Review
Biochemistry & Molecular Biology
Georgia K. Atkin-Smith
Summary: The clearance of dying cells is a well-defined process involving molecular factors and different mechanisms for different types of cell death. It is essential for preventing excessive release of pro-inflammatory mediators and inflammatory diseases.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2021)
Article
Chemistry, Analytical
Emma B. Hartnett, Mengli Zhou, Yi-Nan Gong, Yu-Chih Chen
Summary: The study developed a label-free method to classify cell death status using machine learning and image processing. It enables the precise categorization of live, apoptotic, and necrotic cells and allows for tracking the time dynamics of cell death process, enhancing the understanding of cell death regulation at the molecular level.
ANALYTICAL CHEMISTRY
(2022)
Article
Oncology
Lucas Eduardo Botelho de Souza, Fernanda Ursoli Ferreira, Carolina Hassibe Thome, Heloisa Brand, Maristela Delgado Orellana, Vitor Marcel Faca, Aparecida Maria Fontes, Dimas Tadeu Covas
Summary: Melanoma cells have the capability to activate an EMT-like program and acquire metastatic traits through distinct pathways induced by MSCs' secretome, both in mouse and human cells.
Article
Biochemistry & Molecular Biology
Ananda Baral, Pil-Hoon Park
Summary: Leptin induces cytotoxicity in rat hepatocytes by activating NLRP3 inflammasomes, mediated through ROS production, ER stress, and autophagy. Inhibition of inflammasomes protects hepatocytes from leptin-induced cell death.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Mart M. Lamers, Anna Z. Mykytyn, Tim Breugem, Yiquan Wang, Douglas C. Wu, Samra Riesebosch, Petra B. van den Doel, Debby Schipper, Theo Bestebroer, Nicholas C. Wu, Bart L. Haagmans
Summary: Virus propagation in different cell conditions may lead to adaptive mutations, but propagating SARS-CoV-2 in human airway cells expressing serine proteases can prevent these adaptations.
Article
Engineering, Biomedical
Yizuo Song, Xin Shou, Bo Sheng, Jie Mei, Keqing Shi, Luoran Shang, Xueqiong Zhu
Summary: A multichannel microfluidic chip is developed to screen different sourced mesenchymal stem cells (MSCs) with the greatest tropism toward cervical cancer cells. Cell membranes from chorionic plate-derived MSCs are isolated to prepare membrane-camouflaged platinum prodrug composite nanoparticles for cervical cancer treatment. The composite nanoparticles effectively target tumor sites and have therapeutic effects both in vitro and in vivo, demonstrating the great value of cell membrane-camouflaged nanomedicine for targeted cancer therapy.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Cell Biology
Yoshitaka Furuta, Zheng Zhou
Summary: Phosphatidylserine (PS) is asymmetrically distributed in the plasma membrane, with inner leaflet placement in live cells and exposure on outer surfaces in apoptotic cells. This exposed PS acts as an "eat-me signal" to attract neighboring engulfing cells. This article will review the mechanisms regulating PS exposure in necrotic and apoptotic cells, highlighting their similarities and differences.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Environmental Sciences
Chin-Yin Lin, Yih-Shou Hsieh, Shu-Chen Chu, Li-Sung Hsu, Shih-Chien Huang, Pei-Ni Chen
Summary: This study found that ethanoic Cinnamomum cassia extracts (CCE) have inhibitory effects on the invasion, migration, and cancer stemness of osteosarcoma cells. CCE can reverse epithelial-to-mesenchymal transition and suppress the expression of stemness genes, leading to reduced proliferation and induced apoptosis in the cancer cells.
ENVIRONMENTAL TOXICOLOGY
(2022)
Review
Cell Biology
Yanina Bogliotti, Mark Vander Roest, Aras N. Mattis, Robert G. Gish, Gary Peltz, Robin Anwyl, Salah Kivlighn, Eric R. Schuur
Summary: Liver disease is a major cause of global mortality, with late-stage liver disease being characterized by hepatocyte injury, inflammation, and aberrant tissue healing. Treatment options for liver disease are limited, but cell replacement therapy using hepatocyte-like cells shows promise as a safe and effective treatment option.
