Article
Immunology
Kazuhiro Ogi, Tetsuji Takabayashi, Kaori Tomita, Masafumi Sakashita, Taiyo Morikawa, Takahiro Ninomiya, Masayuki Okamoto, Norihiko Narita, Shigeharu Fujieda
Summary: The study found that ORMDL3 plays an important role in regulating proinflammatory cytokine expression via the SIT pathway and selectively affects the UPR pathway in mast cells.
IMMUNITY INFLAMMATION AND DISEASE
(2021)
Review
Cell Biology
Joanna Baran, Anna Sobiepanek, Anna Mazurkiewicz-Pisarek, Marta Rogalska, Aleksander Gryciuk, Lukasz Kuryk, Soman N. Abraham, Monika Staniszewska
Summary: Mast cells are immune cells found in various tissues and their overactivity can lead to health hazards. They can cause allergic reactions and play a role in tumor biology. However, the mechanisms of mast cell actions are still poorly understood, making it challenging to develop therapies for their pathological condition.
Review
Immunology
Peter W. West, Silvia Bulfone-Paus
Summary: Mast cells exhibit heterogeneity in different tissues and their interactions with immune cells may be regulated by tissue-specific factors.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Theres Klewer, Ljubica Bakic, Thomas Mueller-Reichert, Robert Kiewisz, Gregor Jessberger, Nadine Kiessling, Axel Roers, Rolf Jessberger
Summary: The study shows that E-Cadherin plays a role in restricting premature activation of mast cells, with in vitro and in vivo experiments confirming this conclusion. A deficiency of E-Cadherin in mast cells leads to enhanced degranulation and decreased release of newly synthesized inflammatory cytokines, indicating a stronger response to IgE-dependent stimuli and increased risk of anaphylactic shock.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Remo Poto, Vincenzo Patella, Gjada Criscuolo, Gianni Marone, Enrico Coscioni, Gilda Varricchi
Summary: Mast cells have complex functions in tissue homeostasis and disease. Cardiac mast cells express the high-affinity receptor for IgE and can be activated by anti-IgE. The presence of functional anti-IgE autoantibodies can strongly activate mast cells to release proinflammatory and vasoactive mediators.
CLINICAL AND EXPERIMENTAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Jagdeep K. Sandhu, Marianna Kulka
Summary: Microglia and mast cells are important immune cells in the central nervous system, playing key roles in immune surveillance, neuroinflammation, and neurodegenerative diseases. They communicate through mediators and receptors, coordinating immune responses, but dysregulated activation can lead to neuronal dysfunction and death. Targeting common pathways in mast cells and microglia may offer potential therapeutic approaches to control inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Ashley Wagner, Syed Benazir Alam, Marianna Kulka
Summary: This study compared the response of different mast cell models to common stimuli and found that factors such as source, age, and biological sex play an important role in phenotypic outcomes and may explain the variability in their function.
CELLULAR IMMUNOLOGY
(2023)
Article
Cell Biology
Lakshmi Bhargavi Paruchuru, Sharmila Govindaraj, Ehud Razin
Summary: Recent studies have revealed the crucial role of mitochondria in mast cell immunological activation. This study demonstrates that the phosphorylation of Serine 73 on mitochondrial MITF plays a significant role in regulating mast cell degranulation and cytokine secretion.
Article
Medicine, Research & Experimental
E. S. Trofimova, M. V. Zykova, E. Yu. Sherstoboev, M. G. Danilets, A. A. Ligacheva, M. V. Belousov
Summary: The administration of humic acids isolated with sodium pyrophosphate from raised bog sphagnum peat reduces Th1-type immune response and the severity of anaphylactic shock in mice. It also decreases mast cell degranulation after immunization with ovalbumin. Additionally, the addition of humic acids increases the production of IL-4, IL-10 and reduces the production of IL-2, IFN gamma by peripheral blood mononuclear cells of healthy donors.
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
(2022)
Article
Allergy
Shunli Pan, Eujin Lee, Youn Ju Lee, Meihua Jin, Eunkyung Lee
Summary: The study demonstrated that tamarixetin effectively inhibits the production of inflammatory mediators in BMMCs and reduces degranulation. It exerts its effects through the phospholipase C-γ 1 and Akt signaling pathways.
ALLERGOLOGIA ET IMMUNOPATHOLOGIA
(2021)
Article
Allergy
Shunli Pan, Eujin Lee, Youn Ju Lee, Meihua Jin, Eunkyung Lee
Summary: The study demonstrates that tamarixetin effectively inhibits the production of inflammatory mediators in mouse BMMCs by suppressing the phosphorylation of various signaling molecules.
ALLERGOLOGIA ET IMMUNOPATHOLOGIA
(2021)
Review
Immunology
Zane Orinska, Philipp M. Hagemann, Ivana Halova, Petr Draber
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(2020)
Article
Chemistry, Physical
Yoshihisa Okuchi, Joshua Reeves, Soon Seng Ng, Daniel H. Doro, Sergi Junyent, Karen J. Liu, Alicia J. El Haj, Shukry J. Habib
Summary: The Wnt3a protein immobilized on a biocompatible bandage induces directed asymmetric cell division of human skeletal stem cells and promotes bone tissue repair. The transplantation of a bandage containing a Wnt-induced osteogenic tissue model facilitates long-term viability of hSSCs and their progeny, resulting in bone repair in a mouse model of critical-sized calvarial defects. This approach provides insights into human osteogenesis regulation and offers a promising method for delivering human osteogenic constructs for in vivo survival and contribution to bone repair.
