Article
Agronomy
Ni Zhang, Weiwen Tan, Guimei Luo, Tianyi Pu, Jinqiu Wang, Xianhu Zhang, Yuehua Song
Summary: Camellia tetracocca, a unique tea species in southwest China, is frequently attacked by the pest Empoasca onukii. The metabolic mechanisms of C. tetracocca's defense against this pest are still unclear. Through comparative analysis using GC-MS and LC-MS, differential metabolites were identified, showing that accumulation of specialized metabolites may contribute to the resistance of C. tetracocca against E. onukii. These specialized metabolites have the potential to be used as biocontrol agents against the pest.
Article
Agriculture, Multidisciplinary
Bo-Min Kim, Hyeon-Jeong Lee, Yeong H. Song, Hyun-Jin Kim
Summary: Increasing NaCl concentration led to decreased growth and mineral imbalance in spinach plants. NaCl irrigation resulted in decreased levels of most metabolites, affecting the nutritional quality of spinach.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Chemistry, Applied
Mengyu Chen, Wenjie Huang, Zhibin Yin, Wenyang Zhang, Qian Kong, Shaowen Wu, Wenyan Li, Zhao Bai, Alisdair R. Fernie, Xiaodan Huang, Shijuan Yan
Summary: The research found metabolic differences among black wolfberry fruits grown in different geographical regions, with some samples showing significantly different metabolite profiles with higher levels of beneficial components, providing a new direction for further studies on the health benefits of black wolfberry fruits.
Article
Chemistry, Applied
Wenliang Wu, Meiling Lu, Jiakun Peng, Haipeng Lv, Jiang Shi, Shuguang Zhang, Zhen Liu, Jihua Duan, Dan Chen, Weidong Dai, Zhi Lin
Summary: This study investigated the chemical characteristics of Jianghua Kucha (JHKC), a special tea germplasm in China, and found that it mainly contains high levels of purine alkaloids and methylated flavanols, as well as low levels of flavonol glycosides. Caffeine metabolism, flavonoid biosynthesis, and flavonol and flavone biosynthesis were identified as the most differential metabolic pathways. JHKC can be a valuable resource for breeding tea cultivars with high contents of theacrine, non-epi-form flavanols, and methylated flavanols.
Article
Chemistry, Applied
Wen-qiang Cai, Cai-yan Jiang, Shan Shang, Shu-chen Wang, Kai-yue Zhu, Xiu-ping Dong, Da-yong Zhou, Peng-fei Jiang
Summary: Sea urchin gonads have high nutritional value and suffer from rapid degeneration during storage. This study aims to identify biochemical indicators for assessing the freshness of sea urchin gonads. The dominant genera and metabolites of sea urchin gonads were found to change, with specific metabolic pathways enriched in different types of metabolites. The growth of the dominant genus Aliivibrio significantly influenced the production of differential metabolites. These findings provide valuable information for accurately determining the freshness and shelf life of sea urchin gonads.
Article
Plant Sciences
Chen Zhu, Shuting Zhang, Chengzhe Zhou, Siyi Xie, Guangwu Chen, Caiyun Tian, Kai Xu, Yuling Lin, Zhongxiong Lai, Yuqiong Guo
Summary: The study identified 34 m6A regulatory genes in tea plants, whose expansion was mainly driven by gene duplications and evolved through purifying selection. Sequence variation in gene structure contributed to functional diversification of m6A regulatory genes, with most of them showing differential expression under environmental stresses and tea-withering stage. Additionally, a negative feedback loop between RNA methylation and DNA methylation was found by interacting with each other's methylation regulatory genes.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Food Science & Technology
Kai Xu, Caiyun Tian, Chengzhe Zhou, Chen Zhu, Jingjing Weng, Yun Sun, Yuling Lin, Zhongxiong Lai, Yuqiong Guo
Summary: In this study, the characteristic metabolites and volatile compounds of Rougui Wuyi Rock tea from different culturing regions were analyzed using metabolomics, aroma activity value method, and chemometrics. The results showed significant differences in flavor qualities, especially in taste and aroma, among the different Rougui WRTs.
