Polymerase chain reaction in microfluidic devices

Optimization of 3D Digital Microfluidic Biochips for the Multiplexed Polymerase Chain Reaction

(2016) Zipeng Li et al.   ACM TRANSACTIONS ON DESIGN AUTOMATION OF ELECTRONIC SYSTEMS

Development and application of absolute quantitative detection by duplex chamber-based digital PCR of genetically modified maize events without pretreatment steps

(2016) Pengyu Zhu et al.   ANALYTICA CHIMICA ACTA

Palm-Sized Device for Point-of-Care Ebola Detection

(2016) Christian D. Ahrberg et al.   ANALYTICAL CHEMISTRY

A BSA coated NOA81 PCR chip for gene amplification

(2016) Kuiwei Qin et al.   Analytical Methods

Digital droplet LAMP as a microfluidic app on standard laboratory devices

(2016) Friedrich Schuler et al.   Analytical Methods

An integrated microfluidic system for diagnosis of the resistance of Helicobacter pylori to quinolone-based antibiotics

(2016) Chih-Yu Chao et al.   BIOSENSORS & BIOELECTRONICS

On-chip quantitative PCR using integrated real-time detection by capillary electrophoresis

(2016) Yu Liu et al.   ELECTROPHORESIS

Droplet digital polymerase chain reaction detection of HER2 amplification in formalin fixed paraffin embedded breast and gastric carcinoma samples

(2016) Yazhen Zhu et al.   EXPERIMENTAL AND MOLECULAR PATHOLOGY

A direct droplet digital PCR method for quantification of residual DNA in protein drugs produced in yeast cells

(2016) Musaddeq Hussain et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Digital droplet PCR on disk

(2016) Friedrich Schuler et al.   LAB ON A CHIP

Handheld real-time PCR device

(2016) Christian D. Ahrberg et al.   LAB ON A CHIP

Ultrafast, sensitive and large-volume on-chip real-time PCR for the molecular diagnosis of bacterial and viral infections

(2016) Timothée Houssin et al.   LAB ON A CHIP

Multiplex Detection of Rare Mutations by Picoliter Droplet Based Digital PCR: Sensitivity and Specificity Considerations

(2016) Eleonora Zonta et al.   PLoS One

A microfluidic lab-on-a-disc integrated loop mediated isothermal amplification for foodborne pathogen detection

(2016) Abkar Ahmed Sayad et al.   SENSORS AND ACTUATORS B-CHEMICAL

Extensible Multiplex Real-time PCR of MicroRNA Using Microparticles

(2016) Seungwon Jung et al.   Scientific Reports

KS-Detect – Validation of Solar Thermal PCR for the Diagnosis of Kaposi’s Sarcoma Using Pseudo-Biopsy Samples

(2016) Ryan Snodgrass et al.   PLoS One

A Rapid and Low-Cost PCR Thermal Cycler for Infectious Disease Diagnostics

(2016) Kamfai Chan et al.   PLoS One

Integrated Electrochemical Microsystems for Genetic Detection of Pathogens at the Point of Care

(2015) Kuangwen Hsieh et al.   ACCOUNTS OF CHEMICAL RESEARCH

Detecting and Number Counting of Single Engineered Nanoparticles by Digital Particle Polymerase Chain Reaction

(2015) Daniela Paunescu et al.   ACS Nano

A disposable laser print-cut-laminate polyester microchip for multiplexed PCR via infra-red-mediated thermal control

(2015) Yiwen Ouyang et al.   ANALYTICA CHIMICA ACTA

Real-time cdPCR opens a window into events occurring in the first few PCR amplification cycles

(2015) David L. Duewer et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Isothermal circular-strand-displacement polymerization of DNA and microRNA in digital microfluidic devices

(2015) Maria Chiara Giuffrida et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

An integrated microfluidic system for bovine DNA purification and digital PCR detection

