Article
Pharmacology & Pharmacy
Roland Schaller-Ammann, Sebastian Kress, Juergen Feiel, Gerd Schwagerle, Joachim Priedl, Thomas Birngruber, Cornelia Kasper, Dominik Egger
Summary: Skin equivalents and skin explants are commonly used for dermal penetration studies. This research developed a sampling design that combines continuous concentration measurements in the tissue with online monitoring of culture parameters, providing real-time data for the experimental outcomes.
Article
Chemistry, Multidisciplinary
Levin Hafa, Louise Breideband, Lucas Ramirez Posada, Nuria Torras, Elena Martinez, Ernst H. K. Stelzer, Francesco Pampaloni
Summary: Tissue engineering is an important and widely applicable field that offers alternatives to animal models and enables tissue regeneration and organ transplantation. 3D bioprinting stands out for its flexibility and reproducibility. A study introduces an integrated fluorescent light sheet bioprinting and imaging system, which allows real-time monitoring of material crosslinking and cell activity, offering high speed, high resolution, and real-time characterization capabilities.
ADVANCED MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Dragan Copic, Maria Laggner, Polina Kalinina, Katharina Klas, Erwin Tschachler, Michael Mildner
Summary: Ichthyoses are a group of keratinization disorders caused by hereditary defects, with mutations in over 50 genes identified as causes. However, the high heterogeneity of the disease and challenges in establishing valid experimental models make research in this field difficult.
Article
Dermatology
Rajiv Raktoe, Anastasia K. A. L. Kwee, Marion Rietveld, Nick Marsidi, Roel Genders, Koen Quint, Remco van Doorn, Paul van Zuijlen, Abdoelwaheb E. L. Ghalbzouri
Summary: Deep burn injuries often result in severe scarring, such as hypertrophic scar formation. Autologous fat grafting, also known as lipofilling, has shown promise in reducing scar formation. This study aims to investigate the effects of fat grafting in 3D organotypic skin cultures resembling a hypertrophic scar-like environment.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Andres Montero, Cristina Quilez, Leticia Valencia, Paula Giron, Jose Luis Jorcano, Diego Velasco
Summary: This study demonstrates that increasing fibrin concentration can significantly improve mechanical properties and matrix stability, thereby promoting the development of in vitro skin cultures. These new systems will provide better in vitro skin models for testing drugs, cosmetics, and chemicals, and can even be used for personalized skin diagnosis and determining the most effective treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Paula Montero, Maria Jose Villarroel, Ines Roger, Anselm Morell, Javier Milara, Julio Cortijo
Summary: Solar radiation can lead to various negative effects on the skin, such as sunburn, inflammation, and DNA damage. One potential approach to photoprotection is by targeting the transcription factor Nrf2, and obacunone has been identified as a potent Nrf2 agonist. This study aims to evaluate the relevance of full-thickness skin models in photoprotection studies and explore the photoprotective effects of obacunone in human keratinocytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Nilakshi Barua, Lin Huang, Carmen Li, Ying Yang, Mingjing Luo, Wan In Wei, Kam Tak Wong, Norman Wai Sing Lo, Kin On Kwok, Margaret Ip
Summary: In this study, a 3D organotypic keratinocyte-fibroblast co-culture model was established to evaluate the effectiveness of a 3D skin model in studying cell death induced by Methicillin-resistant Staphylococcus aureus (MRSA) compared to a 2D HaCaT monolayer. The results showed that the 3D skin model provided better insights into the physiological response of skin to MRSA infection and revealed variations in pathogenesis among different MRSA strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Dermatology
Christianna Marie Czyz, Paul Werner Kunth, Florian Gruber, Christopher Kremslehner, Christoph Matthias Hammers, Jennifer Elisabeth Hundt
Summary: Human skin equivalents (HSEs) are three-dimensional skin organ culture models raised in vitro. This review provides an overview of common techniques for setting up HSEs, including the use of 3T3-J2 murine fibroblasts, purchased human fibroblasts or freshly isolated and cultured fibroblasts to build the scaffold, and freshly isolated and cultured keratinocytes to seed on top. It also discusses the addition of other cell types such as melanocytes and provides possible markers and techniques for evaluating artificial skin. The review further compares HSEs to human skin organ culture and discusses their advantages, limitations, and future perspectives.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Biochemical Research Methods
Nathalia de Carvalho Indolfo, Melissa Dibbernn Ganzerla, Tabata Renee Doratioto, Thayna Mendonca Avelino, Larissa Bueno Tofani, Luis Antonio Peroni, Renata Santos Rabelo, Kelen Fabiola Arroteia, Ana Carolina Migliorini Figueira
Summary: Animal testing for cosmetic ingredients and final products has been banned in Europe and is gaining legal force worldwide. However, reliable testing methods are still needed for safety assessment of cosmetic ingredients. This study presents a three-organ microfluidic microphysiological system (MPS) combined with gene expression analysis as a promising tool to evaluate toxicological endpoints of chemicals.
