Article
Oncology
Sara Monteiro-Reis, Joao P. S. Ferreira, Ricardo A. Pires, Joao Lobo, Joao A. Carvalho, Rui L. Reis, Renato Natal Jorge, Carmen Jeronimo
Summary: This study found that the presence of a tumor can alter the stiffness of the bladder wall, which may affect the progression of bladder cancer. Using atomic force microscopy, the researchers measured the micromechanical properties of the bladder wall layers and observed that tumors promote the development of muscle-like structures in the mucosa.
Review
Biochemistry & Molecular Biology
Brisa Pena, Mostafa Adbel-Hafiz, Maria Cavasin, Luisa Mestroni, Orfeo Sbaizero
Summary: Arrhythmogenic cardiomyopathy is an inherited heart muscle disorder characterized by progressive replacement of cardiomyocytes, ventricular dilatation, cardiac dysfunction, arrhythmias, and sudden cardiac death. Molecular biomechanics for these disorders is becoming increasingly important. Atomic force microscopy (AFM) is a well-established technique for studying the mechanobiology of biological samples at the cellular scale. This review discusses the potential of AFM as a tool for assessing the biomechanics involved in arrhythmogenic cardiomyopathy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Xinyu Li, Li Zhao, Rongrong Feng, Xiaowei Du, Zelin Guo, Yu Meng, Yulan Zou, Wenchao Liao, Qiyuan Liu, Yaohuan Sheng, Gaowei Zhao, Haijian Zhong, Weidong Zhao
Summary: Using atomic force microscopy, it was found that Na(V)1.5 is irregularly distributed on the surfaces of both normal and cancer breast cells, with higher expression on cancer cells. The stability of the Na(V)1.5-antibody complex is higher on normal breast cells compared to cancer breast cells. These findings provide insights into the interactions of ion channel-antibody systems and the role of sodium channels in tumor metastasis and invasion.
ANALYTICAL METHODS
(2023)
Article
Multidisciplinary Sciences
Connor Stashko, Mary-Kate Hayward, Jason J. Northey, Neil Pearson, Alastair J. Ironside, Johnathon N. Lakins, Roger Oria, Marie-Anne Goyette, Lakyn Mayo, Hege G. Russnes, E. Shelley Hwang, Matthew L. Kutys, Kornelia Polyak, Valerie M. Weaver
Summary: Intratumor heterogeneity is associated with poor patient outcome, and stromal stiffening accompanies cancer. It is unclear whether cancers exhibit stiffness heterogeneity and its link to tumor cell heterogeneity. A new method called Spatially Transformed Inferential Force Map (STIFMap) has been developed to measure stiffness heterogeneity in human breast tumors, allowing the quantification of stromal stiffness experienced by each cell and visual registration with tumor progression biomarkers.
NATURE COMMUNICATIONS
(2023)
Article
Nanoscience & Nanotechnology
Binyu Zhao, Youquan Jia, Yi Xu, Elmar Bonaccurso, Xu Deng, Guenter K. Auernhammer, Longquan Chen
Summary: Researchers have investigated the microscopic wetting phenomena of water on nonwetting nanoporous surfaces, characterizing the nanoscopic morphology and effective stiffness of liquid-air interfaces inside nanopores. They found that the nanomenisci exhibit apparent elastic deformation and size-dependent effective stiffness, and correlated these properties with the wetting behavior of water droplets on structured surfaces.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Nanoscience & Nanotechnology
Michael LeClaire, James A. Wohlschlegel, Helena Chang, Madhuri Wadehra, Weibo Yu, JianYu Rao, David Elashoff, James K. Gimzewski, Shivani Sharma
Summary: This study compared the structure-mechanical properties of breast cancer cell-derived sEVs and their secreting cells, revealing a decrease in Young's modulus of sEVs as cells progress from non-tumor to invasive breast cancer phenotypes. Additionally, analysis of sEVs from breast cancer patients showed alterations in biomechanical properties compared to healthy controls. The findings suggest that precise biomechanical fingerprinting of single nanoscale sEVs could be a useful approach for detecting changes in parental cells during malignant transformation.
