Article
Multidisciplinary Sciences
Steven G. Harrellson, Michael S. DeLay, Xi Chen, Ahmet-Hamdi Cavusoglu, Jonathan Dworkin, Howard A. Stone, Ozgur Sahin
Summary: Hygroscopic biological matter in plants, fungi and bacteria plays a significant role in Earth's biomass. These materials exchange water with the environment, exhibit similar mechanical behaviors controlled by the hydration force, and can give rise to a distinct class of solid matter called "hydration solid".
Article
Microbiology
Anella Saggese, Giovanni Di Gregorio Barletta, Maria Vittoria, Giuliana Donadio, Rachele Isticato, Loredana Baccigalupi, Ezio Ricca
Summary: The CotG protein in the Bacillus subtilis spore coat controls spore permeability through its central region of positively charged tandem repeats. The presence of central repeats is a common feature in CotG-like proteins in most Bacillus species. This study contributes to the understanding of the structure and function of bacterial spores.
Article
Biochemistry & Molecular Biology
Charlotte Da Silva, Camille Lamarche, Carole Pichereaux, Emmanuelle Mouton-Barbosa, Gauthier Demol, Sebastien Boisne, Etienne Dague, Odile Burlet-Schiltz, Flavien Pillet, Marie-Pierre Rols
Summary: Low-energy electron beams (LEEB) are a safe and practical sterilization solution for industrial applications. To address the limitations of LEEB, we developed a low-energy pulsed electron beam generator (LEPEB) that can effectively and efficiently eradicate bacteria in a wide range of industrial applications.
BIOELECTROCHEMISTRY
(2024)
Article
Food Science & Technology
Yaru Pang, Ruobin Wu, Tianlin Cui, Zequn Zhang, Li Dong, Fang Chen, Xiaosong Hu
Summary: This study investigated a method of Bacillus subtilis spore inactivation using high pressure, moderate temperature, and the addition of antimicrobial peptide LK. The spores showed cortex hydrolysis and inner membrane damage, leading to a significant reduction in spore counts. Proteomics analysis revealed that the treatment induced changes in the abundance of specific proteins involved in genetic information processing, metabolism, cellular processes, and environmental information processing.
Review
Biochemistry & Molecular Biology
Rachele Isticato
Summary: Mucosal vaccines have advantages such as inducing adaptive immunity and needle-less vaccinations, but their development is currently limited. Innovative antigens, efficient adjuvants, and delivery systems are crucial for effective mucosal vaccines. Bacterial spores have shown promise as antigen vehicles due to their unique structure and ability to induce protective immune responses when mucosally administered.
Review
Biochemistry & Molecular Biology
Anella Saggese, Loredana Baccigalupi, Giuliana Donadio, Ezio Ricca, Rachele Isticato
Summary: The development of efficient mucosal vaccines relies on appropriate vectors. The bacterial spore has been proposed as a vaccine delivery system, displaying antigens on its surface and inducing specific immune responses in animals. The advantages and drawbacks of using the spore and different approaches to display antigens are discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
F. L. Pathan, A. M. Trimukhe, R. R. Deshmukh, U. S. Annapure
Summary: Plasma processing is an effective method for controlling microorganisms in food preservation at low temperatures. The study investigated the effect of cold plasma treatment on water absorption rate and capacity of six chickpea cultivars using the Peleg model. The results showed that plasma power and treatment time had a significant impact on water absorption rate, but not on water uptake capacity.
