Article
Biochemistry & Molecular Biology
Hye Lim Choi, Ha Rim Yang, Ha Gyeong Shin, Kyusang Hwang, Ji Woong Kim, Ji Hyun Lee, Taehoon Ryu, Yushin Jung, Sukmook Lee
Summary: Antibody phage display is a crucial technology for discovering and developing target-specific monoclonal antibodies. This study constructed a large human combinatorial single-chain variable fragment library with high diversity, which can be used for the rapid development of recombinant human monoclonal antibodies for therapeutic and diagnostic applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Microbiology
Su Jin Jo, Jun Kwon, Sang Guen Kim, Seung-Jun Lee
Summary: Bacterial infections have become a global threat, and bacteriophages are gaining attention as promising alternatives due to their distinctive attributes and genetic modification abilities. Phage engineering can enhance diagnostics, drug delivery, and therapy, and holds promise in transforming precision medicine, addressing antibiotic resistance, and advancing diverse applications.
Review
Microbiology
Ana S. S. Andre, Isa Moutinho, Joana N. R. Dias, Frederico Aires-da-Silva
Summary: The discovery of hybridoma technology and the development of phage display technology have opened a new era in antibody research and clinical development. Over the past thirty years, display selection technologies such as phage display have been widely promoted as ideal alternatives to traditional methods, with significant advancements in design, construction, and selection of antibody libraries. With the emergence of new therapeutic antibodies, phage display is expected to continue playing a central role, especially in challenging cases. In vivo phage display methods offer an advantage over conventional in vitro procedures for generating therapeutic antibodies against difficult targets and unmet medical needs.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Oladapo Olaleye, Natalia Govorukhina, Nico C. van de Merbel, Rainer Bischoff
Summary: This review discusses the applications of non-antibody-based binders in enriching proteins for LC-MS analysis, comparing them to antibodies and introducing methods for binder immobilization. While the combination of non-antibody-based binders with targeted mass spectrometry shows promise in certain areas, the limited commercial availability of these binders poses a bottleneck that needs to be addressed.
Review
Virology
Kazuya Nagano, Yasuo Tsutsumi
Summary: Antibody drugs with high affinity and specificity are effective for intractable diseases, such as cancers and autoimmune diseases. The recent breakthrough in antibody drugs is largely due to the contribution of phage display technology.
Article
Chemistry, Multidisciplinary
Florian Leonardus Rudolfus Lucas, Kumar Sarthak, Erica Mariska Lenting, David Coltan, Nieck Jordy van der Heide, Roderick Corstiaan Abraham Versloot, Aleksei Aksimentiev, Giovanni Maglia
Summary: This study enhanced the capture frequency and discrimination of peptides by introducing aromatic amino acids into nanopores. Molecular dynamics simulations identified the sensing region and microscopic mechanisms of nanopores, as well as the effects of pore modification on peptide discrimination in FraC.
Article
Biochemistry & Molecular Biology
Lili Li, Meiling Gao, Peng Jiao, Shulong Zu, Yong-qiang Deng, Dingyi Wan, Yang Cao, Jing Duan, Saba R. Aliyari, Jie Li, Yueyue Shi, Zihe Rao, Cheng-feng Qin, Yu Guo, Genhong Cheng, Heng Yang
Summary: This study identified and engineered neutralizing antibodies against SARS-CoV-2 variants, using an antibody phage display library made from COVID-19 patients. One specific antibody clone, R3P1-E4, was found to effectively suppress SARS-CoV-2 infection and rescue lethal phenotypes in infected mice. Crystal structural analysis revealed the reasons behind the reduced binding and neutralizing activity of R3P1-E4 towards SARS-CoV-2 variants with K417 mutations, and allowed for the engineering of mutant antibodies with improved neutralizing activity. This research provides a strategy for identifying and improving neutralizing antibodies against SARS-CoV-2 variants.