ACS APPLIED NANO MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Barbara Zbiral, Andreas Weber, Maria dM. Vivanco, Jose L. Toca-Herrera
Summary: In healthy tissues, cells are mechanically stable. However, during cancer progression, cancer cells undergo changes in their mechanical properties, becoming softer and more fluid-like. This is associated with cytoskeletal remodeling, altered adhesion properties, increased cell proliferation, and enhanced cell motility. In our study, we examined the mechanical properties of different breast cancer cells using atomic force microscopy force spectroscopy. Our results revealed that the mechanical properties of the cells are linked to their malignancy, with tamoxifen-resistant MCF-7 cells being the softest and MCF10A cells being the stiffest. Confocal fluorescence microscopy further demonstrated differences in actin network organization and focal adhesion localization. These findings contribute to our understanding of the complex alterations involved in cancer development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Danahe Mohammed, Melanie Koehler, Andra C. Dumitru, Pavithra Aravamudhan, Danica M. Sutherland, Terence S. Dermody, David Alsteens
Summary: Breast cancer remains a major health issue for women, with aggressive invasive types posing a challenge to current therapies. Research shows that the overexpression of alpha-sialylated glycans in breast cancer cells presents an opportunity for combatting cancer cells with oncolytic reoviruses, offering new perspectives in oncolytic cancer therapy.
Article
Engineering, Multidisciplinary
A. Garduno-Medina, E. Munoz-Pineda, M. A. Vazquez-Delgado, V. Garcia-Vazquez, R. Espinosa-Luna, F. J. Flores-Ruiz
Summary: Resonant curves of AFM cantilevers were studied using experimental data and FEA modeling. An analytical function was used to determine effective stiffness and mass for each resonant mode. Hydration process led to a reduction in normal contact stiffness k(N).
Article
Microscopy
Li Zhao, Xiaowei Du, Bin Fang, Qiyuan Liu, Hui Yang, Fangzuo Li, Yaohuan Sheng, Xiangfu Zeng, Haijian Zhong, Weidong Zhao
Summary: Breast cancer, a common malignant cancer in women, remains poorly understood in terms of its pathogenesis. This study utilized Conductive Atomic Force Microscopy (CAFM) to investigate the electrical conductivity and electron transport of normal and cancer breast cells. It was found that cancer cells exhibited better electrical conductivity compared to normal cells, suggesting the potential for distinguishing cancer cells and advancing further research in this field.
Article
Materials Science, Multidisciplinary
Saeid Ekrami, Fabienne Quiles, Alice Schollhammer, Xavier Bellanger, Erwan Andre, Gregory Francius
Summary: In this study, the cross-linking reactions of hydrogels based on PAH and HA were examined. It was found that the choice of cross-linkers can significantly affect the mechanical and chemical properties of the hydrogels.
ACS APPLIED POLYMER MATERIALS
(2023)
Article
Engineering, Biomedical
Auxtine Micalet, Judith Pape, Deniz Bakkalci, Yousef Javanmardi, Chloe Hall, Umber Cheema, Emad Moeendarbary
Summary: Tumors and host tissues are stiffer than conventionally used hydrogels for studying cancer progression in vitro. The tumoroid assay provides a biomimetic 3D in vitro tumor model to investigate cancer cell invasion in terms of extracellular matrix (ECM) composition and stiffness. The change in matrix stiffness caused by epithelial colorectal cancer cells is characterized by atomic force microscopy indentation tests, and is attributed to cell-generated forces and ECM degradation involving matrix metalloproteinases (MMPs).
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Seongoh Kim, Yunkyung Lee, Manhee Lee, Sangmin An, Sang-Joon Cho
Summary: Utilizing the newly developed PinPoint(TM) nanomechanical mode, AFM can quickly and accurately measure the Young's modulus of a sample's entire scan surface. This approach allows for simultaneous measurements of topographical and force-distance data at each pixel within the scan area, enabling quantitative visualization of pixel-by-pixel topographical height and Young's modulus of the entire scan surface.
Article
Nanoscience & Nanotechnology
Keivan Asadi, Junghoon Yeom, Hanna Cho
Summary: Investigating internal resonance (IR) mechanisms in micro/nanoresonators reveals that intermodal coupling between second and third flexural modes in asymmetric structures provides an optimal condition for strong IR, with high energy transfer to the resonated mode. This study introduces design strategies that can be easily integrated into typical micro/nanoelectromechanical systems, offering potential for paradigm-shifting applications in micro/nanosystems.
