Stable flies sense and behaviorally respond to the polarization of light

Insects use their polarization-sensitive photoreceptors in a variety of ecological contexts including host-foraging. Here, we investigated the effect of polarized light on host foraging by the blood-feeding stable fly, Stomoxys calcitrans, a pest of livestock. Electroretinogram recordings with chromatic adaptation demonstrated that the spectral sensitivity of stable flies resembles that of other calyptrate flies. Histological studies of the flies' compound eye revealed differences in microvillar arrangement of ommatidial types, assumed to be pale and yellow, with the yellow R7 and pale R8 photoreceptors having the greatest polarization sensitivity. In behavioural experiments, stable flies preferred to alight on horizontally polarized stimuli with a high degree of linear polarization. This preferential response disappeared when either ultraviolet (UV) or human-visible wavelengths were omitted from light stimuli. Removing specific wavelength bands further revealed that the combination of UV (330-400 nm) and blue (400-525 nm) wavelength bands was sufficient to enable polarized light discrimination by flies. These findings enhance our understanding of polarization vision and foraging behavior among hematophagous insects and should inform future trap designs.

Automated summary

This study investigated the effect of polarized light on host foraging by stable flies. The results showed that stable flies prefer to alight on horizontally polarized stimuli with a high degree of linear polarization, and this preference disappeared when ultraviolet (UV) or human-visible wavelengths were omitted. Further experiments revealed that the combination of UV and blue wavelength bands was sufficient for the flies to discriminate polarized light. These findings contribute to our understanding of polarization vision and foraging behavior among blood-feeding insects and have implications for trap design.