Review
Cell & Tissue Engineering
Yizuo Song, Hejing Liu, Shuya Pan, Xinli Xiang, Miaomiao Ye, Xueqiong Zhu
Summary: Mesenchymal stromal cells (MSCs) have shown great promise in various fields such as regenerative medicine and cancer therapy. However, their role as important components within the tumor microenvironment poses potential tumorigenic risks, hindering their clinical translation.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Cell Biology
Dixin Cui, Sihan Yu, Xin Zhou, Ying Liu, Lu Gan, Yue Pan, Liwei Zheng, Mian Wan
Summary: Traditional apexification treatment for immature permanent teeth with pulp necrosis may lead to abnormal root morphology and compromised long-term prognosis. Regeneration endodontics procedures (REPs), including both cell-free and cell-based approaches, have shown promising potential in periapical lesion healing and continued root development. However, challenges remain in achieving full regeneration of the pulp-dentin complex.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Anatomy & Morphology
Roya Khamooshi, Ali Salimi, Raheleh Halabian, Pardis Saeedi
Summary: This study found that LPS-CM can significantly increase the apoptosis rate of colorectal cancer cells, decrease the expression level of the bcl2 gene, and significantly increase the expression levels of the bax and p53 genes. Therefore, LPS-CM could be a potential strategy for inhibiting the proliferation and progression of colorectal cancer cells.
Article
Cell & Tissue Engineering
Ping Wang, Yan Cui, Jing Wang, Donghua Liu, Yue Tian, Kai Liu, Xue Wang, Lin Liu, Yu He, Yufeng Pei, Li Li, Liying Sun, Zhijun Zhu, Dehua Chang, Jidong Jia, Hong You
Summary: This study compared the therapeutic effects of mesenchymal stem cells (MSCs) and N-acetylcysteine (NAC) on acetaminophen (APAP)-induced liver failure and explored the underlying mechanism. The results showed that MSCs had comparable therapeutic effects to NAC, but had slightly different effects on hepatocyte necrosis and cell infiltration, and attenuated hepatocyte necrosis by secreting hepatocyte growth factor (HGF). These findings provide a theoretical basis for the clinical application of MSCs in treating APAP liver failure.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Sun A. Ock, Seo-Yeon Kim, Won Seok Ju, Young-Im Kim, Ha-Yeon Wi, Poongyeon Lee
Summary: In this study, hepatocyte organoids (HOs) were generated using frozen-thawed primary hepatocytes (PHs) in a three-dimensional (3D) culture system. By co-culturing with adipose tissue-derived mesenchymal stem cells (A-MSCs), the HOs showed improved liver function and increased in vitro lifespan compared to conventional two-dimensional (2D) culture. This improvement makes the HOs suitable for large-scale human hepatotoxicity testing and precise livestock nutrition assessment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Arezou Khosrojerdi, Sara Soudi, Ahmad Zavaran Hosseini, Sahar Ghaffari Khaligh, Seyed Mahmoud Hashemi
Summary: This study investigated the effect of different treatments on systemic and local inflammation in a mouse model of sepsis. MSC-derived exosomes combined with imipenem controlled inflammation and increased TCD4+ and Treg cell populations. Hepatocyte-derived exosomes combined with imipenem reduced liver inflammation and increased TCD8+ and Treg cell populations. The mixture of exosomes combined with imipenem showed the best function in reducing inflammation, maintaining all T lymphocyte populations, reducing liver damage, and increasing survival rate.
Article
Biophysics
Wolfgang A. Bethge, Matthias Eyrich, Stephan Mielke, Roland Meisel, Dietger Niederwieser, Paul G. Schlegel, Ansgar Schulz, Johann Greil, Donald Bunjes, Arne Brecht, Jurgen Kuball, Michael Schumm, Vladan Vucinic, Markus Wiesneth, Halvard Bonig, Kasper Westinga, Stefanie Biedermann, Silke Holtkamp, Sandra Karitzky, Michaela Malchow, Christiane Siewert, Rupert Handgretinger, Peter Lang
Summary: Haploidentical stem cell transplantation using TCR alpha beta/CD19-depleted grafts has shown promising results in high-risk patients, with low incidences of acute GVHD and relatively high overall and disease-free survival rates at 2 years post-transplantation.
BONE MARROW TRANSPLANTATION
(2022)
Article
Virology
Ortwin Adams, Marcel Andree, Denise Rabl, Philipp N. Ostermann, Heiner Schaal, Erik Lehnert, Stefanie Ackerstaff, Lisa Mueller, Johannes C. Fischer
Summary: The study found that COVID-19 patients had relatively higher antibody levels against four seasonal human coronaviruses, with only slight elevation in levels for HCoV-HKU1 and HCoV-229E; Antibody levels against SARS-CoV-2 were significantly correlated with disease severity, while no association was found for antibodies against seasonal human coronaviruses.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Immunology
Maryam Hejazi, Congcong Zhang, Sabrina B. Bennstein, Vera Balz, Sarah B. Reusing, Melissa Quadflieg, Keven Hoerster, Stefan Heinrichs, Helmut Hanenberg, Sebastian Oberbeck, Marcus Nitsche, Sophie Cramer, Rita Pfeifer, Pranav Oberoi, Heiko Ruehl, Johannes Oldenburg, Peter Brossart, Peter A. Horn, Florian Babor, Winfried S. Wels, Johannes C. Fischer, Nina Moeker, Markus Uhrberg
Summary: This study reveals a novel functional divergence between NK cell subsets upon in vitro stimulation, marked by CD33 expression. By choosing suitable stimulation protocols, it is possible to preferentially generate CD33(+) NK cells combining efficient target cell killing and cytokine production, or alternatively CD33(-) NK cells, which produce less cytokines but are more efficient in antibody-dependent applications.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nina Hater, Katharina M. Iwaniuk, Carina Leifeld, Pia Grueten, Constanze Wiek, Katharina Raba, Fan Zhang, Johannes C. Fischer, Paul R. Andreassen, Helmut Hanenberg, Hans-Ingo Trompeter
Summary: This study investigates the expression profiles of microRNAs in different DNA repair-deficient cells and validates the regulatory effects of miR-141-3p, miR-369-3p, miR-15a-5p, miR-494-3p, and miR-544a on RAD51B and RAD51D. Further analysis reveals the widespread regulatory influence of these microRNAs on other FA/HR factors, and miR-494-3p directly reduces RAD51 foci formation while the mixture of miR-15a-5p, miR-494-3p, and miR-544a reduces HR activity.
