Article
Cell Biology
Alex G. Batrouni, Nirmalya Bag, Henry T. Phan, Barbara A. Baird, Jeremy M. Baskin
Summary: Phosphatidylinositol 4-kinase III alpha (PI4KIII alpha) is responsible for generating phosphatidylinositol (4)-phosphate at the plasma membrane, and the differential palmitoylation patterns of its anchor protein EFR3B control its distribution and phosphoinositide homeostasis.
JOURNAL OF CELL SCIENCE
(2022)
Article
Chemistry, Physical
Soohyung Park, Ilya Levental, Richard W. Pastor, Wonpil Im
Summary: The affinity of TM proteins for ordered membrane phases is influenced by lipidation, TM length, and lipid accessible surface area. In this study, the raft affinities of a TM domain and its depalmitoylated variant were assessed using free energy simulations. The results showed that both peptides preferentially partition into the liquid disordered phase and the detailed substructure of the liquid ordered phase modulates peptide partitioning.
JOURNAL OF CHEMICAL THEORY AND COMPUTATION
(2023)
Article
Multidisciplinary Sciences
Tomaz Zagar, Miha Pavsic, Aljaz Gaber
Summary: EpCANI is a cell-surface protein utilized as a carcinoma marker, involved in nuclear signaling via RIP. Research has shown that the oligomeric state of EpCAM is a determinant in RIP, with the disulfide-stabilized dimer being more resistant to cleavage than the monomeric form.
Article
Biology
Lea Antje Adolf, Angelika Mueller-Jochim, Lara Kricks, Jan-Samuel Puls, Daniel Lopez, Fabian Grein, Simon Heilbronner, Bavesh D. Kana
Summary: Sufficient access to transition metals such as iron is essential for bacterial proliferation and their active limitation within host tissues effectively restricts infection. A study on Staphylococcus aureus reveals that the iron-regulated surface determinant (Isd) system is used by the pathogen to acquire hemoglobin-derived heme, and the heme-specific permease IsdF is energized by the general ATPase FhuC. The study also shows that IsdF requires appropriate location within the membrane and is associated with functional membrane microdomains, including the scaffolding protein flotillin A (FloA), which is essential for Isd-dependent bacterial growth.
Review
Biochemistry & Molecular Biology
Keith T. Woodley, Mark O. Collins
Summary: Protein palmitoylation is important in cellular processes, with the palmitoyltransferase ZDHHC5 being a unique member of the PAT family that plays a vital role in different cell types. Recent research has revealed several mechanisms to control the activity, localization, and function of ZDHHC5.
Article
Biology
Jasmin Mertins, Jerome Finke, Ricarda Sies, Kerstin M. Rink, Jan Hasenauer, Thorsten Lang
Summary: SNARE proteins, such as syntaxin 1A and SNAP25, are seen to be largely separated in the plasma membrane but can be organized on a larger scale through interactions at the SNARE domain level. This suggests that SNARE domain interactions are not only involved in membrane fusion at the nanoscale, but also play a crucial role in organizing proteins on the mesoscale.
Review
Biology
Elizabeth L. Kordyum, Olga A. Artemenko, Karl H. Hasenstein
Summary: The necessity of including plants in a Bioregenerative Life Support System has led to research in optimizing plant growth facilities and better understanding the role of plant cell membranes in signaling, transport, and sensing. The composition of the cell membrane, particularly the recruitment of specific proteins, affects membrane domains and plasmodesmata. Further research is needed to explore the role of lipid rafts in physiological processes.
Article
Cell Biology
Nazli Ezgi Ozkan, Berfu Nur Yigit, Beste Senem Degirmenci, Mohammad Haroon Qureshi, Gamze Nur Yapici, Altug Kamacioglu, Nima Bavili, Alper Kiraz, Nurhan Ozlu
Summary: Cell division requires reorganization of the cell cortex, in which PCDH7 and ZDHHC5 play a role through their interaction and palmitoylation. The localization of PCDH7 depends on the palmitoylation activity of ZDHHC5. Silencing PCDH7 leads to abnormal cell division and affects the reorganization of the cell cytoskeleton.
JOURNAL OF CELL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Hironobu Hojo, Toshiki Takei, Yuya Asahina, Nobuaki Okumura, Toshifumi Takao, Masatomo So, Isao Suetake, Takeshi Sato, Akihiro Kawamoto, Yoshio Hirabayashi
Summary: In this study, the essential protein Caveolin-1 for caveola formation was chemically synthesized and successfully inserted into the lipid bilayer to form a V-shaped structure. The solid-phase method was used to synthesize peptide segments, and O-acyl isopeptide structures were incorporated to improve solubility. After ligation by the thioester method and the introduction of palmitoyl groups, the obtained polypeptide was inserted into bicelles.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemical Research Methods
Sachio Suzuki, Masaru Yoshikawa, Shunsuke Sawada, Neal K. Devaraj, Shinya Tsukiji
Summary: Conjugating small-molecule ligands to synthetic motifs that can localize to specific organelles or membranes is a practical approach to manipulate biological processes. We have developed a synthetic myristoyl-DCys motif that can undergo S-palmitoylation and localize to the Golgi surface. By miniaturizing the myristoyl-DCys motif, we have identified four new derivatives that retain the palmitoylation-dependent Golgi localization property. These miniaturized motifs can be used to generate small-molecule ligand conjugates for protein localization and cell signaling control.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Ritika Tewari, Bieerkehazhi Shayahati, Ying Fan, Askar M. Akimzhanov
Summary: The study identified previously uncharacterized lipidation of ZAP-70, which is crucial for its interaction with protein substrates in the TCR signaling pathway. While this posttranslational modification does not affect its enzymatic activity, it plays a critical role in T cell activation and function. The findings suggest that TCR-induced S-acylation of ZAP-70 serves as a vital regulator of T cell-mediated immunity.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Microbiology
Lin Lv, Li Chai, Jie Wang, Mengge Wang, Danhui Qin, Hui Song, Yue Fu, Chunyuan Zhao, Jihui Jia, Wei Zhao, Mutian Jia
Summary: In this study, we identified a transmembrane selenoprotein, SELENOK, located in the endoplasmic reticulum (ER), which is induced during viral infection and enhances innate immune response against herpes simplex virus-1 (HSV-1) by promoting STING oligomerization. SELENOK interacts with STING, facilitating its translocation from the ER to the Golgi. Thus, controlling STING activation through selenium-mediated SELENOK expression could be a potential therapeutic strategy for STING-associated diseases.
Article
Multidisciplinary Sciences
Yiyang Jiang, Thomas L. Benz, Stephen B. Long
Summary: The study provides structural insights into the mechanism by which HHAT facilitates the palmitoylation of Hedgehog proteins, and may contribute to the development of cancer inhibitors. The structures reveal how HHAT deals with substrates of different properties to catalyze the reaction, and have implications for related enzymes catalyzing acylation of other molecules.
Article
Biochemistry & Molecular Biology
Jozsef Gal, Vimala Bondada, Charles B. Mashburn, David W. Rodgers, Dorothy E. Croall, James W. Geddes
Summary: CAPN5 is a calcium-activated neutral thiol protease that is partly membrane associated. S-acylation of CAPN5 is critical for its membrane localization and enzymatic activity.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Article
Multidisciplinary Sciences
Claudia Roessler, Julia Schumann
Summary: Inflammation is influenced by the adaptation of macrophages and endothelial cells, which can be dysregulated in both acute and chronic diseases. The interaction between these cells and dietary components, such as polyunsaturated fatty acids (PUFA), can impact their differentiation processes. RNA sequencing analysis was used to study the global changes in gene expression during cell differentiation and uncover the molecular mechanisms underlying the effects of PUFA on macrophages and endothelial cells. This dataset provides a valuable resource for studying the transcriptional and post-transcriptional changes associated with inflammation and the modulation by omega-3 and omega-6 fatty acids.
Article
Biochemistry & Molecular Biology
Ya-Fei Jing, Hao-Xun Wei, Fang-Fang Liu, Yi-Fan Liu, Lei Zhou, Jin-Feng Liu, Shi-Zhong Yang, Hui-Zhan Zhang, Bo-Zhong Mu
Summary: Surfactin, a promising compound for various industries, has low production yield in wild strains. In this study, the overexpression of eight genes related to fatty acid biosynthesis pathway increased the surfactin yield of engineered strains. The conversion of acetyl-CoA to malonyl-CoA was identified as the rate-limiting step in fatty acid biosynthesis. Additionally, modifications in the surfactin isoforms were observed, indicating the potential for pathway engineering. Deletion of the lpdV gene led to a significant decrease in surfactin production, highlighting the importance of branched-chain fatty acid biosynthesis. This work enables the design of more efficient engineered strains for surfactin production and expands its industrial applications.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Chemistry, Applied
Fang-Hui Liu, Cheng-Long Gao, Cui-Cui Zhang, Hong-Ze Gang, Bo-Zhong Mu, Shi-Zhong Yang
Summary: In this study, a bio-based zwitterionic surfactant called PADS was synthesized through epoxidation and acetalization reactions, and it was found to exhibit excellent interfacial activity and potential for low-dose application. Furthermore, PADS maintained good interfacial activity and ultra-low oil-water interfacial tension even under high salt concentration conditions. This research provides a new method for preparing bio-based surfactants with high interfacial activity.
