Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 138, Issue 14, Pages 4757-4762Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.5b12251
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Funding
- EPSRC [EP/K006630/1]
- Deutsche Forschungsgemeinschaft [SFB 1078]
- EPSRC
- EPSRC [EP/K006630/1, EP/M002144/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/K006630/1, EP/M002144/1] Funding Source: researchfish
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Channelrhodopsins are light-gated ion channels with extensive applications in optogenetics. Channelrhodopsin-1 from Chlamydomonas augustae (CaChR1) exhibits a red-shifted absorption spectrum as compared to Channelrhodopsin-2, which is highly beneficial for optogenetic application. The primary event in the photocycle of CaChR1 involves an isomerization of the protein-bound retinal chromophore. Here, we apply highly time-resolved vibronic spectroscopy to reveal the electronic and structural dynamics associated with the first step of the photocycle of CaChR1. We observe vibrationally coherent formation of the P-1 intermediate exhibiting a twisted 13-cis retinal with a 110+/- 7 fs time constant. Comparison with low-temperature resonance Raman spectroscopy of the corresponding trapped photoproduct demonstrates that this rapidly formed P-1 intermediate is stable for several hundreds of nanoseconds.
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