Chemically modified aptamers for improving binding affinity to the target proteins via enhanced non-covalent bonding

Success probability of high-affinity DNA aptamer generation by genetic alphabet expansion

(2023) Michiko Kimoto et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Structure-Guided Designing Pre-Organization in Bivalent Aptamers

(2022) Xiaoli Hu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A high-affinity aptamer with base-appended base-modified DNA bound to isolated authentic SARS-CoV-2 strains wild-type and B.1.617.2 (delta variant)

(2022) Hirotaka Minagawa et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Directed evolution and selection of biostable l-DNA aptamers with a mirror-image DNA polymerase

(2022) Ji Chen et al.   NATURE BIOTECHNOLOGY

Targeting loop3 of sclerostin preserves its cardiovascular protective action and promotes bone formation

(2022) Yuanyuan Yu et al.   Nature Communications

A Sensitive Aptamer Fluorescence Anisotropy Sensor for Cd2+ Using Affinity-Enhanced Aptamers with Phosphorothioate Modification

(2022) Hao Yu et al.   Biosensors-Basel

EXPANDING THE RECOGNITION INTERFACE OF THE THROMBIN BINDING APTAMER HD1 THROUGH MODIFICATION OF RESIDUES T3 AND T12

(2021) Igor Smirnov et al.   Molecular Therapy-Nucleic Acids

2′-Fluoro-arabinonucleic Acid (FANA): A Versatile Tool for Probing Biomolecular Interactions

(2021) Roberto El-Khoury et al.   ACCOUNTS OF CHEMICAL RESEARCH

Systematic Approach to DNA Aptamer Design Using Amino Acid–Nucleic Acid Hybrids (ANHs) Targeting Thrombin

(2021) Ji Hye Yum et al.   ACS Biomaterials-Science & Engineering

High-affinity five/six-letter DNA aptamers with superior specificity enabling the detection of dengue NS1 protein variants beyond the serotype identification

(2021) Ken-ichiro Matsunaga et al.   NUCLEIC ACIDS RESEARCH

Competitive ELISA for a serologic test to detect dengue serotype-specific anti-NS1 IgGs using high-affinity UB-DNA aptamers

(2021) Ken-ichiro Matsunaga et al.   Scientific Reports

Evolution of Functionally Enhanced α-l-Threofuranosyl Nucleic Acid Aptamers

(2021) Cailen M. McCloskey et al.   ACS Synthetic Biology

In vitro selection of l-DNA aptamers that bind a structured d-RNA molecule

(2020) Sougata Dey et al.   NUCLEIC ACIDS RESEARCH

A high affinity modified DNA aptamer containing base-appended bases for human β-defensin

(2020) Hirotaka Minagawa et al.   ANALYTICAL BIOCHEMISTRY

Generating Biologically Stable TNA Aptamers that Function with High Affinity and Thermal Stability

(2020) Matthew R. Dunn et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Recent Advances in Aptamer Applications for Analytical Biochemistry

(2020) Gerald Zon   ANALYTICAL BIOCHEMISTRY

Evolution of abiotic cubane chemistries in a nucleic acid aptamer allows selective recognition of a malaria biomarker

(2020) Yee-Wai Cheung et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Phosphorothioate nucleic acids for probing metal binding, biosensing and nanotechnology

(2020) Runjhun Saran et al.   COORDINATION CHEMISTRY REVIEWS

Hachimoji DNA and RNA: A genetic system with eight building blocks

(2019) Shuichi Hoshika et al.   SCIENCE

Improved RE31 Analogues Containing Modified Nucleic Acid Monomers: Thermodynamic, Structural, and Biological Effects

(2019) Weronika Kotkowiak et al.   JOURNAL OF MEDICINAL CHEMISTRY

A synthetic genetic polymer with an uncharged backbone chemistry based on alkyl phosphonate nucleic acids

(2019) Sebastian Arangundy-Franklin et al.   Nature Chemistry

Highly stable hexitol based XNA aptamers targeting the vascular endothelial growth factor

