Article
Biochemical Research Methods
Ruben Van den Eeckhoudt, An-Sofie Christiaens, Frederik Ceyssens, Vasileios Vangalis, Kevin J. Verstrepen, Nico Boon, Filip Tavernier, Michael Kraft, Irene Taurino
Summary: This paper introduces a microfluidic device for full-electric cell capturing, analyzing, and selectively releasing single cells. The device was experimentally demonstrated on Saccharomyces cerevisiae, showing potential for small-scale, high-throughput single-cell analysis and sorting.
Article
Biochemical Research Methods
Ruben van den Eeckhoudt, An-Sofie Christiaens, Frederik Ceyssens, Vasileios Vangalis, Kevin J. Verstrepen, Nico Boon, Filip Tavernier, Michael Kraft, Irene Taurino
Summary: This paper presents a microfluidic device that combines a method for capturing, analyzing, and selectively releasing single cells with full-electricity. The device validates a new Two-Voltage method for capturing single cells by positive dielectrophoresis (pDEP) and utilizes broadband electrochemical impedance spectroscopy for cell analysis. The device also allows for selective release of trapped cells by turning off the pDEP signal in their trap.
Article
Chemistry, Analytical
Jonas M. Nikoloff, Mario A. Saucedo-Espinosa, Petra S. Dittrich
Summary: By analyzing the protein profiles of single extracellular vesicles (EVs), we found that EVs released by a single cell can represent its cell of origin. The microfluidic platform we used allowed for immobilization of EVs from individual cells, reducing the heterogeneity seen in cell populations. Through immunostaining and fluorescence microscopy, we determined the biochemical characteristics of membrane and cytosolic proteins in EVs. Our study demonstrates the potential of EVs as diagnostic markers and provides a method for studying EV biogenesis.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Eliet H. Sipos, Adelaide Lety-Stefanska, Cyril Denby Wilkes, Julie Soutourina, Florent Malloggi
Summary: The study developed a microfluidic system that automates yeast cell cultivation for mutation accumulation, reducing the time required for measuring the entire genome mutational profile. By performing experiments in different genetic backgrounds, it was shown that the microfluidic device allows for mutation accumulation comparable to traditional methods.
Article
Chemistry, Analytical
Enrique Azuaje-Hualde, Marian M. de Pancorbo, Fernando Benito-Lopez, Lourdes Basabe-Desmonts
Summary: This study proposes a low-cost and user-friendly paper microfluidic device for the detection of cell secreted VEGF. The device integrates a fluorescent VEGF biosensor into cellulose paper, enabling the detection of VEGF at low concentrations. It has the potential to be used in cell biology research, specifically for the detection of VEGF in the supernatant of mesenchymal stem cells culture plates.
ANALYTICA CHIMICA ACTA
(2022)
Article
Multidisciplinary Sciences
Jeremy A. Lombardo, Marzieh Aliaghaei, Quy H. Nguyen, Kai Kessenbrock, Jered B. Haun
Summary: The authors introduce a microfluidic platform combining tissue digestion, disaggregation, and filtration to enable single cell analysis and RNA sequencing. The platform generates more single cells for kidney and mammary tumor, enriching endothelial cells, fibroblasts, and basal epithelium. Processing time is significantly reduced for liver and heart, and periodic cell recovery during processing increases cell numbers and reproducibility from batch-to-batch.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Bader Al Alwan, Karmen AbuZineh, Shuho Nozue, Aigerim Rakhmatulina, Mansour Aldehaiman, Asma S. Al-Amoodi, Maged F. Serag, Fajr A. Aleisa, Jasmeen S. Merzaban, Satoshi Habuchi
Summary: By utilizing microfluidics and single-molecule live cell fluorescence imaging, this study demonstrates the crucial role of unique spatiotemporal dynamics of selectin ligands on membrane tethers and slings in the rolling behavior of cells.
COMMUNICATIONS BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Ana Mota, Szymon Berezicki, Erik Wernersson, Luuk Harbers, Xiaoze Li-Wang, Katarina Gradin, Christiane Peuckert, Nicola Crosetto, Magda Bienko
Summary: The authors of this study developed a method called FRET-FISH for probing chromatin compaction at specific loci in single cells. They validated the technique by comparing it with ATAC-seq and demonstrated its ability to detect changes in chromatin compaction under different conditions.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Qingyu Ruan, Jian Yang, Fenxiang Zou, Xiaofeng Chen, Qianqian Zhang, Kaifeng Zhao, Xiaoye Lin, Xi Zeng, Xiyuan Yu, Lingling Wu, Shuichao Lin, Zhi Zhu, Chaoyong Yang
Summary: This study introduces a single-CTC mass spectrometry analysis method based on digital microfluidics, which can efficiently and accurately perform multiplex mutation profiling of individual CTCs, offering new avenues for cancer therapy guidance.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Maxime Mistretta, Nicolas Gangneux, Giulia Manina
Summary: Preclinical analysis of drug efficacy is crucial for drug development. Conventional bulk-cell assays lack the ability to accurately assess drug efficacy on individual cells, leading to potential overestimation or rejection of valuable candidates. This study introduces a scalable microfluidic platform based on pneumatic operation, allowing for long-term live-cell imaging and analysis of different drug concentrations simultaneously. Experimental results on mycobacterial cells, including the tubercular pathogen, demonstrate the feasibility of the single-cell dose-response assay. This dynamic in-vitro model provides improved predictions of drug efficacy in clinical settings.
