Review
Cell Biology
Amaris Guevara -Garcia, Matis Soleilhac, Nicolas Minc, Delphine Delacour
Summary: Epithelial cells play a key role in tissue organization in multicellular organisms. In developing epithelial tissues, cellular proliferation and differentiation are tightly regulated to ensure proper organ formation and function. This review focuses on the current understanding of cell division and regulatory mechanisms in the intestinal epithelial tissue, and their importance in developmental biology and physiopathology.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Jing Xie, Javad Najafi, Remi Le Borgne, Jean-Marc Verbavatz, Catherine Durieu, Jeremy Salle, Nicolas Minc
Summary: Cells are filled with macromolecules and polymer networks that provide viscous and elastic properties to the cytoplasm. Using magnetic tweezers, researchers found that the cytoplasm can exert reactive forces to move organelles back to their original positions. These findings have important implications for cell division positioning and cellular organization.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Ramiro Tomasina, Fabiana C. Gonzalez, Erica S. Martins-Duarte, Philippe Bastin, Mathieu Gissot, Maria E. Francia
Summary: Centrosomes are crucial for cell division and formation of the mitotic spindle. This study reveals the important role of the inner core protein TgCep250L1 in Toxoplasma gondii cell division, as its absence leads to nuclear segregation defects and failure to assemble the mitotic spindle.
Review
Cell Biology
Imge Ozugergin, Alisa Piekny
Summary: Cytokinesis, the process of physically dividing a cell into two daughters, has been extensively studied in vitro and early embryos, but its regulation in different animal cell types and developmental contexts remains poorly understood. Recent studies have revealed striking differences in the regulation of cytokinesis between different cell types and organisms, including diverse threshold requirements for structural components and different mechanisms of regulation. This review focuses on these differences, particularly in pathways independent of the mitotic spindle, and associated with the cortex, kinetochores, or chromatin.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Joana Soares-de-Oliveira, Helder Maiato
Summary: This study proposes a new perspective that chromosome biorientation is driven by microtubule self-organization at kinetochores, challenging the previous stochastic models.
Review
Cell Biology
Jeremy Salle, Nicolas Minc
Summary: Early cellular patterning is crucial for embryonic development and relies on rapid reductive divisions and cell fate specification. The positioning of cell divisions plays a significant role in cell behavior, cellular decisions, and morphogenesis.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Pingping Dong, Ziqing Cai, Bingfeng Li, Yueqin Zhu, Alice K. Y. Chan, Michael W. L. Chiang, Chun Hang Au, Wing Kin Sung, Tan To Cheung, Chung Mau Lo, Kwan Man, Nikki P. Lee
Summary: The study reveals a novel function of HFE in promoting cytokinesis during mitotic cell division in HCC, independent of its iron-regulating ability. Clinical analysis shows that upregulation of HFE is associated with larger tumors and poor prognosis. The findings lay the groundwork for the development of tumor intolerable therapeutics.
Article
Cell Biology
Alexandre Thomas, Emmanuel Gallaud, Aude Pascal, Laurence Serre, Isabelle Arnal, Laurent Richard-Parpaillon, Matthew Scott Savoian, Regis Giet
Summary: During neuroblast division, asymmetric positioning of the cleavage furrow is achieved through the regulation of centralspindlin, with different pools of centralspindlin competing to determine the final furrow position and cell size asymmetry. Loss of the cortical centralspindlin pool leads to enhanced signaling at the midzone, resulting in furrow repositioning and decreased size asymmetry in neuroblasts.
Article
Oncology
Girish Rajendraprasad, Susana Eibes, Claudia Guasch Boldu, Marin Barisic
Summary: TH588 has been identified as a promising anti-cancer compound that stabilizes microtubules in mitotic cells, leading to mitotic arrest or cell death. Its anticancer properties largely depend on its ability to target microtubules.
Article
Gastroenterology & Hepatology
Wei-Ting Kuo, Li Zuo, Matthew A. Odenwald, Shariq Madha, Gurminder Singh, Christine B. Gurniak, Clara Abraham, Jerrold R. Turner
Summary: The study found that ZO-1 expression was reduced in biopsy specimens from patients with inflammatory bowel disease. Despite mildly increased intestinal permeability, ZO-1(KO.IEC) mice were healthy and did not develop spontaneous disease. However, these mice were hypersensitive to mucosal insults and displayed defective repair.
Article
Biochemistry & Molecular Biology
Fiorella Faienza, Federica Polverino, Girish Rajendraprasad, Giacomo Milletti, Zehan Hu, Barbara Colella, Deborah Gargano, Flavie Strappazzon, Salvatore Rizza, Mette Vixo Vistesen, Yonglun Luo, Manuela Antonioli, Valentina Cianfanelli, Caterina Ferraina, Gian Maria Fimia, Giuseppe Filomeni, Daniela De Zio, Joern Dengjel, Marin Barisic, Giulia Guarguaglini, Sabrina Di Bartolomeo, Francesco Cecconi
Summary: AMBRA1 is a key factor for nervous system development, primarily associated with autophagy and cell proliferation control. This study reveals that AMBRA1 is phosphorylated during mitosis and is critical for spindle function and orientation, driven by NUMA1 protein. The localization and dynamics of NUMA1 are dependent on AMBRA1 presence, phosphorylation, and binding ability. These findings suggest an additional role of AMBRA1 in tissue morphogenesis and differentiation, which could have implications for development and cancer oncogenesis.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Physiology
Mathew Bloomfield, Jing Chen, Daniela Cimini
Summary: This study found that differences in mitotic duration can be explained by variations in spindle microtubule densities and sizes of the cell, spindle, and spindle poles. The study suggests that spindle size does not always scale with cell size in mammalian cells, and cell size alone is not sufficient to explain differences in metaphase duration. Only when considering a number of spindle architectural features along with cell size can the kinetics of SAC silencing, and hence mitotic duration, be explained in different clones.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biology
Valentina Stimac, Isabella Koprivec, Martina Manenica, Juraj Simunic, Iva M. Tolic
Summary: The augmin complex is essential for the formation of correctly aligned microtubule structures during mitosis, preventing erroneous attachments between sister kinetochores.
