Article
Biochemical Research Methods
Mateusz Dembowski, John E. Rowley, Kevin Boland, Janelle Droessler, David A. Hathcoat, Alexandria Marchi, George S. Goff, Iain May
Summary: This study evaluates the application of High-Speed Counter-Current Chromatography (HSCCC) in the separation of non-radioactive rare earth elements (REEs). The results demonstrate the feasibility and effectiveness of HSCCC in separating a subset of REEs, providing potential applications in radioelement separation chemistry.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Zhibo Song, Tao Chen, Shuo Wang, Cheng Shen, Hongmei Li, Aijing Li, Peipei Li, Yumei Ma, Zhi Chen, Yulin Li
Summary: In this study, an efficient strategy for the large-scale preparation of two minor polar polyphenols from Phyllanthus emblica L. has been established using macroporous resin column chromatography and high-temperature preparative high-speed counter-current chromatography. The combination of enrichment and separation steps provides a reliable method for obtaining compounds with high purity.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Food Science & Technology
Zhiguo Jiang, Yanhua Wang, Dong Xiang, Zhengke Zhang
Summary: In this study, polysaccharides with antioxidant and hypoglycemic activities were isolated from pepper leaves, and sulfated PLP2 showed stronger antioxidant and hypoglycemic activities compared to PLP1.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Biochemical Research Methods
Shanshan Sun, Xiulan Xin, Lijun Zhu, Liang Chen, Zhaochu Xu, Yufeng Liu
Summary: This study successfully extracted three phenolic acids and two flavonoids from the fruits of Sorbus pohuashanensis using HSCCC, with purities above 95%. These compounds have beneficial effects such as anti-inflammatory, anti-tussive, anti-asthmatic and anti-cancer properties.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Analytical
Denglang Zou, Yunbin Cui, Si Li, Duocheng Sang, Weimeng Liu, Tianshu Zhao, Xueli Gu, Tao Chen, Yulin Li
Summary: High-speed counter-current chromatography is a valuable method for purifying biosynthesized products, as demonstrated in this study where glycosylation products of Silybin B were successfully separated. The optimized system allowed for simultaneous purification of multiple glycosylation products with high purity levels, showcasing the potential of this technique in preparative separation of biosynthesis products.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Analytical
Jinqian Yu, Wenxiu Chen, Lei Zhao, Tao Yue, Wencui Yang, Xiao Wang
Summary: A total of 15 compounds were successfully isolated from Polygonti rhizome using efficient techniques. Some of the compounds showed significant anti-inflammatory activities, indicating potential medicinal value.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Analytical
Hao Xu, Yuyu Niu, Yan Sun, Menglin Xia, Daozheng Lin, Wei Qiao
Summary: High-speed counter-current chromatography was successfully used to separate and purify magnoflorine, spinosin, and 6'-feruloyspinosin from Ziziphi Spinosae Semen, which showed certain antidepressant activity when tested in vitro and in vivo models.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Food Science & Technology
Xiao Li, Jing-Nan Ren, Gang Fan, Shu-Zhen Yang, Lu-Lu Zhang, Si-Yi Pan
Summary: Nootkatone, an important functional sesquiterpene, was successfully separated and purified from the fermentation broth by using high-speed counter-current chromatography (HSCCC). The selected solvent system proved to be effective in separating the target product, yielding nootkatone with high purity.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2022)
Article
Computer Science, Hardware & Architecture
Hyungjoon Bae, Yujin Hyun, Suchang Kim, Sangsoo Park, Jaeyoung Lee, Boseon Jang, Suyoung Choi, In-Cheol Park
Summary: This paper presents a high-speed counter architecture with a novel LFSR state extension. The proposed counter modifies an m-bit LFSR counter to cover 2(m) states by employing the state extension without degrading the counting rate. It consists of two sub-counters, one implemented with the proposed LFSR counter and the other with a conventional synchronous binary counter, achieving high counting rate and reducing hardware complexity.
IEEE TRANSACTIONS ON COMPUTERS
(2023)
Article
Microbiology
Weibo Sun, Bao Tang, Liangliang Dong, Jianhong Xu, Yancun Zhao, Fengquan Liu
Summary: This study successfully developed a method combining high-speed counter-current chromatography (HSCCC) with column chromatography to separate and prepare heat-stable antifungal factor (HSAF) from the crude extract of Lysobacter enzymogenes OH11. The HSCCC procedure yielded 42 mg of HSAF with a purity of 97.6% and a recovery of 91.7%. These results are helpful for industrial-scale separation and production of HSAF, and demonstrate the usefulness of HSCCC in isolating bioactive constituents from beneficial microorganisms.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Analytical
Jinqian Yu, Xiangwei Chang, Huasheng Peng, Xiao Wang, Jutao Wang, Daiyin Peng, Shuangying Gui
Summary: The study developed an efficient method for the enrichment and isolation of platycosides from Platycodi radix using MCI resin column chromatography and two modes of high-speed counter-current chromatography. The method successfully obtained various pure platycosides from the plant material, expanding the knowledge on their pharmacological activities.
ANALYTICAL METHODS
(2021)
Article
Food Science & Technology
Hongkun Xue, Pengshan Cui, Jiaqi Tan, Guowei Zhang, Shaoqin Ge, Xu Cai
Summary: In this study, high-speed counter-current chromatography (HSCCC) was successfully applied to separate dihydromyricetin (DMY) from Ampelopsis grossedentata (AG). The interaction between DMY and corn starch (CS) was investigated, and the effects on the structure and microstructure of starch-based foods were analyzed. The results showed that DMY could change the physical and microstructural properties of CS-based products, which are beneficial for industrial utilization.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Chemistry, Analytical
Yujie Li, Qiangqiang Jia, Min Zhang, Lu Kang, Zihan Li, Yu Liu, Hongyang Zhang, Ping Hu
Summary: In this study, three glucaric acids were successfully isolated and purified from Leonurus japonicus Houtt. using a combination of high-speed countercurrent chromatography and semi-preparative high-performance liquid chromatography. Cation exchange resin chromatography was applied to remove alkaloids and enrich the glucaric acid fractions. The obtained compounds were identified as 2-syringyl glucaric acid, 2,4-disyringyl glucaric acid, and 3,4-disyringyl glucaric acid, with the latter being reported for the first time.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Food Science & Technology
Chen Wang, Iftikhar Ali, Daijie Wang, Tongtong Hong, Jinjie Zhang, Chao Li, Wenge Yang
Summary: Algal polyphenols were successfully extracted and identified from marine algae, demonstrating potent antioxidant activities. The antioxidant activity of polyphenols was positively correlated with the number of benzene rings, while esterification appeared to enhance their antioxidant activity.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Xiaowei Dong, Hongping Huang, Rong Wang, Shiyu Luo, Yahui Mi, Yuqing Pan, Wei Shen, Jiamin Cui, Xiaolong Hu, Xuexiang Cheng, Xinhong Shi, Hao Wang
Summary: In this study, phenolic compounds were successfully separated from Chrysanthemum morifolium cv. Fubaiju flowers, including flavonoid glycosides and quinic acid derivatives. The isolation was achieved by using high-speed counter-current chromatography and preparative high-performance liquid chromatography. The isolated compounds were evaluated for their protective effects on H2O2-induced oxidative damage in adult retinal pigment epithelial cells.
JOURNAL OF SEPARATION SCIENCE
(2023)