SCIENTIFIC REPORTS
(2023)
Article
Biophysics
Michal Bodik, Daniela Krajcikova, Jakub Hagara, Eva Majkova, Imrich Barak, Peter Siffalovic
Summary: The Bacillus subtilis spore coat is a bacterial proteinaceous structure with unique characteristics such as self-organization, toughness, and flexibility. It is composed of over 80 coat proteins, some of which form two-dimensional crystal structures. By applying Grazing-Incidence Wide-Angle X-ray Scattering (GIWAXS), researchers were able to investigate the diffraction pattern of CotY 2D crystals formed on Langmuir monolayer films. The diffractions obtained pave the way for determining the 3D structure of coat proteins using strong X-ray sources.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Review
Biotechnology & Applied Microbiology
Peter Setlow, Graham Christie
Summary: Spores can spread food spoilage, diseases, and intoxications, and methods for spore killing include heat, radiation, high pressure, and chemicals. Factors to consider in choosing methods for spore killing include cost, efficacy, operation scope, and compatibility.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Microbiology
Philipp Stier, Ulrich Kulozik
Summary: This study investigates the standardization of the production method of mushroom spores and finds that the two-step manufacturing process can achieve higher spore yields.
Article
Microbiology
Benjamin Yu, Julia Kanaan, Hannah Shames, James Wicander, Makunda Aryal, Yunfeng Li, George Korza, Stanley Brul, Gertjan Kramer, Yong-qing Li, Frank C. Nichols, Bing Hao, Peter Setlow
Summary: 2Duf is a protein located in the inner membrane of spores in Bacillus species. The absence of YetF or YdfS leads to decreased resistance to wet heat and damage to spore core components. YetF can modify the inner membrane structure to increase spore resistance to wet heat.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Juan Wen, Jan P. P. M. Smelt, Norbert O. E. Vischer, Arend L. de Vos, Peter Setlow, Stanley Brul
Summary: The heat activation and heat damage profile of Bacillus subtilis spores were determined in this study. Heat activation at 40 to 70 degrees C promoted spore germination, while heat inactivation and damage to germination-related molecules occurred at 70 degrees C.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Vincenzo Zammuto, Maria Teresa Caccamo, Salvatore Magazu, Antonio Spano, Salvatore Guglielmino, Concetta Gugliandolo
Summary: Spores from Bacillus horneckiae SBP3 showed higher resistance to extreme conditions compared to their relatives B. horneckiae DSM 23495(T) and B. subtilis 168. This enhanced resistance may be attributed to the stability of SBP3 spore proteins. The study provides basic information for further comparative studies and applications in fields like astrobiology.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Giannina Espina, Paulina Caceres-Moreno, Guillermo Mejias-Navarrete, Minghua Ji, Junsong Sun, Jenny M. Blamey
Summary: Laccases are important enzymes with wide substrate specificity and biotechnological potential. This research presents a novel spore-coat laccase with high specific activity, broad temperature range, and the ability to rapidly decolorize various synthetic dyes at 40 degrees Celsius.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
A. M. DeMarco, G. Korza, M. R. Granados, W. W. K. Mok, P. Setlow
Summary: The study found that dodecylamine may be a universal sporicide for Firmicute species, killing spores by damaging their inner membrane. Additionally, spores killed by dodecylamine cannot be recovered by additions to recovery media, indicating that these spores are truly dead.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Review
Physics, Applied
Pietro Ranieri, Nicholas Sponsel, Jon Kizer, Marcela Rojas-Pierce, Ricardo Hernandez, Luciano Gatiboni, Amy Grunden, Katharina Stapelmann
Summary: Plasma agriculture focuses on the role of nonthermal plasma in enhancing plant growth, stress tolerance, and antimicrobial effects. The review discusses key developmental stages of plants, their needs, different growth environments, and the plant microbiome. This provides a foundation for designing plasma-based fertilization strategies to meet the needs of plants and their ecosystem.