Article
Multidisciplinary Sciences
Shuzhen Yang, Mengya Cai, Junjie Huang, Shengnan Zhang, Xiaoli Mo, Kai Jiang, Huiting Cui, Jing Yuan
Summary: Faithful chromosome segregation of duplicated genomes is crucial for male gametogenesis and mosquito transmission of Plasmodium. This study identifies a parasite-specific EB1 protein that has both MT-lattice affinity and plays a crucial role in regulating spindle-kinetochore attachment during male gametogenesis. Loss of EB1 results in defective mosquito transmission due to impaired spindle-kinetochore attachment in Plasmodium.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Yukihiro Sera, Tsuneo Imanaka, Masafumi Yamaguchi
Summary: SDS is a genetic disorder caused by mutations in the SBDS gene, which is involved in ribosome biogenesis. The interaction between SBDS and RNF2 at different stages of the cell cycle may play a role in mitotic progression.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Xiaoyu Xia, Yu-Chen Lo, Ankur A. Gholkar, Silvia Senese, Joseph Y. Ong, Erick F. Velasquez, Robert Damoiseaux, Jorge Z. Torres
ACS CHEMICAL BIOLOGY
(2019)
Review
Biochemistry & Molecular Biology
Joseph Y. Ong, Jorge Z. Torres
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Editorial Material
Cell Biology
Jorge Z. Torres
MOLECULAR BIOLOGY OF THE CELL
(2019)
Article
Biochemistry & Molecular Biology
Ankur A. Gholkar, Stefan Schmollinger, Erick F. Velasquez, Yu-Chen Lo, Whitaker Cohn, Joseph Capri, Harish Dharmarajan, William J. Deardorff, Lucy W. Gao, Mai Abdusamad, Julian P. Whitelegge, Jorge Z. Torres
Review
Biochemistry & Molecular Biology
Joseph Y. Ong, Jorge Z. Torres
Review
Cell Biology
Joseph Y. Ong, Michelle C. Bradley, Jorge Z. Torres
Article
Cell Biology
Ivan Ramirez, Ankur A. Gholkar, Erick F. Velasquez, Xiao Guo, Bobby Tofig, Robert Damoiseaux, Jorge Z. Torres
Summary: This study identifies a previously uncharacterized myosin RLC, Myl5, as a critical player in cell division, with depletion of Myl5 leading to defects in mitotic spindle assembly, chromosome segregation, and a slower transition through mitosis. Myl5 associates with the mitotic spindle and interacts with MYO10, suggesting its importance in cell division may be mediated through MYO10.
Article
Biochemical Research Methods
Yenni A. Garcia, Erick F. Velasquez, Lucy W. Gao, Ankur A. Gholkar, Kevin M. Clutario, Keith Cheung, Taylor Williams-Hamilton, Julian P. Whitelegge, Jorge Z. Torres
Summary: **The spindle assembly checkpoint (SAC) is crucial for detecting errors in microtubule-kinetochore attachments and tension, which can lead to chromosome segregation errors linked to diseases like cancer. Despite advances in understanding SAC composition and regulatory factors, the proximity associations of core SAC components have not been systematically explored. Using a BioID2-proximity labeling proteomic approach, the proximity protein environment of five core SAC proteins has been characterized in mitotic-enriched cell populations where the SAC is active. The analysis has not only validated known SAC complexes and protein-protein interactions, but also revealed new protein associations that provide insight into SAC function and suggest future research directions for better understanding.**
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Rebeccah A. Warmack, Eric Z. Pang, Esther Peluso, Jonathan D. Lowenson, Joseph Y. Ong, Jorge Z. Torres, Steven G. Clarke
Summary: This study identified a potential E3 ubiquitin ligase adaptor protein, PCMTD1, which can interact with Cullin-RING proteins and may provide an alternative pathway for maintaining modified proteins.
Article
Biochemistry & Molecular Biology
Xiao Guo, Ivan Ramirez, Yenni A. Garcia, Erick F. Velasquez, Ankur A. Gholkar, Whitaker Cohn, Julian P. Whitelegge, Bobby Tofig, Robert Damoiseaux, Jorge Z. Torres
Summary: This study identifies the importance of dual-specificity phosphatase 7 (DUSP7) in regulating chromosome alignment during mitosis by regulating the levels of active phospho-ERK2. Overexpression of DUSP7 or knockdown of ERK2 results in chromosome misalignment or defective chromosome congression during mitosis, supporting a model where MEK and DUSP7 cooperatively regulate cell division.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Endocrinology & Metabolism
Mark P. Sawicki, Ankur A. Gholkar, Jorge Z. Torres
