Article
Biochemistry & Molecular Biology
Aigul Kh. Valiullina, Ekaterina A. Zmievskaya, Irina A. Ganeeva, Margarita N. Zhuravleva, Ekaterina E. Garanina, Albert A. Rizvanov, Alexey V. Petukhov, Emil R. Bulatov
Summary: In recent years, adoptive cell therapy has gained new applications in the field of oncology, particularly with the development of CAR-T cells for treating malignant B-cell neoplasms. However, the use of CAR-T therapy for solid tumors remains a challenge. This study evaluated the cytotoxicity of 2nd generation CAR-T cells on various solid tumor cell lines and demonstrated a correlation between cytokine secretion and tumor cell confluence. This approach may have potential for assessing CAR-T cell efficacy and cytokine release syndrome risks in solid tumor treatment.
Article
Biotechnology & Applied Microbiology
Jijun Yu, Luoxuan Wang, Xiangya Kong, Yang Cao, Mengmeng Zhang, Zhaolin Sun, Yang Liu, Jing Wang, Beifen Shen, Xiaochen Bo, Jiannan Feng
Summary: Cancer vaccines have gained attention for their performance in preclinical and clinical settings. However, there is a need for improvement in predicting neoantigens. This study collected verified cancer antigen peptides and discussed the role of each dataset in algorithm improvement. A platform was designed for exploring cancer antigens.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Zheng-Zheng Zhang, Tian Wang, Xiao-Feng Wang, Yu-Qing Zhang, Shu-Xia Song, Cui-Qing Ma
Summary: Chimeric antigen receptor T cell (CAR-T) therapy has shown significant progress in the treatment of hematologic malignancies, with higher specificity, stronger lethality, and longer-lasting efficacy compared to traditional anti-tumor methods. However, there are still challenges in the treatment of solid tumors, including immune escape, physical barriers, immunosuppressive molecules, and T cell exhaustion. Modifying intracellular signaling of CAR, coexpressing or knocking out transcription factors, and combining CAR-T cells with other therapies are promising strategies to improve CAR-T cells function.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Hongjun Li, Zejun Wang, Edikan Archibong, Qing Wu, Guojun Chen, Quanyin Hu, Tianyuan Ci, Zhaowei Chen, Jinqiang Wang, Di Wen, Hongwei Du, Jie Jiang, Jie Sun, Xingcai Zhang, Gianpietro Dotti, Zhen Gu
Summary: A porous microneedle patch is used to load CAR T cells and escort them to solid tumors, breaking the physical barrier without losing cellular activity. This patch offers a transformative platform for scattered seeding of living cells for treating various tumors.
NATIONAL SCIENCE REVIEW
(2022)
Article
Medicine, Research & Experimental
Shuyang Sun, Ziqiang Ding, Li Gao, Bruce D. Hammock, Xianing Huang, Zhi Ping Xu, Xuan Wang, Qihong Cheng, Fengzhen Mo, Wei Shi, Shenxia Xie, Aiqun Liu, Haixia Li, Xiaomei Yang, Xiaoling Lu
Summary: The study successfully selected EGFRvIII nanobody for the construction of novel EGFRvIII Nb-CAR-T cells, demonstrating enhanced antitumor effects when combined with DC/tumor fusion vaccines. This new strategy shows promising results in boosting the efficacy of CAR-T cell therapy against solid tumors, providing a potential therapeutic platform for clinical treatment.
Article
Chemistry, Multidisciplinary
Xinyue Wang, Fanyan Meng, Xiang Li, Luxin Xue, Anni Chen, Yuling Qiu, Zhifan Zhang, Lin Li, Fengcen Liu, Yishan Li, Zhichen Sun, Yanhong Chu, Ruihan Xu, Lixia Yu, Jie Shao, Manman Tian, Xiaoping Qian, Qin Liu, Baorui Liu, Rutian Li
Summary: Chimeric antigen receptor (CAR)-T cell therapy is an effective treatment against advanced malignancies, but it can cause severe adverse events. A nanomodified switch strategy using gelatinase-responsive nanoparticles (NPs) selectively delivers a heterodimerizing switch to activate CAR-T cells only on tumors. The sustained-release effect of NPs ensures smooth activation of CAR-T cells, avoiding sudden cytokine release. This study introduces NanoSwitch as a universal and applicable solution for safety problems in CAR-T therapy.