(2015) Qingchang Tian et al.   ANALYTICAL BIOCHEMISTRY

Hands-Off Preparation of Monodisperse Emulsion Droplets Using a Poly(dimethylsiloxane) Microfluidic Chip for Droplet Digital PCR

(2015) Hironari Tanaka et al.   ANALYTICAL CHEMISTRY

Multiplex Quantification of 12 European Union Authorized Genetically Modified Maize Lines with Droplet Digital Polymerase Chain Reaction

(2015) David Dobnik et al.   ANALYTICAL CHEMISTRY

A localized temporary negative pressure assisted microfluidic device for detecting keratin 19 in A549 lung carcinoma cells with digital PCR

(2015) Qingchang Tian et al.   Analytical Methods

Effect of bias voltage and temperature on lifetime of wireless neural interfaces with Al2O3 and parylene bilayer encapsulation

(2015) Xianzong Xie et al.   BIOMEDICAL MICRODEVICES

One-heater flow-through polymerase chain reaction device by heat pipes cooling

(2015) Jyh Jian Chen et al.   Biomicrofluidics

Single-cell PCR of genomic DNA enabled by automated single-cell printing for cell isolation

(2015) F. Stumpf et al.   BIOSENSORS & BIOELECTRONICS

Absolute quantification of lung cancer related microRNA by droplet digital PCR

(2015) Ping Wang et al.   BIOSENSORS & BIOELECTRONICS

A microfluidic droplet digital PCR for simultaneous detection of pathogenic Escherichia coli O157 and Listeria monocytogenes

(2015) Xiaojun Bian et al.   BIOSENSORS & BIOELECTRONICS

Research to Improve the Efficiency of Double Stereo PCR Microfluidic Chip by Passivating the Inner Surface of Steel Capillary with NOA61

(2015) Jian Wu et al.   CELL BIOCHEMISTRY AND BIOPHYSICS

Species identification and quantification in meat and meat products using droplet digital PCR (ddPCR)

(2015) C. Floren et al.   FOOD CHEMISTRY

Miniaturized polymerase chain reaction device for rapid identification of genetically modified organisms

(2015) Minh Luan Ha et al.   FOOD CONTROL

Direct Detection of Influenza A and B Viruses in Less Than 20 Minutes Using a Commercially Available Rapid PCR Assay

(2015) Matthew J. Binnicker et al.   JOURNAL OF CLINICAL MICROBIOLOGY

A fully sealed plastic chip for multiplex PCR and its application in bacteria identification

(2015) Youchun Xu et al.   LAB ON A CHIP

A solvent replenishment solution for managing evaporation of biochemical reactions in air-matrix digital microfluidics devices

(2015) Mais J. Jebrail et al.   LAB ON A CHIP

Microfluidic vapor-diffusion barrier for pressure reduction in fully closed PCR modules

(2015) G. Czilwik et al.   LAB ON A CHIP

Centrifugal step emulsification applied for absolute quantification of nucleic acids by digital droplet RPA

(2015) Friedrich Schuler et al.   LAB ON A CHIP

Rapid and fully automated bacterial pathogen detection on a centrifugal-microfluidic LabDisk using highly sensitive nested PCR with integrated sample preparation

(2015) G. Czilwik et al.   LAB ON A CHIP

Microfluidic continuous flow digital loop-mediated isothermal amplification (LAMP)

(2015) Tushar D. Rane et al.   LAB ON A CHIP

Lack of correlation between reaction speed and analytical sensitivity in isothermal amplification reveals the value of digital methods for optimization: validation using digital real-time RT-LAMP

(2015) Eugenia M. Khorosheva et al.   NUCLEIC ACIDS RESEARCH

Automated Forensic Animal Family Identification by Nested PCR and Melt Curve Analysis on an Off-the-Shelf Thermocycler Augmented with a Centrifugal Microfluidic Disk Segment

(2015) Mark Keller et al.   PLoS One

Centrifugal Microfluidic System for Nucleic Acid Amplification and Detection

(2015) Baogang Miao et al.   SENSORS

Self-propelled continuous-flow PCR in capillary-driven microfluidic device: Microfluidic behavior and DNA amplification