(2019) Elena Eremeeva et al.   NUCLEIC ACIDS RESEARCH

Structure-guided post-SELEX optimization of an ochratoxin A aptamer

(2019) Guohua Xu et al.   NUCLEIC ACIDS RESEARCH

Selection of 2'-Deoxy-2'-Fluoroarabino Nucleic Acid (FANA) Aptamers that Bind HIV-1 Integrase with Picomolar Affinity

(2019) Kevin M. Rose et al.   ACS Chemical Biology

Aptamers Chemistry: Chemical Modifications and Conjugation Strategies

(2019) Fadwa Odeh et al.   MOLECULES

Aptamer chemistry

(2018) Pascal Röthlisberger et al.   ADVANCED DRUG DELIVERY REVIEWS

Modulation of BACE1 Activity by Chemically Modified Aptamers

(2018) Cécile Gasse et al.   CHEMBIOCHEM

Identification and characterization of nucleobase-modified aptamers by click-SELEX

(2018) Franziska Pfeiffer et al.   Nature Protocols

Gemcitabine-incorporated G-quadruplex aptamer for targeted drug delivery into pancreas cancer

(2018) Jun Young Park et al.   Molecular Therapy-Nucleic Acids

Creation of unnatural base pairs for genetic alphabet expansion toward synthetic xenobiology

(2018) Kiyofumi Hamashima et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Aptamer-Mediated Delivery of Splice-Switching Oligonucleotides to the Nuclei of Cancer Cells

(2018) Jonathan W. Kotula et al.   Nucleic Acid Therapeutics

Phosphorothioate Modified AP613-1 Used for Hepatocellular Carcinoma Cell Imaging Via Specifically Targeting GPC3

(2018) Lili Dong et al.   Molecular Therapy-Nucleic Acids

Tethered imidazole mediated duplex stabilization and its potential for aptamer stabilization

(2018) Lars Verdonck et al.   NUCLEIC ACIDS RESEARCH

Mapping the affinity landscape of Thrombin-binding aptamers on 2′F-ANA/DNA chimeric G-Quadruplex microarrays

(2017) Jory Lietard et al.   NUCLEIC ACIDS RESEARCH

Selection of DNA aptamers with two modified bases

(2017) Bharat N. Gawande et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Systematic Optimization and Modification of a DNA Aptamer with 2’-O-Methyl RNA Analogues

(2017) George E. Maio et al.   ChemistrySelect

Selection, Characterization and Application of Artificial DNA Aptamer Containing Appended Bases with Sub-nanomolar Affinity for a Salivary Biomarker

(2017) Hirotaka Minagawa et al.   Scientific Reports

Aptamers against Cells Overexpressing Glypican 3 from Expanded Genetic Systems Combined with Cell Engineering and Laboratory Evolution

(2016) Liqin Zhang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Recent advances in drug delivery strategies for improved therapeutic efficacy of gemcitabine

(2016) Ravindra Dhar Dubey et al.   EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES

High-Affinity DNA Aptamer Generation Targeting von Willebrand Factor A1-Domain by Genetic Alphabet Expansion for Systematic Evolution of Ligands by Exponential Enrichment Using Two Types of Libraries Composed of Five Different Bases

(2016) Ken-ichiro Matsunaga et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enhancing aptamer function and stability via in vitro selection using modified nucleic acids

(2016) Kirsten N. Meek et al.   METHODS

Methods for Improving Aptamer Binding Affinity

(2016) Hijiri Hasegawa et al.   MOLECULES

Laboratory evolution of artificially expanded DNA gives redesignable aptamers that target the toxic form of anthrax protective antigen

(2016) Elisa Biondi et al.   NUCLEIC ACIDS RESEARCH

Evoking picomolar binding in RNA by a single phosphorodithioate linkage

(2016) N. Dinuka Abeydeera et al.   NUCLEIC ACIDS RESEARCH

Post-ExSELEX stabilization of an unnatural-base DNA aptamer targeting VEGF165toward pharmaceutical applications

(2016) Michiko Kimoto et al.   NUCLEIC ACIDS RESEARCH

In vitro evolution of chemically-modified nucleic acid aptamers: Pros and cons, and comprehensive selection strategies