Article
Oncology
Sanjiban Chakrabarty, William F. Quiros-Solano, Maayke M. P. Kuijten, Ben Haspels, Sandeep Mallya, Calvin Shun Yu Lo, Amr Othman, Cinzia Silvestri, Anja van de Stolpe, Nikolas Gaio, Hanny Odijk, Marieke van de Ven, Corrina M. A. de Ridder, Wytske M. van Weerden, Jos Jonkers, Ronald Dekker, Nitika Taneja, Roland Kanaar, Dik C. van Gent
Summary: This study developed a microfluidic platform for evaluating cancer treatment response and predicting drug efficacy in breast and prostate tumors. By culturing tumor slices under controlled growth conditions, the platform allows reliable assessment of chemotherapeutic sensitivity and enables detailed mechanistic studies.
Article
Medicine, General & Internal
Iwona Niedzielska, Lukasz Obszynski, Michal Bak, Damian Niedzielski
Summary: This study evaluated the benefits of full-thickness skin grafts in the reconstruction of midface defects after maxillectomy. The results showed that full-thickness skin grafts contributed to patient's convalescence and reduced postoperative pain, making them a good alternative for patients who are not eligible for microsurgical treatment.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Immunology
Johanna Maria Hoelken, Katja Friedrich, Marion Merkel, Nelli Blasius, Ursula Engels, Timo Buhl, Karsten Ruediger Mewes, Lars Vierkotten, Nicole Elisabeth Teusch
Summary: In this study, THP-1-derived iDCs were integrated into a human skin model as central mediators of the immune reaction. The researchers found that different treatments of iDCs resulted in changes in proteins and cytokines related to sensitization and anti-sensitization. This provides a new approach for studying sensitizing agents and potential drugs for suppressing sensitization.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Engineering, Biomedical
Ronghua Jin, Yuecheng Cui, Haojiao Chen, Zhenzhen Zhang, Tingting Weng, Sizhan Xia, Meirong Yu, Wei Zhang, Jiaming Shao, Min Yang, Chunmao Han, Xingang Wang
Summary: By using 3D bioprinting technology to create suitable bioinks, cell growth and skin reconstruction can be facilitated. The new skin model holds promise as an effective skin substitute both in vitro and in vivo.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Minwei Yang, Jing Tian, Kaiwen Zhang, Xu Fei, Fawen Yin, Longquan Xu, Yi Wang, Yao Li
Summary: This study designed an adhesive antibacterial hydrogel dressing based on dynamic host-guest interaction, inspired by mussel. The hydrogel showed strong adhesion and on-demand removal, along with rapid self-healing properties. It also exhibited excellent performance in promoting wound healing and reducing scar formation.
Article
Oncology
J. Jason Morton, Nathaniel Alzofon, Stephen B. Keysar, Tugs-Saikhan Chimed, Julie Reisinger, Loni Perrenoud, Phuong N. Le, Cera Nieto, Karina Gomez, Bettina Miller, Randi Yeager, Dexiang Gao, Aik-Choon Tan, Hilary Somerset, Theresa Medina, Xiao-Jing Wang, Jing H. Wang, William Robinson, Dennis R. Roop, Rene Gonzalez, Antonio Jimeno
Summary: The research team constructed an autologous humanized mouse model that successfully replicated the patient-specific tumor microenvironment, showing that tumors in this model grew faster, possibly due to enhanced immune cell activity. By mimicking the immunotherapies received by the original patients, the study found that accelerated tumor growth was associated with decreased immune cell infiltration and reduced interferon gamma-related gene expression.
