Review
Food Science & Technology
Carine da Fonseca Cechin, Gabriela Guimaraes Carvalho, Caroline Peixoto Bastos, Dirce Yorika Kabuki
Summary: This review article discusses the occurrence and prevalence of Cronobacter spp. in plant-based foods, as well as the possible sources and routes of contamination, and the presence of pathogenic strains. The study found that Cronobacter can be found in a variety of plant-based foods, cultivation environments, and food processing industries. It also identified sequence types associated with clinical cases and antibiotic-resistant isolates.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Environmental Sciences
Raquel Castillo-Contreras, Marta Marin, Jorge Ramon Lopez-Olvera, Teresa Ayats, Xavier Fernandez Aguilar, Santiago Lavin, Gregorio Mentaberre, Marta Cerda-Cuellar
Summary: Campylobacter and Salmonella are the most commonly reported zoonotic agents in Europe. Wild boars in Barcelona have been found to carry both these pathogens. The prevalence of Campylobacter in wild boars is 61%, with some isolates showing antimicrobial resistance and virulence potential. Salmonella, including a serovar associated with human infections, was also detected in a small percentage of wild boars.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Food Science & Technology
Qi Li, Chengsi Li, Qinghua Ye, Qihui Gu, Shi Wu, Youxiong Zhang, Xianhu Wei, Liang Xue, Moutong Chen, Haiyan Zeng, Jumei Zhang, Qingping Wu
Summary: This study investigated the prevalence of Cronobacter spp. in wet rice and flour products from Guangdong province, China. The isolates were characterized in terms of molecular characteristics and antimicrobial susceptibility profiles. Out of 249 samples, 100 (40.16%) were positive for Cronobacter spp., with C. sakazakii O2 being the most dominant serotype. The study also identified new multilocus sequence typing patterns. The high contamination level and presence of pathogenic STs in the samples suggested a potential risk to public health.
CURRENT RESEARCH IN FOOD SCIENCE
(2023)
Article
Biology
Olga M. Zajac, Stefan Tyski, Agnieszka E. Laudy
Summary: Nosocomial infections caused by Stenotrophomonas maltophilia have been increasing worldwide. These bacteria are intrinsically resistant to most antibiotics. The aim of this study was to analyze the genetic diversity of isolates derived from various clinical materials, including blood, and the prevalence of multidrug-resistant efflux pump genes and susceptibility profiles in S. maltophilia. The research was conducted on 94 S. maltophilia isolates derived from hospitalized patients and outpatients in Warsaw, Poland. All isolates were susceptible to trimethoprim-sulfamethoxazole and minocycline, while 44/94 isolates demonstrated reduction in susceptibility to levofloxacin. A clonal relationship was observed among two groups of bloodstream isolates, and the presence of genes encoding ten different efflux pumps was shown in the majority of the isolates. The obtained knowledge about the prevalence of efflux pump genes in clinical S. maltophilia strains makes it possible to predict the scale of the risk of resistance emergence.
Article
Food Science & Technology
Rajae Zahli, Ann Karolin Scheu, Jamal Abrini, Jose L. Copa-Patino, Amajoud Nadia, Skali Senhaji Nadia, Juan Soliveri
Summary: The prevalence of Salmonella in poultry samples was found to be 9.84%, with major serovars including S. Hadar, S. Typhimurium, and S. Chester. 80.95% of Salmonella isolates were resistant to at least one antibiotic. The study also detected nine different MLST sequence types, with ST1954 being the most common.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Microbiology
Peng Fei, He Jing, Yan Ma, Gege Dong, Yunhe Chang, Zhaoxu Meng, Shilong Jiang, Qinggang Xie, Shuzhen Li, Xi Chen, Weiwei Yang
Summary: The study investigated the prevalence and characteristics of Cronobacter spp. in commercial powdered infant formula (PIF) from nine provinces in China. It found that Cronobacter spp. contamination was common in PIF, with the main sequence types being ST4, ST1, and ST64, and the dominant serotype being C. sakazakii serotype O2. Most isolates were susceptible to commonly used antibiotics, but showed resistance to cephalothin and vancomycin.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Luiza Vasconcellos, Samara Verly Silva, Luciana Veloso da Costa, Rebeca Vitoria da Silva Lage de Miranda, Cristhiane Moura Falavina dos Reis, Lygia Maria Paulo da Silva Braga, Claudiane Silva, Greice Conceicao, Josiane Mattoso, Igor Barbosa Silva, Stephen J. Forsythe, Victor Midlej, Maria Helena Simoes Villas Boas, Marcelo Luiz Lima Brandao
Summary: This study characterized 38 strains of Acinetobacter baumannii complex isolated from a biopharmaceutical industry. The results showed that some strains exhibited resistance to certain antibiotics and disinfectants, and formed persistent biofilms.