HUMAN MOLECULAR GENETICS
(2022)
Editorial Material
Hematology
Jannik Rothenburg, Silke Rink-Baron, Lisa Mueller, Philipp Niklas Ostermann, Johannes Fischer, Johannes Stegbauer, Anja Moldenhauer
Summary: Due to the unexpected outcomes in using convalescent plasma and its varying and often low concentrations of COVID-19 antibodies, German guidelines do not recommend its use. Instead, a strategy involving the selective collection and concentration of human immunoglobulins using immunoadsorption has been adopted.
TRANSFUSIONSMEDIZIN
(2022)
Article
Multidisciplinary Sciences
Noemi F. Freise, Smaranda Gliga, Johannes Fischer, Nadine Lubke, Matthias Lutterbeck, Miriam Scholer, Edwin Bolke, Hans Martin Orth, Torsten Feldt, Christoph Roemmele, Dominik Wilke, Jochen Schneider, Kai Wille, Christian Hohmann, Richard Strauss, Martin Hower, Andreas Ruf, Joerg Schubert, Nora Isberner, Melanie Stecher, Lisa Pilgram, Jorg J. Vehreschild, Frank Hanses, Tom Luedde, Bjorn Jensen
Summary: Establishing the optimal treatment for COVID-19 patients, especially immunocompromised and pre-diseased patients, remains challenging. Reliable data on the therapeutic use of convalescent plasma (CP) for high-risk patients are lacking. This study found that early administration of CP can reduce mortality in COVID-19 patients.
SCIENTIFIC REPORTS
(2022)
Article
Biology
Claudia Lehmann, Henry Loeffler-Wirth, Vera Balz, Juergen Enczmann, Ramona Landgraf, Nicole Lakowa, Thomas Gruenewald, Johannes C. C. Fischer, Ilias Doxiadis
Summary: Since the beginning of the SARS-CoV-2 pandemic, immunogenetics has been found to play a crucial role in the susceptibility and severity of infection. This study investigated the association between various HLA alleles, blood group systems, and the molecular detection of COVID-19. The results showed that certain HLA alleles and blood group antigens were enriched in either the positive or negative group, suggesting their potential influence on COVID-19 infection.
Letter
Oncology
Roland Meisel
JOURNAL OF CLINICAL ONCOLOGY
(2023)
Article
Oncology
Tim Flaadt, Ruth L. Ladenstein, Martin Ebinger, Holger N. Lode, Helga Bjoerk Arnardottir, Ulrike Poetschger, Wolfgang Schwinger, Roland Meisel, Friedhelm R. Schuster, Michaela Doering, Peter F. Ambros, Manon Queudeville, Joerg Fuchs, Steven W. Warmann, Juergen Schaefer, Christian Seitz, Patrick Schlegel, Ines B. Brecht, Ursula Holzer, Tobias Feuchtinger, Thorsten Simon, Johannes H. Schulte, Angelika Eggert, Heiko-Manuel Teltschik, Toni Illhardt, Rupert Handgretinger, Peter Lang
Summary: This study investigates the use of haploidentical stem cell transplantation and the anti-GD2 antibody dinutuximab beta (DB) in treating patients with relapsed high-risk neuroblastoma (rHR-NB). The results show that DB therapy is feasible and improves the chances of cure in rHR-NB patients.
JOURNAL OF CLINICAL ONCOLOGY
(2023)
Article
Medical Laboratory Technology
Anja Moldenhauer, Peter Hellstern, Till Hoffmann, Johannes C. Fischer
Summary: This study found significant differences in peak blood concentrations of rivaroxaban based on age and gender. Women and patients over 60 years old had higher peak concentrations. The findings suggest that gender and age should be taken into account when determining reference values for blood concentrations.