(2015) Hiroaki Tachibana et al.   SENSORS AND ACTUATORS B-CHEMICAL

Centrifugo-thermopneumatic fluid control for valving and aliquoting applied to multiplex real-time PCR on off-the-shelf centrifugal thermocycler

(2015) M. Keller et al.   RSC Advances

An integrated temporary negative pressure assisted microfluidic chip for DNA isolation and digital PCR detection

(2015) Qingchang Tian et al.   RSC Advances

A bead-based microfluidic approach to integrated single-cell gene expression analysis by quantitative RT-PCR

(2015) Hao Sun et al.   RSC Advances

Printing 2-Dimentional Droplet Array for Single-Cell Reverse Transcription Quantitative PCR Assay with a Microfluidic Robot

(2015) Ying Zhu et al.   Scientific Reports

Single Fluorescence Channel-based Multiplex Detection of Avian Influenza Virus by Quantitative PCR with Intercalating Dye

(2015) Christian D. Ahrberg et al.   Scientific Reports

Ultrafast photonic PCR

(2015) Jun Ho Son et al.   Light-Science & Applications

PCR-Activated Cell Sorting for Cultivation-Free Enrichment and Sequencing of Rare Microbes

(2015) Shaun W. Lim et al.   PLoS One

Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR

(2014) Adrian M. Schrell et al.   ANALYST

DNA Analysis Using an Integrated Microchip for Multiplex PCR Amplification and Electrophoresis for Reference Samples

(2014) Delphine Le Roux et al.   ANALYTICAL CHEMISTRY

Point-of-Care Multiplexed Assays of Nucleic Acids Using Microcapillary-based Loop-Mediated Isothermal Amplification

(2014) Yi Zhang et al.   ANALYTICAL CHEMISTRY

Self-Digitization Microfluidic Chip for Absolute Quantification of mRNA in Single Cells

(2014) Alison M. Thompson et al.   ANALYTICAL CHEMISTRY

Real-time PCR based detection of a panel of food-borne pathogens on a centrifugal microfluidic “LabDisk” with on-disk quality controls and standards for quantification

(2014) Oliver Strohmeier et al.   Analytical Methods

Quasi-digital PCR: Enrichment and quantification of rare DNA variants

(2014) Scott O. Sundberg et al.   BIOMEDICAL MICRODEVICES

A miniature quantitative PCR device for directly monitoring a sample processing on a microfluidic rapid DNA system

(2014) Cedric Hurth et al.   BIOMEDICAL MICRODEVICES

A real-time microfluidic multiplex electrochemical loop-mediated isothermal amplification chip for differentiating bacteria

(2014) Juan Luo et al.   BIOSENSORS & BIOELECTRONICS

Extreme PCR: Efficient and Specific DNA Amplification in 15-60 Seconds

(2014) J. S. Farrar et al.   CLINICAL CHEMISTRY

Microfluidic Genotyping by Rapid Serial PCR and High-Speed Melting Analysis

(2014) S. O. Sundberg et al.   CLINICAL CHEMISTRY

A multiplexed droplet digital PCR assay performs better than qPCR on inhibition prone samples

(2014) Ruth Hall Sedlak et al.   DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE

Development and evaluation of a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip LAMP) for rapid and simultaneous detection of ten pathogenic bacteria in aquatic animals

(2014) Qian-Jin Zhou et al.   JOURNAL OF MICROBIOLOGICAL METHODS

A microfluidic device providing continuous-flow polymerase chain reaction heating and cooling

(2014) A Harandi et al.   JOURNAL OF MICROMECHANICS AND MICROENGINEERING

Diffusion-based microfluidic PCR for “one-pot” analysis of cells

(2014) Sai Ma et al.   LAB ON A CHIP

An integrated microfluidic device utilizing dielectrophoresis and multiplex array PCR for point-of-care detection of pathogens

