Article
Agriculture, Dairy & Animal Science
Salma Akter, A. K. M. Mostafa Anower, M. M. Kamal Hossain, Md. Abdul Alim, Nusrat Jahan, Anjuman Ara Bhuyan, Md. Salimullah, Jahangir Alam
Summary: This study aimed to investigate the genetic variants of kappa-casein and beta-lactoglobulin genes in native cattle. Genotyping analysis revealed three genotypes (AA, AB, and BB) for both genes, with the AA genotype being the most common for kappa-casein and the BB genotype being the most common for beta-lactoglobulin. The results indicated a high genetic diversity, but a deviation from Hardy-Weinberg equilibrium, suggesting the presence of selection pressure in the population.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Parasitology
Riam Sadooni, Hassan Rezanezhad, Kavos Solhjoo, Mohsen Kalantari, Behrad Pourmohammadi, Saiedeh Erfanian, Belal Armand, Masoud Esmi Jahromi
Summary: This study aimed to genotype Toxoplasma gondii strains from slaughtered goats in Jahrom, showing the presence of different genotypes including three major and mixed genotypes. The diaphragm was found to be the most disposed organ to infection, with type I being the most frequent genotype. These results can be useful for the control and prevention of toxoplasmosis.
ACTA PARASITOLOGICA
(2022)
Article
Microbiology
Mercedes Fernandez-Escobar, Federica Giorda, Virgina Mattioda, Tania Audino, Fabio Di Nocera, Giuseppe Lucifora, Katia Varello, Carla Grattarola, Luis Miguel Ortega-Mora, Cristina Casalone, Rafael Calero-Bernal
Summary: This study investigated the genetic diversity of Toxoplasma gondii strains in stranded dolphins found in the Mediterranean coastlines of Italy. The results showed that dolphins were mainly infected with ToxoDB#3 and #2 genotypes.
Article
Genetics & Heredity
Nusrat Jahan, Mobashera Begum, Md Abdul Barek, Md. Abdul Aziz, Md. Shafiul Hossen, Khokon Kanti Bhowmik, Tahmina Akter, Md. Rabiul Islam, Hadi Sajid Abdulabbas, Mohammad Safiqul Islam
Summary: Breast cancer is the leading cause of cancer-related deaths in women. Variants of the FGFR2 gene have been found to be strongly associated with breast cancer risk. This study investigated the association between FGFR2 gene polymorphisms and breast cancer in a Bangladeshi population. The findings showed significant associations between certain FGFR2 variants (rs1219648 and rs2981582) and breast malignancy risk.
Article
Biochemistry & Molecular Biology
Eleanor C. Sheppard, Claudia A. Martin, Claire Armstrong, Catalina Gonzalez-Quevedo, Juan Carlos Illera, Alexander Suh, Lewis G. Spurgin, David S. Richardson
Summary: Understanding the mechanisms and genes that enable animals to adapt to pathogens is crucial. This study explores the genetic variation associated with avian pox infection in Berthelot's pipit populations. At the individual level, there was no evidence of variation in MHC class I and TLR4 being associated with pox infection. However, at the population-level, strong associations were found between avian pox prevalence and allele frequencies of SNPs located within genes involved in stress signalling and immune responses.
Article
Microbiology
Francesca Grippi, Vincenza Cannella, Giusi Macaluso, Valeria Blanda, Giovanni Emmolo, Francesco Santangelo, Domenico Vicari, Paola Galluzzo, Carmela Sciacca, Rosalia D'Agostino, Ilenia Giacchino, Cristina Bertasio, Mario D'Incau, Annalisa Guercio, Alessandra Torina
Summary: This study investigates the prevalence of Leptospira spp. in stray animals, finding higher infection rates in dogs compared to cats. Pathogenic Leptospira spp. were also detected in the urine and blood of stray cats.
