Article
Food Science & Technology
Yujie Zhong, Chengni Jin, Jiahui Han, Jiachang Zhu, Qi Liu, Dianjun Sun, Xiaodong Xia, Yu Zhang, Xiaoli Peng
Summary: The study shows that DIO exerts protective effects against kidney injury, mitochondrial dysfunction, and cell apoptosis by inhibiting ER stress and maintaining Ca2+ homeostasis and Bcl2 expression.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Cell Biology
Shridhar Sanghvi, Kalina Szteyn, Devasena Ponnalagu, Divya Sridharan, Alexender Lam, Inderjot Hansra, Ankur Chaudhury, Uddalak Majumdar, Andrew R. Kohut, Shubha Gururaja Rao, Mahmood Khan, Vidu Garg, Harpreet Singh
Summary: This study reveals the presence of BKCa channels in the plasma membrane and mitochondria of neonatal murine and rodent cardiomyocytes, and demonstrates their protective effect on the heart. Inhibition of plasma membrane BKCa channels can protect the neonatal heart from ischemia-reperfusion injury.
CELL DEATH DISCOVERY
(2022)
Article
Biochemistry & Molecular Biology
Ben B. Wang, Haijin Xu, Sandra Isenmann, Cheng Huang, Xabier Elorza-Vidal, Grigori Y. Rychkov, Raul Estevez, Ralf B. Schittenhelm, Gergely L. Lukacs, Pirjo M. Apaja
Summary: This study identifies Ubr1 as a protein quality control E3 ubiquitin-ligase that counteracts proteostasis stresses by facilitating endosomal cargo-selective autophagy. Ubr1 plays a critical role in endo-lysosomal proteostasis perturbations and is linked to Ca2+ homeostasis and diseases such as cancers and brain disorders.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Viviane Nascimento Da Conceicao, Yuyang Sun, Karthik Ramachandran, Arun Chauhan, Amritha Raveendran, Manigandan Venkatesan, Bony DeKumar, Soumya Maity, Neelanjan Vishnu, George A. Kotsakis, Paul F. Worley, Donald L. Gill, Bibhuti B. Mishra, Muniswamy Madesh, Brij B. Singh
Summary: The transformation of naive macrophages into M1 or M2 phenotypes is regulated by distinct Ca2+ entry channels. Blocking Ca2+ entry can suppress signaling events related to cytokine production and reduce the inflammatory response of macrophages, depending on the expression levels of Orai1 and TRPC1 channels.
Article
Medicine, Research & Experimental
Daijin Li, Jing Xu, Jie Chang, Yujue Wang, Xiaoye Du, Hanhan Wu, Jingang Cui, Peiwei Wang, Teng Zhang, Yu Chen
Summary: Through in vitro and in vivo experiments, this study demonstrates for the first time that hyperoside can protect photoreceptors from oxidative stress-induced mitochondrial dysfunction and cell death, thereby reducing the degeneration of retinal projection cells and maintaining retinal homeostasis. These findings suggest that hyperoside has the potential to be a protective agent for the treatment of related photoreceptor degenerative diseases.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Cell Biology
Youkun Bi, Yan Chang, Qun Liu, Yang Mao, Kui Zhai, Yuanli Zhou, Renjie Jiao, Guangju Ji
Summary: This study reveals a novel function of CG9911 in promoting fat storage by regulating ER Ca2+ signal in Drosophila. Mutant flies with deleted CG9911 show decreased lipid storage, increased lipolysis, and altered expression of lipolysis related genes, indicating the importance of CG9911 in lipid metabolism and energy homeostasis. Additionally, the phenotype is enhanced under ER stresses and suppressed by a reduction in ER Ca2+, suggesting the intricate regulatory mechanisms involved in fat storage in Drosophila.
Article
Cell Biology
Fanghui Chen, Le Sheng, Chenjie Xu, Jun Li, Ilyas Ali, Honglin Li, Yafei Cai
Summary: The study uncovered the crucial role of Ufbp1 in ketosis-induced liver fibrosis, where its deficiency led to Smad3 activation, increased ER stress, and enhanced hepatocyte apoptosis, resulting in liver fibrosis. Ufbp1 plays a protective role in ketosis-induced liver injury.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Medicinal
Muqing Zhang, Jiaying Qi, Qianqian He, Donglai Ma, Jing Li, Xi Chu, Saijie Zuo, Yixin Zhang, Li Li, Li Chu
Summary: Liquiritigenin (Lq) protects against myocardial ischemic injury by reducing intracellular calcium concentration through antioxidation, antiapoptosis, counteraction mitochondrial dysfunction, and inhibition of ICa-L.
