Article
Biochemistry & Molecular Biology
Yu Yao, Jiajun Gu, Yanjiao Luo, Yixin Zhang, Yuanyue Wang, Yongzhen Pang, Shangang Jia, Chaoqun Xu, Doudou Li, Fengmei Suo, Guoan Shen, Baolin Guo
Summary: In this study, a novel gene EpF3R2 '' XylT encoding a protein related to prenylated flavonols in Epimedium pubescens was cloned and analyzed. The enzymatic activity of EpF3R2 '' XylT was examined in vitro, showing specificity towards prenylated flavonol substrates with icariin having the highest substrate affinity. The gene was found to be highly expressed in the leaves of E. pubescens, consistent with the accumulation levels of prenylated flavonols. The discovery of EpF3R2 '' XylT provides a new approach for the efficient production of prenylated flavonol trisaccharides.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Andong Yu, Xueqian Jiang, Yan Sun, Qiannan Hu, Xiaoxi Zhu, Junmei Kang, Lin Chen, Lin Liu, Linfeng Hao, Qingchuan Yang, Ruicai Long, Mingna Li
Summary: This study identified 90 UGT genes in alfalfa that are likely related to glycosylation modifications with secondary metabolites. The genes were unevenly distributed and showed specific tissue expression patterns. The study also found that these genes could be induced by phytohormones and flavonoids, indicating their involvement in secondary metabolism.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Guoan Shen, Yanjiao Luo, Yu Yao, Guoqing Meng, Yixin Zhang, Yuanyue Wang, Chaoqun Xu, Xiang Liu, Cheng Zhang, Gang Ding, Yongzhen Pang, Hui Zhang, Baolin Guo
Summary: This study achieved a chromosome-level genome assembly of Epimedium pubescens and discovered a key prenyltransferase gene and 19 potential flavonoid prenyltransferase genes. These findings are not only important for further molecular biological studies and synthetic biology research in Epimedium genus, but also provide clues for industrial production of related medicinal compounds.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Yanjie Li, Pan Li, Lei Zhang, Jing Shu, Michael H. Court, Zhuojing Sun, Lepu Jiang, Chengchao Zheng, Huairui Shu, Lusha Ji, Shizhong Zhang
Summary: In this study, the apple UGTs belonging to family 1 were identified and investigated for their involvement in plant secondary metabolism and stress tolerance. MdUGT83L3 was found to play an important role in salt and cold stress adaptation through modulating flavonoid metabolism in apple.
Article
Biochemistry & Molecular Biology
Shuangshuang Xie, Mengnan Zeng, Jingke Zhang, Juanjuan Liu, Junjun Wei, Ru Wang, Meng Li, Zhiyou Hao, Baoyu Ji, Xiaoke Zheng, Weisheng Feng
Summary: Epimesatines A-I, isolated from Epimedium sagittatum, are a group of prenylated flavonoids that exhibit anti-lung cancer activity and significant inhibition of SPHK1 expression.
Article
Agricultural Engineering
Linlin Yang, Shengwei Zhou, Yangwei Hou, Baoyu Ji, Lixin Pei, Xiuhong Su, Hua Zhong, Chengming Dong
Summary: This study investigated the effects of different light irradiations on the physiological characteristics and secondary metabolism of Epimedium sagittatum leaves. It found that red light, yellow light, and blue light had significant effects on the plant's characteristics and secondary metabolism, particularly blue light. Yellow light and blue light promoted the accumulation of flavonoids, while red light had no positive effect. Untargeted metabolome analysis confirmed the positive effects of blue light on flavonoid synthesis.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Qinghua Ye, Shiyan Zhang, Nana Qiu, Linmin Liu, Wei Wang, Qian Xie, Qiang Chang, Qingxi Chen
Summary: This study reported 140 UGTs in Chinese olive for the first time, and found that UGT84A77 may play a key role in the biosynthesis of hydrolysable tannins, providing a theoretical basis for further research on UGTs in Chinese olive.
Article
Plant Sciences
Jian Aiyou, Xu Guifang, Tian Jing
Summary: This study compared the main medicinal components in cultivated Epimedii folium with that in yield plant, finding that the quality of cultivated Epimedii folium is inferior. The medicinal chemical components in cultivated Epimedii folium are considerable, making it suitable as medicinal materials.
BANGLADESH JOURNAL OF BOTANY
(2021)
Article
Multidisciplinary Sciences
Jian Zaiyou, Tang Xiaomin, Wang Hongsheng, Xu Guifang
Summary: The study evaluated the photosynthesis of Epimedium brevicornu and found that its photosynthesis is significantly inhibited at midday. Epimedium brevicornu is a typical shade plant, adapted to very low light conditions, with a light saturation point lower than general sunlight intensity.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Applied
Juan Jiang, Bao-Guo Jiang, Bing Liu
Summary: In this experiment, a novel flavonoid compound (1) was isolated and identified from Epimedium brevicornu Maxim. This plant is widely distributed and has high medicinal value and important clinical applications.
