☆ 4.6 Article

Evidence of DMSO-Induced Protein Aggregation in Cells

JOURNAL OF PHYSICAL CHEMISTRY A (2016)

Journal

JOURNAL OF PHYSICAL CHEMISTRY A

Volume 120, Issue 27, Pages 5065-5070

Publisher

AMER CHEMICAL SOC

DOI: 10.1021/acs.jpca.6b00178

Keywords

-

Categories

Chemistry, Physical Physics, Atomic, Molecular & Chemical

Funding

ESF Competitiveness and Employment objective Umbrian Regional Operational Programme (ROP)
Avviso pubblico aiuti individuali per la realizzazione di progetti di ricerca
MIUR-PRIN [2012J8 x 57P]
PAT (Autonomous Province of Trento) Grandi Progetti Project MaDEleNA
Centro Nazionale Trapianti project Crioconservazione di cellule per use clinico umano, con particolare riferimento ad alcune tipologie come le cellule staminali emopoietiche, mesenchimali e cellule riproduttive

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Abstract

We report on a study of protein aggregation induced on different cell samples by dimethyl sulfoxide (DMSO) addition. DMSO is the most commonly used cryoprotectant because it is supposed to readily diffuse across lipid bilayers, thus reducing water activity within cells; despite its large use, the mechanism of penetration and even the main interaction features with cell components are far from being understood. In the present work, infrared absorption spectroscopy is successfully applied to real time detection of chemical and structural changes occurring in cells during dehydration from water and water/DMSO suspensions. As a most interesting result, DMSO is observed to favor protein aggregation both in cellular model systems, as cultured lymphocytes and fibroblasts, and in human samples for clinic use, as hematopoietic stem cells from cord blood. This effect is evidenced at low water content, analogously to what is observed for protein solutions. Such tendency is not specific of the type of protein and suggests one possible origin of DMSO toxicity.

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Tso, S. Tsuchida, L. Tsukada, T. Tsutsui, K. Turbang, M. Turconi, C. Turski, D. Tuyenbayev, H. Ubach, A. S. Ubhi, N. Uchikata, T. Uchiyama, R. P. Udall, T. Uehara, K. Ueno, C. S. Unnikrishnan, T. Ushiba, A. Utina, H. Vahlbruch, N. Vaidya, G. Vajente, A. Vajpeyi, G. Valdes, M. Valentini, S. Vallero, V. Valsan, N. van Bakel, M. van Beuzekom, M. van Dael, J. F. J. Van den Brand, C. Van den Broeck, D. C. Vander-Hyde, M. Van der Sluys, A. Van de Walle, J. Van Dongen, H. van Haevermaet, J. V. van Heijningen, J. Vanosky, M. H. P. M. van Putten, Z. van Ranst, N. van Remortel, M. Vardaro, A. F. Vargas, V. Varma, M. Vasuth, A. Vecchio, G. Vedovato, J. Veitch, P. J. Veitch, J. Venneberg, G. Venugopalan, P. Verdier, D. Verkindt, P. Verma, Y. Verma, S. M. Vermeulen, D. Veske, F. Vetrano, A. Vicere, S. Vidyant, A. D. Viets, A. Vijaykumar, V. Villa-Ortega, M. Vina, E. T. Vincent, J. -Y. Vinet, S. Viret, A. Virtuoso, S. Vitale, H. Vocca, D. Voigt, E. R. G. von Reis, J. S. A. von Wrangel, C. Vorvick, S. P. Vyatchanin, L. E. Wade, M. Wade, K. J. Wagner, R. C. Walet, M. Walker, G. S. Wallace, L. Wallace, H. Wang, J. Z. Wang, W. H. Wang, R. L. Ward, J. Warner, M. Was, T. Washimi, N. Y. Washington, K. Watada, D. Watarai, J. Watchi, K. E. Wayt, B. Weaver, C. R. Weaving, S. A. Webster, M. Weinert, A. J. Weinstein, R. Weiss, C. M. Weller, R. A. Weller, F. Wellmann, L. Wen, P. Wessels, K. Wette, J. T. Whelan, D. D. White, B. F. Whiting, C. Whittle, O. S. Wilk, D. Wilken, K. Willetts, D. Williams, M. J. Williams, A. R. Williamson, J. L. Willis, B. Willke, C. C. Wipf, G. Woan, J. Woehler, J. K. Wofford, D. Wong, H. T. Wong, I. C. F. Wong, M. Wright, C. Wu, D. S. Wu, H. Wu, D. M. Wysocki, L. Xiao, V. A. Xu, N. Yadav, T. Yamada, H. Yamamoto, K. Yamamoto, M. Yamamoto, T. Yamamoto, T. S. Yamamoto, K. Yamashita, R. Yamazaki, F. W. Yang, K. Z. Yang, Y. -C. Yang, M. J. Yap, D. W. Yeeles, A. B. Yelikar, T. Y. Yeung, J. Yokoyama, T. Yokozawa, J. Yoo, Hang Yu, Haocun Yu, H. Yuzurihara, A. Zadrozny, A. J. Zannelli, M. Zanolin, M. Zeeshan, S. Zeidler, T. Zelenova, J. -P. Zendri, M. Zevin, J. Zhang, L. Zhang, R. Zhang, T. Zhang, Y. Zhang, C. Zhao, Yue Zhao, Yuhang Zhao, Y. Zheng, H. Zhong, R. Zhou, X. J. Zhu, Z. -H. Zhu, A. B. Zimmerman, M. E. Zucker, J. Zweizig

Summary: The global network of gravitational-wave observatories has expanded to include five detectors, providing ample data and science products for analysis and research.

