Recent advances in microfluidic single-cell analysis and its applications in drug development

Signatures of malignant cells and novel therapeutic targets revealed by single‐cell sequencing in lung adenocarcinoma

(2022) Jiaqi Liang et al.   Cancer Medicine

Negative Magnetophoresis Focusing Microchips Online-Coupled with ICP–MS for High-Throughput Single-Cell Analysis

(2022) Zhenna Chen et al.   ANALYTICAL CHEMISTRY

In-situ monitoring calcium signaling through tumor microtubes for single cell-cell communication via an open microfluidic probe

(2022) Shuo Feng et al.   BIOSENSORS & BIOELECTRONICS

Whole Genome Amplification in Preimplantation Genetic Testing in the Era of Massively Parallel Sequencing

(2022) Ludmila Volozonoka et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes

(2022) Jina Ko et al.   NATURE BIOTECHNOLOGY

D2O-Probed Raman Microspectroscopy Distinguishes the Metabolic Dynamics of Macromolecules in Organellar Anticancer Drug Response

(2021) Maryam Hekmatara et al.   ANALYTICAL CHEMISTRY

In Situ Stable Generation of Reactive Intermediates by Open Microfluidic Probe for Subcellular Free Radical Attack and Membrane Labeling

(2021) Qiang Zhang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Single-cell lineages reveal the rates, routes, and drivers of metastasis in cancer xenografts

(2021) Jeffrey J. Quinn et al.   SCIENCE

Inhibitory CD161 receptor identified in glioma-infiltrating T cells by single-cell analysis

(2021) Nathan D. Mathewson et al.   CELL

Clonal evolution in liver cancer at single-cell and single-variant resolution

(2021) Xianbin Su et al.   Journal of Hematology & Oncology

Spatially Resolved Mass Spectrometry at the Single Cell: Recent Innovations in Proteomics and Metabolomics

(2021) Michael J. Taylor et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Multimodal pooled Perturb-CITE-seq screens in patient models define mechanisms of cancer immune evasion

(2021) Chris J. Frangieh et al.   NATURE GENETICS

Joint profiling of DNA and proteins in single cells to dissect genotype-phenotype associations in leukemia

(2021) Benjamin Demaree et al.   Nature Communications

Metabolism-Based Capture and Analysis of Circulating Tumor Cells in an Open Space

(2021) Weifei Zhang et al.   ANALYTICAL CHEMISTRY

Applications of Single-Cell DNA Sequencing

(2021) Gilad D. Evrony et al.   Annual Review of Genomics and Human Genetics

In Situ Single Cell Proteomics Reveals Circulating Tumor Cell Heterogeneity during Treatment

(2021) K. Kamil Reza et al.   ACS Nano

A microfluidic array device for single cell capture and intracellular Ca2+ response analysis induced by dynamic biochemical stimulus

(2021) Wenbo Wei et al.   BIOSCIENCE REPORTS

Single cell DNA sequencing reveals punctuated and gradual clonal evolution in hepatocellular carcinoma

(2021) Lin Guo et al.   GASTROENTEROLOGY

Chromatin Mechanisms Driving Cancer

(2021) Berkley Gryder et al.   Cold Spring Harbor Perspectives in Biology

Single-Cell Digital Microfluidic Mass Spectrometry Platform for Efficient and Multiplex Genotyping of Circulating Tumor Cells

(2021) Qingyu Ruan et al.   ANALYTICAL CHEMISTRY

Development of droplet microfluidics capable of quantitative estimation of single-cell multiplex proteins

(2021) Hongyu Yang et al.   JOURNAL OF MICROMECHANICS AND MICROENGINEERING

Active single cell encapsulation using SAW overcoming the limitations of Poisson distribution

(2021) Andreas Link et al.   LAB ON A CHIP

Multiphysics microfluidics for cell manipulation and separation: a review

(2021) Haotian Cha et al.   LAB ON A CHIP

Identifying phenotype-associated subpopulations by integrating bulk and single-cell sequencing data

(2021) Duanchen Sun et al.   NATURE BIOTECHNOLOGY

Microfluidics-based single cell sequencing: moving towards more physiological analysis

(2021) Shuo Feng et al.   Science China-Chemistry

Recent advances in single-cell analysis: Encapsulation materials, analysis methods and integrative platform for microfluidic technology

(2021) Jiadi Sun et al.   TALANTA

Microfluidics applications for high-throughput single cell sequencing

(2021) Wen-min Zhou et al.   JOURNAL OF NANOBIOTECHNOLOGY

Targeted therapy guided by single-cell transcriptomic analysis in drug-induced hypersensitivity syndrome: a case report

(2020) Doyoung Kim et al.   NATURE MEDICINE

Microfluidic Probe for In-Situ Extraction of Adherent Cancer Cells to Detect Heterogeneity Difference by Electrospray Ionization Mass Spectrometry

(2020) Po Liu et al.   ANALYTICAL CHEMISTRY

Single-Cell Analyses Inform Mechanisms of Myeloid-Targeted Therapies in Colon Cancer