(2014) Dongyang Cai et al.   LAB ON A CHIP

An integrated, cellulose membrane-based PCR chamber

(2014) Xianbo Qiu et al.   MICROSYSTEM TECHNOLOGIES-MICRO-AND NANOSYSTEMS-INFORMATION STORAGE AND PROCESSING SYSTEMS

All-plastic, low-power, disposable, continuous-flow PCR chip with integrated microheaters for rapid DNA amplification

(2014) Despina Moschou et al.   SENSORS AND ACTUATORS B-CHEMICAL

Solar thermal polymerase chain reaction for smartphone-assisted molecular diagnostics

(2014) Li Jiang et al.   Scientific Reports

Multiplex, Quantitative, Reverse Transcription PCR Detection of Influenza Viruses Using Droplet Microfluidic Technology

(2014) Ravi Prakash et al.   Micromachines

Mechanistic Evaluation of the Pros and Cons of Digital RT-LAMP for HIV-1 Viral Load Quantification on a Microfluidic Device and Improved Efficiency via a Two-Step Digital Protocol

(2013) Bing Sun et al.   ANALYTICAL CHEMISTRY

Increased Robustness of Single-Molecule Counting with Microfluidics, Digital Isothermal Amplification, and a Mobile Phone versus Real-Time Kinetic Measurements

(2013) David A. Selck et al.   ANALYTICAL CHEMISTRY

Simultaneous Identification of Pork and Poultry Origins in Pet Foods by a Quick Multiplex Real-Time PCR Assay Using EvaGreen Florescence Dye

(2013) M. Safdar et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Polymerase chain reaction with phase change as intrinsic thermal control

(2013) Yi-Fan Hsieh et al.   APPLIED PHYSICS LETTERS

Development and validation of a multiplex quantitative PCR assay for the rapid detection of Grapevine virus A, B and D

(2013) Fatima Osman et al.   JOURNAL OF VIROLOGICAL METHODS

A microfluidic chip integrating DNA extraction and real-time PCR for the detection of bacteria in saliva

(2013) Emily A. Oblath et al.   LAB ON A CHIP

Multiplex genotyping of KRAS point mutations in tumor cell DNA by allele-specific real-time PCR on a centrifugal microfluidic disk segment

(2013) Oliver Strohmeier et al.   MICROCHIMICA ACTA

Evaluation of Digital PCR for Absolute RNA Quantification

(2013) Rebecca Sanders et al.   PLoS One

A Simple and Efficient Multiplex PCR Assay for the Identification ofMycobacteriumGenus andMycobacterium tuberculosisComplex to the Species Level

(2013) Yeun Kim et al.   YONSEI MEDICAL JOURNAL

Detection of expressed gene in isolated single cells in microchambers by a novel hot cell-direct RT-PCR method

(2012) Shunsuke Furutani et al.   ANALYST

Digital Polymerase Chain Reaction in an Array of Femtoliter Polydimethylsiloxane Microreactors

(2012) Yongfan Men et al.   ANALYTICAL CHEMISTRY

Plug-and-play, infrared, laser-mediated PCR in a microfluidic chip

(2012) Nikita Pak et al.   BIOMEDICAL MICRODEVICES

A microfluidic device for on-chip agarose microbead generation with ultralow reagent consumption

(2012) Linda Desbois et al.   Biomicrofluidics

Nanofluidic Digital PCR for KRAS Mutation Detection and Quantification in Gastrointestinal Cancer

(2012) D. Azuara et al.   CLINICAL CHEMISTRY

Discriminatory simplex and multiplex PCR for four species of the genus Sclerotinia

(2012) Ahmed Abd-Elmagid et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns

(2012) Veronica Sanchez-Freire et al.   Nature Protocols

Detect early stage lung cancer by a LAMP microfluidic chip system with a real-time fluorescent filter processor

(2012) TongZhou Wang et al.   Science China-Chemistry

Molecular quantification of environmental DNA using microfluidics and digital PCR

