Article
Cell Biology
Zhimin Qi, Huihui Ji, Monika Le, Hanmei Li, Angela Wieland, Sonja Bauer, Li Liu, Michael Wink, Ingrid Herr
Summary: Sulforaphane extends the lifespan of C. elegans by modulating the insulin/IGF-1 signaling pathway, promoting healthspan by increasing mobility and appetite while reducing lipofuscin accumulation.
Article
Biochemistry & Molecular Biology
Jiahao Zhu, Yumei Jia, Cong Wang, Wangting Zhou, Yifan Shu, Kaixin Zhang, Xiaoxiong Zeng, Renpeng Guo
Summary: This study investigated the anti-aging activities of Lonicera japonica polysaccharides (LJPs) and revealed their mechanism of action. LJPs were found to prolong the lifespan of Caenorhabditis elegans, improve its fitness, reduce lipofuscin accumulation, and activate stress-related genes and antioxidant system. These effects were mediated by the DAF-16 pathway.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Fangru Wang, Ting Jin, Hongyuan Li, Houfang Long, Ying Liu, Sha Jin, Yuyuan Lu, Yinghua Peng, Cong Liu, Lihui Zhao, Xiaohui Wang
Summary: Cannabidivarin (CBDV), a structural analog of cannabidiol (CBD), inhibits the aggregation of alpha-synuclein (alpha-syn) and protects DAergic neurons from oxidative stress and degeneration in a Caenorhabditis elegans model. The phenolic hydroxyl groups of CBDV are essential for its antioxidant activity and the prevention of alpha-syn aggregation.
Article
Environmental Sciences
Chi-Wei Huang, Wan-Ru Liao, Chun Ming How, Pei-Ling Yen, Chia-Cheng Wei
Summary: The study found that ZEN significantly decreased locomotive behaviors in C. elegans. Lifespan and aging markers were also adversely affected by ZEN, which increased ROS levels and downregulated antioxidant genes. Long-term exposure to ZEN induced aging-related decline in C. elegans, associated with oxidative stress and inhibition of antioxidant defense.
ENVIRONMENTAL POLLUTION
(2021)
Article
Pharmacology & Pharmacy
Zhenghan Dong, Yachao Wang, Cuiting Hao, Yuan Cheng, Xi Guo, Yanyu He, Yueyue Shi, Shuang Wang, Yunqi Li, Wei Shi
Summary: This study found that the extract of S. Sanghuang (SSE) has positive effects on nematode indicators. Different concentrations of SSE can prolong the lifespan of nematodes and increase it by 26.41%. SSE also reduces the accumulation of endogenous aging substances, increases antioxidant capacity and enhances physical fitness. RT-PCR analysis shows that SSE treatment upregulates the expression of a series of genes, prolonging the lifespan of nematodes. This study reveals the new role of S. Sanghuang in promoting longevity and inhibiting stress, providing a theoretical basis for its application in anti-aging treatments.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Ji Kent Kwah, Aimee Jaramillo-Lambert
Summary: The article describes a method to determine embryonic viability and brood size in Caenorhabditis elegans. This technique can be used to assess errors in meiosis, fertilization, and embryogenesis and is easily adoptable for new researchers. It is an excellent model organism for studying these processes.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Chemistry, Medicinal
Wenqi Wang, Xin Feng, Yu Du, Cen Liu, Xinxin Pang, Kunxiu Jiang, Xirui Wang, Yonggang Liu
Summary: By modifying pinocembrin with amino acids, the derivative pb-3 showed promising antiaging effects in increasing tolerance to thermal and oxidative stress, as well as reducing lipofuscin accumulation in Caenorhabditis elegans. The antioxidant and antiaging properties of pb-3 may involve the DAF-16/FOXO transcription process. These findings suggest that pinocembrin amino acid derivatives could be a novel agent for antiaging therapy.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)
Article
Food Science & Technology
Jiaqian Hong, Yiming Song, Jiayan Xie, Jianhua Xie, Yi Chen, Ping Li, Danyang Liu, Xiaobo Hu, Qiang Yu
Summary: This study used Caenorhabditis elegans as a model organism to investigate the toxic effects and mechanisms of acrolein (ACR) exposure. The results showed that ACR exposure shortened the lifespan of C. elegans, reduced reproductive capacity and locomotive behavior, and increased oxidative stress levels. ACR also affected the expression of antioxidant-related genes. The findings suggest that ACR exposure induces aging and oxidative stress in C. elegans, activating the DAF-16/FOXO pathway.