Review
Immunology
Arthur Xuan Wang, Xiao Jing Ong, Criselle D'Souza, Paul J. Neeson, Joe Jiang Zhu
Summary: Chemotherapy is commonly used before CAR-T cell therapy to improve engraftment. It can deplete immunosuppressive cells, promote a pro-inflammatory tumor microenvironment, disrupt tumor stroma, and improve CAR-T cell recruitment. However, there are challenges and obstacles to achieving effective clinical outcomes with the combination therapy, such as dosage and treatment schedule-dependent effects.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sachiko Hirobe, Seina Nagai, Masashi Tachibana, Naoki Okada
Summary: Chimeric antigen receptor expression T (CAR-T) cell therapy has shown efficacy against relapsed/refractory B-cell malignant lymphoma and is considered an innovative cancer treatment. CAR-T cells targeting roundabout homolog 4 (Robo4), which is highly expressed in tumor vascular endothelial cells, have been constructed to overcome the challenges of solid tumor accessibility and immunosuppressive tumor microenvironment. These CAR-T cells exhibit antigen-specific cytotoxicity, cytokine production, and proliferation correlated with their binding affinity for Robo4.
Review
Biochemistry & Molecular Biology
Parvin Akbari, Afroditi Katsarou, Roxanna Daghighian, Lotte W. H. G. van Mil, Elisabeth J. M. Huijbers, Arjan W. Griffioen, Judy R. van Beijnum
Summary: In this review, recent advances in CAR T cell therapy against solid tumors, with a focus on targeting the tumor vasculature, are discussed. Opportunities to overcome challenges and barriers through engineering of CAR T cells to enhance trafficking, safety, and efficacy are also explored.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2022)
Review
Immunology
Pouya Safarzadeh Kozani, Pooria Safarzadeh Kozani, Milad Ahmadi Najafabadi, Fatemeh Yousefi, Seyed Mohamad Javad Mirarefin, Fatemeh Rahbarizadeh
Summary: CAR-T cells have revolutionized cancer treatment for hematologic malignancies, but face challenges in solid tumors due to heterogeneous antigen expression and immunosuppressive tumor microenvironments. Strategies such as boosting vaccines and novel implementations are explored to enhance CAR-T tumoricidal capacity and trigger endogenous antitumor responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Guangna Liu, Wei Rui, Xueqiang Zhao, Xin Lin
Summary: CAR-T cell therapy has shown successful outcomes in hematological malignancies but faces challenges in treating solid tumors due to immunosuppressive mechanisms in the tumor microenvironment. This review summarized inhibitory factors affecting CAR-T cell function and discussed strategies to enhance CAR-T cell efficacy in solid tumor treatment by targeting these barriers.
CELLULAR & MOLECULAR IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Vita Golubovskaya, Hua Zhou, Feng Li, Robert Berahovich, Jinying Sun, Michael Valentine, Shirley Xu, Hizkia Harto, John Sienkiewicz, Yanwei Huang, Lijun Wu
Summary: This study focused on developing novel CS1 CAR-T cells and bispecific CS1-BCMA CAR-T cells for targeting multiple myeloma. The experimental results demonstrated that these cells effectively killed multiple myeloma cells, showing promise for future clinical trials.
Article
Oncology
Xinhui Li, Jian Zhou, Weiwen Zhang, Wenhua You, Jun Wang, Linlin Zhou, Lei Liu, Wei-Wei Chen, Hanjie Li
Summary: Identification of tumor-specific surface proteins is important for the development of CAR-T cell therapies and ADCs. This study utilized the TCGA database to find tumor tissue-specific genes associated with patient survival, and identified a set of tumor-specific surface proteins that may play a role in tumor metastasis. These proteins show positive correlation and have the potential to be dual-drug targets. Our findings highlight the importance of tumor cell surface targets in the development of CAR-T and ADC-related drugs.
