Review
Chemistry, Analytical
Shuang Ding, Haijun Zhang, Xuemei Wang
Summary: Lung diseases pose serious threats to human health worldwide, and traditional models are unable to accurately simulate lung conditions. Human organ-on-a-chip platforms and biosensors can better mimic lung characteristics, providing new methods for studying disease development and drug efficacy.
Review
Chemistry, Analytical
Yu-Jia Wei, Ya-Nan Zhao, Xuan Zhang, Xing Wei, Ming-Li Chen, Xu-Wei Chen
Summary: Nowadays, biochemical analysis, such as cell analysis, disease diagnosis and treatment, environmental monitoring, and food safety, is receiving increasing attention. Optically integrated microfluidic technology combines the benefits of high sensitivity, fast detection, and excellent selectivity. By utilizing flexible optical detection techniques and precisely-controlled fluidics, microfluidic chips enable high throughput analysis, reduced reagent consumption, and decreased assay expenditure. This review introduces the integration of microfluidic chips with various optical detection techniques and their applications in biochemical analysis, and also discusses the future prospects of optically integrated microfluidic chips.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Yan Zhang, Sungho Kim, Weihua Shi, Yaoyao Zhao, Insu Park, Christopher Brenden, Hrishikesh Iyer, Prasoon Jha, Rashid Bashir, Jonathan V. Sweedler, Yurii Vlasov
Summary: The silicon microfluidic platform allows for the monolithic integration of transparent micron-scale microfluidic channels, on-chip segmentation of analyte flows into picoliter droplets, and spatial and temporal separation of oil and aqueous phases during electro-spray for subsequent mass spectrometry analysis.
Review
Biochemical Research Methods
Long Ngo, Le Que Anh Pham, Anastasiia Tukova, Amin Hassanzadeh-Barforoushi, Wei Zhang, Yuling Wang
Summary: Cancer-derived small extracellular vesicles (sEVs) are specific subgroups of lipid bilayer vesicles secreted from cancer cells to the extracellular environment. They carry distinct biomolecules (e.g., proteins, lipids and nucleic acids) from their parent cancer cells. Therefore, the analysis of cancer-derived sEVs can provide valuable information for cancer diagnosis. However, the use of cancer-derived sEVs in clinics is still limited due to their small size, low amounts in circulating fluids, and heterogeneous molecular features, making their isolation and analysis challenging.
Article
Chemistry, Analytical
Yuting Shang, Gaowa Xing, Xuejiao Liu, Haifeng Lin, Jin-Ming Lin
Summary: A portable microfluidic biosensor has been developed for the detection of E. coli O157:H7 using finger actuation. The biosensor consists of three functional zones and is capable of separating and detecting the target bacteria through immunomagnetic separation, nucleic acid extraction and purification, and signal detection. It offers quantitative detection and high recovery rate, eliminating the need for external pumps and skilled personnel.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Zhenzhen Han, Xinyan Peng, Yi Yang, Jia Yi, Dan Zhao, Qiyuan Bao, Shuping Long, Sai-Xi Yu, Xin-Xin Xu, Baohong Liu, Yan-Jun Liu, Yuhui Shen, Liang Qiao
Summary: In this study, a method combining microfluidics and surface-enhanced Raman spectroscopy (SERS) was developed to isolate exosomes from plasma and profile exosomal biomarkers for osteosarcoma diagnosis. The method showed efficient isolation of exosomes directly from human plasma and could profile exosomes based on protein biomarkers with a low detection limit. The analysis of plasma-derived exosomes from osteosarcoma patients and healthy controls revealed significantly higher levels of specific protein biomarkers on exosomes from osteosarcoma patients. A classification model was built based on the exosomal biomarkers, achieving high sensitivity, specificity, and accuracy. This method, which is simple and cost-effective, has great potential for clinical cancer diagnosis as a liquid biopsy technique.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemical Research Methods
Kunpeng Cai, Shruti Mankar, Taiga Ajiri, Kentaro Shirai, Tasuku Yotoriyama
Summary: The study introduces a fully integrated high-throughput microfluidic circulatory fluorescence-activated cell sorting system capable of significantly increasing the purity of rare cells in a short period of time and effectively enriching different types of cells. Through a sequential sorting process and efficient contamination-free design, effective enrichment of clinical rare cells has been achieved.
Article
Engineering, Electrical & Electronic
Arda Secme, Uzay Tefek, Burak Sari, Hadi Sedaghat Pisheh, H. Dilara Uslu, Ozge Akbulut Caliskan, Berk Kucukoglu, Ramazan Tufan Erdogan, Hashim Alhmoud, Ozgur Sahin, M. Selim Hanay
Summary: Microwave sensors combined with optical sensing can be used to probe material properties and track size changes of microscale objects. In this study, we designed and fabricated two types of planar sensors, the CPW resonator and the SRR, to detect single cells and particles. The results show that microwave sensing technology can serve as a complementary technique for single-cell biophysical experiments and microscale pollutant screening.
