Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 73, Issue -, Pages 108-116Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.lwt.2016.05.049
Keywords
Pleurotus eryngii residue polysaccharides; Response surface methodology; Antioxidant; Cytotoxicity
Categories
Funding
- Special Fund for Agro-scientific Research in the Public Interest [201303080]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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Response surface methodology was used to optimize the extraction of polysaccharides Pleurotus eryngii residue (CPPS). The yield of CPPS was 10.69 +/- 0.31% under the modified condition (leaching temperature of 70 degrees C, leaching time of 140 min'and ratio of water to raw material of 25 mL/g). Ultrafiltration technology was used for further purification of polysaccharides and two fractions of CPPS-1 and CPPS-2 with different molecular weight ranges were obtained. The antioxidant and cytotoxicities of CPPS-1, CPPS-2 and CPPS were determined in vitro. The results showed that CPPS-1 had the strongest activity. Western blotting results showed that the expression of tumor suppressor p53 and apoptosis activator Bax were up-regulated by CPPS-1 (PEPE-3) fraction while the expression of Bcl-2 was down-regulated. Our results suggested that the antitumor activity of PEPE-3 may be related to its capability in inducing apoptosis via activation of mitochondria apoptosis pathway. (C) 2016 Elsevier Ltd. All rights reserved.
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