Article
Entomology
Jian-Zhao Jiang, Bo-Yu Huang, Qian Wu, Shi-Yu Li, Jun Gu, Li-Hua Huang
Summary: A rapid identification method based on PCR-RFLP was developed to distinguish the two strains of FAW and other lepidopteran pests. The unique digestion patterns of four species, including FAW, were revealed by sequencing their mitochondrial COI genes. Furthermore, a specific SNP site was found in the corn strain FAW, allowing its distinction from the rice strain. Testing 28 FAW samples collected from different locations and host plants in China confirmed the presence of the corn strain only, indicating that the rice strain has not invaded China yet. This method enables differentiation of FAW from other lepidopteran pests and identification of the two FAW host strains.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Infectious Diseases
Purna Dwivedi, Mukul Sharma, Pushpendra Singh
Summary: This study presents a novel method for genotyping Mycobacterium leprae, which combines multiplex PCR amplification and RFLP analysis using AgeI restriction enzyme. This method allows for the identification of the most prevalent 1D and 3I genotypes without the need for specialized equipment.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2023)
Article
Parasitology
Raquel Montanez-Gonzalez, Alexandra C. Vallera, Maria Calzetta, Verena Pichler, Rachel R. Love, Moussa W. Guelbeogo, Roch K. Dabire, Marco Pombi, Carlo Costantini, Frederic Simard, Alessandra della Torre, Nora J. Besansky
Summary: In this study, tandem PCR-RFLP assays were developed for accurate genotyping of inversion 2Rc in An. coluzzii. Using these assays, it was found that employing two PCR-RFLP assays in tandem could improve genotyping reliability, ensuring over 99% accuracy in genotypic assignments. This approach is simple, cost-effective, and widely accessible, providing a crucial tool for investigating eco-phenotypes relevant to malaria epidemiology and vector control.
PARASITES & VECTORS
(2021)
Article
Veterinary Sciences
Nestor G. Michel-Regalado, Miguel A. Ayala-Valdovinos, Jorge Galindo-Garcia, Theodor Duifhuis-Rivera, Abraham Virgen-Mendez
Summary: The study provided a preliminary estimate of the frequency of the pathogenic single nucleotide polymorphism (SNP) of PKD1 in Persian and Persian-related cat breeds in western Mexico, with 23.1% of cats found to be carriers. Genotyping assays can assist local breeders and veterinarians in the selection of breeding stocks to prevent the propagation of ADPKD.
JOURNAL OF FELINE MEDICINE AND SURGERY
(2022)
Article
Parasitology
Reza Saberi, Maryam Nakhaei, Mahdi Fakhar, Hossein Zarrinfar, Ali Sharifpour, Hajar Ziaei Hezarjaribi
Summary: This study found the presence of Acanthamoeba (T4 and T2 genotypes) in the bronchoalveolar lavage fluid (BALF) of immunocompetent patients with chronic respiratory disorders (CRD). Further investigation is needed to understand the role of Acanthamoeba spp. in the pathogenesis of lung infections.
PARASITOLOGY RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Walid S. Mousa, Eman E. Abdeen, Yamen M. Hegazy
Summary: Mycotic mastitis is a neglected and incurable chronic mastitis in sheep flock in many countries, with a prevalence of 16.7% in an Egyptian study area. The study identified risky practices among shepherds contributing to the spread of the disease, emphasizing the need for educational campaigns to increase awareness.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2021)
Article
Genetics & Heredity
Ching-Chi Chang, Benji Brayan Silva, Huai-Ying Huang, Ching-Yi Tsai, Ronilo Jose D. Flores, Lemmuel L. Tayo, Yu-Chang Tyan, Ming-An Tsai, Gail Everette M. Catulin, Kuo-Pin Chuang, Jenq-Lin Yang
Summary: The recent increase in popularity of pigeon racing can be attributed to the large prize money involved. A new genotyping method using KASP technology has been developed as a rapid, reliable, and cost-effective alternative to detect polymorphisms in pigeons.
Article
Infectious Diseases
Claudia Patricia Rico-Torres, Luis Fernando Valenzuela-Moreno, Hector Luna-Pasten, Carlos Cedillo-Pelaez, Dolores Correa, Elizabeth Morales-Salinas, Jose Juan Martinez-Maya, Bruna Farias Alves, Hilda Fatima Jesus Pena, Heriberto Caballero-Ortega
Summary: Genotyping and virulence studies of Toxoplasma gondii strains from different animal species in Mexico revealed six different genotypes, seven microsatellite genotypes, and three different allele combinations of virulence markers. The study also highlighted the high genetic diversity of T. gondii in Mexico and predicted high virulence in some isolates in mice.