CELL AND BIOSCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Seyedeh Zahra Bahojb Mahdavi, Fatemeh Oroojalian, Shirin Eyvazi, Maryam Hejazi, Behzad Baradaran, Nasser Pouladi, Mohammad Reza Tohidkia, Ahad Mokhtarzadeh, Serge Muyldermans
Summary: Antibodies, as therapeutic and diagnostic molecules, play a crucial role in disease treatment, especially in cancer therapy. Alternative approaches, such as display selection technologies, have been developed to overcome the limitations of traditional hybridoma technology. These methods, including phage display, bacterial display, and yeast display, enable the screening and generation of high affinity antibodies with low immunogenicity risk. Understanding the basics of each method is important for antibody design suitable for different diseases, including cancer.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Mohammad Ridhuan Mohd Ali, Jia Siang Sum, Nurul Najian Aminuddin Baki, Yee Siew Choong, Nur Asyura Nor Amdan, Fairuz Amran, Theam Soon Lim
Summary: This study aimed to develop specific monoclonal antibodies against LipL21 antigen for future use in rapid and accurate immunoassay for leptospirosis. The generated monoclonal antibody against rLipL21 will be evaluated as a detection reagent for the diagnosis of human leptospirosis in a future study.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Jingru Liang, Hang Dong, Fei Xu, Baowei Li, Haimei Li, Limei Chen, Mei Li, Yingchu Liu, Guosheng Jiang, Jinhua Dong
Summary: In this study, a monoclonal antibody named ESC9 was developed, and an immunosensor called ESC9 Quenchbody (Q-body) was generated for rapid and sensitive detection of estradiol. The ESC9 Q-body showed potential for clinical applications due to its excellent detection speed and sensitivity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Ana G. Abril, Monica Carrera, Karola Boehme, Jorge Barros-Velazquez, Benito Canas, Jose-Luis R. Rama, Tomas G. Villa, Pilar Calo-Mata
Summary: This study utilized LC-ESI-MS/MS MS to analyze peptides from bacteriophages infecting mastitis-causing Staphylococcus aureus, identifying a large number of specific peptides that could be useful for identifying and characterizing the bacteria causing mastitis. Additionally, a study of bacteriophage phylogeny and its relationship with the respective Staphylococcus spp. infected was conducted, revealing specific peptides present in closely related phages and highlighting the connection between bacteriophage phylogeny and the infected Staphylococcus spp.
Article
Oncology
Kamal Mandal, Gianina Wicaksono, Clinton Yu, Jarrett J. Adams, Michael R. Hoopmann, William C. Temple, Adila Izgutdina, Bonell Patino Escobar, Maryna Gorelik, Christian H. Ihling, Matthew A. Nix, Akul Naik, William H. Xie, Juwita Hubner, Lisa A. Rollins, Sandy M. Reid, Emilio Ramos, Corynn Kasap, Veronica Steri, Juan Antonio Camara Serrano, Fernando Salangsang, Paul Phojanakong, Melanie McMillan, Victor Gavallos, Andrew D. Leavitt, Aaron C. Logan, Cliona M. Rooney, Justin Eyquem, Andrea Sinz, Benjamin J. Huang, Elliot Stieglitz, Catherine C. Smith, Robert L. Moritz, Sachdev S. Sidhu, Lan Huang, Arun P. Wiita
Summary: This study explores a new strategy called structural surfaceomics for identifying and targeting cancer-specific surface protein conformations on tumor cells. The researchers successfully applied this technology to acute myeloid leukemia (AML) and identified an AML-specific target. They developed antibodies to this protein conformation and demonstrated that chimeric antigen receptor T cells can eliminate AML cells without toxicity to normal cells.
Article
Immunology
Ahmad Najafi, Reza Valadan, Hossein Asgarian-Omran, Alireza Rafiei, Mohsen Tehrani
Summary: In this study, a recombinant phage was generated using the phage display method to select monoclonal phages that can react with the dimerization domain of HER2 receptor. Functional tests confirmed their specificity and anti-tumor properties.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Medicine, Research & Experimental
Francesco Nannini, Lenart Senicar, Farhaan Parekh, Khai J. Kong, Alexander Kinna, Reyisa Bughda, James Sillibourne, Xihao Hu, Biao Ma, Yuchen Bai, Mathieu Ferrari, Martin A. Pule, Shimobi C. Onuoha
Summary: Phage display technology combined with next-generation sequencing is a cutting-edge method for monoclonal antibody isolation. Utilizing third-generation sequencing, this study successfully identified functional scFv antibodies from diverse libraries against CD160 and CD123 that were previously undetected by conventional screening methods.
Article
Oncology
Jaline Broqueza, Chandra B. Prabaharan, Samitha Andrahennadi, Kevin J. H. Allen, Ryan Dickinson, Valerie MacDonald-Dickinson, Ekaterina Dadachova, Maruti Uppalapati
Summary: The overexpression of IGF2R in almost all standard and patient-derived OS cell lines makes it an ideal target for antibody-based drugs. Monoclonal antibodies targeting IGF2R can be conjugated with radionuclides to deliver radiation to OS cells, potentially leading to a novel treatment for the disease.