Article
Environmental Sciences
Nadine Belkouteb, Henning Schroeder, Julia Arndt, Jan G. Wiederhold, Thomas A. Ternes, Lars Duester
Summary: Triple-quadrupole inductively coupled plasma mass spectrometry (ICP-QQQ-MS) is a unique analytical technique used for determining total element concentrations in various matrices, with wide linear range and short analysis times. By applying this method in future river water monitoring networks, a better understanding of natural transport processes and global biogeochemical element cycles can be achieved, and the number of methods can be reduced.
Article
Environmental Sciences
Jingrong He, Li Li, Yunxiang Li, Mei Huang, Ying Zhu, Shihuai Deng
Summary: In this study, six aromatic organosulfates were synthesized and their purity and concentration were determined using high performance liquid chromatography/ultraviolet detector and high performance liquid chromatography/electrospray ionization/tandem mass spectrometry. The characteristic ion fragments were found to improve the identification and detection of target compounds.
ATMOSPHERIC ENVIRONMENT
(2022)
Article
Chemistry, Analytical
Erick G. Baez Bolivar, Duong T. Bui, Elena N. Kitova, Ling Han, Ruixiang B. Zheng, Erik J. Luber, Sayed Youssef Sayed, Lara K. Mahal, John S. Klassen
Summary: This study demonstrates the facile quantification of low-affinity glycan-GBP interactions using nanoflow ESI emitters and electrospray ionization mass spectrometry (ESI-MS). The use of submicron emitters effectively eliminates nonspecific binding, enabling direct measurement of interactions with affinities as low as 5 mM. Additionally, this technique allows for the measurement of glycan affinities on specific GBPs, such as C-type lectins, and the influence of calcium ions on these interactions.
ANALYTICAL CHEMISTRY
(2021)
Article
Agronomy
Federica Carucci, Gustavo Moreno-Martin, Yolanda Madrid-Albarran, Giuseppe Gatta, Pasquale De Vita, Marcella Michela Giuliani
Summary: The study evaluated the effects of foliar application of selenate and organic nitrogen and sulfur sources on the selenium content and species in durum wheat grain. The results showed that foliar application of selenate led to increased selenium accumulation, particularly under conditions of lower rainfall. Organic foliar sulfur had a negative effect on selenium content, while organic foliar nitrogen increased it. Modern varieties generally had higher selenium content compared to old varieties.
Article
Biochemical Research Methods
Stasia Harycki, Alexander Gundlach-Graham
Summary: Single-particle inductively coupled plasma time-of-flight mass spectrometry (spICP-TOFMS) is a high-throughput method for analyzing nanoparticles (NPs). However, matrix interferences pose challenges for accurate NP sizing and number concentration determination. Online microdroplet calibration is a method that uses multi-element droplet standards mixed with nebulized aerosols from a NP-containing sample to calibrate the measurement. This method can correct for the effects of organic solvents on NP measurements.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Tamara Fernandez-Bautista, Beatriz Gomez-Gomez, Roberto Palacin-Garcia, Emma Gracia-Lor, Teresa Perez-Corona, Yolanda Madrid
Summary: The distribution of Se and Hg in different protein fractions extracted from muscle tissues of tuna, swordfish, and salmon (wild vs. farmed) was investigated for the first time. The study revealed that Se and Hg were associated with proteins of specific molecular weights, with differences observed between farmed and wild salmon. Additionally, Se speciation studies showed SeMeSeCys as the primary selenium species found in the proteins analyzed.
Article
Chemistry, Analytical
Inga Sowik, Joanna Zajda, Lena Ruzik
Summary: The market for vegetarian and vegan products has been rapidly growing in recent years, driven by consumers who are choosing to limit their meat intake. Among vegan products, plant-based drinks, burgers, yoghurts, and cheese are the most popular choices.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Qi Kang, Beibei Chen, Man He, Bin Hu
Summary: A simple and direct ICP-MS-based assay was developed for the simultaneous detection of three miRNAs by combining the lanthanide labeling strategy with entropy-driven catalytic amplification. This method allows for flexible probe design, good sensitivity, and excellent specificity.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Jiaxuan Zhang, Yang Zhou, Dongliang Zuo, Limin Yang, Xiaowen Yan, Pingguo Liu, Qiuquan Wang
Summary: A new method has been developed for quantifying active selenols in cells through specific recognition and signal switching, with a low limit of detection. Experimental results indicate significant differences in the levels of active selenols between liver cancer cells and normal cells, with a positive correlation to the degree of hepatic disease.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Marie Bagge Jensen, Petra Loznjak Svarc, Jette Jakobsen
Summary: Growing evidence of the importance of vitamin K in human health beyond blood coagulation and bone health necessitates further research. A method for extraction, lipase treatment, clean-up, and detection and quantification of various forms of vitamin K was developed and validated in a single laboratory. Deuterium labelled MK-7 can be used as an internal standard for MK-9, showing the potential for accurate quantification.