NATURAL PRODUCT RESEARCH
(2023)
Article
Biodiversity Conservation
Ningna Yin, Zhengquan Wang, Haiyan Xiao, Tingting Lu, Naiyong Liu
Summary: This study identified and characterized the UGT gene family in the cerambycid beetle, Pharsalia antennata, and found highly conserved sequences with Anoplophora glabripennis. The UGT genes were widely expressed in the antennae and abdomens of the beetle, suggesting their roles in olfaction and detoxification.
Article
Pharmacology & Pharmacy
Yuan Gao, Wei Shi, Can Tu, Peng Li, Guanyu Zhao, Xiaohe Xiao, Jiabo Wang, Zhaofang Bai
Summary: The active constituents of Epimedii Folium enhance the activation of NLRP3 inflammasome and may be involved in the pathogenesis of drug-induced liver injury. This finding changes our conventional understanding of the relationship between immunostimulatory activity and glycogen content.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Chen Hong, Lili Yang, Yifan Zhang, Yiming Li, Huali Wu
Summary: This study demonstrates the potential pigmentation effect of Epimedium brevicornum Maxim., and elucidates its related molecular mechanisms and melanogenesis basis. Epimedium Folium extract (EFE) has been shown to exhibit repigmentation effects by promoting melanin synthesis and melanosome biogenesis/transfer. Six main flavonoid ingredients were identified among EFE, with epimedin B (EB) being confirmed as a high-content, low-toxicity, and effective melanogenic compound. These findings provide valuable insights into the pharmacology of Epimedium herb and may offer a novel therapeutic approach for hypopigmentation disorders.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Plant Sciences
Lei Ouyang, Yue Liu, Ruonan Yao, Dongli He, Liying Yan, Yuning Chen, Dongxin Huai, Zhihui Wang, Bolun Yu, Yanping Kang, Huifang Jiang, Yong Lei, Boshou Liao, Xin Wang
Summary: This study identified 267 AhUGTs clustered into 15 phylogenetic groups in the peanut genome. Group I contained the largest number of AhUGT genes and segmental duplication drove the expansion of the AhUGT gene family. Transcriptomic analysis indicated the involvement of AhUGTs in peanut growth and response to abiotic stress.
Article
Integrative & Complementary Medicine
Juntao Li, Yue Wu, Xinxin Yu, Xinyu Zheng, Jiechen Xian, Senjie Li, Wanyin Shi, Yun Tang, Zhe-Sheng Chen, Guixia Liu, Shen Yao, Jian Xu, Xiangwei Zheng
Summary: This study revealed for the first time the pharmacodynamic mechanism of 8-isopentenyl flavonoids in Epimedium sagittatum Maxim. for treating erectile dysfunction (ED), and identified several compounds with significant inhibitory activities and the ability to activate cellular signaling pathways.
Article
Multidisciplinary Sciences
Jiebing Wei, Junfeng Yang, Wenbo Jiang, Yongzhen Pang
Article
Plant Sciences
Xiaojia Su, Yaying Xia, Wenbo Jiang, Guoan Shen, Yongzhen Pang
Article
Plant Sciences
Junfeng Yang, Lin Ma, Wenbo Jiang, Yu Yao, Yuhong Tang, Yongzhen Pang
Summary: This study identified and classified 51 BGLU genes in the model legume plant Medicago truncatula, revealing their distinct expression patterns in response to various abiotic stresses and hormonal cues. The results suggest that BGLU genes play crucial roles in plant physiological processes by activating phytohormones and defense compounds in response to stressors. Further analysis indicated conserved motifs and dual targeting of MtBGLU proteins to vacuole and/or chloroplast, as well as identification of regulatory elements related to stress and phytohormones.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Genetics & Heredity
Wenbo Jiang, Lin Ma, Dengxia Yi, Yongzhen Pang
Summary: Lotus corniculatus, a member of the Fabaceae family, is an agriculturally important forage plant due to its anti-bloating properties and ability to grow in challenging soil conditions. The complete chloroplast genome of L. corniculatus was obtained in this study, revealing its genetic characteristics and close relationship with Lotus japonicas based on phylogenetic analysis.
MITOCHONDRIAL DNA PART B-RESOURCES
(2021)
Article
Genetics & Heredity
Dengxia Yi, Wenbo Jiang, Lin Ma, Yongzhen Pang
Summary: Desmodium uncinatum is an important legume forage species distributed in tropical and subtropical regions. Through our study, we obtained the complete chloroplast genome of D. uncinatum and found that it is phylogenetically closely related to the genera Glycine and Trifolium.