IEEE SENSORS JOURNAL
(2023)
Article
Optics
Dong-Yang Li, Han Zhang, Zhi Li, Ling-Wei Zhou, Meng-Da Zhang, Xiao-Yun Pu, Yu-Ze Sun, Hang Liu, Yuan-Xian Zhang
Summary: We present a chip-scale integrated pH sensor using an optofluidic ring resonator (OFRR) laser, which exhibits high sensitivity and linearity in the pH range 6.51-8.13. The pumping scheme provides uniform excitation to the gain medium, resulting in a low lasing threshold and highly sensitive sensing. This sensing platform has excellent robustness and low sample consumption, making it suitable for label-free, real-time, and in situ detection in medicine and hazardous/toxic/volatile sensing applications.
Article
Chemistry, Analytical
Yoo Min Park, Juhwan Park, Sun Young Lim, Yeji Kwon, Nam Ho Bae, Je-Kyun Park, Seok Jae Lee
Summary: The study developed a simple single-chip pathogen analysis system by integrating different modules for gene amplification, electrochemical analysis, and sample migration and mixing without external pumping systems. The system successfully detected target pathogens in laboratory conditions, demonstrating practicality and sensitivity.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Lijiao Zu, Yu Chen, Jiwei Xie, Weiru Liu, Yue Feng, Zhaochuan Zhang, Xiujuan Zhao, Yan Ma, Qian Fang, Kaiwei Li, Tuan Guo, Mingxian Liu, Hui Wu
Summary: We propose a novel approach of using a nanotube-functionalized and microfluidic-controlling multiresonance optical fiber sensor for in situ and highly sensitive monitoring of tumor cells. The sensor can rapidly capture and precisely quantify tumor cells, enabling unambiguous discrimination between normal and tumor cells within a few minutes based on the spectral area interrogation method. It provides high sensitivity and a linear response for a large cell concentration range.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Amin Dehghan, Ali Gholizadeh, Mahdi Navidbakhsh, Hossein Sadeghi, Esmail Pishbin
Summary: Micromixers are essential microfluidic modules for fabricating integrated lab-on-chip devices for point-of-care applications. In this study, a magnetic stirrer on a rotating micro-structured disk is presented to mix liquids of a wide range of viscosities in low rotational velocities. The on-disk magnetic stirrer can be implemented in integrated micmfluidic platforms without affecting other modules, and it is a convenient choice for the cell lysis process due to high mixing indexes and considerable viscous stresses.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Plant Sciences
Ken-ichi Kurotani, Yaichi Kawakatsu, Masahiro Kikkawa, Ryo Tabata, Daisuke Kurihara, Hiroyuki Honda, Kazunori Shimizu, Michitaka Notaguchi
Summary: Researchers used tobacco BY-2 cells and a microfluidic device to monitor the behavior of plasmodesmata, finding that recovery of H2B-GFP protein was mainly due to translocation from neighboring cells rather than de novo protein synthesis, and that sodium chloride inhibited the transport of H2B-GFP protein.
JOURNAL OF PLANT RESEARCH
(2022)
Article
Optics
Chunlei Jiang, Yunkai Wang, Taiji Dong, Dong Li, Bing Yan, Peng Chen, Keyong Shao, Xiufang Wang, Zengbo Wang
Summary: A completely non-invasive method for cell manipulation is proposed by combining a microcapillary microfluidic device and an optical fiber tweezer. Chlorella cells inside the microcapillary channel are successfully trapped and manipulated by an optical fiber probe located outside of the microcapillary, making the process completely non-invasive. The curved walls of the microcapillaries act as a lens to enhance light focusing and trapping efficiency.
Article
Chemistry, Analytical
Kai Zhao, Penglu Zhao, Jianhong Dong, Yunman Wei, Bin Chen, Yanjuan Wang, Xinxiang Pan, Junsheng Wang
Summary: This study demonstrates the successful isolation of circulating tumor cells (CTCs) from blood cells using a combination of dielectrophoresis and magnetophoresis in a microfluidic chip. The results show the potential application of this method in diagnostics.
Article
Chemistry, Analytical
Sebastian K. Piendl, David Geissler, Laura Weigelt, Detlev Belder
ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Rico Warias, Daniele Ragno, Alessandro Massi, Detlev Belder
CHEMISTRY-A EUROPEAN JOURNAL
(2020)
Article
Biochemical Research Methods
Benjamin Krafft, Anna Tycova, Raphael D. Urban, Christian Dusny, Detlev Belder
Summary: This study introduces a disposable and low-cost lab-on-a-chip device utilizing a nanoporous membrane for the identification of bacterial pathogens in drinking water. The device showed promising results in rapid and reliable pathogen detection, making it suitable for common pathogens in spiked tap water.
