Journal
JOURNAL OF PROTEOME RESEARCH
Volume 15, Issue 5, Pages 1639-1648Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.6b00103
Keywords
influenza A virus; NS1; interactome; PRP19; RNA splicing/processing
Categories
Funding
- Ministry of Science and Technology (MOST), Taiwan [102-2320-B-182-029-MY3, 103-2325-B-182-007, 103-2632-B-182-001]
- Chang Gung Memorial Hospital (CGMH), Taiwan [CLRPD190015, CMRPD2B0053, BMRPC77]
- MOST [103-2321-B-182-011, 104-2321-B-182-003]
- CGMH, Taiwan [CMRPD1E044-1 3, BMRPC09]
Ask authors/readers for more resources
Influenza A virus, which can cause severe respiratory illnesses in infected individuals, is responsible for worldwide human pandemics. The NS1 protein encoded by this virus plays a crucial role in regulating the host antiviral response through various mechanisms. In addition, it has been reported that NS1 can modulate cellular pre-mRNA splicing events. To investigate the biological processes potentially affected by the NS1 protein in host cells, NS1-associated protein complexes in human cells were identified using coimmunoprecipitation combined with GeLC-MS/MS. By employing software to build biological process and protein protein interaction networks, NS1-interacting cellular proteins were found to be related to RNA splicing/processing, cell cycle, and protein folding/targeting cellular processes. By monitoring spliced and unspliced RNAs of a reporter plasmid, we further validated that NS1 can interfere with cellular pre-mRNA splicing. One of the identified proteins, pre-mRNA-processing factor 19 (PRP19), was confirmed to interact with the NS1 protein in influenza A virus-infected cells. Importantly, depletion of PRP19 in host cells reduced replication of influenza A virus. In summary, the interactome of influenza A virus NS1 in host cells was comprehensively profiled, and our findings reveal a novel regulatory role for PRP19 in viral replication.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available