Laccase engineering by rational and evolutionary design

Construction of a Laccase Chimerical Gene: Recombinant Protein Characterization and Gene Expression via Yeast Surface Display

(2014) G. Bleve et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Crystal structure of the CueO mutants at Glu506, the key amino acid located in the proton transfer pathway for dioxygen reduction

(2013) Hirofumi Komori et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

New colorimetric screening assays for the directed evolution of fungal laccases to improve the conversion of plant biomass

(2013) Isabel Pardo et al.   BMC BIOTECHNOLOGY

Widening the pH Activity Profile of a Fungal Laccase by Directed Evolution

(2013) Pamela Torres-Salas et al.   CHEMBIOCHEM

Concerted Electron/Proton Transfer Mechanism in the Oxidation of Phenols by Laccase

(2013) Carlo Galli et al.   CHEMBIOCHEM

Optimization of a Small Laccase by Active-Site Redesign

(2013) Miguel D. Toscano et al.   CHEMBIOCHEM

Blood Tolerant Laccase by Directed Evolution

(2013) Diana M. Mate et al.   CHEMISTRY & BIOLOGY

Enhancement of catalysis and functional expression of a bacterial laccase by single amino acid replacement

(2013) Nikoo Nasoohi et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Setting the stage for electron transfer: Molecular basis of ABTS-binding to four laccases from Trametes versicolor at variable pH and protein oxidation state

(2013) Niels J. Christensen et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

The role of Asp116 in the reductive cleavage of dioxygen to water in CotA laccase: assistance during the proton-transfer mechanism

(2012) Catarina S. Silva et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Switching from blue to yellow: altering the spectral properties of a high redox potential laccase by directed evolution

(2012) Diana M. Mate et al.   BIOCATALYSIS AND BIOTRANSFORMATION

Development of chimeric laccases by directed evolution

(2012) Isabel Pardo et al.   BIOTECHNOLOGY AND BIOENGINEERING

The kinetic role of carboxylate residues in the proximity of the trinuclear centre in the O2 reactivity of CotA-laccase

(2012) Vânia Brissos et al.   DALTON TRANSACTIONS

Docking Simulation and Competitive Experiments Validate the Interaction Between the 2,5-Xylidine Inhibitor andRigidoporus lignosusLaccase

(2012) Maria Teresa Cambria et al.   JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS

In silico study of structural determinants modulating the redox potential ofRigidoporus lignosusand other fungal laccases

(2012) Maria Teresa Cambria et al.   JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS

Involvement of Tyr108 in the Enzyme Mechanism of the Small Laccase from Streptomyces coelicolor

(2012) Ankur Gupta et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Role of the C-terminus of Pleurotus eryngii Ery4 laccase in determining enzyme structure, catalytic properties and stability

(2012) G. Bleve et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Engineering Platforms for Directed Evolution of Laccase from Pycnoporus cinnabarinus

(2011) S. Camarero et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

How is the reactivity of laccase affected by single-point mutations? Engineering laccase for improved activity towards sterically demanding substrates

(2011) Carlo Galli et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Crystal structure of an ascomycete fungal laccase from Thielavia arenaria - common structural features of asco-laccases

(2011) Juha P. Kallio et al.   FEBS Journal

Design Parameters for Tuning the Type 1 Cu Multicopper Oxidase Redox Potential: Insight from a Combination of First Principles and Empirical Molecular Dynamics Simulations

(2011) Gongyi Hong et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Laccases and their natural mediators: Biotechnological tools for sustainable eco-friendly processes

(2010) Ana I. Cañas et al.   BIOTECHNOLOGY ADVANCES

Laboratory Evolution of High-Redox Potential Laccases

(2010) Diana Maté et al.   CHEMISTRY & BIOLOGY

The role of Glu498 in the dioxygen reactivity of CotA-laccase from Bacillus subtilis

(2010) Zhenjia Chen et al.   DALTON TRANSACTIONS

Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast

(2010) Eva García-Ruiz et al.   Microbial Cell Factories

A Semi-Rational Approach to Engineering Laccase Enzymes

(2010) Annalisa Miele et al.   MOLECULAR BIOTECHNOLOGY

Improving the functional expression of a Bacillus licheniformis laccase by random and site-directed mutagenesis

(2009) Katja Koschorreck et al.   BMC BIOTECHNOLOGY

Molecular determinants of peculiar properties of a Pleurotus ostreatus laccase: Analysis by site-directed mutagenesis

(2009) Flavia Autore et al.   ENZYME AND MICROBIAL TECHNOLOGY

Plasticity of laccase generated by homeologous recombination in yeast

(2009) Angela M. Cusano et al.   FEBS Journal

Essential role of the C-terminus in Melanocarpus  albomyces laccase for enzyme production, catalytic properties and structure

(2009) Martina Andberg et al.   FEBS Journal

Random mutants of aPleurotus ostreatuslaccase as new biocatalysts for industrial effluents bioremediation

(2009) A. Miele et al.   JOURNAL OF APPLIED MICROBIOLOGY

Four-electron Reduction of Dioxygen by a Multicopper Oxidase, CueO, and Roles of Asp112and Glu506Located Adjacent to the Trinuclear Copper Center

(2009) Kunishige Kataoka et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure–Function Studies of a Melanocarpus albomyces Laccase Suggest a Pathway for Oxidation of Phenolic Compounds

(2009) J.P. Kallio et al.   JOURNAL OF MOLECULAR BIOLOGY

Laboratory evolution of laccase for substrate specificity

(2009) Nirupama Gupta et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

A chimeric laccase with hybrid properties of the parental Lentinula edodes laccases

(2009) Yuko Nakagawa et al.   MICROBIOLOGICAL RESEARCH

Exploring protein fitness landscapes by directed evolution

(2009) Philip A. Romero et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Molecular cloning and heterologous expression of a laccase gene from Pleurotus eryngii in free and immobilized Saccharomyces cerevisiae cells

(2008) Gianluca Bleve et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Comparative characterization of four laccases from Trametes versicolor concerning phenolic C–C coupling and oxidation of PAHs

(2008) Katja Koschorreck et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Spectroscopic Studies of Perturbed T1 Cu Sites in the Multicopper OxidasesSaccharomyces cerevisiaeFet3p andRhus verniciferaLaccase:  Allosteric Coupling between the T1 and Trinuclear Cu Sites†

(2008) Anthony J. Augustine et al.   BIOCHEMISTRY

Combinatorial Saturation Mutagenesis of the Myceliophthora thermophila Laccase T2 Mutant: the Connection between the C-Terminal Plug and the Conserved VSG Tripeptide

(2008) Miren Zumarraga et al.   COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING

Direct Electrochemistry of CueO and Its Mutants at Residues to and Near Type I Cu for Oxygen-Reducing Biocathode

(2008) Y. Miura et al.   Fuel Cells

Crystal structure of the blue multicopper oxidase from the white-rot fungus Trametes trogii complexed with p-toluate

(2008) Irene Matera et al.   INORGANICA CHIMICA ACTA

The Structure of the Small Laccase from Streptomyces coelicolor Reveals a Link between Laccases and Nitrite Reductases

(2008) Tereza Skálová et al.   JOURNAL OF MOLECULAR BIOLOGY

An assessment of the relative contributions of redox and steric issues to laccase specificity towards putative substrates

(2008) Mahelet Aweke Tadesse et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Development of new laccases by directed evolution: Functional and computational analyses

(2008) Giovanna Festa et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Altering the laccase functionality byin vivo assembly of mutant libraries with different mutational spectra

(2007) Miren Zumárraga et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS