Improvement of antioxidant activity and active ingredient of Dendrobium officinale via microbial fermentation

This study used brewer's yeast to ferment Dendrobium officinale and single-factor and orthogonal experiments were conducted to determine the optimal fermentation conditions. The antioxidant capacity of Dendrobium fermentation solution was also investigated by in vitro experiments, which showed that different concentrations of fermentation solution could effectively enhance the total antioxidant capacity of cells. The fermentation liquid was found to contain seven sugar compounds including glucose, galactose, rhamnose, arabinose, and xylose using gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography-quadrupole-time of flight mass spectrometry (HPLC-Q-TOF-MS), with the highest concentrations of glucose and galactose at 194.628 and 103.899 mu g/ml, respectively. The external fermentation liquid also contained six flavonoids with apigenin glycosides as the main structure and four phenolic acids including gallic acid, protocatechuic acid, catechol, and sessile pentosidine B.

Automated summary

This study used brewer's yeast to ferment Dendrobium officinale and determined the optimal fermentation conditions through single-factor and orthogonal experiments. In vitro experiments showed that different concentrations of the fermentation solution effectively enhanced the total antioxidant capacity of cells. Analysis using gas chromatography-mass spectrometry and high performance liquid chromatography-quadrupole-time of flight mass spectrometry revealed the presence of seven sugar compounds and six flavonoids in the fermentation liquid.