Article
Biochemistry & Molecular Biology
Abdel Fawaz Bagoudou, Yifeng Zheng, Masahiro Nakabayashi, Saroat Rawdkuen, Hyun-Young Park, Dhiraj A. Vattem, Kenji Sato, Soichiro Nakamura, Shigeru Katayama
Summary: The study identified the neuroprotective activity of Glochidion littorale leaf extract, which showed high bioactivities in ameliorating oxidative stress and protecting against neurodegeneration in Caenorhabditis elegans. The observed effects may be associated with the activation of the transcription factor DAF-16, and potential key phenolic compounds identified in the extract include myricetin, coumestrin, chlorogenic acid, and hesperidin. This novel neuroprotective activity of GLE may be utilized in developing treatments for neurodegenerative diseases like Parkinson's syndrome.
Article
Biochemistry & Molecular Biology
Yaoru Yu, Jing Chen, Lu An, Tianci Huang, Wenbo Wang, Ziqi Cheng, Lu Wang, Xuesong Xu, Zhizhuang Zhao, Xueqi Fu, Junfeng Ma
Summary: By investigating the structure and biological functions of phosphatases in C. elegans, it was found that suppression of PRL-1 prolonged the lifespan and improved the healthspan of the worms. This effect is mediated through a DAF-16-dependent pathway and is associated with reduced levels of reactive oxygen species (ROS).
Article
Biochemistry & Molecular Biology
Zong-Ping Zhang, Xue Bai, Wen-Bo Cui, Xiao-Han Chen, Xu Liu, De-Juan Zhi, Zhan-Xin Zhang, Dong-Qing Fei, Dong-Sheng Wang
Summary: This study found that Caesalmin C can delay the progression of AD in the transgenic C. elegans model by inhibiting A beta deposition, upregulating the expression of genes associated with oxidative stress, inhibiting acetylcholinesterase activity, and upregulating proteasome activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Maria Alejandra Guerrero-Rubio, Samanta Hernandez-Garcia, Francisco Garcia-Carmona, Fernando Gandia-Herrero
Summary: Flavonoids, potential nutraceutical compounds found in dairy food, are considered health-promoting compounds with promising therapeutic effects on neurological, inflammatory diseases, diabetes, and cancer. In Caenorhabditis elegans, chrysin, 6-hydroxyflavone, and baicalein were found to significantly extend lifespan, with effects mediated by different signaling pathways. Microarray analysis showed that baicalein downregulates important age-related genes such as mTOR and PARP.
Article
Engineering, Environmental
Chan-Wei Yu, Yi-Chun Wu, Vivian Hsiu-Chuan Liao
Summary: The study revealed that early developmental exposure to polystyrene NPs disrupts circadian rhythms in C. elegans, affecting chemotaxis response levels. Furthermore, NPs exposure leads to increased bioaccumulation in entrained worms and production of reactive oxygen species, which are related to disturbed circadian rhythms. The research also showed that the circadian disruption induced by NPs is associated with a decline in stress resistance and is modulated by transcription DAF-16/FOXO signaling.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Biology
Jie Tang, Yi-Cheng Ma, Yuan-Li Chen, Rui-Qiu Yang, Heng-Chen Liu, Xin Wang, Baosen Ni, Cheng-Gang Zou, Ke-Qin Zhang
Summary: The maintenance of proteostasis is crucial for cellular and organism healthspan, yet the impact of proteostasis collapse on reproductive span is not fully understood. In Caenorhabditis elegans, excess accumulation of vitellogenins, major components in yolk proteins, is vital for embryonic development and occurs during the aging process. Our study reveals that vitellogenin accumulation leads to reproductive cessation by impairing lysosomal activity in the intestine and germline. Lysosomal function in the germline is necessary for reproductive span by maintaining oocyte quality. Autophagy and sperm depletion are not involved in vitellogenin accumulation-induced reproductive aging. These findings provide insights into the influence of proteome imbalance on reproductive aging and suggest that improving lysosomal function could be an effective intervention for maintaining reproductive health in mammals.