Article
Immunology
Cooper J. Sailer, Yeonsun Hong, Ankit Dahal, Allison T. Ryan, Sana Mir, Scott A. Gerber, Patrick M. Reagan, Minsoo Kim
Summary: Chimeric antigen receptor (CAR)-T cell therapy shows promise in treating hematologic cancers, but its success in solid tumors is limited due to CAR-T cell exhaustion and poor persistence. This study finds that PD-1(high) CAR-T cells perform better in multiple T cell functions compared to PD-1(low) CAR-T cells. However, adoptive transfer of PD-1(high) CAR-T cells alone is insufficient to control tumor growth, and a combination therapy with PD-1 blockade is needed for solid tumors.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Jin-Xuan Fan, Zhong-Bao Hu, Hui-Yun Gu, Wen-Qiang Qu, Wen Li, Bai-Wen Qi, Ai-Xi Yu, Xian-Zheng Zhang
Summary: Heterogeneity is a major obstacle in CAR-T therapy for solid tumors. This study proposes the use of lipid-modified fluorescein isothiocyanate as an artificial ligand to normalize the phenotypes of tumor cells and enhance the recognition and selectivity of CAR-T cells in solid tumors.
SCIENCE CHINA-CHEMISTRY
(2023)
Article
Endocrinology & Metabolism
Jinquan Cai, Zhixian Wu, Xiumin Xu, Lianming Liao, Jin Chen, Lianghu Huang, Weizhen Wu, Fang Luo, Chenguang Wu, Alberto Pugliese, Antonello Pileggi, Camillo Ricordi, Jianming Tan
Article
Oncology
Kai Zheng, Weizhen Wu, Shunliang Yang, Lianghu Huang, Jin Chen, Chungui Gong, Zhichao Fu, Linlin Zhang, Jianming Tan
MOLECULAR MEDICINE REPORTS
(2015)
Article
Medicine, Research & Experimental
Kai Zheng, Weizhen Wu, Shunliang Yang, Lianghu Huang, Jin Chen, Chungui Gong, Zhichao Fu, Ruofei Lin, Jianming Tan
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2016)
Article
Biochemistry & Molecular Biology
Jin Chen, Qun Ren, Yuanming Cai, Tingting Lin, Weimin Zuo, Jin Wang, Rong Lin, Ling Zhu, Ping Wang, Huiyue Dong, Hu Zhao, Lianghu Huang, Yunfeng Fu, Shunliang Yang, Jianming Tan, Xiaopeng Lan, Shuiliang Wang
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Yan Lei, Shuiliang Wang, Bingshuang Ren, Jin Wang, Jin Chen, Jun Lu, Shihuai Zhan, Yunfeng Fu, Lianghu Huang, Jianming Tan
Article
Cell Biology
Hu Zhao, Junqiu Chen, Jin Chen, Xuhui Kong, Hehuan Zhu, Yongping Zhang, Huiyue Dong, Jie Wang, Qun Ren, Qinghua Wang, Shushang Chen, Zhen Deng, Zhan Chen, Qiang Cui, Junqiong Zheng, Jun Lu, Shuiliang Wang, Jianming Tan
JOURNAL OF CELLULAR PHYSIOLOGY
(2019)
Article
Oncology
Tingting Lin, Qun Ren, Weimin Zuo, Ruxue Jia, Linhui Xie, Rong Lin, Hu Zhao, Jin Chen, Yan Lei, Ping Wang, Huiyue Dong, Lianghu Huang, Jinquan Cai, Yonghai Peng, Zongyang Yu, Jianming Tan, Shuiliang Wang
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2019)
Article
Cell & Tissue Engineering
Jin Chen, Junqiu Chen, Yuanhang Cheng, Yunfeng Fu, Hongzhou Zhao, Minying Tang, Hu Zhao, Na Lin, Xiaohua Shi, Yan Lei, Shuiliang Wang, Lianghu Huang, Weizhen Wu, Jianming Tan
STEM CELL RESEARCH & THERAPY
(2020)
Article
Cell & Tissue Engineering
Zhixian Wu, Xiumin Xu, Jinquan Cai, Jin Chen, Lianghu Huang, Weizhen Wu, Alberto Pugliese, Shasha Li, Camillo Ricordi, Jianming Tan
Summary: This study investigates the long-term safety and benefit of umbilical cord mesenchymal stromal cell (MSCs) plus autologous bone marrow mononuclear cell (aBM-MNC) stem cell transplantation (SCT) in established type 1 diabetes (T1D). The results demonstrate that this transplantation method is associated with a reduced incidence of chronic diabetes complications.