ASTROPHYSICAL JOURNAL SUPPLEMENT SERIES (2023)

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Article Biophysics

Effect of plasmonic excitation on mature insulin amyloid fibrils

Elena Cambiotti, Zuzana Bednarikova, Zuzana Gazova, Paola Sassi, Eva Bystrenova, Loredana Latterini

Summary: In this study, gold nanoparticles were used as photothermal agents to alter the morphology of insulin fibrils. The effects on the structure of mature insulin fibrils upon plasmonic excitation of nanoparticles were monitored. The results indicate that amyloid aggregates can be effectively destroyed by irradiation of plasmonic nanoparticles, suggesting potential strategies for modifying the structure of amyloid fibrils.

COLLOIDS AND SURFACES B-BIOINTERFACES (2023)

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Article Biochemical Research Methods

Extracorporeal membrane oxygenation and effects on tendon tissue: A vibrational spectroscopy study

Sara Stefani, Marco Govoni, Niki Tombolesi, Leonardo Vivarelli, Dante Dallari, Marco Paolantoni, Paola Sassi, Assunta Morresi

Summary: In this study, Raman microspectroscopy and attenuated total reflection-Fourier transform infrared micro-imaging were used to analyze samples of tibial and peroneal human tendons from ECMO and non-ECMO donors. The results showed significant differences in the chemical composition and extracellular matrix characteristics between tendons from these two groups, indicating changes in amino acid content and protein structure.

JOURNAL OF BIOPHOTONICS (2023)

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Article Biochemistry & Molecular Biology

Untargeted Lipidomic Approach for Studying Different Nervous System Tissues of the Murine Model of Krabbe Disease

Husam B. R. Alabed, Ambra Del Grosso, Valeria Bellani, Lorena Urbanelli, Sara Carpi, Miriam De Sarlo, Lorenzo Bertocci, Laura Colagiorgio, Sandra Buratta, Luca Scaccini, Dorotea Frongia Mancini, Ilaria Tonazzini, Marco Cecchini, Carla Emiliani, Roberto Maria Pellegrino

Summary: This study compared the lipid profiles of nervous system tissues in Twitcher mice and wild-type mice, revealing significant differences between the two groups, particularly in the sciatic nerve. The in-depth study of the lipid phenotype provided insights into the genes and enzymes involved in the disease.

BIOMOLECULES (2023)

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Article Chemistry, Multidisciplinary

Electrospun Nanofibrous UV Filters with Bidirectional Actuation Properties Based on Salmon Sperm DNA/Silk Fibroin for Biomedical Applications

Maria Rachele Ceccarini, Irene Chiesa, Francesca Ripanti, Martina Alunni Cardinali, Simone Micalizzi, Gabriele Scattini, Carmelo De Maria, Alessandro Paciaroni, Caterina Petrillo, Lucia Comez, Matteo Bertelli, Paola Sassi, Luisa Pascucci, Tommaso Beccari, Luca Valentini

Summary: In this study, a bioinspired spinning process was used to produce an electrospun nanofibrous silk-based patch. The patch demonstrated bidirectional macroscale actuation behavior in response to water vapor and displayed excellent UV-blocking properties. By incorporating DNA, the patch showed enhanced electrical conductivity and water-insolubility. Biological experiments confirmed that the patch provided photoprotection and improved cellular viability after UV exposure. Moreover, the patch treated with water vapor exhibited potential application as a scaffold for tissue engineering and promoted tissue regeneration when combined with HaCaT cells.

ACS OMEGA (2023)

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Proceedings Paper Engineering, Biomedical

Detection and identification by vibrational spectroscopy of myocardial biochemical alterations in Heart Failure with Preserved Ejection Fraction

L. Pioppi, R. Parvan, A. Samrend, G. Justo da Silva, M. Paolantoni, A. Cataliotti, P. Sassi

Summary: HFpEF is a complex cardiovascular disease with unknown progression mechanism and challenging diagnosis. This study used FTIR and Raman spectroscopies to detect biochemical alterations related to HFpEF and its associated comorbidities in the rat ventricles, demonstrating the high sensitivity and selectivity of these techniques in detecting changes in heart chemistry.

TRANSLATIONAL BIOPHOTONICS: DIAGNOSTICS AND THERAPEUTICS III (2023)

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Proceedings Paper Engineering, Biomedical

Vibrational spectroscopy techniques for the study of cardiorenal syndrome in rat models

Sara Stefani, Martina Alunni Cardinali, Alessandro Cataliotti, Gustavo Jose Justo Silva, Reza Parvan, Marco Paolantoni, Paola Sassi

Summary: Vibrational spectroscopy is a powerful tool for analyzing molecular structures, particularly in the fields of biomedical and biophysical research. Fourier-transform infrared (FTIR) spectroscopy, in particular, allows for fast biochemical imaging of biological tissues. However, its application in assessing heart and kidney lesions induced by cardiovascular diseases has been limited.

TRANSLATIONAL BIOPHOTONICS: DIAGNOSTICS AND THERAPEUTICS III (2023)

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Proceedings Paper Engineering, Biomedical

Using Brillouin and Raman microspectroscopy to diagnose musculoskeletal disorders: from characterizing healthy phenotypes to detecting human osteoarthritic lesions

Martina Alunni Cardinali, Marco Govoni, Dante Dallari, Leonardo Vivarelli, Matilde Tschon, Silvia Brogini, Maurizio Mattarelli, Silvia Caponi, Assunta Morresi, Paola Sassi, Daniele Fioretto

Summary: Brillouin and Raman microspectroscopy is a scattering technique that assesses the mechanical and chemical properties of tissues. It has gained attention in the biomedical field and has potential for in vivo applications. It is particularly relevant for studying musculoskeletal tissue and detecting degenerative diseases such as osteoarthritis.

TRANSLATIONAL BIOPHOTONICS: DIAGNOSTICS AND THERAPEUTICS III (2023)

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