Article
Biochemistry & Molecular Biology
Seongjun So, Yoonkyung Park, Soon Suk Kang, Jongsuk Han, Jeong Hye Sunwoo, Whanseo Lee, Jin Kim, Eun Ah Ye, Jae Yong Kim, Hungwon Tchah, Eunju Kang, Hun Lee
Summary: Corneal endothelial cells (CECs) cannot proliferate or recover, causing decreased cell density and loss of pump/barrier function. The study investigated the efficacy and safety of transplanting induced pluripotent stem cell (iPSC)-derived CECs into a corneal endothelial dysfunction (CED) rabbit model. Results showed successful differentiation of iPSCs into CECs and improved corneal transparency after transplantation. iPSC-derived CECs may be a promising cellular resource for treating CED.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Peng Sun, Lin Shen, Yuan-Bin Li, Li-Qun Du, Xin-Yi Wu
Summary: In this study, human corneal endothelial cells (HCECs) were cultivated using a conditioned medium from orbital adipose-derived stem cells (OASC-CM) and then transplanted into animal models with corneal endothelial dysfunction. Long-term observation revealed successful outcomes, with transparent corneas and maintained cell morphology. This research demonstrates a promising prospect for cell-based therapy for corneal endothelial diseases.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Nanoscience & Nanotechnology
Zhiwei Jia, Shunxin Zhang, Wei Li
Summary: Degenerative bone disorders such as IVDD, OA, and OP present significant challenges for aging populations with no effective treatments available. The use of stem cell-derived EVs as a novel therapeutic approach holds promise in addressing these disorders. However, a comprehensive assessment of EV-based therapies in degenerative bone disorders is currently lacking. This review aims to fill this gap by summarizing the current state of the field and discussing the potential of EV-based treatments for IVDD, OA, and OP.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Review
Medicine, Research & Experimental
Mengyuan Wang, Ying Li, Hongqiao Wang, Meng Li, Xiaomin Wang, Rongzhen Liu, Daijun Zhang, Wenhua Xu
Summary: Corneal epithelial defects and excessive wound healing can cause severe complications. Stem cells offer a promising solution for regenerating the corneal epithelium and treating severe corneal epithelial injury. Biological scaffolds such as the amniotic membrane, fibrin, and hydrogels provide the necessary signals for stem cell proliferation and differentiation. Researchers have evaluated these scaffolds as potential therapeutic interventions for corneal disorders, identifying both benefits and drawbacks. This study provides a comprehensive overview of the history and use of stem cells in corneal repair, focusing on biological scaffolds used in stem cell transplantation and innovative materials under investigation.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Cell Biology
Jorge L. Alio Del Barrio, Ana De la Mata, Maria P. De Miguel, Francisco Arnalich-Montiel, Teresa Nieto-Miguel, Mona El Zarif, Marta Cadenas-Martin, Marina Lopez-Paniagua, Sara Galindo, Margarita Calonge, Jorge L. Alio
Summary: Adipose-derived stem cells have the advantages of easy accessibility, multipotency, and tissue regeneration potential, making them a potential source for cell-free regenerative therapy, particularly for the regeneration of the human cornea.
Review
Pharmacology & Pharmacy
Lu Zhang, Xiao-Jing-Nan Ma, Yuan-Yuan Fei, Heng-Tong Han, Jun Xu, Lu Cheng, Xun Li
Summary: The liver has the ability to repair itself, but pathological changes can impair its regenerative capacity and lead to liver failure. Mesenchymal stem cells (MSCs) have shown promise in liver regeneration, and induced pluripotent stem cells have made progress in organoid establishment and drug screening.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Juan Yu, Nianye Yu, Yao Tian, Yifan Fang, Bin An, Guihai Feng, Jun Wu, Liu Wang, Jie Hao, Liqiang Wang, Qi Zhou, Wei Li, Yukai Wang, Baoyang Hu
Summary: Our study presents an improved protocol for differentiating clinical-grade hESCs into iCECs using xeno-free medium and cryopreservation. The generated iCECs express typical CECs markers and exhibit transendothelial potential properties. After rigorous quality control, the cells meeting release criteria were available for in vivo studies. Grafts of iCECs did not show overgrowth or tumorigenicity in immunodeficient mice and successfully ameliorated edema and corneal opacity in rabbit models.