SCIENCE CHINA-LIFE SCIENCES
(2023)
Article
Developmental Biology
Atrayee Basu, Sibaram Behera, Smriti Bhardwaj, Shirshendu Dey, Anindya Ghosh-Roy
Summary: The adult nervous system has limited capacity to regenerate after accidental damage, requiring proper targeting of the injured axon to its postsynaptic cell. Coordinated insulin signaling activities in neuron and muscle promote "ventral targeting" for functional recovery. This study reveals the important role of insulin signaling in regulating axon guidance molecules during the functional rewiring process.
Article
Neurosciences
Marina Lopes Machado, Leticia Priscilla Arantes, Tassia Limana da Silveira, Daniele Coradini Zamberlan, Larissa Marafiga Cordeiro, Fabiane Baptista Bicca Obetine, Aline Franzen da Silva, Ivana Beatrice Manica da Cruz, Felix Alexandre Antunes Soares, Riva de Paula Oliveira
Summary: The study demonstrated that the hydroalcoholic extract of Ilex paraguariensis and its caffeine component have neuroprotective effects by increasing lifespan, scavenging reactive oxygen species, reducing A beta-induced paralysis and AChE activity, with IPHE's protection also involving hsf-1 and daf-16 proteins.
NUTRITIONAL NEUROSCIENCE
(2021)
Article
Neurosciences
Barbara Nunes Krum, Airton C. Martins, Libania Queiros, Beatriz Ferrer, Ginger L. Milne, Felix Alexandre Antunes Soares, Roselei Fachinetto, Michael Aschner
Summary: The antagonism of the D2-type DA receptor dop-3 by haloperidol is likely responsible for the effects seen in Caenorhabditis elegans, affecting mechanistic pathways involved in neurotransmitter modulation and signaling, such as octopamine, acetylcholine, and GABA. This finding suggests a potential mechanism underlying the effects of haloperidol, with implications for disorders in both invertebrates and vertebrates.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Jose Luiz Portela, Matheus Chimelo Bianchini, Daniel H. Roos, Daiana Silva de Avila, Robson Luiz Puntel
Summary: Supplementation with caffeic acid and caffeine from Ilex paraguariensis offers protection against toxicity induced by methylmalonic or malonic acid exposure in fruit flies, restoring biochemical parameters and behavioral changes affected by the exposure.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Mara Yone D. Fernandes, Fernando Dobrachinski, Henrique B. Silva, Joao Pedro Lopes, Francisco Q. Goncalves, Felix A. A. Soares, Lisiane O. Porciuncula, Geanne M. Andrade, Rodrigo A. Cunha, Angelo R. Tome
Summary: The study demonstrates that chlorogenic acids do not directly affect synaptic transmission and plasticity, but can indirectly correct synaptic dysfunction by affecting other cellular targets. Therefore, chlorogenic acids may serve as a novel neuroprotective strategy, offering new opportunities for therapeutic interventions.
SCIENTIFIC REPORTS
(2021)
Article
Neurosciences
Larissa Marafiga Cordeiro, Marcell Valandro Soares, Aline Franzen da Silva, Marina Lopes Machado, Fabiane Bicca Obetine Baptista, Tassia Limana da Silveira, Leticia Priscilla Arantes, Felix Alexandre Antunes Soares
Summary: The study showed that rutin can maintain the function of neurons and reduce degeneration in a Huntington's disease model. Rutin may achieve this by regulating antioxidant enzymes and other chaperones, demonstrating its potential neuroprotective role.