Article
Biochemistry & Molecular Biology
Ying Chen, Xiaokun Fan, Jiawei Lu, Xin Liu, Jin Chen, Yi Chen
Summary: This study presents the preparation of multifunctional chitosan/polydopamine/polyacrylamide hydrogels with superior tissue adhesion, self-healing, and antibacterial properties. The hydrogels show great potential in accelerating skin tissue regeneration and promoting wound healing.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Materials Science, Multidisciplinary
Jiawei Lu, Xiaokun Fan, Jiawei Hu, Jie Li, Jingjing Rong, Weijia Wang, Ying Chen, Wenyong Liu, Jin Chen, Yi Chen
Summary: The study reveals that a double-layer hydrogel can be used as an independent wound dressing. The first layer, C-A, has moderate adhesion and the ability to promote solution absorption, while the second layer, P-A, has good water-locking ability and high strength. The double-layer hydrogel dressing exhibits excellent comprehensive performance, promoting wound healing and possessing antibacterial effects.
MATERIALS & DESIGN
(2023)
Article
Immunology
Yachao Hou, Yanan Wang, Jin Chen, Changguo Chen
Summary: Tumor necrosis factor superfamily 14 (TNFSF14) plays different and significant roles in diverse viral infections. In COVID-19, anti-TNFSF14 monoclonal antibody treatment can reduce the risk of respiratory failure and mortality. In other viral infections, TNFSF14 can be used as a biomarker or immune adjuvant for treatment.
Article
Medicine, Research & Experimental
Jin Chen, Zhongqiu Wu, Wenyi Deng, Minying Tang, Lvying Wu, Na Lin, Liuyan Chen, Yunfeng Fu, Min Zhao, Changguo Chen, Wenting Li
Summary: USP51 regulates TWIST1 protein through deubiquitination, influencing stemness and growth of non-small cell lung carcinoma (NSCLC) cells.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Materials Science, Characterization & Testing
Ying Chen, Xiaokun Fan, Xin Liu, Cong Meng, Jingjing Rong, Xiang Lu, Jin Chen, Yi Chen
Summary: In order to address the contradiction between conductivity, tensile properties, and antibacterial properties in traditional hydrogels, a multifunctional CMCS/GG/PAM double-network (DN) hydrogel is developed by incorporating carboxymethyl chitosan (CMCS) into gellan gum/polyacrylamide (GG/PAM) DN hydrogel. CMCS not only provides excellent antibacterial properties for the hydrogel, but also enhances the interaction between the GG and PAM networks. The combination of physically crosslinked GG network and chemically crosslinked PAM network synergistically improves the mechanical performance of the hydrogel, which can be tailored by adjusting the dosage of GG and CMCS. The CMCS/GG/PAM DN hydrogel exhibits high fracture stress (198.4 kPa) and fracture strain (797.8%). It also demonstrates good adhesion to various solid surfaces, excellent biocompatibility, conductive strain sensitivity, and real-time monitoring capability for human health monitoring, making it a safe antibacterial flexible sensor.