Article
Biochemistry & Molecular Biology
Ryo Konno, Takashi Matsui, Hiroaki Ito, Yusuke Kawashima, Makoto Itakura, Yoshio Kodera
Summary: GeLC-MS/MS method coupled with stable isotope dimethyl labeling provides high accuracy and comprehensive peptide comparisons, enabling accurate determination of peptide abundance ratio between samples, unaffected by the reproducibility of gel extraction or LC-MS procedures, thus facilitating the detection of proteolysis events.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Mojgan Sadat Shushtarian, Mohammad Hadi Givianrad, Mohammad Saber Tehrani, Parviz Aberoomand-Azar
Summary: This study developed an efficient strategy utilizing ion imprinted polymer (IIP) based mu-TLC-LA-ICP-MS for speciation analysis of inorganic arsenic. The IIP was characterized using FTIR and FESEM, and a TLC plate was successfully prepared. Under optimized conditions, the method demonstrated a low detection limit and wide linear dynamic range, with good performance in wastewater and human blood plasma samples.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Lana Abou-Zeid, Albert Pell, Marta Garcia Cortes, Helene Isnard, Pascale Delangle, Carole Bresson
Summary: This study developed an analytical method to determine the affinity of biomimetic peptides for uranyl ions and evaluate the effect of structural parameters on this affinity. By combining hydrophilic interaction chromatography (HILIC) with electrospray ionization mass spectrometry (ESI-MS) and inductively coupled plasma mass spectrometry (ICP-MS), the UO22+ complexes formed with different peptides were successfully separated and quantified. The results showed that an increasing number of phosphorylated residues promotes the affinity of the peptides for UO22+, while the position of the phosphorylated residues in the peptide backbone has minimal impact on this affinity, and the cyclic structure of the peptide favors the complexation with UO22+ compared to the linear structure.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Yan-Qing Chen, Hong Shen, Ru-Jie Yang, Jian-Bo Wan
Summary: A sensitive method was developed to accurately determine 20 NAEs in biological samples by chemical isotope labeling strategy coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Derivatized NAEs provided stable and abundant specific product ions in MS/MS spectrum for quantitation, enhancing detection sensitivity by 6- to 170-fold compared to non-derivatized NAEs. The method was successfully applied to determine NAEs in serum samples and liver tissues, showing practicability in analyzing endogenous NAE in biological samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Food Science & Technology
Edilza Silva do Nascimento, Katya Anaya, Julia Mariano Caju de Oliveira, Jose Thalles Jocelino Gomes de Lacerda, Michael Edward Miller, Meriellen Dias, Maria Anita Mendes, Juliana de Azevedo Lima Pallone, Clarice Weis Arns, Maria Aparecida Juliano, Tatiane Santi Gadelha, Maria Teresa Bertoldo Pacheco, Carlos Alberto de Almeida Gadelha
Summary: This study identified bioactive peptides generated by in vitro gastrointestinal digestion of yam proteins. The peptides showed antioxidant, DNA damage protection, ACE-inhibitory, and antibacterial activities. The total gastrointestinal phase hydrolysate displayed stronger DNA damage protection and antibacterial activity against Escherichia coli.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Physics, Atomic, Molecular & Chemical
Stefanie Ickert, Johanna Hofmann, Jens Riedel, Sebastian Beck, Kevin Pagel, Michael W. Linscheid
EUROPEAN JOURNAL OF MASS SPECTROMETRY
(2018)
Article
Chemistry, Applied
Yasmin M. Shaalan, H. Handoussa, R. A. Youness, R. A. Assal, A. H. El-Khatib, M. W. Linscheid, H. M. El Tayebi, A. I. Abdelaziz
NATURAL PRODUCT RESEARCH
(2018)
Review
Spectroscopy
Michael W. Linscheid
MASS SPECTROMETRY REVIEWS
(2019)
Article
Biochemistry & Molecular Biology
Ahmed Sekkien, Noha Swilam, Sherif S. Ebada, Ahmed Esmat, Ahmed H. El-Khatib, MichaelW. Linscheid, Abdel Nasser Singab
Article
Biochemical Research Methods
Stefanie Ickert, Jens Riedel, Sebastian Beck, Michael W. Linscheid
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2018)
Article
Biochemistry & Molecular Biology
Estefania Moreno-Gordaliza, Diego Esteban-Fernandez, Alberto Lazaro, Sarah Aboulmagd, Blanca Humanes, Alberto Tejedor, Michael W. Linscheid, M. Milagros Gomez-Gomez
JOURNAL OF LIPID RESEARCH
(2018)
Article
Food Science & Technology
Khaled A. Nematallah, Nahla A. Ayoub, Essam Abdelsattar, Meselhy R. Meselhy, Mohey M. Elmazar, Ahmed H. El-Khatib, Michael W. Linscheid, Rania M. Hathout, Kavitha Godugu, Aya Adel, Shaker A. Mousa
JOURNAL OF FUNCTIONAL FOODS
(2018)
Article
Biochemical Research Methods
Sabrina Trog, Ahmed H. El-Khatib, Sebastian Beck, Marcus R. Makowski, Norbert Jakubowski, Michael W. Linscheid
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Medicinal
Hesham El-Askary, Heba Handoussa, Farid Badria, Ahmed H. El-Khatib, Abdulrhman Alsayari, Michael W. Linscheid, Amira Abdel Motaal
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY
(2019)
Article
Chemistry, Analytical
David Benda, Sebastian Beck, Michael W. Linscheid
Article
Biochemical Research Methods
Stefanie Ickert, Timm Schwaar, Andreas Springer, Marko Grabarics, Jens Riedel, Sebastian Beck, Kevin Pagel, Michael W. Linscheid
JOURNAL OF MASS SPECTROMETRY
(2019)
Article
Food Science & Technology
Reham Wagdy, Reham M. Abdelkader, Ahmed H. El-Khatib, Michael W. Linscheid, Nabila Hamdi, Heba Handoussa
JOURNAL OF FOOD SCIENCE
(2019)
Article
Biochemical Research Methods
Pablo Lores Lareo, Michael W. Linscheid, Oliver Seitz
JOURNAL OF MASS SPECTROMETRY
(2019)
Article
Chemistry, Medicinal
A. N. Hashim, N. F. Swilam, E. S. Moustafa, S. M. Bakry, R. M. Labib, H. H. Barakat, A. B. Singab, M. W. Linscheid, M. A. Nawwar
Article
Biochemical Research Methods
Lena Ruhe, Stefanie Ickert, Sebastian Beck, Michael W. Linscheid
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2018)
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)