(2020) Lei Zhang et al.   CELL

Affinity-Bead Assisted Mass Spectrometry (Affi-BAMS): A Multiplexed Microarray Platform for Targeted Proteomics

(2020) Ghaith M. Hamza et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

High-throughput single-cell activity-based screening and sequencing of antibodies using droplet microfluidics

(2020) Annabelle Gérard et al.   NATURE BIOTECHNOLOGY

Microfluidic adhesion analysis of single glioma cells for evaluating the effect of drugs

(2020) Qiang Zhang et al.   Science China-Chemistry

Microfluidics in Single-Cell Virology: Technologies and Applications

(2020) Wu Liu et al.   TRENDS IN BIOTECHNOLOGY

Conversion Efficiencies of a Few Living Microbial Cells Detected at a High Throughput by Droplet-Based ESI-MS

(2020) Martin Schirmer et al.   ANALYTICAL CHEMISTRY

Stromal cell diversity associated with immune evasion in human triple‐negative breast cancer

(2020) Sunny Z Wu et al.   EMBO JOURNAL

Recent advances of single-cell RNA sequencing technology in mesenchymal stem cell research

(2020) Guan Zheng et al.   World Journal of Stem Cells

Integrating genetic and non-genetic determinants of cancer evolution by single-cell multi-omics

(2020) Anna S. Nam et al.   NATURE REVIEWS GENETICS

The epigenetic basis of cellular heterogeneity

(2020) Benjamin Carter et al.   NATURE REVIEWS GENETICS

Single-Cell Techniques and Deep Learning in Predicting Drug Response

(2020) Zhenyu Wu et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Single-cell metabolomics by mass spectrometry: Advances, challenges, and future applications

(2019) Ahmed Ali et al.   TRAC-TRENDS IN ANALYTICAL CHEMISTRY

Single-cell chromatin immunocleavage sequencing (scChIC-seq) to profile histone modification

(2019) Wai Lim Ku et al.   NATURE METHODS

Unravelling Intratumoral Heterogeneity through High-Sensitivity Single-Cell Mutational Analysis and Parallel RNA Sequencing

(2019) Alba Rodriguez-Meira et al.   MOLECULAR CELL

Phosphate Assay Kit in One Cell for Electrochemical Detection of Intracellular Phosphate Ions at Single Cells

(2019) Haiyan Xu et al.   Frontiers in Chemistry

An Integrative Model of Cellular States, Plasticity, and Genetics for Glioblastoma

(2019) Cyril Neftel et al.   CELL

The significance of gene mutations across eight major cancer types

(2019) Prosenjit Paul et al.   MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH

Subpopulation Detection and Their Comparative Analysis across Single-Cell Experiments with scPopCorn

(2019) Yijie Wang et al.   Cell Systems

Quantitative Detection and Real-Time Monitoring of Endogenous mRNA at the Single Live Cell Level Using a Ratiometric Molecular Beacon

(2019) Xufei Feng et al.   ACS Applied Materials & Interfaces

Single-Cell Phenotypic Profiling of CTCs in Whole Blood Using an Integrated Microfluidic Device

(2019) Haimeng Pei et al.   ANALYTICAL CHEMISTRY

Massively parallel single-cell chromatin landscapes of human immune cell development and intratumoral T cell exhaustion

(2019) Ansuman T. Satpathy et al.   NATURE BIOTECHNOLOGY

Single-cell RNA sequencing reveals compartmental remodeling of tumor-infiltrating immune cells induced by anti-CD47 targeting in pancreatic cancer

(2019) Yu Pan et al.   Journal of Hematology & Oncology

Single-cell analysis for proteome and related researches

(2019) Xi Shao et al.   TRAC-TRENDS IN ANALYTICAL CHEMISTRY

More than efficacy revealed by single-cell analysis of antiviral therapeutics

(2019) Wu Liu et al.   Science Advances

Immune profiling of human tumors identifies CD73 as a combinatorial target in glioblastoma

(2019) Sangeeta Goswami et al.   NATURE MEDICINE

The application of single-cell sequencing technology in the diagnosis and treatment of hepatocellular carcinoma

(2019) Kai Kang et al.   Annals of Translational Medicine

Vacuum ultraviolet laser desorption/ionization mass spectrometry imaging of single cells with submicron craters

(2018) Jia Wang et al.   ANALYTICAL CHEMISTRY

Moderate UV Exposure Enhances Learning and Memory by Promoting a Novel Glutamate Biosynthetic Pathway in the Brain

(2018) Hongying Zhu et al.   CELL

Chemoresistance Evolution in Triple-Negative Breast Cancer Delineated by Single-Cell Sequencing

(2018) Charissa Kim et al.   CELL

Multiclonal Invasion in Breast Tumors Identified by Topographic Single Cell Sequencing

(2018) Anna K. Casasent et al.   CELL

Machine Learning helps Identify New Drug Mechanisms in Triple-Negative Breast Cancer