JOURNAL OF SURFACTANTS AND DETERGENTS
(2023)
Article
Environmental Sciences
Lei Zhou, Jun Wu, Jia-Heng Ji, Jie Gao, Yi-Fan Liu, Biao Wang, Shi-Zhong Yang, Ji-Dong Gu, Bo-Zhong Mu
Summary: This study investigated the microbial communities and functional groups in production water from five petroleum reservoirs in China using chemical and molecular biological analyses. The dominant phyla in these reservoirs were Proteobacteria, Deferribacterota, Firmicutes, Desulfobacterota, Euryarchaeota, and Thermoplasmatota. The corrosion rates in the production water were higher than controls and were positively correlated with the abundances of sulfate-reducing prokaryotes (SRP). The SRP diversity analysis showed differences between onshore and offshore oilfields, and the core SRP taxa varied based on temperature.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Multidisciplinary
Jian-Qiao Lang, Homely Isaya Mtui, Hong-ze Gang, Bo-Zhong Mu, Shi-Zhong Yang
Summary: The salt-tolerant and low-toxicity biobased zwitterionic surfactant SPBOPA has excellent interfacial properties and enhanced oil recovery effects, even in high temperature and high salt environments. It also exhibits high biodegradability and strong antidilution and antiadsorption properties.
Article
Energy & Fuels
Xin-Ning Bao, Ying-Cheng Li, Wei-Dong Zhang, Hui Zhang, Hong-Ze Gang, Jun Jin, Shi-Zhong Yang, Ou Sha, Bo-Zhong Mu
Summary: A new alkyl polyether benzyl sulfonate anionic surfactant, named sodium iso-tridecanol polyoxyethylene ether benzyl sulfonate, was synthesized with ultralow interfacial tensions at high salinity and high temperatures. Its structure was confirmed by 1H NMR. The surfactant showed strong interfacial activity and excellent phase behavior with high solubilization parameters, indicating its potential for enhanced oil recovery in high temperature and high salinity oil reservoirs.
Article
Green & Sustainable Science & Technology
Shan Yuan, Hong-Ze Gang, Yi-Fan Liu, Lei Zhou, Muhammad Irfan, Shi-Zhong Yang, Bo-Zhong Mu
Summary: CO2 geological sequestration in subsurface shale formations is a promising strategy that can store CO2 and enhance shale gas production. The study on gas adsorption and diffusion behavior on different types of kerogens reveals that CO2 has higher capacities than CH4, and the adsorption and diffusion capabilities decrease with increasing water content, while the CO2/CH4 adsorption selectivity increases. Type IIIA kerogen demonstrates the best potential for adsorption and diffusion.
Article
Biochemistry & Molecular Biology
Jing Zhou, Si-Jia Miao, Shi-Zhong Yang, Jin-Feng Liu, Hong-Ze Gang, Bo-Zhong Mu
Summary: A new sensitive method for the quantitative analysis of rhamnolipid was developed based on a simple derivatization reaction. The established method showed good reproducibility and accuracy, and was successfully applied to the quantitative analysis of rhamnolipid homologs.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Chemistry, Physical
Hong-ze Gang, Qing-ying Wu, Zhi-yuan Yang, Zhi-qing Su, Ying-cheng Li, Shi-zhong Yang, Bo-Zhong Mu
Summary: The adsorption kinetics of surfactin homologues at the n-hexadecane/aqueous solution interface was investigated using droplet-based microfluidic method. Molecular dynamics simulations showed that the adsorption energy of surfactin depends on its structure. Experimental results demonstrated that shorter chain surfactin, such as C13-surfactin and C14-surfactin, had faster adsorption and desorption rate constants. The study also revealed that C15-surfactin had a stronger tendency to adsorb at the interface due to its higher adsorption energy and aggregation behavior in solution.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Chemistry, Physical
Shan Yuan, Hong-Ze Gang, Yi-Fan Liu, Lei Zhou, Muhammad Irfan, Shi-Zhong Yang, Bo-Zhong Mu
Summary: In this study, simulations were conducted to investigate the behavior of CO2 and CH4 adsorption and diffusion on kerogen models with different maturity. It was found that CO2 has a higher adsorption capacity compared to CH4, and this capacity increases with maturity. Furthermore, the addition of water reduces the adsorption and diffusion coefficients of both gases but increases the selectivity of CO2 over CH4. These findings suggest that injecting CO2 into shale gas and oil formations can enhance energy recovery.