NUTRITIONAL NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
A. M. Varao, J. D. S. Silva, L. O. Amaral, L. L. P. Aleixo, A. Onduras, C. S. Santos, L. P. D. Silva, D. E. Ribeiro, J. L. L. Filho, J. Bornhorst, M. Stiboller, T. Schwerdtle, L. C. Alves, F. A. A. Soares, P. Gubert
Summary: The study evaluated the toxic effects of thallium acetate on early larval stages of C. elegans, finding that it caused reduced survival, delayed development, behavioral changes, oxidative stress responses, and reproductive damage. Additionally, the study showed that C. elegans has the ability to absorb and bioaccumulate thallium.
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
(2021)
Article
Environmental Sciences
Marcell Valandro Soares, Juliana Mesadri, Debora Farina Goncalves, Larissa Marafiga Cordeiro, Aline Franzen da Silva, Fabiane Bicca Obetine Baptista, Roger Wagner, Cristiane Lenz Dalla Corte, Felix Alexandre Antunes Soares, Daiana Silva Avila
Summary: The study investigated the neurotoxic effects of toluene using a Caenorhabditis elegans model, finding a reduction in neuron fluorescence and morphologic alterations, especially in dopaminergic neurons, after exposure to toluene. The study also suggested that mitochondria may be involved in toluene-induced neurotoxicity, leading to irreversible dopaminergic neuronal death and neurobehavioral impairments.
ENVIRONMENTAL POLLUTION
(2022)
Article
Chemistry, Multidisciplinary
Gustavo Richter Vaz, Mariana Correa Falkembach Carrasco, Matheus Monteiro Batista, Paula Alice Bezerra Barros, Meliza da Conceicao Oliveira, Ana Luiza Muccillo-Baisch, Virginia Campello Yurgel, Francesca Buttini, Felix Alexandre Antunes Soares, Larissa Marafiga Cordeiro, Flavia Fachel, Helder Ferreira Teixeira, Juliana Bidone, Patricia Diaz de Oliveira, Fabio Sonvico, Cristiana Lima Dora
Summary: This study developed omega-3 fatty acid nanoemulsions loaded with CUR and QU for the treatment of brain diseases. The formulations prepared by two different methods exhibited good stability and similar incorporation of CUR and QU. High-pressure homogenization (HPH) was found to be advantageous, and the obtained CQ NE showed potential for sustained release. Toxicity studies showed that the formulations were not toxic for Caenorhabditis elegans. The developed omega-3 fatty acid nanoemulsions have the potential to enhance the nose-to-brain permeation of CUR and QU for improved treatment efficacy.
Article
Biotechnology & Applied Microbiology
P. C. Stibbe, L. B. Ianiski, C. Weiblen, A. F. Maciel, M. L. Machado, T. L. da Silveira, F. A. A. Soares, J. M. Santurio, M. P. Soares, D. I. B. Pereira, L. A. Sangioni, S. de Avila Botton
Summary: The objective of this study was to evaluate the inhibitory effects and toxicity of agricultural fungicides mefenoxam and pyraclostrobin against Pythium insidiosum. The results showed that both compounds had inhibitory effects on the pathogenic oomycete without causing toxicity in vitro and in vivo models. Further studies on pharmacokinetics, toxicity, and pharmacological interactions are necessary for evaluating their potential use in the clinical management of pythiosis.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Cell Biology
Debora F. Goncalves, Tamie Duarte, Joao V. P. Foletto, Leahn R. Senger, Nilda B. V. Brabosa, Felix A. A. Soares, Cristiane L. Dalla Corte
Summary: Parkinson's disease is a common neurodegenerative disease characterized by movement disorders and loss of dopaminergic neurons. Mitochondrial dysfunction and cellular energy imbalance are common markers found in PD. This study used a fruit fly model to investigate the changes in energetic pathways and mitochondrial functions during aging in a PD model.
