Antibodies

(2014) Kristina Srzentić et al.   ANALYTICAL CHEMISTRY

Middle-Down Analysis of Monoclonal Antibodies with Electron Transfer Dissociation Orbitrap Fourier Transform Mass Spectrometry

(2014) Luca Fornelli et al.   ANALYTICAL CHEMISTRY

A new tool for monoclonal antibody analysis

(2014) Yan An et al.   mAbs

The first pilot project of the consortium for top-down proteomics: A status report

(2014) Xibei Dang et al.   PROTEOMICS

Top-down analysis of 30–80 kDa proteins by electron transfer dissociation time-of-flight mass spectrometry

(2013) Luca Fornelli et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Top-Down Structural Analysis of an Intact Monoclonal Antibody by Electron Capture Dissociation-Fourier Transform Ion Cyclotron Resonance-Mass Spectrometry

(2013) Yuan Mao et al.   ANALYTICAL CHEMISTRY

Correct primary structure assessment and extensive glyco-profiling of cetuximab by a combination of intact, middle-up, middle-down and bottom-up ESI and MALDI mass spectrometry techniques

(2013) Daniel Ayoub et al.   mAbs

Use of 1,5-diaminonaphthalene to combine matrix-assisted laser desorption/ionization in-source decay fragmentation with hydrogen/deuterium exchange

(2013) Pascale Lemaire et al.   RAPID COMMUNICATIONS IN MASS SPECTROMETRY

Determination of Deamidation Artifacts Introduced by Sample Preparation Using 18O-Labeling and Tandem Mass Spectrometry Analysis

(2012) Yi Du et al.   ANALYTICAL CHEMISTRY

Analysis of Intact Monoclonal Antibody IgG1 by Electron Transfer Dissociation Orbitrap FTMS

(2012) Luca Fornelli et al.   MOLECULAR & CELLULAR PROTEOMICS

Structural Analysis of Intact Monoclonal Antibodies by Electron Transfer Dissociation Mass Spectrometry

(2011) Yury O. Tsybin et al.   ANALYTICAL CHEMISTRY

Top-Down Sequencing of O-Glycoproteins by In-Source Decay Matrix-Assisted Laser Desorption Ionization Mass Spectrometry for Glycosylation Site Analysis

(2011) Franz-Georg Hanisch   ANALYTICAL CHEMISTRY

Biosimilar, biobetter and next generation therapeutic antibodies

(2011) Alain Beck   mAbs

Top-Downde NovoProtein Sequencing of a 13.6 kDa Camelid Single Heavy Chain Antibody by Matrix-Assisted Laser Desorption Ionization-Time-of-Flight/Time-of-Flight Mass Spectrometry

(2010) Anja Resemann et al.   ANALYTICAL CHEMISTRY

New Advances in the Understanding of the In-Source Decay Fragmentation of Peptides in MALDI-TOF-MS

(2010) Kevin Demeure et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Potential aggregation prone regions in biotherapeutics

(2010) Xiaoling Wang et al.   mAbs

Sugar-free Antibodies-The Bacterial Solution to Autoimmunity?

(2009) Maria Allhorn et al.   Annals of the New York Academy of Sciences

Direct sequence analysis of proteins by in-source fragmentation during delayed ion extraction

(2009) John J. Lennon et al.   PROTEIN SCIENCE

Immunopurification and Mass Spectrometric Quantification of the Active Form of a Chimeric Therapeutic Antibody in Human Serum

(2008) Mathieu Dubois et al.   ANALYTICAL CHEMISTRY

Automated Proteomics ofE. colivia Top-Down Electron-Transfer Dissociation Mass Spectrometry

(2008) Maureen K. Bunger et al.   ANALYTICAL CHEMISTRY

Combining Bottom-Up and Top-Down Mass Spectrometric Strategies for De Novo Sequencing of the Crustacean Hyperglycemic Hormone from Cancer borealis

(2008) Mingming Ma et al.   ANALYTICAL CHEMISTRY

Development of Novel Protein Scaffolds as Alternatives to Whole Antibodies for Imaging and Therapy: Status on Discovery Research and Clinical Validation

(2008) T. Wurch et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Toward top-down determination of PEGylation site using MALDI in-source decay MS analysis

(2008) Chul Yoo et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY