Journal
LAB ON A CHIP
Volume 16, Issue 7, Pages 1189-1196Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6lc00110f
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Funding
- National Leading Research Laboratory Program [NRF-2013R1A2A1A05006378]
- Bio & Medical Technology Development Program [NRF-2015M3A9B3028685]
- Converging Research Center Program through the National Research Foundation of Korea - Ministry of Science, ICT and Future Planning [2011K000864]
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We report a novel optoelectrofluidic immunoreaction system based on electroosmotic flow for enhancing antibody-analyte binding efficiency on a surface-based sensing system. Two conventional indium tin oxide glass slides are assembled to provide a reaction chamber for a tiny volume of sample droplet (similar to 5 mu L), in which the top layer is employed as an antibody-immobilized substrate and the bottom layer acts as a photoconductive layer of an optoelectrofluidic device. Under the application of an AC voltage, an illuminated light pattern on the photoconductive layer causes strong counter-rotating vortices to transport analytes from the bulk solution to the vicinity of the assay spot on the glass substrate. This configuration overcomes the slow immunoreaction problem of a diffusion-based sensing system, resulting in the enhancement of binding efficiency via an optoelectrofluidic method. Furthermore, we investigate the effect of optically-induced dynamic AC electroosmotic flow on optoelectrofluidic enhancement for surface-based immunoreaction with a mathematical simulation study and real experiments using immunoglobulin G (IgG) and anti-IgG. As a result, dynamic light patterns provided better immunoreaction efficiency than static light patterns due to effective mass transport of the target analyte, resulting in an achievement of 2.18-fold enhancement under a growing circular light pattern compared to the passive mode.
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