Article
Chemistry, Analytical
Yixing Gou, Jiawen Liu, Changku Sun, Peng Wang, Zheng You, Dahai Ren
Summary: This paper presents a method for isolating rare CTCs from cell bundles using self-assembled immunomagnetic beads under the assistance of the spiral inertial effect, providing an important technical tool for cancer research. Through parametric numerical simulations, the velocity distribution in the cross section was explored, enabling the recovery of rare CTCs at a throughput of 500 μL/min on this chip, making it a valuable supplement in cancer analysis, diagnostics, and therapeutics.
Article
Chemistry, Multidisciplinary
Juan Li, Yu Xia, Fuling Zhou, Rongxiang He, Bolei Chen, Shishang Guo
Summary: In this work, a smart device integrating cell capture, in situ manipulation, and non-destructive release was designed to efficiently and rapidly capture cancer cells and deliver drugs. It has significant applications in medical treatment, tumor research, and drug development.
Article
Chemistry, Analytical
Chanyong Park, Abdurhaman Teyib Abafogi, Dinesh Veeran Ponnuvelu, Ilchan Song, Kisung Ko, Sungsu Park
Summary: This study focuses on improving the ATP luminescence assay for the detection of CTCs by using a 3D printed immunomagnetic concentrator (3DPIC) to separate and concentrate CTCs in the blood. The results show that 3DPIC can concentrate cancer cells in blood by 100 times within minutes, enabling the ATP luminescence assay to detect as low as 10 cells in blood, which is approximately 10 times more sensitive than using commercial kits for CTC concentration.
Article
Nanoscience & Nanotechnology
Yi Zhang, Wenjing Wang, Huiling Guo, Mingxing Liu, Hongda Zhu, Hongmei Sun
Summary: The development of a hyaluronic acid-functionalized redox responsive immunomagnetic nanocarrier for efficient capture and release of tumor cells holds promise for targeted cancer treatment. The nanocarrier demonstrated high capture efficiency and viability maintenance of released tumor cells, suggesting its potential as a new weapon against CD44 receptor-overexpressed cancer cells.
Article
Nanoscience & Nanotechnology
Yuwei Zhou, Xiaoshan Wang, Zhouying Luo, Xia Liu, Jianwen Hou, Shaobing Zhou
Summary: A novel multifunctional platform based on fluorescent-magnetic nanoparticles was developed for efficient isolation of circulating tumor cells (CTCs). The platform captured over 88% of EpCAM-positive and EpCAM-negative cancer cells, and released the captured cells with high efficiency and viabilities. It also exhibited excellent resistance to nonspecific adhesion of white blood cells and high detection sensitivity toward cancer cells in the patients' blood. This platform offers a good solution to address the current challenges of CTCs isolation.
Article
Biochemistry & Molecular Biology
Gabor Jarvas, Dora Szerenyi, Jozsef Tovari, Laszlo Takacs, Andras Guttman
Summary: The study successfully achieved selective capture of circulating tumor cells by modifying hemodialysis membranes. The technology effectively removed tumor cells without affecting the porous structure of the membranes, while also addressing cachexia simultaneously.
Article
Materials Science, Multidisciplinary
Guohao Li, Nanhang Zhu, Jia Cheng, Yujia Zhang, Yue Yu, Xiaolin Zhang, Qiangying Yi, Yao Wu
Summary: In this study, Fr-IMBs were successfully fabricated for efficient capture of MCF-7 exosomes, with a high release yield of competitively bound exosomes after fructose triggered release. The enriched exosomes retained their bioactivity and showed higher purity and better preservation of tumor-related molecular information compared to ultracentrifugation.
MATERIALS & DESIGN
(2022)
Article
Multidisciplinary Sciences
Chia-Heng Chu, Ruxiu Liu, Tevhide Ozkaya-Ahmadov, Brandi E. Swain, Mert Boya, Bassel El-Rayes, Mehmet Akce, Mehmet Asim Bilen, Omer Kucuk, A. Fatih Sarioglu
Summary: The device efficiently enriches viable CTCs through negative enrichment and isolates them from clinical samples while effectively depleting white blood cells, demonstrating the potential for standardized blood-based tumor biopsies.