SCIENTIFIC REPORTS
(2022)
Article
Biology
Kevin Ziyang Chng, Yan Cheng Ng, Bumseok Namgung, Justin Kok Soon Tan, Soyeon Park, Sim Leng Tien, Hwa Liang Leo, Sangho Kim
Summary: The study using a microfluidic platform revealed that biologically older red blood cells have a faster oxygen release rate than younger cells, and blood storage enhances this release rate.
COMMUNICATIONS BIOLOGY
(2021)
Article
Chemistry, Analytical
Suhuur Osman, Claus Bendtsen, Samantha Peel, Linda Yrlid, Daniel Muthas, John Simpson, Keith R. Willison, David R. Klug
Summary: The cellular thermal shift assay (CETSA) is widely used to study drug-target engagement, especially in early stage drug discovery. Single-cell CETSA can accurately count active FOXO1 molecules and observe its stability and destabilization in the presence of small molecule inhibitors. The successful use of the MAC chip for single-cell CETSA enables the study of precious clinical samples and underlying population heterogeneity in cellular systems.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Dan Sun, Fanghao Cao, Xuan Yi, Hongyan Zhu, Guohua Qi, Weiqing Xu, Shuping Xu
Summary: Analysis of single-cell microRNA is essential for revealing genetic heterogeneity at the cell level. In this study, SERS and fluorescence imaging technology were used to achieve direct in situ, nondestructive, and highly sensitive detection of a small number of microRNA-21 (miR-21) in a single intact living cell. A multifunctional plasmonic nanoprobe was designed, allowing for highly sensitive and reliable detection of miR-21 through dual-signal switching. The combination of SERS and microdroplets encapsulation provided a feasible way to determine miR-21 secreted by single cells without cell lysis and time-consuming isolation steps. The research accurately and quantitatively evaluated the cellular heterogeneity in miR-21 expression, providing important reference information for understanding the effects of miRNAs on cancer diseases at the single-cell level.
Review
Chemistry, Multidisciplinary
Kevin S. Zhang, Ambika Nadkarni, Rajorshi Paul, Adrian M. Martin, Sindy K. Y. Tang
Summary: Microscale surgery has made significant advances in fundamental biology and engineering biological systems, but manual operations are labor-intensive and lack reproducibility. Microfluidics provides a powerful technology to control and manipulate cells and multicellular systems at the micro- and nanoscale with high precision. This review summarizes the physical and chemical mechanisms, design principles, applications, and implementations of microscale surgery in microfluidic systems. Existing challenges and opportunities are highlighted throughout the review.
Article
Multidisciplinary Sciences
Joachim De Jonghe, Tomasz S. Kaminski, David B. Morse, Marcin Tabaka, Anna L. Ellermann, Timo N. Kohler, Gianluca Amadei, Charlotte E. Handford, Gregory M. Findlay, Magdalena Zernicka-Goetz, Sarah A. Teichmann, Florian Hollfelder
Summary: The authors developed spinDrop, a technique that increases gene detection and reduces noise in droplet microfluidics, to generate a high-quality molecular atlas of mouse brain development.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Vladimir Paunovic, Sharon Mitchell, Rene Verel, Sung Sik Lee, Javier Perez-Ramirez
JOURNAL OF PHYSICAL CHEMISTRY C
(2020)
Article
Biochemical Research Methods
Jae Bem You, Byungjin Lee, Yunho Choi, Chang-Soo Lee, Matthias Peter, Sung Gap Im, Sung Sik Lee
Editorial Material
Chemistry, Analytical
Sung Sik Lee
Article
Cell Biology
Frank Van Drogen, Nicolas Dard, Serge Pelet, Sung Sik Lee, Ranjan Mishra, Nevena Srejic, Matthias Peter
Article
Multidisciplinary Sciences
Mart-Erik Maeots, Byungjin Lee, Andrea Nans, Seung-Geun Jeong, Mohammad M. N. Esfahani, Shan Ding, Daniel J. Smith, Chang-Soo Lee, Sung Sik Lee, Matthias Peter, Radoslav Enchev
NATURE COMMUNICATIONS
(2020)
Article
Biochemical Research Methods
Bookun Kim, Sung Sik Lee, Tae Hyeon Yoo, Ju Min Kim
Summary: This study found that under pressure-driven microflows, particles in human saliva and blood plasma are influenced by their viscoelastic properties, forming a focused stream. The degree of particle focusing varies among samples, indicating that the viscoelasticity of biofluids may be affected by their compositions.