PHYTOTHERAPY RESEARCH
(2022)
Article
Cell Biology
Nolwenn Tessier, Mallory Ducrozet, Maya Dia, Sally Badawi, Christophe Chouabe, Claire Crola Da Silva, Michel Ovize, Gabriel Bidaux, Fabien Van Coppenolle, Sylvie Ducreux
Summary: This study provides the first evidence that TRPV1 channels contribute to Ca2+ exchanges in MAMs.
Review
Biochemistry & Molecular Biology
Carmen Costas-Ferreira, Lilian R. F. Faro
Summary: Pesticides from different chemical classes exert toxic effects on the nervous system by altering calcium homeostasis, leading to oxidative stress, neuroinflammation, and ultimately neuronal death. Proposed strategies to counteract these effects are also discussed in the review.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Sara Awan, Magalie Lambert, Ali Imtiaz, Fabien Alpy, Catherine Tomasetto, Mustapha Oulad-Abdelghani, Christine Schaeffer, Chloe Moritz, Diane Julien-David, Souad Najib, Laurent O. Martinez, Rachel L. Matz, Xavier Collet, Roberto Silva-Rojas, Johann Bohm, Joachim Herz, Jerome Terrand, Philippe Boucher
Summary: The study reveals the crucial role of Wnt5a in maintaining cholesterol homeostasis and preventing the formation of atherosclerosis by regulating lysosomal cholesterol export, metabolic signaling, and cholesterol supply.
CIRCULATION RESEARCH
(2022)
Article
Hematology
Nan Wang, Jing Yin, Na You, Shangda Yang, Dan Guo, Yangyang Zhao, Yongxin Ru, Xiaoyan Liu, Hui Cheng, Qian Ren, Tao Cheng, Xiaotong Ma
Summary: The transcription factor TWIST1 is identified as a critical regulator of HSC maintenance by modulating mitochondrial function. Deletion of Twist1 leads to decreased HSC frequency, impaired self-renewal capacity, skewed myeloid differentiation, and compromised stress tolerance. The mechanism involves activation of voltage-gated calcium channel Cacna1b, resulting in increased mitochondrial calcium levels, metabolic activity, and reactive oxygen species production, which can be rescued by calcium channel blocker under stress conditions.
Article
Biochemistry & Molecular Biology
Tianxia Luo, Liren Li, Yanghao Peng, Rongrong Xie, Ningning Yan, Hongkun Fan, Qian Zhang
Summary: The study confirms the physical interactions between JP2 and SK2 channels, highlighting the essential role of the JP2 MORN domain in membrane expression and retrograde trafficking of SK2 channels, as well as direct modulation of SK2 channel current amplitude.
Article
Cell Biology
Perumal Arumugam Desingu, Sneha Mishra, Lavanya Dindi, Shalini Srinivasan, Raju S. Rajmani, Venkatraman Ravi, Ankit Kumar Tamta, Sukanya Raghu, Krishnega Murugasamy, Anwit Shriniwas Pandit, Nagalingam R. Sundaresan
Summary: This study demonstrates that inhibition of PARP1 can protect against Japanese encephalitis virus (JEV) infection by inhibiting autophagy.
Article
Cardiac & Cardiovascular Systems
Willeke de Haan, Wouter Dheedene, Katerina Apelt, Sofiane Decombas-Deschamps, Stefan Vinckier, Stefaan Verhulst, Andrea Conidi, Thomas Deffieux, Michael W. Staring, Petra Vandervoort, Ellen Caluwe, Marleen Lox, Inge Mannaerts, Tsuyoshi Takagi, Joris Jaekers, Geert Berx, Jody Haigh, Baki Topal, An Zwijsen, Yujiro Higashi, Leo A. van Grunsven, Wilfred F. J. van IJcken, Eskeatnaf Mulugeta, Mickael Tanter, Franck P. G. Lebrin, Danny Huylebroeck, Aernout Luttun
Summary: This study demonstrated the specific role of Zeb2 in liver endothelial cells, showing that Zeb2 deficiency leads to significant changes in gene expression related to angiogenesis and potential promotion of endothelial dedifferentiation. Furthermore, Zeb2 regulates LSEC-HSC communication and HSC activation to maintain vascular structure and inhibit liver fibrosis.
