Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 233, Issue -, Pages 15-22Publisher
ELSEVIER
DOI: 10.1016/j.jviromet.2016.03.007
Keywords
Surface plasmon resonance; Optical fiber; Rapid detection; Avian influenza virus subtype H6
Funding
- National Natural Science Foundation of China [31501582]
- Key Laboratory for Green Chemical Process of Ministry of Education [GCP201506]
- Hubei Key Laboratory of Novel Reactor and Green Chemical Technology [NRGCT201506]
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A side-polished fiber optic surface plasmon resonance (SPR) sensor was fabricated to expose the core surface and then deposited with a 40 nm thin gold film for the near surface sensing of effective refractive index changes with surface concentration or thickness of captured avian influenza virus subtype H6. The detection surface of the SPR optical fiber sensor was prepared through the plasma modification method for binding a self-assembled monolayer of isopropanol chemically on the gold surface of the optical fiber. Subsequently, N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide/N-hydroxysuccinimide was activated to enable EB2-B3 monoclonal antibodies to capture A/chicken/Taiwan/2838V/00 (H6N1) through a flow injection system. The detection limit of the fabricated optical fiber sensor for A/chicken/Taiwan/2838V/00 was 5.14 x 10(5) EID50/0.1 mL, and the response time was 10 min on average. Moreover, the fiber optic sensor has the advantages of a compact size and low cost, thus rendering it suitable for online and remote sensing. The results indicated that the optical fiber sensor can be used for epidemiological surveillance and diagnosing of avian influenza subtype H6 rapidly. (C) 2016 Elsevier B.V. All rights reserved.
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