Article
Biochemistry & Molecular Biology
Thayanara Cruz da Silva, Tassia Limana da Silveira, Luiza Venturini dos Santos, Leticia Priscila Arantes, Rodrigo Pereira Martins, Felix Alexandre Antunes Soares, Cristiane Lenz Dalla Corte
Summary: This study aimed to investigate the effects of adenosine on the behaviors, morphology, survival after stress conditions, and oxidative stress response pathways in Caenorhabditis elegans. The results showed that adenosine modulated the defecation rate, pharyngeal pumping rate, locomotion, and stress response pathways in the worms. Furthermore, adenosine had modulatory effects on oxidative stress response signaling.
NEUROCHEMICAL RESEARCH
(2023)
Article
Toxicology
Luiz Eduardo Ben Pilissao, Cristiane Freitas Rodrigues, Douglas Porto, Gabriela Rossi Brabo, Andressa Tassinari da Silva, Robson Luiz Puntel, Clesio Soldatelli Paim, Cristiane Casagrande Denardin, Daiana Silva Avila
Summary: This study investigated the effects and mechanisms by which maca affects the reproductive system using an in vivo model. It was found that maca extract caused a significant decrease in total number of eggs and in the number of eggs per worm in nematodes, and was associated with increased lipid peroxidation, reduced triacylglycerol levels, impaired vitellogenin expression, and an increase in apoptotic germline cells. This maca extract also contained quantifiable levels of alkaloids.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Article
Neurosciences
Aline Franzen da Silva, Larissa Marafiga Cordeiro, Marcell Vallandro Soares, Danielle Coradini Zamberlan, Fabiane Bicca Obetine Baptista, Tassia Limana da Silveira, Marina Lopes Machado, Leticia Priscilla Arantes, Yanier Nunez-Figueredo, Estael Ochoa Rodriguez, Felix Alexandre Antunes Soares
Summary: A new multi-target molecule, JM-20, has been discovered with potential neuroprotective, antioxidant, and anxiolytic activity. Studies using a C. elegans model have shown that JM-20 affects worm development and behavior, potentially through modulation of the GABAergic system.
Article
Endocrinology & Metabolism
Eduarda Monteiro Fidelis, Anne Suely P. Savall, Jhuly Dornelles Mello, Caroline Brandao Quines, Antonio Alvenir Comis-Neto, Tuane Bazanella Sampaio, Cristiane Casagrande Denardin, Daiana Silva de avila, Suzan Goncalves Rosa, Simone Pinton
Summary: This study investigated the antioxidant and neuroprotective effects of purple pitanga extract (PPE) against early oxidative stress induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropypiridine (MPTP) administration. The findings showed that PPE prevented the increase in reactive oxygen species (ROS) levels and protected against lipid peroxidation caused by MPTP. Furthermore, PPE enhanced the activity of Na+/K+ ATPase, indicating its neuroprotective capacity.
METABOLIC BRAIN DISEASE
(2023)
Article
Medicine, Research & Experimental
Yuwen Zhu, Yan Guo, Yujia Xue, Anqi Zhou, Ying Chen, Yifei Chen, Xiulian Miao, Fangqiao Lv
Summary: BRG1 plays an important role in HSC-myofibroblast transition and targeting it could be a reasonable strategy for liver fibrosis intervention.
Article
Medicine, Research & Experimental
Liu Ye, Beibei Liu, Jingling Huang, Xiaolin Zhao, Yuan Wang, Yungen Xu, Shuping Wang
Summary: Doublecortin-like kinase 1 (DCLK1) is a significant prooncogenic factor that is strongly associated with the malignant progression and clinical prognosis of various cancers. DCLK1 plays important roles in stem cell marker regulation, tumor cell reprogramming, and immune evasion. However, the exact biological functions of DCLK1, especially the disparities between its alpha- and beta-form transcripts in cancer progression, remain ambiguous.
