Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 229, Issue -, Pages 8-11Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2015.12.005
Keywords
Quartz crystal microbalance; Probe-free biosensor; HPV16 DNA measurement; Loop-mediated isothermal amplification; TaqMan-qPCR; Cervical cancer
Funding
- Office of the Higher Education Commission, Ministry of Education, Thailand
- National Research Council of Thailand
Ask authors/readers for more resources
We have previously developed quartz crystal microbalance biosensor integrated with loop-mediated isothermal amplification (LAMP-QCM) for human papillomavirus (HPV) type58 DNA detection. Infection with HPV, particularly HPV16, remains a serious health problem due to its major risk factor contributing to cervical cancer. In the present study, LAMP-QCM biosensor was evaluated in terms of a quantitative assay for copy number of HPV16 DNA in cervical samples compared to quantitative PCR using TaqMan assay (TaqMan-qPCR). The detection limit of LAMP-QCM was found to be 10 fold more sensitive than TaqMan-qPCR with 100% specificity and 7.6% imprecision. Different plot of HPV16 DNA copy number using Bland-Altman analysis revealed 94% correlation between LAMP-QCM and qPCR. We therefore concluded that the developed LAMP-QCM biosensor provides a possible rapid and sensitive assay for HPV16 DNA quantification in a routine laboratory. (C) 2015 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available