INFECTION GENETICS AND EVOLUTION
(2023)
Article
Microbiology
Daniella van der Walt, Emma T. Steenkamp, Brenda D. Wingfield, P. Markus Wilken
Summary: Hybridization is an important driver of evolution and adaptation, and closely related species of Ceratocystis have been shown to hybridize. However, previous studies using self-sterile strains and a laboratory-generated sterile isolate type may have impacted conclusions. This study successfully produced hybrids between self-fertile isolates of Ceratocystis species and provided evidence of biparental mitochondrial inheritance. Further research is needed to investigate the role of hybridization in the speciation of Ceratocystis species and the effect of mitochondrial conflict.
Review
Parasitology
J. P. Dubey, F. H. A. Murata, C. K. Cerqueira-Cezar, O. C. H. Kwok, C. Su
Summary: Toxoplasma gondii infections are common in humans and animals worldwide, with wild and domestic avian species playing a crucial role in the dissemination of the infection. Humans can become infected by consuming undercooked avian tissues. The genetic diversity of T. gondii strains isolated from various avian species worldwide from 2009 to 2020 is discussed, as well as the role of migratory birds in spreading T. gondii infection.
Review
Parasitology
J. P. Dubey, F. H. A. Murata, C. K. Cerqueira-Cezar, O. C. H. Kwok, C. Su
Summary: This review provides a comprehensive overview of Toxoplasma gondii infections in wild rodents worldwide, including prevalence, persistence of infection, clinical disease, epidemiology, and genetic diversity. Data are presented in tables organized by country, rodent species, and chronologically, making it easier for researchers to access information. The recent genetic diversity of T. gondii strains in rodents is critically evaluated in the review.
JOURNAL OF PARASITOLOGY
(2021)
Article
Infectious Diseases
Shilin Xin, Nan Jiang, Liulu Yang, Niuping Zhu, Wei Huang, Junbao Li, Longxian Zhang, Chunlei Su, Yurong Yang
Summary: This study identified a case of Toxoplasma gondii infection in a rhesus monkey collected from zoos in Henan Province, China. A new strain of T. gondii, designated as TgMonkeyCHn1, was successfully isolated from the monkey's myocardium, with unique characteristics in genotype and virulence genes. This is the first T. gondii strain isolated from Old World monkeys, demonstrating a new host record for T. gondii.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Veterinary Sciences
Jitender P. Dubey, Fernando H. A. Murata, Camila K. Cerqueira-Cezar, Oliver C. H. Kwok, Yurong Yang, Chunlei Su
Summary: Toxoplasma gondii infections are common in humans and animals worldwide, with New World NHP being highly susceptible to infection while Old World NHP show no reports of clinical toxoplasmosis. Susceptibility to clinical toxoplasmosis varies among different genera of New World NHP, with factors affecting this susceptibility not fully understood. Genetic characteristics of T. gondii strains from monkeys are summarized.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Ecology
Aline Diniz Cabral, Chunlei Su, Rodrigo Martins Soares, Solange Maria Gennari, Marcia Aparecida Speranca, Adriana Ruckert da Rosa, Hilda Fatima Jesus Pena
Summary: Studies in Sao Paulo state, Brazil, investigated the molecular frequency and diversity of Sarcocystidae parasites in bats, revealing a variety of these organisms in different bat species. The findings suggest that bats may play a role in the transmission of these parasites and highlight the need to consider Toxoplasma gondii as a differential diagnosis for rabies and other neurological syndromes in bats.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE
(2021)
Review
Parasitology
J. P. Dubey, F. H. A. Murata, C. K. Cerqueira-Cezar, O. C. H. Kwok, C. Su
Summary: Toxoplasma gondii infections are common in humans and animals worldwide, including in bears. Black bears have one of the highest seroprevalence rates of T. gondii. Clinical toxoplasmosis is rare in bears, with most cases reported in young bears. Genetic diversity of T. gondii isolates in bears is relatively high, ranging from strains associated with domestic cycles to those mainly found in wildlife.
JOURNAL OF PARASITOLOGY
(2021)
Review
Parasitology
Jitender P. Dubey, Fernando H. A. Murata, Camila K. Cerqueira-Cezar, Oliver C. H. Kwok, Chunlei Su, Michael E. Grigg
Summary: Toxoplasma gondii infections in kangaroos and other marsupials in Australia and New Zealand are common and may pose a risk for human infection. Kangaroo meat could potentially serve as a source of T. gondii infection for humans. The genetic diversity of T. gondii strains varies among different regions and species.
