Validation of an IFNγ/IL2 FluoroSpot assay for clinical trial monitoring

Antibody and T-cell responses associated with experimental human malaria infection or vaccination show limited relationships

(2015) Karen M. Walker et al.   IMMUNOLOGY

Guidelines for the automated evaluation of Elispot assays

(2015) Sylvia Janetzki et al.   Nature Protocols

Comparison of intramuscular and subcutaneous administration of a herpes zoster live-attenuated vaccine in adults aged ≥50 years: A randomised non-inferiority clinical trial

(2015) Javier Diez-Domingo et al.   VACCINE

The External Quality Assurance Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunospot (IFN-γ ELISpot) assay

(2014) Ana M. Sanchez et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Stepping up ELISpot: Multi-Level Analysis in FluoroSpot Assays

(2014) Sylvia Janetzki et al.   Cells

Standardization and Cross Validation of Alloreactive IFNγ ELISPOT Assays Within the Clinical Trials in Organ Transplantation Consortium

(2013) I. Ashoor et al.   AMERICAN JOURNAL OF TRANSPLANTATION

Cross-Validation of IFN-γ Elispot Assay for Measuring Alloreactive Memory/Effector T Cell Responses in Renal Transplant Recipients

(2013) O. Bestard et al.   AMERICAN JOURNAL OF TRANSPLANTATION

Validation of cell-based fluorescence assays: Practice guidelines from the ICSH and ICCS - part V - assay performance criteria

(2013) Brent Wood et al.   CYTOMETRY PART B-CLINICAL CYTOMETRY

The simultaneous ex vivo detection of low-frequency antigen-specific CD4+ and CD8+ T-cell responses using overlapping peptide pools

(2012) Satwinder Kaur Singh et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

T Cell Assays and MIATA: The Essential Minimum for Maximum Impact

(2012) C.M. Britten et al.   IMMUNITY

Comparison of Plasmid Vaccine Immunization Schedules Using IntradermalIn VivoElectroporation

(2011) David Hallengärd et al.   Clinical and Vaccine Immunology

Recommendations from the iSBTc-SITC/FDA/NCI Workshop on Immunotherapy Biomarkers

(2011) L. H. Butterfield et al.   CLINICAL CANCER RESEARCH

ELISpot for measuring human immune responses to vaccines

(2011) Meredith Slota et al.   Expert Review of Vaccines

Response definition criteria for ELISPOT assays revisited

(2010) Z. Moodie et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Minimal information about T cell assays: the process of reaching the community of T cell immunologists in cancer and beyond

(2010) C. M. Britten et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Multifunctional CD4+ T cells correlate with active Mycobacterium tuberculosis infection

(2010) Nadia Caccamo et al.   EUROPEAN JOURNAL OF IMMUNOLOGY

Recommendations for the validation of flow cytometric testing during drug development: II assays

(2010) Denise M. O'Hara et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Improved Endpoints for Cancer Immunotherapy Trials

(2010) A. Hoos et al.   JNCI-Journal of the National Cancer Institute

“MIATA”—Minimal Information about T Cell Assays

(2009) Sylvia Janetzki et al.   IMMUNITY

Precision and linearity targets for validation of an IFNγ ELISPOT, cytokine flow cytometry, and tetramer assay using CMV peptides

(2008) Holden T Maecker et al.   BMC IMMUNOLOGY

Concordant Proficiency in Measurement of T-Cell Immunity in Human Immunodeficiency Virus Vaccine Clinical Trials by Peripheral Blood Mononuclear Cell and Enzyme-Linked Immunospot Assays in Laboratories from Three Continents

(2008) M. J. Boaz et al.   Clinical and Vaccine Immunology

Polyfunctional T cell responses are a hallmark of HIV-2 infection

(2008) Melody G. Duvall et al.   EUROPEAN JOURNAL OF IMMUNOLOGY

The intracellular detection of MIP-1beta enhances the capacity to detect IFN-gamma mediated HIV-1-specific CD8 T-cell responses in a flow cytometric setting providing a sensitive alternative to the ELISPOT

(2008) Sarah Kutscher et al.   AIDS Research and Therapy