CELL AND BIOSCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Jie Chen, Qingjian Ou, Zhe Wang, Yifan Liu, Shuqin Hu, Yumeilan Liu, Haibin Tian, Jingying Xu, Furong Gao, Lixia Lu, Caixia Jin, Guo-Tong Xu, Hong-Ping Cui
Summary: The study developed an effective method using small molecules to differentiate hiPSCs into CECs, promoting the conversion of NCCs to CECs by upregulating key signaling pathways and factors.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Cell & Tissue Engineering
Sara Galindo, Ana de la Mata, Marina Lopez-Paniagua, Jose M. Herreras, Inmaculada Perez, Margarita Calonge, Teresa Nieto-Miguel
Summary: Mesenchymal stem cells (MSCs) have unique properties that make them effective for treating ocular surface diseases. The most commonly used administration routes for MSC treatment of Limbal Stem Cell Deficiency (LSCD) include intravenous and intraperitoneal injections, topical administration, and MSC transplantation using carrier substrata like amniotic membrane (AM). Subconjunctival injection is a minimally invasive technique that is frequently used in ophthalmology to treat corneal failure due to LSCD.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Ophthalmology
Koji Kitazawa, Chie Sotozono, Shigeru Kinoshita
Summary: Corneal epithelial stem cells (CEpSCs) are responsible for tissue homeostasis in the limbal area. Cell-based therapy using CEpSCs has shown successful outcomes for treating complete CEpSC deficiency. Significant advancements have been made in both in vivo and ex vivo cell-based ocular surface reconstruction therapies over the past few decades. New therapeutic concepts and strategies are emerging for corneal endothelial dysfunction treatment.
ASIA-PACIFIC JOURNAL OF OPHTHALMOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Xiao Yu Ng, Gary S. L. Peh, Gary Hin-Fai Yam, Hwee Goon Tay, Jodhbir S. Mehta
Summary: Corneal endothelial dysfunction is a major cause of corneal blindness, and the current treatment option is corneal transplantation. However, there is a shortage of suitable donor graft material, leading to the exploration of stem cell-based regenerative medicine using induced pluripotent stem cells (iPSCs). This review discusses the progress and challenges in inducing iPSCs into corneal endothelial cells (CECs), as well as the potential risks and solutions for clinical application.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Eun Ah Ye, Ho Seok Chung, Yoonkyung Park, Jeong Hye Sunwoo, Whanseo Lee, Jin Kim, Hungwon Tchah, Hun Lee, Jae Yong Kim
Summary: This study demonstrated the differentiation of human umbilical cord-derived mesenchymal stem cells (UC-MSCs) into corneal endothelial cells (CECs). The efficacy of MSC-induced CEC injection was investigated in a rabbit model of corneal endothelial dysfunction (CED). The results showed that MSC-induced CEC injection improved corneal opacity and neovascularization in the rabbit model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Bojiao Tang, Xiaoling Xie, Ruhui Yang, Sijie Zhou, Ronggui Hu, Jiayao Feng, Qinxiang Zheng, Xingjie Zan
Summary: The hydrophobicity of nanoparticles plays a significant role in their ability to penetrate biological barriers. This study successfully encapsulated the protein drug Ava by decorating hydrophobic amino acid tyrosine (Y) onto a peptide chain, resulting in improved bioavailability and therapeutic outcomes.
ACTA BIOMATERIALIA
(2022)
Review
Polymer Science
Martine Tarsitano, Maria Chiara Cristiano, Massimo Fresta, Donatella Paolino, Concetta Rafaniello
Summary: Corneal transplantation has been the preferred treatment for irreversible damage to the anterior part of the eye. However, limited cornea donors and cases of graft rejection have led to the exploration of new biomaterials. Tissue engineering is crucial in developing a construct that mimics all the necessary properties of the cornea for proper vision. This review discusses the anatomy and physiology of the cornea, the roles of different corneal layers, and the requirements for manufacturing materials. Alginate has emerged as a promising candidate for corneal regeneration due to its adaptability, transparency, and low immunogenicity. Chemical modifications and blending with other compounds can further enhance its mechanical properties and cell-proliferation features.