Article
Cell Biology
Sergi Junyent, Joshua Reeves, Eileen Gentleman, Shukry J. Habib
Summary: The study reveals that the pairing and signal transduction mechanisms of stem cells with other cells vary with different pluripotency states, affecting their ability for self-organization and synthetic embryogenesis.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Monika Bambouskova, Lucie Potuckova, Tomas Paulenda, Martina Kerndl, Denis A. Mogilenko, Kate Lizotte, Amanda Swain, Sebastian Hayes, Ryan D. Sheldon, Hyeryun Kim, Unnati Kapadnis, Abigail E. Ellis, Christine Isaguirre, Samantha Burdess, Anwesha Laha, Gaya K. Amarasinghe, Victor Chubukov, Thomas P. Roddy, Michael S. Diamond, Russell G. Jones, Donald M. Simons, Maxim N. Artyomov
Summary: Itaconate, induced upon TLR stimulation, regulates NLRP3 inflammasome activation and synergizes with iNOS, establishing tolerance to NLRP3 inflammasome in inflammatory macrophages. Mechanistically, itaconate prevents full caspase-1 activation and gasdermin D processing, potentially leading to pyroptotic cell death and tissue damage if not controlled.
Article
Immunology
Viktor Bugajev, Ivana Halova, Livia Demkova, Sara Cernohouzova, Petra Vavrova, Michal Mrkacek, Pavol Utekal, Lubica Draberova, Ladislav Kuchar, Bjorn Schuster, Petr Draber
Summary: The study revealed that ORMDL2 and ORMDL3 proteins play important roles in mast cell signaling, with double KO leading to increased intracellular levels of sphingosine-1-phosphate and enhanced cellular responses and cytokine production. In vitro experiments demonstrated increased chemotaxis in antigen-activated cells, while in vivo experiments showed an increase in passive cutaneous anaphylaxis in double KO mice, but faster recovery.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Viktor Bugajev, Tomas Paulenda, Pavol Utekal, Michal Mrkacek, Ivana Halova, Ladislav Kuchar, Ondrej Kuda, Petra Vavrova, Bjorn Schuster, Sergio Fuentes-Liso, Lucie Potuckova, Daniel Smrz, Sara Cernohouzova, Lubica Draberova, Monika Bambouskova, Petr Draber
Summary: The study revealed the critical role of ORMDL3 in regulating the synthesis of sphingolipids and leukotrienes by examining peritoneal-derived mast cells from ORMDL3 KO and control mice. ORMDL3-deficient cells not only produced more sphingolipids but also showed increased production of LT signaling mediators. Furthermore, functional crosstalk between ORMDL3 and 5-LO metabolic pathways was demonstrated.
JOURNAL OF LIPID RESEARCH
(2021)
Article
Biology
Sergi Junyent, Joshua C. Reeves, James L. A. Szczerkowski, Clare L. Garcin, Tung-Jui Trieu, Matthew Wilson, Jethro Lundie-Brown, Shukry J. Habib
Summary: The Wnt-pathway interacts with AMPA/Kainate-type ionotropic glutamate receptors during cell recruitment, dynamics, and spindle orientation. AMPA receptors are crucial for segregating cell fate components in asymmetric cell division. The Wnt co-receptor Lrp6 plays a specific role in cytoneme formation and ACD, along with Lrp5 and β-catenin.
Review
Immunology
Lubica Draberova, Magda Tumova, Petr Draber
Summary: Mast cells are potent immune sensors that release preformed biologically active products and initiate cytokine synthesis upon activation. The activation pathways are regulated at multiple levels, with a critical pathway involving pore-forming bacterial exotoxins that are cholesterol-dependent.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Gouse M. Shaik, Lubica Draberova, Sara Cernohouzova, Magda Tumova, Viktor Bugajev, Petr Draber
Summary: The bioactive compound ursolic acid (UA) has anti-inflammatory effects and can inhibit mast cell response, calcium uptake, and migration. It enhances the production of certain cytokines and affects the phosphorylation of signaling proteins. UA also alters the properties of plasma membrane microdomains, affecting the function of the FceRI signalosome.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Livia Demkova, Viktor Bugajev, Pavol Utekal, Ladislav Kuchar, Bjorn Schuster, Petr Draber, Ivana Halova
Summary: In mammals, the ORMDL family consists of three highly homologous members, ORMDL1, ORMDL2, and ORMDL3. ORMDL3 has been associated with childhood-onset asthma and other inflammatory diseases in which mast cells play a role. Deletion of ORMDL1 and ORMDL3 enhances mast cell activation and cytokine production, with ORMDL3 expression levels being crucial for the pro-inflammatory phenotype.
SCIENTIFIC REPORTS
(2023)
Article
Biochemical Research Methods
Sergi Junyent, Joshua Reeves, Shukry J. Habib
Summary: These protocols examine the interaction of cytonemes with localized Wnt and can be used to analyze cell-niche signaling between ESCs and TSCs or Wnt signals tethered to microbeads in vitro. The pairing interaction between ESCs and Wnt-sources is crucial for synthetic embryogenesis, making this protocol effective for developmental biology studies in vitro.