4.6 Article

Fabrication of silver nanoparticle-doped hydroxyapatite coatings with oriented block arrays for enhancing bactericidal effect and osteoinductivity

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ELSEVIER
DOI: 10.1016/j.jmbbm.2016.04.002

Keywords

Hydroxyapatite coating; Silver nanoparticle; Antibacterial property; Osteogenic

Funding

  1. Key Disciplines of the Shanghai Municipal Education Commission [J50206]
  2. National Natural Science Foundation of China [51372152, 81572116, 81301590]
  3. Science and Technology Commission of Shanghai Municipality [12JC1405600]
  4. Program of Shanghai Normal University [DZL124, DCL201303]
  5. Innovation Foundation of Shanghai Education Committee [14ZZ124]

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Implant-associated infection is a common postoperative complication and remains a serious problem in orthopedic surgery. This work describes the synthesis of silver nanoparticle-doped hydroxyapatite coatings with oriented block arrays (AgNP-BHAC). The resulting nanostructure was investigated using scanning electron microscopy, energy-dispersive spectrometry, transmission electron microscopy, X-ray powder diffraction, and Fourier transform infrared spectroscopy. AgNP-BHAC exhibited excellent antimicrobial activity toward gram-negative Escherichia coli and gram-positive Staphylococcus aureus owing to the antibacterial effects of the silver nanoparticles. Human bone marrow stromal cells (hBMSC) culture revealed that the AgNP-BHAC exhibited better biocompatibility, and permitted improved cell proliferation, attachment, and osteoinductivity than uncoated Ti-6Al-4V titanium alloy, the favored material for biomedical applications. In summary, this study presents a convenient and effective method for the incorporation of silver into HA coatings with block morphology. This method can be utilized to modify a variety of metallic implant surfaces to improve their antimicrobial effects and reduce potential long-term cytotoxicity. (C) 2016 Elsevier Ltd. All rights reserved.

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