Journal
LETTERS IN APPLIED MICROBIOLOGY
Volume 63, Issue 2, Pages 89-95Publisher
WILEY
DOI: 10.1111/lam.12593
Keywords
Acute Oak Decline; Brenneria goodwinii; Gibbsiella quercinecans; High-resolution melt; identification
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Funding
- University of the West of England
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Two Gram-negative Enterobacteriaceae, Gibbsiella quercinecans and Brenneria goodwinii, are frequently isolated from oak suffering from Acute Oak Decline. These two species are difficult to identify based on colony morphology, carbohydrate utilization or 16S rRNA gene sequence, and identification using gyrB gene sequencing is time-consuming and laborious. A rapid identification technique, based on high-resolution melt analysis of the atpD gene, was designed to efficiently process numerous isolates from an increasing number of affected woodlands and parks. Principal component analysis of the resulting melt curves from strains of G.quercinecans, B.goodwinii and their close phylogenetic relatives allowed differentiation into distinct clusters based on species or subspecies identity.
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