CARDIOVASCULAR RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Juliane Rizzi, Tatiana Rojo Moro, Sheila Maria Brochado Winnischofer, Guilherme Augusto Colusse, Camila Silva Tamiello, Marina Trombetta-Lima, Guilhermina Rodrigues Noleto, Amalia M. Dolga, Maria Eugenia Rabello Duarte, Miguel Daniel Noseda
Summary: Several polysaccharides can enhance immune responses and can be derived from microalgae. In this study, a water-soluble polysaccharide from Conticribra weissflogii was identified as a (1 -> 3)-linked beta-D-glucan with a low proportion of C-6 substitution. The beta-D-glucan showed immunomodulatory properties by enhancing the phagocytic activity of macrophages.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Asmaa Oun, Emmy Hoeksema, Ahmed Soliman, Famke Brouwer, Fabiola Garcia-Reyes, Henderikus Pots, Marina Trombetta-Lima, Arjan Kortholt, Amalia M. Dolga
Summary: Dysfunction of the immune system and mitochondrial metabolism is associated with Parkinson's disease (PD) pathology. Mutations and increased kinase activity of leucine-rich repeat kinase 2 (LRRK2) are linked to both idiopathic and familial PD. However, the function of LRRK2 in immune cells under inflammatory conditions is contradictory.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Raphael de Avila Narciso Gomes, Alejandro Marmolejo-Garza, Floris-Jan Haan, Teresa Mitchell Garcia, Tingting Chen, Mario Mauthe, Yollanda E. Moreira Franco Parisotto, Mario Minor Murakami Jr, Suely Kazue Nagahashi Marie, Mauricio S. Baptista, Amalia M. Dolga, Marina Trombetta-Lima
Summary: Photodynamic therapy (PDT) is a promising approach for treating central nervous system (CNS) disorders. This study investigated the photodamage mechanisms of the photosensitizer 1,9-dimethyl-methylene blue (DMMB) in neuronal cells. The results indicated that DMMB-PDT induced cell death and increased reactive oxygen species (ROS) production. It also caused mitochondrial dysfunction and autophagic dysfunction, leading to cytotoxicity in neuronal cells.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Correction
Cell Biology
Wei Han, Eva-Maria Meissner, Stefanie Neunteibl, Madeline Guenther, Joerg Kahnt, Amalia Dolga, Cuicui Xie, Nikolaus Plesnila, Changlian Zhu, Klas Blomgren, Carsten Culmsee
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Wei Han, Eva-Maria Meissner, Stefanie Neunteibl, Madeline Gunther, Joerg Kahnt, Amalia Dolga, Cuicui Xie, Nikolaus Plesnila, Changlian Zhu, Klas Blomgren, Carsten Culmsee
Summary: Neural stem and progenitor cell (NSPC) transplants provide neuroprotection by releasing a protective secretome, containing heat-stable proteins, that promote neuronal survival in acute brain injury models.
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Gwenda F. Vasse, Sara Russo, Andrei Barcaru, Asmaa A. A. Oun, Amalia M. Dolga, Patrick van Rijn, Marcel Kwiatkowski, Natalia Govorukhina, Rainer Bischoff, Barbro N. Melgert
Summary: Idiopathic pulmonary fibrosis is a progressive lung disease that causes scarring and loss of lung function. Macrophages respond to altered morphological and mechanical properties of the extracellular matrix in fibrosis and can change their expression and activity of metabolism-related proteins. These findings suggest important interactions between macrophages and their surroundings in the repair or fibrosis of lung tissue.
SCIENTIFIC REPORTS
(2023)
Review
Physiology
Tong Zhang, Minh D. A. Luu, Amalia M. M. Dolga, Ulrich L. M. Eisel, Martina Schmidt
Summary: Alzheimer's disease (AD) and Parkinson's disease (PD) are the most prevalent neurodegenerative disorders worldwide, impacting millions of people's life expectancy and quality. Recent research suggests overlapping mechanisms may underlie AD and PD, with novel cell death mechanisms, such as parthanatos, netosis, lysosome-dependent cell death, senescence, and ferroptosis, being modulated by cAMP signaling via PKA and Epac. This review focuses on the overlapping mechanisms between AD and PD, specifically in relation to cAMP signaling and the pharmacology of cAMP signaling pathways.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tingting Chen, Nad'a Majernikova, Alejandro Marmolejo-Garza, Marina Trombetta-Lima, Angelica Maria Sabogal-Guaqueta, Yuequ Zhang, Ruth ten Kate, Minte Zuidema, Patty P. M. F. A. Mulder, Wilfred den Dunnen, Reinoud Gosens, Elisabeth Verpoorte, Carsten Culmsee, Ulrich L. M. Eisel, Amalia M. Dolga
Summary: Transplanting exogenous mitochondria into neuronal cells can protect cells from ferroptotic cell death by reducing lipid peroxidation and mitochondrial superoxide production. The function of mitochondrial complexes is crucial for the neuroprotective effects of exogenous mitochondria.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Jessica Hernandez, Julia Schaeffer, Christiane Herden, Fabian Johannes Pflieger, Sylvia Reiche, Svenja Koerber, Hiromu Kitagawa, Joelle Welter, Susanne Michels, Carsten Culmsee, Jens Bier, Natascha Sommer, Jing X. Kang, Konstantin Mayer, Matthias Hecker, Christoph Rummel
