Journal
CHEMBIOCHEM
Volume 17, Issue 2, Pages 141-145Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201500471
Keywords
Candida antarctia lipase A; enzyme catalysis; flap; interfacial activation; lid; protein engineering
Funding
- Swedish Research Council (VR) [621-2013-4653]
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A mobile region is proposed to be a flap that covers the active site of Candida antarctica lipase A. Removal of the mobile region retains the functional properties of the enzyme. Interestingly interfacial activation, required for the wild-type enzyme, was not observed for the truncated variant, although stability, activity, and stereoselectivity were very similar for the wild-type and variant enzymes. The variant followed classical Michaelis-Menten kinetics, unlike the wild type. Both gave the same relative specificity in the transacylation of a primary and a secondary alcohol in organic solvent. Furthermore, both showed the same enantioselectivity in transacylation of alcohols and the hydrolysis of alcohol esters, as well as in the hydrolysis of esters chiral at the acid part.
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