CLINICAL LABORATORY
(2023)
Article
Biochemistry & Molecular Biology
Nikolas H. Stoecklein, Georg Fluegen, Rosa Guglielmi, Rui P. L. Neves, Thilo Hackert, Emrullah Birgin, Stefan A. Cieslik, Monica Sudarsanam, Christiane Driemel, Guus van Dalum, Andre Franken, Dieter Niederacher, Hans Neubauer, Tanja Fehm, Jutta M. Rox, Petra Boehme, Lena Haeberle, Wolfgang Goering, Irene Esposito, Stefan A. Topp, Frank A. W. Coumans, Juergen Weitz, Wolfram T. Knoefel, Johannes C. Fischer, Ulrich Bork, Nuh N. Rahbari
Summary: By employing diagnostic leukapheresis (DLA) to screen large blood volumes, the sensitivity and quantity of circulating tumor cells (CTCs) in pancreatic adenocarcinoma (PDAC) can be improved, while providing ample material for genomic analysis. DLA-derived CS-CTCs can serve as a useful tool for identifying high-risk PDAC patients and optimizing treatment strategies.
Article
Hematology
Stephanie M. Vorholt, Veronika Lenz, Burkhard Just, Juergen Enczmann, Johannes C. Fischer, Peter A. Horn, Thomas A. Zeiler, Vera Balz
Summary: This study reports the molecular findings of the JK blood group, including allele frequencies and antigen prevalence data. The results demonstrate that genotyping tests can effectively identify compatible blood products for different populations, reducing the risk of transfusion reactions.
TRANSFUSION MEDICINE AND HEMOTHERAPY
(2023)
Article
Medicine, Research & Experimental
Johannes C. Fischer, Vera Balz, Danny Jazmati, Edwin Boelke, Noemi F. Freise, Verena Keitel, Torsten Feldt, Bjoern-Erik Ole Jensen, Johannes Bode, Tom Luedde, Dieter Haeussinger, Ortwin Adams, E. Marion Schneider, Juergen Enczmann, Jutta M. Rox, Derik Hermsen, Karin Schulze-Bosse, Detlef Kindgen-Milles, Wolfram Trudo Knoefel, Martijn van Griensven, Jan Haussmann, Balint Tamaskovics, Christian Plettenberg, Kathrin Scheckenbach, Stefanie Corradini, Alessia Pedoto, Kitti Maas, Livia Schmidt, Olaf Grebe, Irene Esposito, Anja Ehrhardt, Matthias Peiper, Bettina Alexandra Buhren, Christian Calles, Andreas Stoehr, Peter Arne Gerber, Artur Lichtenberg, Hubert Schelzig, Yechan Flaig, Amir Rezazadeh, Wilfried Budach, Christiane Matuschek
Summary: HLA haplotypes are associated with the severity and duration of COVID-19, and these associations are also influenced by sex and CCRR5 mutation.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2022)
Article
Hematology
Shanmuganathan Chandrakasan, Sharat Chandra, Chengyu Prince, Lisa J. Kobrynski, Laura Lucas, Kiran Patel, Jolan Walter, Rebecca H. Buckley, Roland Meisel, Sujal Ghosh, Suhag H. Parikh
Summary: This study investigated the immune status of CD40 ligand (CD40L)-deficient carriers and the outcomes of hematopoietic stem cell transplantation (HSCT) using them as donors for CD40L-deficient patients. The results showed that the immune profiles of carriers were comparable to healthy controls, and HSCT using carriers as donors resulted in excellent immune reconstitution without immune dysregulation. The study also demonstrated higher CD40L expression in the T-follicular helper (Tfh) compartment of carriers, suggesting a potential advantage for CD40L-expressing Tfh cells.
Article
Medicine, Research & Experimental
Noemi F. Freise, Milena Kivel, Olaf Grebe, Christian Meyer, Bahram Wafaisade, Matthias Peiper, Tobias Zeus, Jan Schmidt, Judith Neuwahl, Danny Jazmati, Tom Luedde, Edwin Boelke, Torsten Feldt, Bjoern Erik Ole Jensen, Johannes Bode, Verena Keitel, Jan Haussmann, Balint Tamaskovics, Wilfried Budach, Johannes C. Fischer, Wolfram Trudo Knoefel, Marion Schneider, Peter Arne Gerber, Alessia Pedoto, Dieter Haeussinger, Martijn van Griensven, Amir Rezazadeh, Yechan Flaig, Julian Kirchner, Gerald Antoch, Hubert Schelzig, Christiane Matuschek
Summary: This study presents a case series of eight patients who developed cardiac symptoms shortly after receiving SARS-CoV-2 mRNA vaccines. Three patients required hospitalization, but all patients fully recovered within seven days. The results suggest that cardiac adverse events such as myocarditis or pericarditis after mRNA vaccination are rare but possible.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2022)