PLASMA PROCESSES AND POLYMERS
(2021)
Article
Physics, Applied
B. Myers, P. Ranieri, T. Smirnova, P. Hewitt, D. Peterson, M. Herrera Quesada, E. Lenker, K. Stapelmann
Summary: Plasma-generated hydroxyl radicals and oxygen atoms were investigated, revealing that the HTA assay cannot distinguish between OH radicals and atomic oxygen, leading to inaccuracies in measuring aqueous OH densities. The experiments showed the significant role of atomic oxygen in plasma-induced liquid chemistry, highlighting the need for diagnostic techniques that take this into account.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2021)
Article
Oncology
Hager Mohamed, Eric Gebski, Rufranshell Reyes, Samuel Beane, Brian Wigdahl, Fred C. Krebs, Katharina Stapelmann, Vandana Miller
Summary: The study examined the responses of two human leukemic cell lines to NTP-generated reactive oxygen and nitrogen species, demonstrating that cells can modulate the RONS-mediated changes in liquid chemistry, and the immunomodulatory effects of NTP may be independent of its cytotoxicity.
Article
Engineering, Mechanical
Naveen Pillai, Nicholas L. Sponsel, Katharina Stapelmann, Igor A. Bolotnov
Summary: In this study, DNS coupled with interface tracking is utilized to investigate the bubble formation, departure, and ascent through water. The research reveals phenomena that are often not observed in conventional studies, such as the leaning effect and the convergence of bubble shapes.
JOURNAL OF FLUIDS ENGINEERING-TRANSACTIONS OF THE ASME
(2022)
Article
Physics, Applied
Katharina Stapelmann, Brayden Myers, Maria Herrera Quesada, Eleanor Lenker, Pietro J. Ranieri
Summary: Applied cold atmospheric plasma allows for controlled delivery of reactive oxygen and nitrogen species tailored for specific applications, providing the possibility to select species for target modification through selective chemistries. Different gas admixtures exhibit significant differences in reaction pathways and oxidation products, offering selective targeting based on the chemistry produced.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2021)
Article
Physics, Applied
Hannah L. Wapshott-Stehli, Brayden G. Myers, Maria J. Herrera Quesada, Amy Grunden, Katharina Stapelmann
Summary: Enzymes like OleT(JE) are commonly used in the industry, but the presence of hydrogen peroxide can cause overoxidation. This study explores the use of an atmospheric pressure plasma jet to generate hydrogen peroxide slowly in situ, providing a reliable reaction mediator for OleT(JE) activity.
PLASMA PROCESSES AND POLYMERS
(2022)
Review
Pharmacology & Pharmacy
Ana L. A. N. Barros, Abdelaaty Hamed, Mariela Marani, Daniel C. Moreira, Peter Eaton, Alexandra Placido, Massuo J. Kato, Jose Roberto S. A. Leite
Summary: Urodele amphibians, such as salamanders and newts, have skin secretions that contain a wide range of bioactive molecules, including antimicrobial, antioxidant, vasoactive, immune system modulation, and dermal wound healing properties. These molecules can serve as new scaffolds for drug development in the pharmaceutical industry.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Oncology
Andreanne G. Vasconcelos, Ana Luisa A. N. Barros, Wanessa F. Cabral, Daniel C. Moreira, Ingrid Gracielle M. da Silva, Amandda E. Silva-Carvalho, Miguel P. de Almeida, Lucas F. F. Albuquerque, Raimunda C. dos Santos, Ana Karolinne S. Brito, Felipe Saldanha-Araujo, Daniel D. R. Arcanjo, Maria do Carmo C. Martins, Tatiana K. dos S. Borges, Sonia N. Bao, Alexandra Placido, Peter Eaton, Selma A. S. Kuckelhaus, Jose Roberto S. A. Leite
Summary: The study developed a self-emulsifying drug delivery system (nanoLPG) loaded with lycopene, which demonstrated good biocompatibility and colloidal stability in vivo. This system successfully delivered lycopene to the liver, kidney, and prostate in mice, enhancing its cytotoxicity against prostate cancer cells.