SCIENTIFIC REPORTS
(2021)
Article
Pharmacology & Pharmacy
Huan Deng, Xiangqin Liu, Jie Chen, Yi He, Lanke Lin, Xin Liu, Jiang Chen, Xiaoqi Liu
Summary: Efficiently capturing circulating tumor cells (CTCs) in blood is crucial for cancer screening, treatment, and risk assessment of metastasis or recurrence. Current methods for capturing CTCs often require multiple chemical reagents and a complex pre-treatment process. In this study, a simple strategy was developed using photo-functionalized titanium dioxide (TiO2) to immobilize EpCAM antibodies without additional chemical reagents. The positive charge of the TiO2 surface allowed for electrostatic interaction and effective immobilization of EpCAM antibodies, leading to efficient CTC capture both in vitro and in vivo. This strategy shows promise for future applications in CTC capture.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Chemistry, Analytical
Abdallah M. Alkhaiyat, Mohamed Badran
Summary: This study proposes a lab-on-chip design that efficiently separates circulating tumor cells (CTCs) from white blood cells (WBCs) and blood platelets (PLTs). Numerical simulations are used to optimize the design parameters, and the effects of applied voltage values and buffer inlet velocity are investigated. The proposed design achieves 100% separation efficiency in simulation and shows potential for early detection of CTCs and personalized cancer treatment.
Review
Chemistry, Multidisciplinary
Hanyue Kang, Yuting Xiong, Liang Ma, Tongqing Yang, Xiaobin Xu
Summary: This review summarizes the recent advancements in micro/nanostructure array integrated microfluidic devices for the separation of circulating tumor cells (CTCs). The mechanisms by which these structures contribute to improved capture efficiency are discussed, along with the two major categories of separation methods based on the physical and biological properties of CTCs. These emerging technologies are expected to become powerful tools for disease diagnosis in the future.
Article
Chemistry, Multidisciplinary
Yue Yu, Yujia Zhang, Yu Chen, Xuekang Wang, Ke Kang, Nanhang Zhu, Yao Wu, Qiangying Yi
Summary: In this study, floating immunomagnetic microspheres (FIMMs) and their matching rotary magnetic manipulation device were used to achieve highly efficient capture of circulating tumor cells (CTCs) under a cell-friendly condition. The FIMMs were prepared through layer-by-layer assembly and their stress state controlled by the rotary magnetic manipulation device. With this system, satisfactory enrichment efficiencies were obtained for CTCs with varied epithelial expression levels and a low detection limit was achieved.
Article
Biophysics
Liang Zhu, Xingqing Feng, Shenhao Yang, Jianyun Wang, Yixin Pan, Jinhua Ding, Chenglin Li, Xiaoxing Yin, Yanyan Yu
Summary: This study developed a technique using modified IMNs and a colorimetric probe to capture and detect rare CTCs, achieving accurate quantification of CTCs in the blood of clinical tumor patients.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Chenglin Li, Rui Li, Xueyuan Wu, Yifan Zuo, Guixiang Xiong, Meng Huang, Yuqing Sun, Rong Liao, Yang Xiao, Lili Hu, Chao Gao, Yanyan Yu
Summary: This study systematically investigates the associations between the number and phenotypes of circulating tumor cells (CTCs) and the progression of colorectal cancer (CRC). A novel anti-nonspecific adsorption immunomagnetic platform was developed and demonstrated to efficiently capture CTCs of different phenotypes. The results showed that the proportion of M-CTCs is closely associated with the progression, diagnosis, surgery, and chemotherapeutic effects in CRC patients.
ANALYTICAL CHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Jiahao Dai, Bo Cai, Yan Zhang, Shukun Zhao, Xingzhong Zhao, Guobin Wang, Lin Wang, Zheng Wang
Summary: A gelatin-coated ZnO array nanoplatform is developed to isolate circulating tumor cells (CTCs) and assess their drug resistance, providing a facile, fast, and noninvasive method for evaluating tumor sensitivity to drugs.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Chemistry, Analytical
Yi-Yan Bai, Zhi-Tao Feng, Yan-Ju Yang, Xiao-Yan Yang, Zhi-Ling Zhang
Summary: This study utilized current lifetime as a dynamic parameter to distinguish differently sized nanoparticles successfully. By considering current lifetime instead of current intensity, more accurate evaluation of nanoparticle sizes can be achieved.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Ji Wang, Hao-Tian Ma, Liang-Jun Pan, Li Zhang, Zhi-Ling Zhang
Summary: Aqueous phase synthesized ternary I-III-VI2 Quantum dots (QDs) have gained increasing attention in biology research due to their good biocompatibility and easily adjustable fluorescence properties. However, the low quantum yield (QY) of these QDs synthesized in aqueous phase hinders their further applications. In this study, an integrated droplet microfluidic chip was proposed for the automated and successive synthesis and ripening of AgInS2 QDs, achieving high-performance QDs with independently controlled and precise temperatures. The study also explored the reason for the decrease in QY of AgInS2 QDs at higher ripening temperature.
