Article
Biochemical Research Methods
Sampson D. Umoh, Gomotsang Bojase, Ishmael B. Masesane, Runner T. Majinda, Kwenga F. Sichilongo
Summary: This study identified a total of 97 phytoconstituents, including 17 bioactive compounds, in Combretum platypetalum for the first time. The results suggest that the extraction methods used can influence the concentration of compounds. These findings support the potential applications of C. platypetalum in drug discovery.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Emmanuel Orman, Samuel Oppong Bekoe, Samuel Asare-Nkansah, Ina Kralisch, Jonathan Jato, Verena Spiegler, Christian Agyare, Emelia Oppong Bekoe, Andreas Hensel
Summary: This study developed quality control methods for herbal materials, focusing on Phyllanthus urinaria (PU), a widely used medicinal plant in Ghana. The study established identification methods for PU samples and proposed acceptance criteria for active markers. The study also found pesticide contamination in the PU samples. The analytical protocols and insights provided by this study are important for considering quality standards for PU in African pharmacopoeias.
Article
Environmental Sciences
Ivana Grgic, Katarina A. Cetinic, Zrinka Karacic, Ana Previsic, Marko Rozman
Summary: This study conducted an experiment to assess the effects of microplastics and pharmaceuticals on a simplified freshwater ecosystem. It found that moss can act as a sink for microplastics and that caddisflies ingest microplastics when feeding on moss, causing stress. Additionally, the study discovered similarities in the effects of microplastics and pharmaceuticals on aquatic organisms.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Rosa Bonaventura, Francesca Zito, Lorenzo Morroni, David Pellegrini, Francesco Regoli, Annalisa Pinsino
Summary: The management of dredged materials disposal is regulated by environmental normative requirements and supported by the integration of chemical data with ecotoxicological characterization. The reliability of a bioassay to assess toxicity of dredged sediments relies on the selection of quality criteria and the use of sea urchin embryo-toxicity bioassay is essential for evaluating sediment quality in harbour areas. The development and validation of Integrative Toxicity Indexes, particularly ITI 4.0, improves the causative relationship between sediment quality and sea urchin embryonic development.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2021)
Article
Biochemical Research Methods
Stephen A. Wise
Summary: Certified reference materials (CRMs) are important tools for validating analytical methods and demonstrating quality, but their use is often overlooked in analytical chemistry journals. This article explores the potential benefits of using CRMs, even without certified values.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Y. Lan Pham, Ruyi Yu, Jonathan Beauchamp
Summary: The Peppermint Experiment aims to address the lack of comparability in breath biomarker studies by analyzing the washout profiles of peppermint oil constituents in exhaled breath. The experiment involves measuring samples from a group of volunteers using three different analytical platforms. The results show variable washout profiles within and between individuals, indicating differences in absorption, distribution, and metabolism of the constituents. However, the low variability between instruments suggests that discrepancies in benchmark values reported in the literature are mostly due to biological variability rather than method or platform performance.
JOURNAL OF BREATH RESEARCH
(2023)
Article
Pharmacology & Pharmacy
E. Sun, Ran Huang, Ke Ding, Ling Wang, Jian Hou, Xiaobin Tan, Yingjie Wei, Liang Feng, Xiaobin Jia
Summary: This study used a strategy based on comprehensive GC-TOF/MS metabolomics and network pharmacology analysis to identify the active compounds, targets, and metabolic pathways involved in the enhanced kidney yang deficiency effects of fried Epimedium. The findings indicate that fried Epimedium can improve kidney yang deficiency by regulating oxidative stress and amino acid metabolism.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Food Science & Technology
Alejandro Garcia-Juan, Nuria Leon, Sergio Armenta, Olga Pardo
Summary: This study successfully developed and validated an analytical method for the simultaneous determination of ergot, tropane, and pyrrolizidine alkaloids in cereal-based food. The method showed high recoveries and precision, meeting the validation requirements. Analysis of reference materials further confirmed the reliability of the method. Analysis of actual samples revealed the presence of ergot alkaloids and pyrrolizidine alkaloids in some samples.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Multidisciplinary
Talia Marie Sebastian, Ke Qu, Xiangqun Zeng
Summary: The revamped laboratory experiment provides students with the opportunity to develop a multidimensional pH sensor using conducting polyaniline material, compare its performance against traditional methods like glass pH electrodes, and learn about the electrochromic properties of polyaniline. This hands-on approach reinforces fundamental concepts in analytical chemistry and encourages creativity in developing next-generation analytical sensor technology.
JOURNAL OF CHEMICAL EDUCATION
(2021)
Article
Biochemical Research Methods
Paramita Saha, Murali Monohar Pandey
Summary: The present study developed and validated a simple RP-HPLC method for estimation of EFZ using DoE approach. Critical method variables were screened using Plackett-Burman design and optimized with Box-Behnken design. The method was capable of successfully estimating EFZ in marketed formulation.
JOURNAL OF CHROMATOGRAPHIC SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Santos Alves Vicentini Neto, Lisia Maria Gobbo dos Santos, Cristiane Barata Silva, Mayssa Andrade Fonseca, Carolina Duque Magalhaes, Silvana do Couto Jacob
Summary: The disorderly growth of cities has led to environmental pollution, particularly in the emission and deposition of metals, which pose a risk to human health. Currently, there is a lack of a population biomonitoring program, which could be instrumental in guiding preventive measures and promoting collective health. This study successfully optimized and validated an analytical method for quantifying As, Cd, Pb, and Hg in blood samples.