Review
Biochemistry & Molecular Biology
Basanta Bhujel, Se-Heon Oh, Chang-Min Kim, Ye-Ji Yoon, Young-Jae Kim, Ho-Seok Chung, Eun-Ah Ye, Hun Lee, Jae-Yong Kim
Summary: The cornea is susceptible to damage, but corneal transplantation can restore its functions. However, this procedure faces obstacles such as a shortage of donors and graft rejection. Mesenchymal stem cells (MSCs) have shown potential in corneal regeneration by differentiating into corneal cells and secreting growth factors. This review highlights the contribution of MSCs and MSC-derived exosomes in overcoming corneal disease and their potential as therapeutic agents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Applied
Ching-Yao Tsai, Lin-Chung Woung, Jiin-Cherng Yen, Po-Chen Tseng, Shih-Hwa Chiou, Yen-Jen Sung, Kuan-Ting Liu, Yung-Hsin Cheng
CARBOHYDRATE POLYMERS
(2016)
Article
Medicine, General & Internal
Chian-Feng Chen, Shiow-Wen Liou, Hsin-Han Wu, Chin-Hui Lin, Li-Shan Huang, Lin-Chung Woung, Ching-Yao Tsai
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2016)
Letter
Health Care Sciences & Services
Sheng-Jean Huang, Samuel Shih-Chih Wang, Oscar Kuang-Sheng Lee, Lin-Chung Woung, Da-Chen Chu, Tsun-Cheng Huang, Chih-Kuang Liu, Sheng-Huang Hsiao, Hung-Meng Huang, Mu-Jung Kao, Winnie Yang, Tien Wei Yang, Chung-Hua Hsu, Lin-Yang Chi, Li-Lin Kuo, Yung-Sen Chang, Ting-Fang Chiu, Ming-Chyi Huang, J. Randall Curtis
JOURNAL OF PALLIATIVE MEDICINE
(2016)
Article
Medicine, General & Internal
Chih-Chien Hsu, Nicole Huang, Pei-Yu Lin, Der-Chong Tsai, Ching-Yao Tsai, Lin-Chung Woung, Catherine Jui-Ling Liu
JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
(2016)
Editorial Material
Clinical Neurology
Hui-Chen Cheng, Tzu-Lun Huang, Ko-Jo Lin, Rong-Kung Tsai, Lin-Chung Woung, May-Yung Yen, An-Guor Wang
NEURO-OPHTHALMOLOGY
(2016)
Article
Medicine, General & Internal
Ming-Chung Ko, Sheng-Jean Huang, Chu-Chieh Chen, Yu-Ping Chang, Hsin-Yi Lien, Jia-Yi Lin, Lin-Chung Woung, Shang-Yih Chan
Letter
Health Care Sciences & Services
Sheng-Jean Huang, Chiao-Yu Huang, Lin-Chung Woung, Oscar Kuang-Sheng Lee, Da-Chen Chu, Tsun-Cheng Huang, Ying-Wei Wang, Ping Guo, Richard Harding, Allan Kellehear, J. Randall Curtis
JOURNAL OF PALLIATIVE MEDICINE
(2018)
Article
Medicine, General & Internal
Chi-Hsien Peng, Lin-Chunh Woung, Kai-Hsi Lu, Ching-Yao Tsai, Shou-Dong Lee, Chi-Shan Huang, Tai-Chi Lin, Ke-Hung Chien, De-Kuang Hwang
JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
(2018)
Article
Ophthalmology
Kuan-Hsuan Chen, En-Lin Hsiang, Min-Yen Hsu, Yueh-Ching Chou, Tai-Chi Lin, Yuh-Lih Chang, Ching-Yao Tsai, Tzu-Hsuan Li, Lin-Chung Woung, Shih-Jen Chen, Chi-Hsien Peng, De-Kuang Hwang
ACTA OPHTHALMOLOGICA
(2019)
Article
Medicine, General & Internal
Ming-Chung Ko, Hsin-Yi Lien, Lin-Chung Woung, Chin-Yi Chen, Yu-Ling Chen, Chu-Chieh Chen
JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
(2019)
Letter
Health Care Sciences & Services
Sheng-Jean Huang, Chiao-Yu Huang, Lin-Chung Woung, Oscar Kuang-Sheng Lee, Da-Chen Chu, Tsun-Cheng Huang, Akihiro Kitazawa, J. Randall Curtis
JOURNAL OF PALLIATIVE MEDICINE
(2019)
Article
Health Care Sciences & Services
Yen-Ting Ko, Ming-Chung Ko, Chao-Ming Huang, Hsin-Yi Lien, Lin-Chung Woung, Sheng-Jean Huang
JOURNAL OF PAIN AND SYMPTOM MANAGEMENT
(2020)
Review
Ophthalmology
Chao-Wen Lin, Ta-Ching Chen, Jieh-Ren Jou, Lin-Chung Woung
TAIWAN JOURNAL OF OPHTHALMOLOGY
(2018)
Article
Ophthalmology
Chih-Chien Hsu, Nicole Huang, Pei-Yu Lin, Shao-You Fang, Der-Chong Tsai, Shing-Yi Chen, Ching-Yao Tsai, Lin-Chung Woung, Shih-Hwa Chiou, Catherine Jui-ling Liu
BRITISH JOURNAL OF OPHTHALMOLOGY
(2017)
Article
Medicine, Research & Experimental
De-Kuang Hwang, Chih-Chien Hsu, Kao-Jung Chang, Daniel Chao, Chuan-Hu Sun, Ying-Chun Jheng, Aliaksandr A. Yarmishyn, Jau-Ching Wu, Ching-Yao Tsai, Mong-Lien Wang, Chi-Hsien Peng, Ke-Hung Chien, Chung-Lan Kao, Tai-Chi Lin, Lin-Chung Woung, Shih-Jen Chen, Shih-Hwa Chiou