CHINESE CHEMICAL LETTERS
(2022)
Article
Virology
Bo Tang, En-Ze Sun, Zhi-Ling Zhang, Shu-Lin Liu, Jia Liu, Akihiro Kusumi, Zhi-Hong Hu, Tao Zeng, Ya-Feng Kang, Hong-Wu Tang, Dai-Wen Pang
Summary: In this study, researchers investigated the cellular endocytic machineries used by influenza A virus (IAV) and found that sphingomyelin (SM)-sequestered cholesterol plays a crucial role in the clathrin-mediated endocytosis (CME) of IAV. They also discovered that the SM-cholesterol complex helps in the constriction of the clathrin-coated structure (CCS) containing IAV. These findings provide new insights into the mechanisms of IAV entry and highlight the importance of the SM-cholesterol complex.
JOURNAL OF VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Jing-Ya Zhao, Zhi-Gang Wang, Hui Hu, Zhi-Ling Zhang, Bo Tang, Meng-Yao Luo, Ling-Ling Yang, Baoshan Wang, Dai-Wen Pang
Summary: The surface chemistry of nanocrystals, such as Ag2Se quantum dots, can be controlled and tuned by adjusting their size or composition, which affects their photoluminescence quantum yield and photostability.
Article
Chemistry, Analytical
Jing Liu, Zi-Li Yu, Rui Xu, Zhi-Ling Zhang, Xuechuan Hong, Shifeng Wang, Gang Chen, Zhi-Quan Tian
Summary: Studies have shown that sEV PD-L1 levels can predict the clinical responses to immunotherapy. However, current quantitative analysis methods for sEV PD-L1 are time-consuming and have low sensitivity. In this study, a FSAP probe was developed for sensitive and rapid detection of sEV PD-L1, overcoming the interference of free forms of PD-L1. The FSAP method showed high sensitivity and could detect PD-L1-positive sEVs in diluted plasma samples, distinguishing between cancer patients and healthy donors.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Multidisciplinary Sciences
Biao Huang, Tao Tang, Shi-Hui Chen, Hao Li, Zhi-Jun Sun, Zhi-Lin Zhang, Mingxi Zhang, Ran Cui
Summary: The authors designed a near-infrared-IIb emitting Mn single-atom catalyst for imaging-guided therapy to alleviate ROS-mediated neuroinflammation and obtain feedback of therapeutic effect in mice with traumatic brain injury. The catalyst promoted the reconstruction of blood-brain barrier and recovery of neurological function.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Hou-Fu Xia, Zi-Li Yu, Li-Juan Zhang, Shu-Lin Liu, Yi Zhao, Jue Huang, Dan-Dan Fu, Qi-Hui Xie, Hai-Ming Liu, Zhi-Ling Zhang, Yi-Fang Zhao, Min Wu, Wei Zhang, Dai-Wen Pang, Gang Chen
Summary: This study proposes a single particle tracking platform using a quantum dot labeling strategy to dissect the dynamic behaviors of extracellular vesicles (EVs). The interplay between tumor cell-derived small EVs (T-sEVs) and endothelial cells (ECs) is investigated, revealing the entire process and detailed dynamics of T-sEV transportation and cargo-release in ECs. This study provides insights into the proangiogenic functions of T-sEVs and highlights the potential of the QD-based single particle tracking technique in exploring sEV-mediated cell-cell communication.