(2012) Tatsuhiko Hoshino et al.   SYSTEMATIC AND APPLIED MICROBIOLOGY

Detection of toxigenic Vibrio cholerae with new multiplex PCR

(2012) Jalil Fallah Mehrabadi et al.   Journal of Infection and Public Health

Under-three minute PCR: Probing the limits of fast amplification

(2011) E. K. Wheeler et al.   ANALYST

Three-dimensional on-chip continuous-flow polymerase chain reaction employing a single heater

(2011) Wenming Wu et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

VereFlu™: an integrated multiplex RT-PCR and microarray assay for rapid detection and identification of human influenza A and B viruses using lab-on-chip technology

(2011) Jeanette Teo et al.   ARCHIVES OF VIROLOGY

Multichannel oscillatory-flow multiplex PCR microfluidics for high-throughput and fast detection of foodborne bacterial pathogens

(2011) Chunsun Zhang et al.   BIOMEDICAL MICRODEVICES

Single cell transcriptional profiling reveals heterogeneity of human induced pluripotent stem cells

(2011) Kazim H. Narsinh et al.   JOURNAL OF CLINICAL INVESTIGATION

A lab-on-a-chip device for rapid identification of avian influenza viral RNA by solid-phase PCR

(2011) Yi Sun et al.   LAB ON A CHIP

Integrated DNA purification, PCR, sample cleanup, and capillary electrophoresis microchip for forensic human identification

(2011) Peng Liu et al.   LAB ON A CHIP

Multiplex digital PCR: breaking the one target per color barrier of quantitative PCR

(2011) Qun Zhong et al.   LAB ON A CHIP

A disposable, integrated loop-mediated isothermal amplification cassette with thermally actuated valves

(2011) Changchun Liu et al.   Microfluidics and Nanofluidics

Megapixel digital PCR

(2011) Kevin A Heyries et al.   NATURE METHODS

FilmArray, an Automated Nested Multiplex PCR System for Multi-Pathogen Detection: Development and Application to Respiratory Tract Infection

(2011) Mark A. Poritz et al.   PLoS One

High-throughput microfluidic single-cell RT-qPCR

(2011) A. K. White et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Centrifugal microfluidic platform for rapid PCR amplification using integrated thermoelectric heating and ice-valving

(2011) Mary Amasia et al.   SENSORS AND ACTUATORS B-CHEMICAL

Compact disk (CD)-shaped device for single cell isolation and PCR of a specific gene in the isolated cell

(2010) Shunsuke Furutani et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Predicting Viruses Accurately by a Multiplex Microfluidic Loop-Mediated Isothermal Amplification Chip

(2010) Xueen Fang et al.   ANALYTICAL CHEMISTRY

Rapid nested-PCR for tyrosinase gene detection on chip

(2010) Anna Giovanna Sciancalepore et al.   BIOSENSORS & BIOELECTRONICS

An integrated microfluidic loop-mediated-isothermal-amplification system for rapid sample pre-treatment and detection of viruses

(2010) Chih-Hung Wang et al.   BIOSENSORS & BIOELECTRONICS

Agarose droplet microfluidics for highly parallel and efficient single molecule emulsion PCR

(2010) Xuefei Leng et al.   LAB ON A CHIP

Fast identification of foodborne pathogenic viruses using continuous-flow reverse transcription-PCR with fluorescence detection

(2010) Yuyuan Li et al.   Microfluidics and Nanofluidics

On-chip PCR amplification of genomic and viral templates in unprocessed whole blood

(2010) Dammika P. Manage et al.   Microfluidics and Nanofluidics

SlipChip

(2009) Wenbin Du et al.   LAB ON A CHIP

Petri dish PCR: laser-heated reactions in nanoliter droplet arrays

(2009) Hanyoup Kim et al.   LAB ON A CHIP

Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets

(2008) Yolanda Schaerli et al.   ANALYTICAL CHEMISTRY

Whole genome amplification on poly(dimethylsiloxane) microchip array

(2007) Lin Chen et al.   ANALYTICAL BIOCHEMISTRY