A review of the current state of single-cell proteomics and future perspective

Integrated single-cell (phospho-)protein and RNA detection uncovers phenotypic characteristics and active signal transduction of human antibody secreting cells

(2023) Erik van Buijtenen et al.   MOLECULAR & CELLULAR PROTEOMICS

Robust and Easy-to-Use One-Pot Workflow for Label-Free Single-Cell Proteomics

(2023) Manuel Matzinger et al.   Analytical Chemistry

Initial recommendations for performing, benchmarking and reporting single-cell proteomics experiments

(2023) Laurent Gatto et al.   NATURE METHODS

Label‐free single cell proteomics utilizing ultrafast LC and MS instrumentation: A valuable complementary technique to multiplexing

(2023) Manuel Matzinger et al.   PROTEOMICS

Uncovering biology by single-cell proteomics

(2023) M. Shahid Mansuri et al.   Communications Biology

In‐depth analysis of proteomic and genomic fluctuations during the time course of human embryonic stem cells directed differentiation into beta cells

(2022) Bogdan Budnik et al.   PROTEOMICS

Ultra‐high sensitivity mass spectrometry quantifies single‐cell proteome changes upon perturbation

(2022) Andreas‐David Brunner et al.   Molecular Systems Biology

Three-dimensional feature matching improves coverage for single-cell proteomics based on ion mobility filtering

(2022) Jongmin Woo et al.   Cell Systems

Single-Cell Chemical Proteomics (SCCP) Interrogates the Timing and Heterogeneity of Cancer Cell Commitment to Death

(2022) Ákos Végvári et al.   ANALYTICAL CHEMISTRY

Increasing the throughput of sensitive proteomics by plexDIA

(2022) Jason Derks et al.   NATURE BIOTECHNOLOGY

Insights into protein post-translational modification landscapes of individual human cells by trapped ion mobility time-of-flight mass spectrometry

(2022) Benjamin C. Orsburn et al.   Nature Communications

MicroPOTS Analysis of Barrett’s Esophageal Cell Line Models Identifies Proteomic Changes after Physiologic and Radiation Stress

(2021) Kenneth Weke et al.   JOURNAL OF PROTEOME RESEARCH

MaxDIA enables library-based and library-free data-independent acquisition proteomics

(2021) Pavel Sinitcyn et al.   NATURE BIOTECHNOLOGY

Towards resolving proteomes in single cells

(2021) Arunima Singh   NATURE METHODS

Quantitative single-cell proteomics as a tool to characterize cellular hierarchies

(2021) Erwin M. Schoof et al.   Nature Communications

Comparative Proteome Signatures of Trace Samples by Multiplexed Data-Independent Acquisition

(2021) Claudia Ctortecka et al.   MOLECULAR & CELLULAR PROTEOMICS

Scaling Up Single-Cell Proteomics

(2021) Nikolai Slavov   MOLECULAR & CELLULAR PROTEOMICS

Joint single-cell measurements of nuclear proteins and RNA in vivo

(2021) Hattie Chung et al.   NATURE METHODS

Multiplexed single-cell proteomics using SCoPE2

(2021) Aleksandra A. Petelski et al.   Nature Protocols

High-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip

(2021) Jongmin Woo et al.   Nature Communications

Unpicking the proteome in single cells

(2020) Nikolai Slavov   SCIENCE

Impact of the Identification Strategy on the Reproducibility of the DDA and DIA Results

(2020) Carolina Fernández-Costa et al.   JOURNAL OF PROTEOME RESEARCH

A Versatile Isobaric Tag Enables Proteome Quantification in Data-Dependent and Data-Independent Acquisition Modes

(2020) Xiaobo Tian et al.   ANALYTICAL CHEMISTRY

Boosting to Amplify Signal with Isobaric Labeling (BASIL) Strategy for Comprehensive Quantitative Phosphoproteomic Characterization of Small Populations of Cells

(2019) Lian Yi et al.   ANALYTICAL CHEMISTRY

TMT labeling for the masses: A robust and cost-efficient, in-solution labeling approach

(2019) Jana Zecha et al.   MOLECULAR & CELLULAR PROTEOMICS

DIA-NN: neural networks and interference correction enable deep proteome coverage in high throughput

(2019) Vadim Demichev et al.   NATURE METHODS

Nanodroplet processing platform for deep and quantitative proteome profiling of 10–100 mammalian cells

(2018) Ying Zhu et al.   Nature Communications

Single-Cell Mass Spectrometry for Discovery Proteomics: Quantifying Translational Cell Heterogeneity in the 16-Cell Frog (Xenopus ) Embryo

(2016) Camille Lombard-Banek et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

On the Dependency of Cellular Protein Levels on mRNA Abundance

(2016) Yansheng Liu et al.   CELL

Identification of Maturation-Specific Proteins by Single-Cell Proteomics of Human Oocytes

(2016) Irma Virant-Klun et al.   MOLECULAR & CELLULAR PROTEOMICS

A multicenter study benchmarks software tools for label-free proteome quantification

(2016) Pedro Navarro et al.   NATURE BIOTECHNOLOGY

Single-Cell Metabolomics: Changes in the Metabolome of Freshly Isolated and Cultured Neurons

(2012) Peter Nemes et al.   ACS Chemical Neuroscience

iTRAQ Labeling is Superior to mTRAQ for Quantitative Global Proteomics and Phosphoproteomics

(2011) Philipp Mertins et al.   MOLECULAR & CELLULAR PROTEOMICS

Evaluation of Metalloendopeptidase Lys-N Protease Performance under Different Sample Handling Conditions

(2010) Nadia Taouatas et al.   JOURNAL OF PROTEOME RESEARCH

Multiplex peptide stable isotope dimethyl labeling for quantitative proteomics

(2009) Paul J Boersema et al.   Nature Protocols