Article
Chemistry, Applied
Ting Zeng, Baohua Fang, Fenglin Huang, Li Dai, Zhi Tang, Jinglin Tian, Guodong Cao, Xuanlin Meng, Yuanchen Liu, Bo Lei, Minghua Lu, Zongwei Cai
Summary: Through metabolomics analysis, it was found that under cadmium stress, most carbohydrates and amino acids were down-regulated as the cadmium concentration increased in rice grains, except for myoinositol which can prevent cadmium toxicity. D-Mannitol and L-cysteine were up-regulated in low-cadmium-accumulating rice with the increase of cadmium concentration. Organic acids, especially 13-(S)-hydroperoxy-9(Z),11(E),15(Z)-octadecatrienoic acid related to alphalinolenic acid metabolism and jasmonic acid production, were activated.
Article
Biochemistry & Molecular Biology
Fei Yan, Dong Qu, Xiaohua Chen, Haitao Zeng, Xinsheng Li, Ching Yuan Hu
Summary: This study revealed the metabolic responses of tea plants to cold stress through metabolomics analysis. Exogenous application of ALA was found to alter the metabolism of tea plants and enhance their cold resistance. The research provides a basis for the exogenous usage of ALA to protect tea plants from cold stress.
Article
Horticulture
Jung-Sung Chung, Hyeong-Cheol Kim, Su-Min Yun, Hyun-Jin Kim, Cheol-Soo Kim, Jeung-Joo Lee
Summary: Sulfur is an essential nutrient for plant growth and metabolism, and significantly affects the growth and metabolites of lettuce. Higher sulfur concentration treatment resulted in increased growth and metabolite content in lettuce compared to lower sulfur concentration treatment.
Article
Biochemical Research Methods
Martin Meyer, Lidia Montero, Sven W. Meckelmann, Oliver J. Schmitz
Summary: This study compared the analysis of carbohydrates using four common chromatographic methods coupled to mass spectrometry. It was found that gas chromatography and reversed-phase liquid chromatography, after derivatisation, showed superior separation performance compared to the other methods. Targeted analysis using different working modes of the mass spectrometer improved sensitivity and repeatability, with RP-LC-MS in MRM mode after derivatisation being the most suitable method for monosaccharide analysis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Poonam Kulyal, Satyabrata Acharya, Aditya B. Ankari, Praveen K. Kokkiripati, Sarada D. Tetali, Agepati S. Raghavendra
Summary: The phytochemical profiles of seven cultivars of Curcuma longa L. and C. aromatica Salisb. were compared, revealing significant variability. A total of 39 new compounds were detected, some of which have health benefits.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Environmental Sciences
Yueyue Li, Yashu Li, Guanxi Wang, Julin Li, Meihua Zhang, Jingyao Wu, Cheng Liang, Huayun Zhou, Jianxia Tang, Guoding Zhu
Summary: Insecticide resistance is a severe global problem for vector control. A recent study analysed the molecular metabolite changes in deltamethrin-resistant An. sinensis mosquitoes using liquid chromatography tandem mass spectrometry (LC-MS/MS) and found that the differential metabolites were mainly associated with glycerophospholipid metabolism. These findings provide further mechanistic understanding of insecticide resistance in An. sinensis.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Pharmacology & Pharmacy
Jigni Mishra, Washim Khan, Sayeed Ahmad, Kshipra Misra
Summary: Supercritical fluid extraction using carbon dioxide was utilized to extract Cordyceps sinensis, resulting in high purity extracts. Characterization using HPTLC and GC-MS confirmed the presence of various compounds in the extracts. Chemometric analysis illustrated interrelatedness between different extracts.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Agronomy
Archis Pandya, Bjorn Thiele, Andres Zurita-Silva, Bjorn Usadel, Fabio Fiorani
Summary: The study evaluated saponin content and their corresponding sapogenins in 114 different quinoa accessions, showing considerable variability in saponin content in quinoa. Approximately 75% of the genotypes were classified as low-saponin content lines, which is promising for current and future quinoa breeding programs. Additionally, twelve saponins of the oleanane type were identified, including a previously unreported compound, contributing to the valorization of genetic resources for quinoa.