MICROSYSTEMS & NANOENGINEERING
(2021)
Article
Biophysics
Viktoriia Drebezghova, Florence Hakil, Regis Grimaud, Hubert Gojzewski, G. Julius Vancso, Corinne Nardin
Summary: The initial retention of Escherichia coli on PDMS surfaces was studied in relation to substrate bulk and surface mechanical stiffness values. The number of retained bacteria showed a decreasing trend with the increase of both bulk and surface mechanical stiffnesses, reaching a threshold corresponding to the Young's modulus of the bacterial cell surface, beyond which the number of retained bacteria remained constant.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Cardiac & Cardiovascular Systems
Alireza Rezvani-Sharif, Mohammad Tafazzoli-Shadpour, Alberto Avolio
CARDIOVASCULAR ENGINEERING AND TECHNOLOGY
(2019)
Article
Biophysics
Azim Parandakh, Azadeh Anbarlou, Mohammad Tafazzoli-Shadpour, Abdolreza Ardeshirylajimi, Mohammad-Mehdi Khani
COLLOIDS AND SURFACES B-BIOINTERFACES
(2019)
Article
Engineering, Biomedical
Atefeh Jannatbabaei, Mohammad Tafazzoli-Shadpour, Ehsan Seyedjafari, Nasser Fatouraee
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2019)
Article
Mechanics
Farzaneh Safshekan, Mohammad Tafazzoli-Shadpour, Majid Abdouss, Mohammad B. Shadmehr, Fariba Ghorbani
INTERNATIONAL JOURNAL OF SOLIDS AND STRUCTURES
(2020)
Article
Multidisciplinary Sciences
Atefeh Jannatbabaei, Mohammad Tafazzoli-Shadpour, Ehsan Seyedjafari
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2020)
Article
Nanoscience & Nanotechnology
Arian Ansardamavandi, Mohammad Tafazzoli-Shadpour, Ramin Omidvar, Fatemeh Nili
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2020)
Article
Biophysics
Mahyar Ahmadpour-B, Ahmad Nooraeen, Mohammad Tafazzoli-Shadpour, Hadi Taghizadeh
Summary: Atherosclerosis initiation is related to abnormal hemodynamic parameters at arterial bifurcations, with severe stenosis causing significant localized changes in hemodynamic parameters. Mild stenosis has minor impact on hemodynamic parameters.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Amirhossein Emamian, Farzaneh Aghajani, Farzaneh Safshekan, Mohammad Tafazzoli-Shadpour
Summary: This study aimed to describe the viscoelastic behavior of human dentin and determine the best-fitting viscoelastic model. Despite a nearly linear trend at small strains, there was some degree of nonlinearity in dentine viscoelasticity. The modified superposition formulation was found to best capture the viscoelastic behavior of human dentin.
Article
Computer Science, Interdisciplinary Applications
Mohammad Tabatabaei, Mohammad Tafazzoli-Shadpour, Mohammad Mehdi Khani
Summary: The biophysical properties of cells change with cancer invasion, particularly affecting cell's viscoelastic behavior and mechanical properties of cytoskeleton fibers. Different types of cancer cells show differences in mechanical properties of actin fibers and microtubules, which is important for cancer progression.
MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING
(2021)
Article
Biochemistry & Molecular Biology
Ehsan Mohammadi, Mohammad Tabatabaei, Mahdi Habibi-Anbouhi, Mohammad Tafazzoli-Shadpour
Summary: This study investigates the effects of Aromatase Inhibitors and mTOR Inhibitors on the biological and physical behaviors of breast cancer cells, with a greater impact on invasive cells. By regulating the mechanical properties and biological behaviors of cells, these drugs emphasize the crosstalk between chemical and physical signaling pathways.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Arian Ansardamavandi, Mohammad Tafazzoli-Shadpour
Summary: The function of biological tissues is regulated at cellular level and is highly influenced by the physical microenvironment. In cancer, both chemical and physical signaling cascades play important roles in tumor growth and invasion, with cancer associated fibroblasts (CAFs) being crucial in this process. The interaction between cells and ECM, through mechanosensing pathways, defines the progression and invasion of cancer cells.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Mechanics
Milad Samaee, Ahmad Nooraeen, Mohammad Tafazzoli-Shadpour, Hadi Taghizadeh
Summary: Endothelial cells are crucial in maintaining arterial stability. Abnormal levels of hemodynamic parameters caused by pulsatile flow contribute to the formation and development of atherosclerotic plaques. This study examined the impact of Newtonian and non-Newtonian blood flows on the carotid bifurcation using an experimental setup. Results showed significant differences between the two flow regimes, especially near junction sites, with the non-Newtonian model exhibiting flattened velocity profiles and higher back flow during diastole.