(2016) Farhana Lipi et al.   RNA Biology

A Versatile Approach Towards Nucleobase-Modified Aptamers

(2015) Fabian Tolle et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Replacing antibodies with aptamers in lateral flow immunoassay

(2015) Ailiang Chen et al.   BIOSENSORS & BIOELECTRONICS

Turning mirror-image oligonucleotides into drugs: the evolution of Spiegelmer® therapeutics

(2015) Axel Vater et al.   DRUG DISCOVERY TODAY

Selection of 2′-deoxy-2′-fluoroarabinonucleotide (FANA) aptamers that bind HIV-1 reverse transcriptase with picomolar affinity

(2015) Irani Alves Ferreira-Bravo et al.   NUCLEIC ACIDS RESEARCH

2′-Fluoro-modified phosphorothioate oligonucleotide can cause rapid degradation of P54nrb and PSF

(2015) Wen Shen et al.   NUCLEIC ACIDS RESEARCH

Adipo8, a high-affinity DNA aptamer, can differentiate among adipocytes and inhibit intracellular lipid accumulation in vitro

(2015) Ke Chen et al.   Science China-Chemistry

Architecture of high-affinity unnatural-base DNA aptamers toward pharmaceutical applications

(2015) Ken-ichiro Matsunaga et al.   Scientific Reports

Identification of a pKa-regulating motif stabilizing imidazole-modified double-stranded DNA

(2014) Dieter Buyst et al.   NUCLEIC ACIDS RESEARCH

Nucleic Acid Ligands With Protein-like Side Chains: Modified Aptamers and Their Use as Diagnostic and Therapeutic Agents

(2014) John C Rohloff et al.   Molecular Therapy-Nucleic Acids

Backbone FCH⋅⋅⋅O Hydrogen Bonds in 2′F-Substituted Nucleic Acids

(2013) Nerea Martin-Pintado et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Inhibition of human neutrophil activity by an RNA aptamer bound to interleukin-8

(2013) Ho Jin Sung et al.   BIOMATERIALS

Hematopoietic Stem and Progenitor Cell Mobilization in Mice and Humans by a First-in-Class Mirror-Image Oligonucleotide Inhibitor of CXCL12

(2013) A Vater et al.   CLINICAL PHARMACOLOGY & THERAPEUTICS

A Novel C5a-neutralizing Mirror-image (l-)Aptamer Prevents Organ Failure and Improves Survival in Experimental Sepsis

(2013) Kai Hoehlig et al.   MOLECULAR THERAPY

Generation of high-affinity DNA aptamers using an expanded genetic alphabet

(2013) Michiko Kimoto et al.   NATURE BIOTECHNOLOGY

Selection of Aptamers Specific for Adipose Tissue

(2012) Jun Liu et al.   PLoS One

Unique motifs and hydrophobic interactions shape the binding of modified DNA ligands to protein targets

(2012) D. R. Davies et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Arenavirus Evasion of Host Anti-Viral Responses

(2012) Melissa Hayes et al.   Viruses-Basel

Locked vs. unlocked nucleic acids (LNA vs. UNA): contrasting structures work towards common therapeutic goals

(2011) Meghan A. Campbell et al.   CHEMICAL SOCIETY REVIEWS

Improving the Stability of Aptamers by Chemical Modification

(2011) R. E. Wang et al.   CURRENT MEDICINAL CHEMISTRY

Expanding the Chemistry of DNA for in Vitro Selection

(2010) Jonathan D. Vaught et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Aptamers as a model for functional evaluation of LNA and 2′-amino LNA

(2009) Frank J. Hernandez et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Investigations toward the selection of fully-modified 4′-thioRNA aptamers: Optimization of in vitro transcription steps in the presence of 4′-thioNTPs

(2008) Noriaki Minakawa et al.   BIOORGANIC & MEDICINAL CHEMISTRY

2′F-Arabinonucleic acids (2′F-ANA) — History, properties, and new frontiers

(2008) Jonathan K Watts et al.   CANADIAN JOURNAL OF CHEMISTRY