CANCER NANOTECHNOLOGY
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Mounir Laroussi, Sander Bekeschus, Michael Keidar, Annemie Bogaerts, Alexander Fridman, Xinpei Lu, Kostya Ostrikov, Masaru Hori, Katharina Stapelmann, Vandana Miller, Stephan Reuter, Christophe Laux, Ali Mesbah, James Walsh, Chunqi Jiang, Selma Mededovic Thagard, Hiromasa Tanaka, Dawei Liu, Dayun Yan, Maksudbek Yusupov
Summary: This article provides an overview of the applications of plasma in technology and healthcare, discusses innovative concepts and approaches to overcoming current challenges, and proposes ideas and concepts to guide future research efforts.
IEEE TRANSACTIONS ON RADIATION AND PLASMA MEDICAL SCIENCES
(2022)
Article
Physics, Applied
Naveen Pillai, Nicholas L. Sponsel, J. T. Mast, Mark J. Kushner, Igor A. Bolotnov, Katharina Stapelmann
Summary: The shape of bubbles and the proximity to electrodes play critical roles in the formation and propagation of plasma in liquids. Experimental and numerical studies were conducted to investigate the mechanisms of bubble formation and plasma propagation.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2022)
Article
Materials Science, Coatings & Films
Nicholas L. Sponsel, Sophia Gershman, Maria J. Herrera J. Quesada, Jacob T. Mast, Katharina Stapelmann
Summary: The experimental investigation focused on high voltage nanosecond pulse driven electric discharges in de-ionized water with an argon bubble suspended between two electrodes. The results showed that discharge initiates in the water at the tip of the anode and rapidly extends to the apex of the bubble, accompanied by light emission. The steep rate of voltage rise and the short discharge development time suggest that cavitation may be the mechanism for discharge initiation and propagation in water.
JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A
(2022)
Review
Physics, Fluids & Plasmas
Luis L. Alves, Markus M. Becker, Jan van Dijk, Timo Gans, David B. Go, Katharina Stapelmann, Jonathan Tennyson, Miles M. Turner, Mark J. Kushner
Summary: The field of low-temperature plasmas (LTPs) excels by its intellectual diversity, interdisciplinarity, and range of applications. Communication challenges arise due to diverse reporting practices and expectations. This paper addresses these challenges by discussing standards for measurements, diagnostics, computations, reporting, and plasma sources to improve communication and transparency within the LTP field.
PLASMA SOURCES SCIENCE & TECHNOLOGY
(2023)
Article
Physics, Applied
Jordyn Polito, Maria J. Herrera Quesada, Katharina Stapelmann, Mark J. Kushner
Summary: A reaction mechanism for the oxidation of cysteine in CAP activated water was developed and validated through experimental data. The results showed that operating parameters can be adjusted to selectively produce desired cysteine oxidation products, including nitrosylated products.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2023)
Article
Physics, Applied
Duncan Trosan, Patrick Walther, Stephen Mclaughlin, Deepti Salvi, Aaron Mazzeo, Katharina Stapelmann
Summary: This paper investigates the impact of different repeated lattice structures on the geometric properties of SDBD. The results show that the average electric field is not significantly affected by applied voltage but exhibits minor variations due to the geometry of the electrode lattice structures. Additionally, the plasma power does not follow a linear relationship with the perimeter in these more complicated lattice structures.
PLASMA PROCESSES AND POLYMERS
(2023)
Article
Physics, Fluids & Plasmas
Joshua Morsell, Duncan Trosan, Katharina Stapelmann, Steven Shannon
Summary: The study of plasma surface ionization waves (SIWs) has mainly focused on planar surfaces and periodic structures in recent years. However, in practical applications, substrates often have non-planar morphology and targets may have heterogeneous composition, which fall under the category of complex interfaces. This study investigates plasma SIWs incident on a complex target consisting of a single channel cut into glass slides. The velocities of the SIWs in the channel portion and the radially propagating portion were tracked. The results show that the surface wave velocities are primarily affected by pulse energy rather than channel geometry. The study also discusses the morphology of surface waves and their impact on applications, and introduces a simple model to understand the mechanisms behind SIW escape from channels.
PLASMA SOURCES SCIENCE & TECHNOLOGY
(2023)