Article
Pharmacology & Pharmacy
Maokai Xu, Maria Antonova, Pavel Salavei, Katharina Illek, Ana Valeria Melendez, Ramin Omidvar, Roland Thuenauer, Olga Makshakova, Winfried Roemer
Summary: In this study, a novel ligand-receptor pair based on dimeric LecA and host cell glycosphingolipid Gb3 was developed for drug delivery across cellular barriers. The trafficking kinetics and transcytosis efficiencies were characterized using immunofluorescence and confocal microscopy. Fusion proteins of LecA and EGFP were able to cross cellular monolayers, with EGFP-LecA showing a higher release rate compared to LecA-EGFP. Molecular dynamics simulations and cross-linking studies suggested that EGFP-LecA tends to be a dimer while LecA-EGFP forms a tetramer. These findings propose dimeric LecA chimeras as potential drug delivery tools through Gb3-positive cellular barriers.
Article
Chemistry, Multidisciplinary
Ramin Omidvar, Yareni A. Ayala, Annette Brandel, Lukas Hasenclever, Martin Helmstaedter, Alexander Rohrbach, Winfried Roemer, Josef Madl
Summary: The interaction between bacterial lectin LecA and host cell glycosphingolipid Gb3 is crucial for the cellular uptake of Pseudomonas aeruginosa, involving lipid zipper formation and full membrane engulfment of the bacterium. Nanoscale force characterization of this mechanism reveals that LecA-Gb3 interactions strengthen bacterial attachment to the membrane and reduce energy required for uptake.
Article
Biotechnology & Applied Microbiology
Shohreh Azadi, Mohammad Tafazzoli Shadpour, Majid E. Warkiani
Summary: The study investigated the impact of substrate stiffness on the extravasation ability of breast cancer cells. Softening of the substrate led to a reduction in invasion capability and migration distance, while stiffening increased these parameters. There was a positive correlation between MMP9 concentration and extravasation of cancer cells, suggesting it as a potential underlying mechanism mediated by substrate stiffness.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Engineering, Biomedical
Xinyao Zhu, Yifan Liu, Jing Ye, Wei Xu, Xuexia Zhao, Tianyan Liu
Summary: This study reveals the adverse effect of acid on dentin in terms of degradation of its fracture toughness. The peritubular dentin plays a significant role in enhancing the dentin's fracture resistance capability. The findings highlight the importance of structural integrity for dentin.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2024)
Article
Engineering, Biomedical
Priya Ranganathan, Vijayakumari Sugumaran, Bargavi Purushothaman, Ajay Rakkesh Rajendran, Balakumar Subramanian
Summary: The study aims to design and fabricate an ultra-easier multi-functional biomedical polymeric scaffold loaded with unique equimolar Ca:P phasic bioactive glass material. The results showed that the G:BG (1:2) ratio is the more appropriate composition for enhanced bio-mineralization and higher surface area. The scaffold can induce mitogenesis in osteoblast cells for hard tissue regeneration and rapid collagen secretion in fibroblast cells for soft tissue regeneration.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2024)
Article
Engineering, Biomedical
Ziad Guerfi, Oum keltoum Kribaa, Hanane Djouama
Summary: Hydroxyapatite, a biocompatible and bioactive ceramic material, has been widely studied in fields such as orthopedics and plastic surgery. The use of computational tools, especially density functional theory, has become increasingly important in research. In this study, Hydroxyapatite was synthesized using the double decomposition method and quantum mechanical computations were performed using density functional theory. The experimental and computational results confirmed the successful synthesis of Hydroxyapatite and showed good agreement in spectroscopic characterizations.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2024)
Article
Engineering, Biomedical
Sally AbdulHussain Kadhum, Nassier A. Nassir
Summary: In this research, porous composites were successfully prepared and reinforced for bone scaffold applications. The functional groups, pore structure, and composition distribution of the materials were characterized using techniques such as FTIR, Atomic Force Microscopy (AFM), and Scanning Electron Microscopy (SEM).
