Journal
JOURNAL OF SEPARATION SCIENCE
Volume 39, Issue 7, Pages 1278-1285Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201501358
Keywords
Crude extracts; High-speed counter-current chromatography; Phenolic compounds; Response surface methodology; Terminalia chebula Retz
Categories
Funding
- General Program of Natural Science Foundation of Qinghai Province [2015-ZJ-908]
- Science and Technology Plan of Qinghai Province [2014-GX-C01, 2014-NS-515-2]
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This study presents an efficient strategy for separation of three phenolic compounds with high molecular weight from the crude extract of Terminalia chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography. The ultrasound-assisted extraction conditions were optimized by response surface methodology and the results showed the target compounds could be well enriched under the optimized extraction conditions. Then the crude extract was directly separated by high-speed counter-current chromatography without any pretreatment using n-hexane/ethyl acetate/methanol/water (1:7:0.5:3, v/v/v/v) as the solvent system. In 180 min, 13 mg of A, 18 mg of B, and 9 mg of C were obtained from 200 mg of crude sample. Their structures were identified as Chebulagic acid (A, 954 Da), Chebulinic acid (B, 956 Da), and Ellagic acid (C) by H-1 NMR spectroscopy.
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