PARASITES & VECTORS
(2021)
Article
Ecology
Sawsan Ammar, Liberty Wood, Chunlei Su, Maria Spriggs, Justin Brown, Kyle Van Why, Richard Gerhold
Summary: Raptors from different regions in the United States showed varying seroprevalence rates of T. gondii. There were no significant differences in T. gondii exposure between raptors from different regions, sexes, or age groups. One red-shouldered hawk was found to harbor viable T. gondii tachyzoites, highlighting the need for further research to better understand the prevalence and impact of T. gondii in raptors in the US.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE
(2021)
Review
Veterinary Sciences
Yurong Yang, Shilin Xin, Fernando H. A. Murata, Camila K. Cerqueira-Cezar, Oliver C. H. Kwok, Chunlei Su, Jitender P. Dubey
Summary: The study provides an overview of Toxoplasma gondii infection in bats, including seroprevalence, genotypes, clinical cases, and transmission routes. Insectivorous bats have higher seroprevalence of T. gondii compared to fructivores bats; bats may play a significant role in the transmission and epidemiology of T. gondii. Additional research is needed to validate serological and molecular tests, and explore the transmission routes of T. gondii infection in bats.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Ecology
Katherine Kurth, Tiantian Jiang, Lisa Muller, Chunlei Su, Richard W. Gerhold
Summary: The study aimed to detect Toxoplasma gondii contamination by surveilling soil, wildlife, cats, and cows on a farm in Tennessee, U.S. Results showed that different contamination indicators can greatly affect the detection of T. gondii.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE
(2021)
Article
Virology
Trevor J. Hancock, Peyton Hickman, Niloo Kazerooni, Melissa Kennedy, Stephen A. Kania, Michelle Dennis, Nicole Szafranski, Richard Gerhold, Chunlei Su, Tom Masi, Stephen Smith, Tim E. Sparer
Summary: We report cross-reactive antibodies specific for SARS-CoV RBD from prepandemic cats and postpandemic South Carolina white-tailed deer. There are several potential explanations for this cross-reactivity, each with important implications to coronavirus disease surveillance.
JOURNAL OF VIROLOGY
(2022)
Article
Parasitology
Emilie Bouchard, Rajnish Sharma, Adrian Hernandez-Ortiz, Kayla Buhler, Batol Al-Adhami, Chunlei Su, Heather Fenton, Geraldine G.-Gouin, James D. Roth, Chloe Warret Rodrigues, Carla Pamak, Audrey Simon, Nicholas Bachand, Patrick Leighton, Emily Jenkins
Summary: This study investigated the seroprevalence and tissue infection rate of Toxoplasma gondii in foxes in northern Canada. The findings showed a higher antibody detection rate in older foxes. There was a correlation between the seroprevalence of foxes and humans in different regions of Canada.
PARASITES & VECTORS
(2022)
Review
Immunology
Rafael Calero-Bernal, Mercedes Fernandez-Escobar, Frank Katzer, Chunlei Su, Luis Miguel Ortega-Mora
Summary: Toxoplasma gondii shows significant genetic and phenotypic diversity. Different haplogroups of T. gondii exhibit different phenotypic characteristics in laboratory mouse infections. While mortality rate is an important phenotypic marker, additional non-lethal parameters could provide promising discriminatory power for field isolates. There is a need for consistent methods in phenotypic characterization and definition of virulence.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Alfred Chikweto, Andy Alhassan, Chunlei Su, Calum Macpherson, Muhammad Iqbal Bhaiyat, Jitender P. Dubey
Summary: This study investigated the genotypes and diversity of T. gondii in pigs in Grenada, West Indies, revealing a lower genetic diversity compared to other Caribbean areas, with ToxoDB #2 being the most common genotype.
VETERINARY MEDICINE AND SCIENCE
(2022)
Article
Veterinary Sciences
Sawsan Ammar, Nathan Hoggard, Liberty Wood, Chunlei Su, Richard Gerhold
Summary: Toxoplasmosis, caused by the protozoan parasite Toxoplasma gondii, can infect turkeys and may lead to signs of infection at high doses. The antibody titers, weight gain, and feed conversion rate in infected birds are influenced by the dose and strain of T. gondii. A significant decrease in weight gain was observed at 14 days postinfection in birds infected with a high dose of virulent T. gondii tachyzoites. Detection of T. gondii DNA was most common in the brain and heart, followed by the bursa of Fabricius and lungs. Continued experimental infection studies in wild and domestic animals are recommended given the growing trend of outdoor-raised livestock and wildlife consumption.