Article
Medicine, Research & Experimental
Jiahui Yang, Xiaoyu Chen, Tianjing Liu, Yongyan Shi
Summary: This article reviews the role of bile acids in necrotizing enterocolitis (NEC) and their potential therapeutic value. The dysregulation of bile acids is associated with intestinal injury, and inflammatory factors in the liver also play a crucial role in regulating bile acid transport. The bile acid metabolic pathway is important for regulating intestinal microbiota, cell proliferation, and barrier protection.
Review
Medicine, Research & Experimental
Zhenzheng Zhu, Yuemiao Xu, Yuwei Xia, Xinru Jia, Yixin Chen, Yuyue Liu, Leyin Zhang, Hui Chai, Leitao Sun
Summary: Bile acid, as the final product of cholesterol breakdown, plays a complex regulatory and signaling role in human metabolism. Research suggests that it has the potential to enhance metabolism and regulate chronic metabolic diseases through various pathways. The interaction between bile acid and gut microbiota is also of great significance.
Article
Medicine, Research & Experimental
Xin He, Hong-Xu Zhou, Xian Fu, Kai-Di Ni, Ai-Zhi Lin, Ling-Tong Zhang, Hou-Hua Yin, Qing Jiang, Xue Zhou, Yi-Wen Meng, Jun-Yan Liu
Summary: DON exposure causes an increase in deoxycholic acid (DCA), which contributes to intestinal injury. DCA may be a potential therapeutic target for DON enterotoxicity.
Article
Medicine, Research & Experimental
Zhitao Wang, Heng Ma, Abdul Nasir, Sufang Liu, Zhisong Li, Feng Tao, Qian Bai
Summary: This study reveals the involvement of TET1-mediated epigenetic regulation in chronic TMJ pain through trigeminal TNF alpha signaling.
Article
Medicine, Research & Experimental
Lu Yu, Hao Ran, Yaru Lu, Qian Ma, Huan Huang, Weibin Liu
Summary: This study found that the HIF-1 alpha inhibitor BAY 87-2243 can alleviate the symptoms of the Experimental Autoimmune Myasthenia Gravis (EAMG) inflammation model. BAY 87-2243 can restore the balance of CD4(+)T cell subsets, reduce the production of pro-inflammatory cytokines, and act as both an immune imbalance regulator and anti-inflammatory.
Article
Medicine, Research & Experimental
Alex Peralvarez-Marin, Montse Sole, Judith Serrano, Alice Taddeucci, Belen Perez, Clara Penas, Gemma Manich, Marcel Jimenez, Pilar D'Ocon, Francesc Jimenez-Altayo
Summary: This study provides the first evidence that TRPV2 channels may modulate vascular tone by balancing opposing inputs from the endothelium and smooth muscle, leading to net vasodilation. The amplification of TRPV2 channel-induced activity by NO emphasizes the pathophysiological relevance of these findings.
Article
Medicine, Research & Experimental
Amin Ullah, Jing Zhao, Jiakun Li, Rajeev K. Singla, Bairong Shen
Summary: Gastric cancer is the fifth-most prevalent and second-most deadly cancer worldwide. Late onset of symptoms makes early detection important. CXC chemokines play an important role in the pathological process of gastric cancer, but their exact role in diagnosis and prognosis is not fully understood. Inhibiting CXC chemokines shows promise as a targeted therapy.
Article
Medicine, Research & Experimental
Menna S. Zeyada, Salma M. Eraky, Mamdouh M. El-Shishtawy
Summary: The current study demonstrates the prophylactic and antifibrotic effects of Trig against BLM-induced PF by targeting multiple signaling pathways. The combination of Trig and Pirf may be a promising approach to enhance Pirf's anti-fibrotic effect.