Article
Chemistry, Analytical
Yi-Yan Bai, Yan-Ju Yang, Ying Xu, Xiao-Yan Yang, Zhi-Ling Zhang
Summary: A current lifetime-based single-nanoparticle electrochemical collision (SNEC) method was developed to distinguish a single Au NP (d = 18 nm) from its agglomeration/aggregation. Results showed that the agglomeration/aggregation of 18 nm Au NPs in 0.8 mM HClO4 increased from 19% to 69% over two hours. The proposed SNEC method can be used to monitor the agglomeration/aggregation of small-sized NPs at the single-particle level and provide guidance for their practical application.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jiao Feng, Bo-Lin Xiao, Lin-Zhou Zhang, Yi-Hua Zhang, Chun-Miao Xu, Man Tang, Gang Chen, Zhi-Ling Zhang
Summary: This article presents a simple microfluidic approach for the on-chip specific isolation and detection of two phenotypes of extracellular vesicles (EVs) based on different biomolecule-modified magnetic nanospheres and a fluorescence labeling technique. The method has been successfully applied to clinical saliva samples in oral squamous cell carcinoma (OSCC) patients and healthy individuals, showing that the total level of EGFR+ EVs was significantly higher in OSCC patients compared to healthy individuals. Moreover, the ratio of Annexin V+ EGFR+ EVs to Annexin V- EGFR+ EVs was found to be negatively correlated with the tumor T stage of OSCC patients, suggesting its potential as a clinical index for monitoring OSCC progression.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jiao Feng, Chun-Miao Xu, Hou-Fu Xia, Hai-Ming Liu, Man Tang, Yi-Shen Tian, Gang Chen, Zhi-Ling Zhang
Summary: Liquid biopsy technology involves minimally invasive sampling of body fluids to analyze tumor markers for early tumor diagnosis and efficacy evaluation. This study presents a 3D magnetic chip-based extracorporeal circulation system for in vivo detection and real-time monitoring of circulating tumor cells (CTCs). By utilizing biofunctionalized magnetic nanospheres (MNs) with CTC recognition function, this 3DMC system enables efficient real-time monitoring of CTCs with good stability and strong anti-interference. In vivo detection not only detects more CTCs compared to in vitro detection but also identifies CTCs at an early stage of tumor presence in the blood. With its flexible chip design, the system can easily integrate cancer diagnosis and treatment. The biocompatibility and stability of the 3DMC system make it a promising tool for personalized medical programs for cancer patients.
ANALYTICAL CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Kai Wang, Ku-Han Deng, Yi-Shen Tian, Meng-Ying Sun, Zi-li Yu, Zhi-Quan Tian, Zhi-Ling Zhang
Summary: A fluorescence material, Ag2Te/Ag2Se quantum dots, was developed for imaging in the near infrared IIb window. By coating the Ag2Te core with a Ag2Se shell, the fluorescence performance was significantly enhanced, allowing for high-resolution in vivo imaging.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Analytical
Jing Liu, Ming Chen, Zhi-Ling Zhang, Xuechuan Hong, Zi-Li Yu, Zhi-Quan Tian
Summary: Dopamine is a crucial neurotransmitter involved in the development of neurological diseases, and its accurate quantification is important for diagnosis. Current detection methods like electrochemical and colorimetric assays have limitations in sensitivity and selectivity. In this study, a biosensor called DFAP-SAB was developed, using NIR-II quantum dots and streptavidin to achieve rapid and separation-free detection of dopamine in human serum. The signal showed good linearity and a low detection limit. The use of NIR-II quantum dots and streptavidin signal amplification offer new possibilities for biosensor applications.
Article
Chemistry, Analytical
Yong-Tao Wang, Meng Wang, Ke-Zhu Yang, Zhi-Ling Zhang
Summary: A corresponding V-lattice structure was constructed in a microfluidic chip in order to enhance the binding process between targets and oligonucleotide libraries. Computational simulations demonstrated that the structure could enhance mass transfer in the channel, leading to the discovery of an aptamer with high affinity and specificity for the target protein. This work introduces a new research idea for improving selection pressure in SELEX and also has potential applications in detection, sensing, and other fields.
SENSORS & DIAGNOSTICS
(2023)
Article
Biochemical Research Methods
Yueyue Huangfu, Ji Wang, Jiao Feng, Zhi-Ling Zhang
Summary: This study constructs a distal renal tubular system-on-a-chip to investigate the pathogenesis of influenza virus-induced acute kidney injury. It reveals the pathways of virus entry into the kidney and the mechanisms of virus infection in renal tubules.
Article
Chemistry, Multidisciplinary
Man Tang, Jiao Feng, Hou-Fu Xia, Chun-Miao Xu, Ling-Ling Wu, Min Wu, Shao-Li Hong, Gang Chen, Zhi-Ling Zhang
Summary: This paper presents a continuous magnetic separation microfluidic chip that allows for rapid and continuous in vivo cell detection. The chip has shown potential in studying the circulation of tumor cells in the blood, providing a new platform for exploring the cellular mechanism of tumor metastasis.
CHEMICAL COMMUNICATIONS
(2023)