Article
Environmental Sciences
Marlene Schwarzfischer, Anna Niechcial, Sung Sik Lee, Brian Sinnet, Marcin Wawrzyniak, Andrea Laimbacher, Kirstin Atrott, Roberto Manzini, Yasser Morsy, Janine Haefliger, Silvia Lang, Gerhard Rogler, Ralf Kaegi, Michael Scharl, Marianne R. Spalinger
Summary: Plastic pollution is a significant global challenge that poses a threat to the environment and human health. Ingested plastic particles, such as nanoplastic and microplastic, were found to have no promoting effects on inflammatory bowel disease (IBD), but potential accumulative effects on gastrointestinal health cannot be ruled out.
Article
Chemistry, Multidisciplinary
Julian T. C. Wennmacher, Soheil Mahmoudi, Przemyslaw Rzepka, Sung Sik Lee, Tim Gruene, Vladimir Paunovic, Jeroen A. van Bokhoven
Summary: This study presents the direct mapping of coke in the micropores of ZSM-5 catalysts using single-crystal electron diffraction analysis. The findings reveal that coke formation is associated with catalyst deactivation and provide a new method for analyzing coke deposition in the micropore domain.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Physical
Vladimir Paunovic, Vitaly Sushkevich, Przemyslaw Rzepka, Luca Artiglia, Roland Hauert, Sung Sik Lee, Jeroen A. van Bokhoven
Summary: This study investigates the potential of regenerating catalysts by reacting coke deposits with hydrogen. The reactivation efficiency is enhanced under high pressure, and hydrogen treatment can remove coke and restore catalyst activity in various Si/Al ratios.
JOURNAL OF CATALYSIS
(2022)
Article
Multidisciplinary Sciences
Vera M. Kissling, Giordano Reginato, Eliana Bianco, Kristina Kasaciunaite, Janny Tilma, Gea Cereghetti, Natalie Schindler, Sung Sik Lee, Raphael Guerois, Brian Luke, Ralf Seidel, Petr Cejka, Matthias Peter
Summary: This study reveals the oligomerization mechanism of the Mre11-Rad50 complex and its functional significance in DNA damage repair. The results show that Rad50 mediates the oligomerization of the Mre11-Rad50 complex, and its mutations may be linked to hereditary cancer predisposition.
NATURE COMMUNICATIONS
(2022)
Article
Biology
Anne C. Meinema, Anna Marzelliusardottir, Mihailo Mirkovic, Theo Aspert, Sung Sik Lee, Gilles Charvin, Yves Barral
Summary: The attachment of DNA circles to NPCs causes NPCs to lose their nuclear and cytoplasmic complexes in replicatively ageing yeast cells. This process is related to the replicative lifespan of the cells.
Article
Chemistry, Multidisciplinary
Yang Jun Kang, Sami Serhrouchni, Asya Makhro, Anna Bogdanova, Sung Sik Lee
Summary: Assessing the deformability of red blood cells (RBCs) as a biomarker usually requires expensive equipment. This study presents a simple method for quantifying RBC deformability using a microfluidic device. The temporal variation in blood pressure allowed the definition of a clogging index (CI) as a new marker of RBC deformability. Experimental results showed that the CI method provides reliable information on RBC deformability, comparable to traditional methods such as ektacytometry.
Article
Multidisciplinary Sciences
Maximilian Mosbacher, Sung Sik Lee, Gilad Yaakov, Mariona Nadal-Ribelles, Eulalia de Nadal, Frank van Drogen, Francesc Posas, Matthias Peter, Manfred Claassen
Summary: This study characterizes the regulation of MAP kinase Hog1 in Saccharomyces cerevisiae and reveals a mixed phosphorylation mechanism regulated by a positive feedback loop. Simulations show that this mechanism is necessary for full sensitivity to stimuli and robustness to perturbations.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Sun Ok Hong, Ki-Su Park, Dong-Yeong Kim, Sung Sik Lee, Chang-Soo Lee, Ju Min Kim
Summary: The study discusses the challenge of mixing in microscale flows and proposes an efficient mixing scheme by combining inertio-elastic flow instability with a modified serpentine channel design. Results show that this new scheme achieves highly efficient mixing over a wider range of flow rates and is successfully applied to the continuous synthesis of silica nanoparticles, resulting in nanoparticles with more uniform size distribution.
Article
Biochemical Research Methods
Byungjin Lee, Seong-Geun Jeong, Si Hyung Jin, Ranjan Mishra, Matthias Peter, Chang-Soo Lee, Sung Sik Lee