MOLECULAR CANCER RESEARCH
(2021)
Article
Engineering, Biomedical
Laura Huth, Sebastian Huth, Yvonne Marquardt, Philipp Winterhalder, Timm Steiner, Frank Hoelzle, Reinhard Gansel, Jens Malte Baron, Laurenz Schmitt
LASERS IN MEDICAL SCIENCE
(2021)
Article
Dermatology
Sebastian Huth, Yvonne Marquardt, Laura Huth, Laurenz Schmitt, Kirsten Prescher, Philipp Winterhalder, Timm Steiner, Frank Hoelzle, Michael Eble, Jens Malte Baron
Summary: The study found that aftercare treatment with dexpanthenol-containing ointment or liquid can completely restore the epidermal part of skin models after radiation, exhibiting anti-oxidative and anti-inflammatory effects, and positively influencing epidermal differentiation and structures.
EXPERIMENTAL DERMATOLOGY
(2021)
Article
Engineering, Biomedical
Sebastian Huth, Laura Huth, Yvonne Marquardt, Maria Cheremkhina, Ruth Heise, Jens Malte Baron
Summary: The study investigated the role of MMP-3 and its regulation by calcium pantothenate in wound healing after ablative fractional laser treatment. Results showed that aftercare treatment with calcium pantothenate accelerated wound closure in MMP-3 expressing models faster than in MMP-3 knockdown models. This study provides evidence for the first time that calcium pantothenate promotes wound healing at least partly via MMP-3.
LASERS IN MEDICAL SCIENCE
(2022)
Article
Immunology
Ramona A. A. Eichkorn, Morna F. F. Schmidt, Elias Walter, Michael Hertl, Jens Malte Baron, Jens Waschke, Amir S. S. Yazdi
Summary: This study reveals the molecular mechanisms underlying auto-antibody-induced acantholysis in pemphigus vulgaris and identifies UVA as a cofactor for acantholysis. It demonstrates that UVA induces IL-1 secretion in keratinocytes and enhances the loss of epidermal adhesion mediated by anti-Dsg3 antibodies. These findings suggest that UVA plays a caspase-dependent exogenous role in blister formation in pemphigus vulgaris.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Dermatology
Uwe Paasch, Miriam Zidane, Jens Malte Baron, Thorsten Bund, Hans-Joachim Cappius, Michael Drosner, Konstantin Feise, Tanja Fischer, Gerd Gauglitz, Peter Arne Gerber, Sonja Grunewald, Katharina Herberger, Anja Jung, Syrus Karsai, Gerd Kautz, Carsten Philipp, Daniela Schaedel, Anna-Theresa Seitz, Alexander Nast
Summary: This guideline aims to provide specific treatment recommendations for various skin lesions, ensuring safe and effective laser therapy. It emphasizes the importance of understanding the differences in treatment methods for different types of skin conditions to avoid unnecessary risks.
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2022)
Article
Dermatology
Helena Isabell Riedemann, Morna F. Schmidt, Jens Malte Baron
Summary: A scar is the result of a deep tissue defect during the wound healing process. Disorders can occur at all stages, leading to impaired wound healing or the formation of atrophic and hypertrophic scars or keloids. This article focuses on treating pathologic scars, which may cause functional limitations, complaints, and stigmatization. Medical, physical, surgical, and laser therapeutic approaches are pursued, based on an understanding of pathophysiological mechanisms and a comprehensive therapeutic approach.
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2023)
Article
Dermatology
Katharina Webollek, Yvonne Marquardt, Sylvia Wagner-Schiffler, Jens Malte Baron, Sebastian Huth
Summary: The purpose of this in vitro study was to investigate the molecular effects of dexpanthenol-containing ointment (DCO) after micro-needling. The results showed that DCO accelerated wound healing and did not impair the immunostimulatory properties of micro-needling. These findings can optimize the aftercare routine and reduce downtime for patients after microneedling procedures.