MITOCHONDRIAL DNA PART B-RESOURCES
(2021)
Article
Genetics & Heredity
Zhongzhiyue Jin, Wenbo Jiang, Dengxia Yi, Yongzhen Pang
Summary: The study assembled and annotated the complete chloroplast genome of sainfoin, revealing its structure and genomic characteristics. Phylogenetic analysis showed that sainfoin is most closely related to Hedysarum petrovii and Hedysarum taipeicum.
MITOCHONDRIAL DNA PART B-RESOURCES
(2021)
Article
Genetics & Heredity
Wenxuan Du, Wenbo Jiang, Dengxia Yi, Yongzhen Pang
Summary: Galega officinalis L. is a perennial herb of the Fabaceae family with colorful flowers suitable for ornamental and food purposes. Its chloroplast genome is 125,086 bp in length with a GC content of 34.18%, containing 30 tRNA, 4 rRNA, and 78 protein-coding genes. Phylogenetically, it is closely related to the genus of Cicer, Glycine, and Desmodium based on the constructed ML tree.
MITOCHONDRIAL DNA PART B-RESOURCES
(2021)
Article
Genetics & Heredity
Junfeng Yang, Wenxuan Du, Yongzhen Pang
Summary: The chloroplast genome of Agropyron desertorum was sequenced and found to be 135,459 bp in length. Phylogenetic analysis revealed a close relationship between A. desertorum and plant species of the Elymus genus.
MITOCHONDRIAL DNA PART B-RESOURCES
(2021)
Article
Genetics & Heredity
Junfeng Yang, Wenxuan Du, Yongzhen Pang
Summary: This study deciphered the complete chloroplast genome of Alopecurus pratensis, revealing its phylogenetic relationship with A. arundinaceus and providing valuable information for phylogenetic and evolutionary studies of the genus Alopecurus in the Poaceae family.
MITOCHONDRIAL DNA PART B-RESOURCES
(2021)
Article
Genetics & Heredity
Yijing Luo, Junfeng Yang, Wenxuan Du, Yongzhen Pang
Summary: In this study, the complete chloroplast genome of Agropyron pectinatum was assembled, with a length of 135,041 bp, containing 133 genes. This genome provides valuable information for variety identification and evolution studies of the Agropyron genus.
MITOCHONDRIAL DNA PART B-RESOURCES
(2021)
Article
Genetics & Heredity
Wenxuan Du, Junfeng Yang, Yongzhen Pang
Summary: The study obtained the complete chloroplast genome of Bromus biebersteinii, identified its phylogenetic relationship with other species, and successfully annotated multiple genes.
MITOCHONDRIAL DNA PART B-RESOURCES
(2021)
Article
Multidisciplinary Sciences
Qian Li, Lili Gu, Jiaxing Song, Chenjian Li, Yanhui Zhang, Yuxiang Wang, Yongzhen Pang, Bo Zhang
Summary: Medicago falcata, a leguminous forage crop, has been found to exhibit increased activities of antioxidant enzymes and soluble sugar content when challenged with PEG-6000. Transcriptome analysis revealed differential expression of 706 genes, with enrichment in various pathways including phenylpropanoid biosynthesis and glycolysis/gluconeogenesis, indicating their involvement in drought response. qPCR confirmed seven differentially expressed genes to be associated with drought response in M. falcata, providing valuable insights for future molecular studies and genetic breeding of legume crops.
Article
Plant Sciences
Wenbo Jiang, Yaying Xia, Xiaojia Su, Yongzhen Pang
Summary: Auxin response factor 2 (ARF2) positively regulates flavonoid biosynthesis, mainly flavonols and proanthocyanidins, in Arabidopsis. It acts in a tissue-specific manner at multiple levels and is involved in the regulation of several key regulatory and biosynthetic genes.
Article
Biotechnology & Applied Microbiology
Yaying Xia, Chunfeng He, Su Yan, Jinyue Liu, Haijun Huang, Xue Li, Qian Su, Wenbo Jiang, Yongzhen Pang
Summary: Glycine max L. accumulates isoflavonoid compounds that have beneficial effects on plant defense, plant-microbe interactions, and human health. In this study, 24 CYP82 subfamily genes were identified in soybean and their expression patterns were analyzed. One of the genes, GmCYP82D26, was found to have dual enzymatic activity and bridges the daidzein and genistein branches of the isoflavonoid pathway in soybean. This gene was preferentially expressed in roots and localized in the endoplasmic reticulum. These findings provide insights into the biosynthesis of isoflavonoids in soybean and their potential applications.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)