MOLECULAR SIMULATION
(2023)
Article
Green & Sustainable Science & Technology
Wei Wang, Ming-Yue Liang, Jian-Qiao Lang, Homely Isaya Mtui, Shi-Zhong Yang, Bo-Zhong Mu
Summary: A new bio-based zwitterionic surfactant with strong interfacial activity at high temperature was developed through the modification of renewable non-edible oils. The synthetic route was optimized to improve the interfacial activity of the final product by controlling and monitoring the residues of intermediates. This study confirms that the removal of residual intermediates and promotion of the conversion rate contribute to enhancing the interfacial activity and provides insights for the design of sustainable alternatives in enhanced oil recovery.
Article
Chemistry, Multidisciplinary
Jia-Yi Li, Yi-Fan Liu, Lei Zhou, Hong-Ze Gang, Jin-Feng Liu, Gang-Zheng Sun, Wei-Dong Wang, Shi-Zhong Yang, Bo-Zhong Mu
Summary: In this study, a new strain, Geobacillus thermodenitrificans ME63, capable of degrading hydrocarbons in thermophilic and facultative environments and producing biosurfactants, was isolated from an oilfield. The composition, chemical structure, and surface activity of the biosurfactant produced by G. thermodenitrificans ME63 were investigated. The biosurfactant was identified as surfactin with six variants, which exhibited excellent resistance to temperature, salinity, and pH changes in terms of its surface activity and emulsification properties.
Article
Biotechnology & Applied Microbiology
Li-Bin Shou, Zhao-Wei Hou, Yi-Fan Liu, Ying Wang, Lei Zhou, Jin-Feng Liu, Shi-Zhong Yang, Ji-Dong Gu, Xiao-Lin Wu, Bo-Zhong Mu
Summary: This study investigates the effect of air injection on microbial composition and transcriptional activity in oil reservoirs. The researchers found that Firmicutes, specifically the Bacillus genus, were the dominant active bacteria in all samples and their proportion increased with higher gas/liquid ratios. As the gas-to-liquid ratios increased, the microbes showed higher overall transcription activities and propionate became an important metabolite. This research provides theoretical guidance for activating endogenous oil displacement microorganisms and offers insights for the industrial exploitation of depleted reservoirs and extending the development life of oil reservoirs.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2023)
Article
Engineering, Chemical
Homely Isaya Mtui, Fang-Hui Liu, Wei Wang, Jian-Qiao Lang, Shi-Zhong Yang, Bo-Zhong Mu
Summary: A new type of ultra-long chain zwitterionic surfactant, NDAS, with strong interfacial activity and good compatibility with salts and temperatures has been developed through a simple and effective method. This work is significant for the development of sustainable bio-based surfactants and expanding their applications.
GREEN CHEMICAL ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Wan-Qi Qin, Dan Fei, Lei Zhou, Yu-Jia Guo, Shun An, Ou-Hang Gong, Yun-Yang Wu, Jin-Feng Liu, Shi-Zhong Yang, Bo-Zhong Mu
Summary: A new member of the surfactin family, surfactin-C-17, has been isolated from Bacillus subtilis TD7 and its primary chemical structure has been determined. Surfatin-C-17 showed a very low CMC and surface tension, as well as strong antibacterial and hemolytic activities. This study suggests that increasing the hydrophobic chain length of surfactin can lead to improved surfactant properties with potential applications in various industries.
NEW JOURNAL OF CHEMISTRY
(2023)
Review
Microbiology
Asma Sani, Wan-Qi Qin, Jia-Yi Li, Yi-Fan Liu, Lei Zhou, Shi-Zhong Yang, Bo-Zhong Mu
Summary: This article summarizes the structural diversity of microbial lipopeptide biosurfactants and their applications as biocontrol agents in plants. Lipopeptides have low toxicity and wide range of applications, making them important in the pesticide industry.
MICROBIOLOGICAL RESEARCH
(2024)