CLINICAL COSMETIC AND INVESTIGATIONAL DERMATOLOGY
(2023)
Article
Allergy
Karsten Weller, Ana Maria Gimenez-Arnau, Jens Baron, Randolf Brehler, Marta Ferrer, Adriane Groffik, Sonja Grundmann, Thilo Jakob, Moises Labrador-Horrillo, Sabine Mueller, Petra Staubach, Gerda Wurpts, Martin Metz, Marcus Maurer
Summary: This study compared on-demand (OD) treatment versus daily maintenance treatment with non-sedating H1-antihistamines for chronic spontaneous urticaria. It also assessed the efficacy of rupatadine updosing and investigated potential long-term disease-modifying effects. The results showed that daily rupatadine treatment significantly improved disease activity and quality of life during treatment, but there was no significant difference after discontinuation of rupatadine.
Article
Dermatology
Helena Isabell Riedemann, Morna F. Schmidt, Jens Malte Baron
Summary: A scar is formed as part of the wound healing process, involving inflammation and the proliferation of connective tissue cells. Pathological scars can result in impaired wound healing or the formation of atrophic and hypertrophic scars or keloids. This article focuses on the treatment of pathological scars using medical, physical, surgical, and laser therapeutic approaches, based on an understanding of the underlying mechanisms and interdisciplinary therapeutic approach.
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2023)
Article
Multidisciplinary Sciences
Sebastian Huth, Laura Huth, Ruth Heise, Yvonne Marquardt, Linda Lopopolo, Marta Piecychna, Peter Boor, Guenter Fingerle-Rowson, Aphrodite Kapurniotu, Amir S. Yazdi, Richard Bucala, Juergen Bernhagen, Jens Malte Baron
Summary: The study found that MIF and D-DT play important roles in the development and progression of UVB-induced non-melanoma skin cancer (NMSC), but have no major effects on chemically-induced skin tumors. These findings suggest that MIF and D-DT are potential therapeutic targets for the prevention of photocarcinogenesis but not chemical carcinogenesis.
SCIENTIFIC REPORTS
(2023)
Article
Allergy
Conny Hoeflich, Galina Balakirski, Zuzanna Hajdu, Jens Malte Baron, Katharina Fietkau, Hans F. Merk, Ulrich Strassen, Henning Bier, Wolfgang Dott, Hans-Guido Muecke, Wolfgang Straff, Gerda Wurpts, Amir S. Yazdi, Adam Chaker, Stefani T. M. Roeseler
Meeting Abstract
Dermatology
Helena Isabell Riedemann, Yvonne Marquardt, Manuela Jansen, Jens Malte Baron, Sebastian Huth
LASERS IN SURGERY AND MEDICINE
(2023)
Meeting Abstract
Cell & Tissue Engineering
Rahul Rimal, Yvonne Marquardt, Suzana Djeljaidni, Thomas Neovalis, Dmitry Chigrin, Matthias Wessling, Jens Malte Baron, Martin Moeller, Smriti Singh
TISSUE ENGINEERING PART A
(2022)
Article
Cell & Tissue Engineering
Rebecca Guenther, Stephan Dreschers, Jessika Maassen, Daniel Reibert, Claudia Skazik-Voogt, Angela Gutermuth
Summary: Postnatal umbilical cord tissue contains highly potent VSELs-like stem cells, which express pluripotency markers and have the ability to differentiate into meso- endo- and ectodermal precursor cells.
STEM CELL REVIEWS AND REPORTS
(2022)
Article
Cell & Tissue Engineering
Christian Olszewski, Jessika Maassen, Rebecca Guenther, Claudia Skazik-Voogt, Angela Gutermuth
Summary: Corneal endothelial insufficiency is a leading cause of blindness. Stem cells derived from hair follicles of older tissue donors show potential for differentiation into corneal endothelial cells, making them a promising alternative for conventional grafts. These highly potent hair follicle derived stem cells may provide autologous corneal endothelial replacement for patients of all ages.
STEM CELL REVIEWS AND REPORTS
(2022)