Fish tissue conversion factors for mercury, cadmium, lead and nine per- and polyfluoroalkyl substances for use within contaminant monitoring

Fish tissue levels have to comply with environmental quality standards (EQSs) within the European Water Framework Directive. However, within monitoring, contaminants are sometimes measured in a different tissue than the tissue for which the environmental (whole fish) or human (fillet (equivalent to muscle tissue)) quality standard is set. Tissue con-version factors (k), describing the relationship between concentrations in different tissues, can be used to obtain a qual-ity standard for the appropriate tissue. Several different approaches have been suggested for the calculation of k. For monitoring purposes, we propose the use of a simple, easy reproducible approach that assumes proportionality be-tween two tissue, or tissue and whole fish, concentrations. This allows for an easy comparison of studies and adoption of ks into independent monitoring programs. Here, we determined ks for three metals (mercury (Hg), lead (Pb), cad-mium (Cd)) and nine per-and polyfluoroalkyl substances (PFAS) including perfluorooctanesulfonic acid (PFOS) across six marine and freshwater fish species from Northern European lakes and the Baltic Sea. We found significant species differences for Hg for kmuscle/whole fish, for Cd and Pb for kliver/whole fish and for Cd for kliver/muscle. For perfluoroalkyl carboxylic acids (PFCA), we found a chain length dependence with lowest kliver/muscle at low and high chain lengths (C8, C13) and highest for median chain lengths (C9-C12). Further, there were differences between fish species with kliver/musclefor PFOS almost doubling from eelpout (10.3) to herring (19.2) and increasing up to a factor 4 between eelpout and herring for other PFASs. FOSA had two distinctive groups, herring with a kliver/muscle of 48.7 and a second group with ks of 2.3 to 5.9 for all other fish species. Our results suggest that differences in the tissue somatic index, and contaminant uptake, tissue transfer and metabolism result in the need for species-specific ks within monitoring.

Automated summary

Fish tissue levels must meet environmental quality standards (EQSs) set by the European Water Framework Directive. However, contaminants are sometimes measured in different tissues than the ones that the standards are set for. Tissue conversion factors (k) can be used to obtain quality standards for the appropriate tissues. We propose a simple and reproducible approach that assumes proportionality between two tissues or tissue and whole fish concentrations for monitoring purposes.