Article
Chemistry, Analytical
Martin Schirmer, Konstantin Wink, Stefan Ohla, Detlev Belder, Andreas Schmid, Christian Dusny
ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Physical
Liliana Calzadiaz-Ramirez, Carla Calvo-Tusell, Gabriele M. M. Stoffel, Steffen N. Lindner, Silvia Osuna, Tobias J. Erb, Marc Garcia-Borras, Arren Bar-Even, Carlos G. Acevedo-Rocha
Article
Biochemical Research Methods
Raphael D. Urban, Tillmann G. Fischer, Ales Charvat, Konstantin Wink, Benjamin Krafft, Stefan Ohla, Kirsten Zeitler, Bernd Abel, Detlev Belder
Summary: This approach involves generating droplets of non-polar solvents in an aqueous matrix and ionizing the solutes with an IR laser for mass spectrometric analysis at atmospheric pressure. It works for both non-polar solvents such as n-heptane and water non-miscible solvents like chloroform. It has been successfully applied to monitor the photooxidation of N-phenyl-1,2,3,4-tetrahydroisoquinoline in a proof of concept study.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Physical
Carlos G. Acevedo-Rocha, Frank Hollmann, Joaquin Sanchis, Zhoutong Sun
Article
Biochemical Research Methods
Sadat Hasan, Maximilian E. Blaha, Sebastian K. Piendl, Anish Das, David Geissler, Detlev Belder
Summary: This research introduces a label-free detection technique using time-resolved two-photon excitation (TPE) fluorescence detection at 532 nm in droplet microfluidics. A polydimethylsiloxane-fused silica (FS) hybrid chip was developed to provide high optical transparency in the deep-UV range and suitable surface properties for droplet microfluidics. The system incorporating a 532-nm picosecond laser, time-correlated single-photon counting (TCSPC), and a chip-integrated dielectrophoretic pulsed actuator was utilized to sort droplets containing specific compounds and screen yeast cells based on their protein autofluorescence.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Nora T. Hartner, Konstantin Wink, Christian-Robert Raddatz, Christian Thoben, Martin Schirmer, Stefan Zimmermann, Detlev Belder
Summary: The coupling of droplet microfluidics and high-resolution drift tube IMS system enables chemical-specific monitoring of reactions in droplets at single droplet level, achieving limit of detections down to 200 nM to 1 µM. This approach is cost-effective and robust, making it ideal for chemical-specific end-of-line detection for segmented flow experiments.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Konstantin Wink, Marie van Der Loh, Nora Hartner, Matthias Polack, Christian Dusny, Andreas Schmid, Detlev Belder
Summary: Chemically specific and quantifiable monitoring of biocatalytic transformations at the single-cell level has been achieved through the integration of droplet microfluidics, cell imaging, and mass spectrometry.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Analytical
Anish Das, Sebastian Fehse, Matthias Polack, Rajapandiyan Panneerselvam, Detlev Belder
Summary: We present a new approach for surface-enhanced Raman spectroscopy (SERS) detection in digital microfluidics (DMF) using a microspray hole (mu SH) that utilizes electrostatic spray (ESTAS) for sample transfer. A novel ESTAS-compatible stationary SERS substrate was developed and characterized for sensitive and reproducible SERS measurements. Proof-of-concept experiments showed successful detection of various analyte molecules with micro-molar detection limits using the DMF chip and provided vibrational spectroscopic data of an organic reaction occurring in the DMF device.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Tobias Haensch, Franziska D. Zitzmann, Heinz-Georg Jahnke, Maximilian E. Blaha, Erik Paternoga, Kirsten Zeitler, Detlev Belder, Andrea A. Robitzki
Summary: This article introduces the miniaturization and integration of chemical reactions into fluidic systems to reduce solvent and reactant usage while increasing synthesis efficiency. Real-time, label-free monitoring is achieved through electrochemical impedance spectroscopy. The optimization of electrode structures in microfluidic channels allows for sensitive detection and quantification of compounds like propranolol. The integrated electrodes also enable the monitoring and control of fluidic flows for purification processes during free-flow electrophoresis.
Article
Chemistry, Multidisciplinary
Hannes Westphal, Rico Warias, Chris Weise, Daniele Ragno, Holger Becker, Matthias Spanka, Alessandro Massi, Roger Glaser, Christoph Schneider, Detlev Belder
Summary: A highly integrated microfluidic device is developed for parallel study of stereoselective heterogeneous catalytic processes. It combines multiple packed-bed mu-flow reactors with a chiral separation unit on a chip, seamlessly connected to mass spectrometry. The device allows automated fluidic setup and enables the study of different catalysts' performance using small amounts of resources.
REACTION CHEMISTRY & ENGINEERING
(2022)