Summary: Specialized pro-resolving mediators (SPMs), particularly Resolvin E1 (RvE1), can effectively resolve inflammation and promote healing in lung diseases like acute respiratory distress syndrome (ARDS). By studying the interaction between the lung and brain during ARDS and the therapeutic potential of SPMs and its derivatives, researchers induced ARDS in fat-1 mice crossed with RvE1 receptor knockout mice. The study found that LPS-induced lung inflammation increased inflammatory signaling in the hypothalamus, indicating immune-to-brain communication during ARDS. Deficiency in RvE1 receptors and altered profiles of inflammatory and lipid mediators affected the lung-brain interaction during ARDS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Shashipavan Chillappagari, Andreas Guenther, Poornima Mahavadi
Summary: Idiopathic pulmonary fibrosis (IPF) is a deadly disease with unknown causes and no cure. Autophagy, a cellular surveillance pathway, is insufficiently activated in both alveolar epithelial type II cells and fibroblasts of IPF patient lungs. Modulating this pathway through the co-chaperone Bcl2-associated athanogene3 (BAG3) may provide a promising novel therapeutic approach for IPF, by facilitating the degradation of accumulated substances and influencing cell fate.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Melanie Merkel, Bjarne Goebel, Moritz Boll, Aasha Adhikari, Viktoria Maurer, Dieter Steinhilber, Carsten Culmsee
Summary: Ferroptosis is a type of oxidative cell death characterized by lipid peroxidation and mitochondrial impairment. The enzymes ACSL4 and LPCAT2 are crucial for the biosynthesis of PUFA-containing phospholipids, providing substrates for lipid peroxidation and promoting ferroptosis. Overexpression of ACSL4 and LPCAT2 in HEK293T cells increased sensitivity to RSL3-induced cell death and resulted in mitochondrial dysfunction. Mitochondrial ROS production and organelle disintegration are essential for mediating ferroptosis initiated by lipid peroxidation.
Article
Biochemistry & Molecular Biology
Tong Zhang, Nshunge Musheshe, Christina H. J. T. M. van der Veen, Helmut W. Kessels, Amalia Dolga, Peter De Deyn, Ulrich Eisel, Martina Schmidt
Summary: Alzheimer's disease (AD) is a neurodegenerative disease characterized by the accumulation of amyloid beta (A beta) and hyperphosphorylated tau in the brain. Recent studies have shown that memory retrieval is impaired in the early stage of AD. This study aimed to investigate the expression of proteins associated with Epac2-mediated memory in hippocampal postmortem samples of AD patients and experimental AD model mice.
Article
Chemistry, Physical
Beatrice Corci, Oscar Hooiveld, Amalia M. Dolga, Christoffer Aberg
Summary: The motion of molecules, organelles, and foreign objects within living cells has been studied in various organisms. This study focuses on the motion of mitochondria in mammalian cells and observes that mitochondria remain in a limited region for a long time before moving longer distances quickly. The motion of mitochondria shows glass-like characteristics, and this behavior is also observed in other objects and different cell types.
Article
Multidisciplinary Sciences
Natalia Orti-Casan, Ate S. S. Boerema, Karina Kopke, Amber Ebskamp, Jan Keijser, Yuequ Zhang, Tingting Chen, Amalia M. M. Dolga, Kerensa Broersen, Roman Fischer, Klaus Pfizenmaier, Roland E. E. Kontermann, Ulrich L. M. Eisel
Summary: Tumor necrosis factor alpha (TNF-α) has been recognized as a therapeutic target for inflammatory and neurodegenerative diseases. In this study, the TNFR1-specific antagonist Atrosimab was found to attenuate cognitive impairments, reduce neuroinflammation, and neuronal cell death in an acute neurodegenerative mouse model. These results suggest that Atrosimab may be a promising candidate for the treatment of neurodegenerative diseases.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Yang Luo, Laurent Chatre, Shaden Melhem, Zayana M. Al-Dahmani, Natalie Z. M. Homer, Anneke Miedema, Leo E. Deelman, Matthew R. Groves, Martin Feelisch, Nicholas M. Morton, Amalia Dolga, Harry van Goor
Summary: This study reveals that TST deficiency leads to disturbances in the reactive species interactome in the brain cortex, resulting in altered ROS and RSS (specifically, polysulfide) generation, as well as mitochondrial OXPHOS remodeling. These changes are associated with abnormal Nrf2-Keap1 expression and thiol-dependent antioxidant function. Tst-/- mice exhibit lower antioxidant capacity compared to wildtype controls when challenged with the redox-active herbicide paraquat.