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2024)
Article
Engineering, Biomedical
Veronika Geiger, Felicitas Mayinger, Moritz Hoffmann, Marcel Reymus, Bogna Stawarczyk
Summary: The study investigated the mechanical properties of four additively manufactured denture base resins in different measurement environments, and found that the measurement environment impacts the strength and fracture toughness of the materials.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2024)
Article
Engineering, Biomedical
Junxiao Wang, Amatjan Sawut, Rena Simayi, Huijun Song, Xueying Jiao
Summary: The development of cost-effective and eco-friendly conductive hydrogels with excellent mechanical properties, self-healing capabilities, and non-toxicity is of great significance in the field of biosensors.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2024)
Article
Engineering, Biomedical
Yijun Zhou, Lisa Ho, Ayan Samanta, Philip Procter, Cecilia Persson
Summary: In this study, soft, non-setting biomaterials based on Hyalectin gels and different morphological parameters of hydroxyapatite (HA) particles were evaluated as potential augmentation materials for orthopaedic implant fixation. The results showed that constructs reinforced with irregularly shaped nano-HA particles and spherically shaped micro-HA particles had significantly higher pull-out force compared to the control group.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2024)
Article
Engineering, Biomedical
Mehdi Jahandardoost, Donald Ricci, Abbas S. Milani, Mohsen Jahandardoost, Dana Grecov
Summary: Tubular flow diverters are important for treating cerebral aneurysms. A new design called VR-eCLIPs has been developed to cover the neck of challenging bifurcation aneurysms. A finite element model has been used to simulate the implantation processes of VR-eCLIPs and assess potential plastic deformation.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2024)
Article
Engineering, Biomedical
Marek Traczynski, Adam Patalas, Katarzyna Roslan, Marcin Suszynski, Rafa l Talar
Summary: This article evaluates the forces acting on intravenous needles during insertion into the skin and selects the most suitable model for future research. The experimental results show that needle size, insertion angle, and insertion speed have an influence on the measured force values.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2024)
Article
Engineering, Biomedical
Chester Jar, Andrew Archibald, Monica Gibson, Lindsey Westover
Summary: This study evaluates the ASIST technique for assessing the stability of dental implants. The results show that the ASIST technique can reliably measure the interfacial stiffness of dental implants, which is not significantly influenced by different abutment types. This method may provide an improved non-invasive way to measure the stability of dental implants.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2024)
Article
Engineering, Biomedical
Ali Kamali, Kaveh Laksari
Summary: In this paper, a UNet-based neural network model (El-UNet) is developed to infer the spatial distributions of mechanical parameters. The El-UNet shows superior performance in terms of accuracy and computational cost compared to other neural network models. A self-adaptive spatial loss weighting approach is proposed, which achieves the most accurate reconstructions in equal computation times.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2024)
Article
Engineering, Biomedical
Chunyan Yu, Yanju Lu, Jinhui Pang, Lu Li
Summary: In this study, a safe and effective hemostatic composite sponge was developed by combining chitosan and hydroxypropylmethylcellulose (HPMC). The sponge exhibited excellent flexibility and rapid hemostatic ability in vitro. In vivo assessments showed that the sponge had the shortest clotting time and minimal blood loss.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2024)
Article
Engineering, Biomedical
Zhongliang Yu, Lin Yu, Junjie Liu
Summary: The study proposes incorporating functionally graded tablets into nacreous composites to enhance both stiffness and damping properties. Analytical formulae and numerical experiments demonstrate the effectiveness of this design, surpassing existing homogeneous composites in performance.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2024)
Article
Engineering, Biomedical
Marc Graham, Sandra Klinge
Summary: This study investigates the macroscopic diffusion behavior of heterogeneous gels using a homogenization method in a finite element framework. Two materials, calcifying PDMA and PAAm, were studied, and the results show that the diffusivity of PDMA has a strong nonlinear dependence on the solute molecule radius.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2024)
Article
Engineering, Biomedical
Abdur-Rasheed Alao
Summary: This study aimed to find the optimal sandblasting parameters for roughening YTZP surfaces. Through experimental and statistical analysis, the best setting was found to be IA = 45 degrees, AP = 110 μm, ST = 20 s, and P = 400 kPa, which resulted in the maximum surface roughness, phase transformation, and shear bond strength.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2024)