(2018) Arjun P. Athreya et al.   IEEE TRANSACTIONS ON NANOBIOSCIENCE

Single cell epigenome sequencing technologies

(2018) Lu Wen et al.   MOLECULAR ASPECTS OF MEDICINE

Transcript-indexed ATAC-seq for precision immune profiling

(2018) Ansuman T. Satpathy et al.   NATURE MEDICINE

Stem cells: lineage tracing lets single cells talk about their past

(2018) Vivien Marx   NATURE METHODS

Single-cell transcriptional profiling: a window into embryonic cell-type specification

(2018) Blanca Pijuan-Sala et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Recent advances in single cell manipulation and biochemical analysis on microfluidics

(2018) Dan Gao et al.   ANALYST

Single-cell RNA-seq analysis identifies markers of resistance to targeted BRAF inhibitors in melanoma cell populations

(2018) Yu-Jui Ho et al.   GENOME RESEARCH

Unravelling subclonal heterogeneity and aggressive disease states in TNBC through single-cell RNA-seq

(2018) Mihriban Karaayvaz et al.   Nature Communications

Live single cell mass spectrometry reveals cancer‐specific metabolic profiles of circulating tumor cells

(2018) Yasmine Abouleila et al.   CANCER SCIENCE

Integrative single-cell analysis of transcriptome, DNA methylome and chromatin accessibility in mouse oocytes

(2018) Chan Gu et al.   CELL RESEARCH

Small-Molecule Fluorescent Probes for Live-Cell Super-Resolution Microscopy

(2018) Lu Wang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Comparative Analysis of Droplet-Based Ultra-High-Throughput Single-Cell RNA-Seq Systems

(2018) Xiannian Zhang et al.   MOLECULAR CELL

Rare cell variability and drug-induced reprogramming as a mode of cancer drug resistance

(2017) Sydney M. Shaffer et al.   NATURE

Scaling single-cell genomics from phenomenology to mechanism

(2017) Amos Tanay et al.   NATURE

Single-cell genome sequencing at ultra-high-throughput with microfluidic droplet barcoding

(2017) Freeman Lan et al.   NATURE BIOTECHNOLOGY

Scaling by shrinking: empowering single-cell 'omics' with microfluidic devices

(2017) Sanjay M. Prakadan et al.   NATURE REVIEWS GENETICS

Single-neuron identification of chemical constituents, physiological changes, and metabolism using mass spectrometry

(2017) Hongying Zhu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The cancer epigenome: Concepts, challenges, and therapeutic opportunities

(2017) Mark A. Dawson   SCIENCE

Single-cell transcriptomics to explore the immune system in health and disease

(2017) Michael J. T. Stubbington et al.   SCIENCE

A phenotypically and functionally distinct human T H 2 cell subpopulation is associated with allergic disorders

(2017) Erik Wambre et al.   Science Translational Medicine

Nanogrid single-nucleus RNA sequencing reveals phenotypic diversity in breast cancer

(2017) Ruli Gao et al.   Nature Communications

Massively parallel digital transcriptional profiling of single cells

(2017) Grace X. Y. Zheng et al.   Nature Communications

Fast Single-Cell Patterning for Study of Drug-Induced Phenotypic Alterations of HeLa Cells Using Time-of-Flight Secondary Ion Mass Spectrometry

(2016) Lu Huang et al.   ANALYTICAL CHEMISTRY

Microfluidic techniques for high throughput single cell analysis

(2016) Amy Reece et al.   CURRENT OPINION IN BIOTECHNOLOGY

On-chip analysis, indexing and screening for chemical producing bacteria in a microfluidic static droplet array

(2016) Sungho Jang et al.   LAB ON A CHIP

Single-cell barcoding and sequencing using droplet microfluidics

(2016) Rapolas Zilionis et al.   Nature Protocols

Nanokit for single-cell electrochemical analyses

(2016) Rongrong Pan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Antibiotic failure mediated by a resistant subpopulation in Enterobacter cloacae

(2016) Victor I. Band et al.   Nature Microbiology

Design and Analysis of Single-Cell Sequencing Experiments

(2015) Dominic Grün et al.   CELL

Droplet Barcoding for Single-Cell Transcriptomics Applied to Embryonic Stem Cells

(2015) Allon M. Klein et al.   CELL

Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets

(2015) Evan Z. Macosko et al.   CELL

Translational Implications of Tumor Heterogeneity

(2015) M. Jamal-Hanjani et al.   CLINICAL CANCER RESEARCH

The Technology and Biology of Single-Cell RNA Sequencing

(2015) Aleksandra A. Kolodziejczyk et al.   MOLECULAR CELL

Insulator dysfunction and oncogene activation in IDH mutant gliomas

(2015) William A. Flavahan et al.   NATURE

Single-cell analysis tools for drug discovery and development

(2015) James R. Heath et al.   NATURE REVIEWS DRUG DISCOVERY

Single-cell RNA-seq reveals dynamic paracrine control of cellular variation

(2014) Alex K. Shalek et al.   NATURE

Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity

(2014) Sébastien A Smallwood et al.   NATURE METHODS

Block-Cell-Printing for live single-cell printing

(2014) K. Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mass spectrometry-based metabolomics of single yeast cells

(2013) A. J. Ibanez et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cellular Heterogeneity: Do Differences Make a Difference?

(2010) Steven J. Altschuler et al.   CELL