LETTERS IN APPLIED MICROBIOLOGY
(2023)
Article
Food Science & Technology
Xue Qin, Yitian Wang, Hongxuan Li, Xinyan Yang, Zhenghui Wang, Qing Chen, Chaoxin Man, Yujun Jiang
Summary: A visual detection strategy based on DNAzyme and asymmetry recombinase polymerase amplification (aRPA) was developed for monitoring Cronobacter sakazakii in powdered infant formula (PIF). The method showed low limits of detection and good stability in on-site simulation evaluation.
Article
Food Science & Technology
Monika Garbowska, Anna Berthold-Pluta, Lidia Stasiak-Rozanska, Antoni Pluta, Stephen Forsythe, Ilona Stefanska
Summary: This study investigated the genotyping diversity and hemolytic properties of Cronobacter spp. strains isolated from commercial ready-to-eat plant-based foods. The results showed high genotypic diversity and pathogenicity of these strains. Several new sequence types and alleles were also discovered.
Article
Biotechnology & Applied Microbiology
Segolene Calvez, Nora Navarro-Gonzalez, Charlene Siekoula-Nguedia, Catherine Fournel, Eric Duchaud
Summary: This study assessed the genetic diversity of Flavobacterium psychrophilum isolates from apparently healthy rainbow trout from several fish farms in the same watershed in France. High diversity was revealed by two different genotyping methods, with the majority of isolates being unrelated to the predominant clonal complex worldwide. Thirteen novel sequence types were discovered, suggesting a diverse subpopulation of F. psychrophilum in rainbow trout.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Seza Arslan, Hafize Gizem Erturk
Summary: The study found Cronobacter spp. contamination in popular ready-to-eat food products, with the highest contamination rates in spices, meat-free cig koftes, and desserts. The isolates showed resistance to multiple antibiotics, posing a potential risk to human health.
CURRENT MICROBIOLOGY
(2021)
Article
Microbiology
Mariana Meneguzzi, Caroline Pissetti, Raquel Rebelatto, Julian Trachsel, Suzana Satomi Kuchiishi, Adrienny Trindade Reis, Roberto Mauricio Carvalho Guedes, Joice Aparecida Leao, Caroline Reichen, Jalusa Deon Kich
Summary: Clinical salmonellosis has been increasing significantly in Brazil, with a majority of strains demonstrating high rates of multi-drug resistance. The identification of a major Choleraesuis clonal group in several Brazilian states suggests closely related strains. This highlights the endemic nature of salmonellosis in key pig-producing regions in Brazil.
Article
Food Science & Technology
Hongxuan Li, Shiqian Fu, Danliangmin Song, Xue Qin, Wei Zhang, Chaoxin Man, Xinyan Yang, Yujun Jiang
Summary: In this study, 35 Cronobacter strains isolated from powdered infant formula and its processing environment were identified and typed using 16S rRNA sequencing and multilocus sequence typing (MLST) technology. A total of 35 different sequence types were obtained, including three new ones that were isolated for the first time. The antibiotic resistance analysis revealed that all isolates were resistant to erythromycin but sensitive to ciprofloxacin. Moreover, 68.57% of the strains showed multi-drug resistance, with the most drug-resistant Cronobacter strain exhibiting resistance to 13 different drugs. Transcriptomics analysis identified 77 differentially expressed genes related to drug resistance, and the study further revealed that Cronobacter strains can activate multidrug efflux systems to enhance drug resistance. Understanding the drug resistance and mechanisms of Cronobacter is of great public health significance for the rational selection and development of antibacterial drugs, as well as the control and treatment of infections caused by this pathogenic microorganism.
Article
Food Science & Technology
Hua Jiang, Yue Xiang, Xiaojuan He, Chencheng Li, Fuxing Lin, Jihong Shao, Yuanhong Li
Summary: This study investigated the prevalence and characterization of Cronobacter spp. in dried edible mushrooms in Jiangsu province, China. The overall prevalence of Cronobacter spp. in dried edible mushrooms was 14.8%, with high genetic diversity observed. Some Cronobacter strains showed resistance to certain antibiotics, although they were susceptible to most tested antibiotics. Most isolates showed weak biofilm formation ability. This study provides new epidemiological evidence for the presence of Cronobacter spp. in dried edible mushrooms.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Food Science & Technology
Frank Lake, Leo S. van Overbeek, Johan J. P. Baars, Jeroen Koomen, Tjakko Abee, Heidy M. W. den Besten
Summary: Listeria monocytogenes, a foodborne pathogen, was isolated from food and food processing environments. This study characterized L. monocytogenes strains from frozen sliced mushroom production and processing environments. The strains showed diversity in PCR serogroups and were genotyped by whole genome sequencing. The presence of pathogenic islands and antibiotic susceptibility were observed, emphasizing the importance of hygienic measures in controlling L. monocytogenes in the production chain.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)