Journal
PHYTOCHEMISTRY
Volume 204, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2022.113428
Keywords
Artemisia vulgaris L; Asteraceae; Sesquiterpenoids; Triterpenoids; Anti-inflammatory effect
Categories
Funding
- Guangdong Province Modern Agricultural Industrial Technology System Innovation Team Project [2021KJ142]
- Guangzhou key research and development plan [202206010008]
- National Natural Science Foundation of China [82073977]
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In this study, several undescribed and known terpenoids were isolated from Artemisia vulgaris L. leaves, and their structures were determined using spectroscopy methods. Artemvulactone E exhibited significant anti-inflammatory effect and reduced LPS-induced COX-2 protein expression.
In this study, eight undescribed sesquiterpenoids (artemvulactone A-G and artemvulemdiol A), and two undescribed triterpenoids, (3S)-dammar-20,25-diene-3-hydroxy-24-one and (3S,23E)-dammar-20,23-diene-25-methoxy-3-ol were isolated from the leaves of Artemisia vulgaris L., together with ten known sesquiterpenoids and three known triterpenoids. The structures of these undescribed terpenoids were determined by extensive spectroscopy methods, including 1D and 2D-NMR, HRESIMS, IR, UV, X-ray diffraction, and ECD. The absolute configurations of artemvulactone A, artemvulactone D, and artemvulactone E were determined by X-ray diffraction (Cu K alpha). All isolates were evaluated for their anti-inflammatory efficacy by detecting the expression of inflammatory mediator NO in LPS-induced RAW264.7 cells, and the results indicated that artemvulactone E exhibited significant anti-inflammatory effect with an IC50 value of 0.9 +/- 0.2 mu M. Furthermore, artemvulactone E could reduce LPS-induced COX-2 protein expression dose-dependently by western blotting experiments.
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