Journal
CHEMCATCHEM
Volume 7, Issue 9, Pages 1438-1444Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cctc.201500061
Keywords
biotransformations; carboxylic acids; cyanides; enzyme catalysis; gene expression
Categories
Funding
- Key deployment project of Chinese Academy of Sciences [KSED-EW-Z-015]
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An effective one-pot bienzymatic synthesis of ethyl (R)-3-hydroxyglutarate (EHG) from ethyl (S)-4-chloro-3-hydroxybutyrate (ECHB) was achieved by using recombinant Escherichia coli cells expressing separately or co-expressing a mutant halohydrin dehalogenase gene from Agrobacterium radiobacter AD1 and a nitrilase gene from Arabidopsis thaliana. The activity of nitrilase was inhibited by high concentration of ECHB and NaCN. Consequently, the one-pot one-step process was implemented by fed-batch of ECHB and NaCN with high accumulative product concentration (up to 0.9molL(-1)). The biotransformation of ECHB to EHG was successfully achieved at 1.2molL(-1) substrate concentration by a one-pot two-step process. As such, this one-pot bienzymatic transformation should be useful in synthesizing these important optical pure -hydroxycarboxylic acids.
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