REVISTA VIRTUAL DE QUIMICA
(2022)
Article
Pharmacology & Pharmacy
Mary-Ann Archer, Doris Kumadoh, Samuel Nii-Bortier Gaizer, Adelaide Mensah, Jonathan Jato, Micheal Odoi Kyene, Susana Oteng Mintah, Genevieve Naana Yeboah, Paul Kwesi Sodzi, Ofosua Adi-Dako
Summary: This study successfully transformed Antiaris into oral capsules to overcome the challenges of its bulkiness and bitterness. The formulated capsules showed good flow properties, dissolution efficiency, and similarity to the Antiaris decoction. They can be considered as viable substitutes for Antiaris.
ADVANCES IN PHARMACOLOGICAL AND PHARMACEUTICAL SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Benjamin Kingsley Harley, David Neglo, Mike Okweesi Aggrey, Anthony Martin Quagraine, Emmanuel Orman, Jonathan Jato, Nana Ama Mireku-Gyimah, Cedric Dzidzor K. Amengor, Theophilus Christian Fleischer
Summary: This study evaluated the antifungal activities of Sclerocarya birrea leaf and stem bark extracts against Candida albicans, showing significant inhibitory and fungicidal effects. The extracts also demonstrated inhibition of biofilm formation and mature biofilms. Metabolite profiling identified the presence of polyphenolic compounds, with flavonoids abundant in the leaves and condensed tannins abundant in the stem bark. The study highlights the potential of S. birrea extracts as antifungal agents.
BIOMED RESEARCH INTERNATIONAL
(2022)
Article
Chemistry, Multidisciplinary
Cedric D. K. Amengor, Abena Brobbey, Isaac Ayensu, Emmanuel Orman, Paul Peprah, Billy Mawunyo Abudey, Seth Ayihi, Claudia Naa Dedei Attoh, Alberta Ade, Paul Kwesi Sodzi, James Sarpong Aboagye
Summary: Two new acid-base indicators, IND-X and IND-Y, were successfully synthesized and validated. The experimental results showed that IND-X and IND-Y exhibited similar behavior to commonly used indicators in acid-base titrations, and they were effective in pharmaceutical applications. Therefore, IND-X and IND-Y are proposed as alternative indicators for routine acid-base titrations and pharmaceutical analysis.
JOURNAL OF CHEMISTRY
(2022)
Review
Integrative & Complementary Medicine
Jonathan Jato, Emmanuel Orman, Yaw Duah Boakye, Emelia Oppong Bekoe, Samuel Oppong Bekoe, Samuel Asare-Nkansah, Verena Spiegler, Andreas Hensel, Eva Liebau, Christian Agyare
Summary: Soil-transmitted helminthiasis is a sanitary problem in Africa, causing economic burden and poor animal health. Medicinal plants in Africa have shown potential as anthelmintic agents. This review examines studies conducted on African medicinal plants in the past two decades and highlights their significant anthelmintic potential.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2022)
Article
Plant Sciences
Joshua Jacobtorweihen, Verena Spiegler
Summary: Marine algae are a rich source of halogenated compounds, particularly the bioactive brominated phenols. Isolation of these natural products is essential for biological activity testing as they are not typically commercially available. However, identifying desired compounds in crude extracts or fractions via high-resolution LC-MS can be challenging. This review provides an overview of the distribution and chemotaxonomic relevance of bromophenols in marine algae, along with key analytical features for their mass spectrometric identification. Additionally, a comprehensive database listing brominated phenols from marine algae and their key analytical properties has been constructed.
PHYTOCHEMISTRY REVIEWS
(2023)
Article
Infectious Diseases
Cornelius C. Dodoo, Hayford Odoi, Adelaide Mensah, Karikari Asafo-Adjei, Ruth Ampomah, Lydia Obeng, Jonathan Jato, Araba Hutton-Nyameaye, Thelma A. Aku, Samuel O. Somuah, Emmanuel Sarkodie, Emmanuel Orman, Kwadwo A. Mfoafo, Inemesit O. Ben, Eneyi E. Kpokiri, Fatima Abba, Yogini H. Jani
Summary: This study aimed to develop a local antibiogram for the Ho Teaching Hospital by collecting and analyzing data on bacterial isolates and their antibiotic resistance patterns. The results showed that the majority of the isolates exhibited high resistance to the antibiotics used in the study.
JAC-ANTIMICROBIAL RESISTANCE
(2023)
Article
Biochemistry & Molecular Biology
Emmanuel Orman, Samuel Oppong Bekoe, Samuel Asare-Nkansah, Ina Kralisch, Jonathan Jato, Verena Spiegler, Christian Agyare, Emelia Oppong Bekoe, Andreas Hensel
Summary: This study developed quality control methods for herbal materials, focusing on Phyllanthus urinaria (PU), a widely used medicinal plant in Ghana. The study established identification methods for PU samples and proposed acceptance criteria for active markers. The study also found pesticide contamination in the PU samples. The analytical protocols and insights provided by this study are important for considering quality standards for PU in African pharmacopoeias.
Article
Plant Sciences
Jonathan Jato, Patrick Waindok, Francois Ngnodandi Belga Francois Ngnodandi, Emmanuel Orman, Christian Agyare, Emelia Oppong Bekoe, Christina Strube, Andreas Hensel, Eva Liebau, Verena Spiegler
Summary: The aerial parts of Phyllanthus urinaria are used in traditional medicine in West Africa against helminthiasis. A hydroacetonic extract (AWE) and ellagitannins from P. urinaria were tested in vitro against different parasites. AWE showed concentration-dependent lethal effects against C. elegans and inhibited larval migration of all animal parasites tested.